AHR Antibody

Images

 
Genetic Strategies: Knockdown Validated: AHR Antibody [NB100-2289] - Activation of AHR/Nrf2 pathway by PFT-alpha. Knock-down efficiency of AHR siRNA as detected by qPCR (upper part) and western blot (lower part). ...read more
Immunohistochemistry: AHR Antibody [NB100-2289] - Staining of Aryl hydrocarbon Receptor in mouse prostate.
Immunocytochemistry/ Immunofluorescence: AHR Antibody [NB100-2289] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were ...read more
Western Blot: AHR Antibody [NB100-2289] - Detection of AhR in mouse liver cytosol using NB 100-2289.
Flow Cytometry: AHR Antibody [NB100-2289] - An intracellular stain was performed on U-87 cells with AHR Antibody NB100-2289B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. ...read more
Immunocytochemistry/ Immunofluorescence: AHR Antibody [NB100-2289] - Aryl hydrocarbon Receptor antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Simple Western: AHR Antibody [NB100-2289] - Image shows a specific band for Aryl hydrocarbon receptor in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, GpSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

AHR Antibody Summary

Immunogen
Bacterially expressed human Aryl hydrocarbon Receptor (C-terminus). [UniProt# P35869]
Localization
First cytoplasmic, upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AHR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Simple Western 1:200
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 2.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
Application Notes
In Western blot a band is seen ~90 to 105 kDa representing AHR (molecular weight varies by species and by strain). Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
AHR Overexpression Lysate
Mouse Liver Whole Tissue Lysate (Adult Whole Normal)
AHR Knockout HeLa Cell Lysate
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-2289 in the following applications:

Publications
Read Publications using
NB100-2289 in the following applications:

  • 1 publication
  • WB
    5 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23887904). Porcine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AHR Antibody

  • Ah receptor
  • AHR
  • AH-receptor
  • aryl hydrocarbon receptor
  • BHLHE76
  • bHLHe76aromatic hydrocarbon receptor
  • Class E basic helix-loop-helix protein 76

Background

Aryl hydrocarbon Receptor (AhR) is a ligand-activated transcriptional activator that binds to XRE promoter region of its target genes and has been established to activate phase I/II xenobiotic biotransformation enzyme genes. AhR binds MYBBP1A and its efficient DNA binding requires dimerization with another bHLH proteinsuch as ARNT. Besides interacting with NEDD8 and IVNS1ABP, AhR also interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT. AhR localize to cytoplasm and upon binding with ligand followed by interaction with a HSP90, it translocates to the nucleus. AhR has been shown to be induced or repressed by TGFB1 as well as dioxin in a cell-type specific fashion, whereas cAMP, retinoic acid, TPA (12-O-tetradecanoyl phorbol-13 acetate) have been shown to exert repressive effects on AhR. As a member of bHLH-PAS (basic helix-loop-helix Per/ARNT/Sim) family, AhR has been implicated in physiological processes such as circadian cycle, neurogenesis, organs development and regulation of cell-cycle as well as the alternative signaling pathways (independently of exposure to xenobiotics). AhR-null mice exhibit infertility, liver abnormalities and cardiovascular problems such as a defective closure of the ductus venosus, indicative of the implication of the AhR in various developmental processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AHR Antibody (NB100-2289)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: Flow, WB.


Filter By Application
Flow
(1)
WB
(5)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
Rat
(3)
All Species

Reviews for AHR Antibody (NB100-2289) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Other, Pig.

Reviews using NB100-2289:
Filter by Applications
IHC-P
(1)
ELISA
(1)
All Applications
Filter by Species
Other
(1)
Pig
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin AHR NB100-2289
Enlarge
5
reviewed by:
Anonymous
IHC-P Pig 10/26/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedlung
SpeciesPig
LotE-1
  4
reviewed by:
Anonymous
ELISA Other 06/04/2009
View

Summary

ApplicationELISA
Sample TestedGuinea Pig Liver Extract
SpeciesOther
LotA
CommentsThis was used for Direct ELISA assay.

Primary Anitbody

Dilution RatioIncubation Dilution Buffer: Tris/NaCl, Dilution Ratio: 1/500, Incubation Time: 1 hour, Incubation Temp: 70 degrees Fahrenheit

Comments

CommentsThis was used for Direct ELISA assay.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AHR Antibody (NB100-2289) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2289AF350
Alexa Fluor 405 NB100-2289AF405
Alexa Fluor 488 NB100-2289AF488
Alexa Fluor 532 NB100-2289AF532
Alexa Fluor 594 NB100-2289AF594
Alexa Fluor 647 NB100-2289AF647
Alexa Fluor 700 NB100-2289AF700
Alexa Fluor 750 NB100-2289AF750
Biotin NB100-2289B
DyLight 350 NB100-2289UV
DyLight 405 NB100-2289V
DyLight 488 NB100-2289G
DyLight 550 NB100-2289R
DyLight 594 NB100-2289DL594
DyLight 650 NB100-2289C
DyLight 680 NB100-2289FR
DyLight 755 NB100-2289IR
FITC NB100-2289F
HRP NB100-2289H
Janelia Fluor 549 NB100-2289JF549
Janelia Fluor 646 NB100-2289JF646

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Bioinformatics Tool for AHR Antibody (NB100-2289)

Discover related pathways, diseases and genes to AHR Antibody (NB100-2289). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for AHR Antibody (NB100-2289)

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Pathways for AHR Antibody (NB100-2289)

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Research Areas for AHR Antibody (NB100-2289)

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Blogs on AHR.

AHR - A transcription factor regulating immunity and tumorigenesis
The aryl hydrocarbon receptor (AHR) is a ligand activated transcription factor that controls the expression of a diverse set of genes. In the absence of ligand, AHR is retained in the cytoplasm. Upon ligand binding AHR translocates to the nucleus w...  Read full blog post.

Aryl Hydrocarbon Signaling: AIP, AhR, ARNT, BMAL1 and more...
AH receptor-interacting protein (AIP) is a 37 kD immunophilin-like factor found in a variety of tissues with expression levels ranging from high (spleen, thymus, pituitary heart, placenta and skeletal muscle) to low (liver, kidney and lung). It mediat...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
10/26/2020
Application: IHC-P
Species: Pig

 
Anonymous
06/04/2009
Application: ELISA
Species: Other

Bioinformatics

Gene Symbol AHR