AHR Antibody


Western Blot: AHR Antibody [NB100-2289] - Detection of AhR in mouse liver cytosol using NB 100-2289.
Immunocytochemistry/ Immunofluorescence: AHR Antibody [NB100-2289] - Aryl hydrocarbon Receptor antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Immunohistochemistry: AHR Antibody [NB100-2289] - Staining of Aryl hydrocarbon Receptor in mouse prostate.
Simple Western: AHR Antibody [NB100-2289] - Simple Western lane view shows a specific band for Aryl hydrocarbon receptor in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Reactivity Hu, Mu, Rt, GPSpecies Glossary
Applications WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P, IP
1.0 mg/ml

AHR Antibody Summary

Bacterially expressed human Aryl hydrocarbon Receptor (C-terminus). [UniProt# P35869]
First cytoplasmic, upon binding with ligand and interaction with a HSP90, it translocates to the nucleus
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


  • Western Blot 1:500-1:2000
  • Simple Western 1:200
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This Aryl hydrocarbon Receptor antibody is useful for Immunohistochemistry-paraffin embedded sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, ELISA and Western blot. In Western blot a band is seen ~90 to 105 kDa representing AHR (molecular weight varies by species and by strain). In ICC/IF, cytoplasmic staining was observed in MCF-7 cells. In IHC-P, staining was observed in the cytoplasm and nucleus of mouse prostate. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
NB100-2289 in the following applications:

Read Publication using
NB100-2289 in the following applications:

  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23887904)

Alternate Names for AHR Antibody

  • Ah receptor
  • AhR
  • AH-receptor
  • aryl hydrocarbon receptor
  • BHLHE76
  • bHLHe76aromatic hydrocarbon receptor
  • Class E basic helix-loop-helix protein 76


Aryl hydrocarbon Receptor (AhR) is a ligand-activated transcriptional activator that binds to XRE promoter region of its target genes and has been established to activate phase I/II xenobiotic biotransformation enzyme genes. AhR binds MYBBP1A and its efficient DNA binding requires dimerization with another bHLH proteinsuch as ARNT. Besides interacting with NEDD8 and IVNS1ABP, AhR also interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT. AhR localize to cytoplasm and upon binding with ligand followed by interaction with a HSP90, it translocates to the nucleus. AhR has been shown to be induced or repressed by TGFB1 as well as dioxin in a cell-type specific fashion, whereas cAMP, retinoic acid, TPA (12-O-tetradecanoyl phorbol-13 acetate) have been shown to exert repressive effects on AhR. As a member of bHLH-PAS (basic helix-loop-helix Per/ARNT/Sim) family, AhR has been implicated in physiological processes such as circadian cycle, neurogenesis, organs development and regulation of cell-cycle as well as the alternative signaling pathways (independently of exposure to xenobiotics). AhR-null mice exhibit infertility, liver abnormalities and cardiovascular problems such as a defective closure of the ductus venosus, indicative of the implication of the AhR in various developmental processes.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AHR Antibody (NB100-2289)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.

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Reviews for AHR Antibody (NB100-2289) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: ELISA.

Reviews using NB100-2289:
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Images Ratings Applications Species Date Details
reviewed by:
ELISA Other 06/04/2009


Sample TestedGuinea Pig Liver Extract
CommentsThis was used for Direct ELISA assay.

Primary Anitbody

Dilution RatioIncubation Dilution Buffer: Tris/NaCl, Dilution Ratio: 1/500, Incubation Time: 1 hour, Incubation Temp: 70 degrees Fahrenheit


CommentsThis was used for Direct ELISA assay.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AHR Antibody (NB100-2289) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-2289AF405
Alexa Fluor (R) 488 Labeled NB100-2289AF488
Alexa Fluor (R) 647 Labeled NB100-2289AF647
Alexa Fluor (R) 700 Labeled NB100-2289AF700
Biotin Labeled NB100-2289B
DyLight 405 Labeled NB100-2289V
DyLight 488 Labeled NB100-2289G
DyLight 550 Labeled NB100-2289R
DyLight 650 Labeled NB100-2289C
FITC Labeled NB100-2289F
HRP Labeled NB100-2289H

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Blogs on AHR.

AHR - A transcription factor regulating immunity and tumorigenesis
The aryl hydrocarbon receptor (AHR) is a ligand activated transcription factor that controls the expression of a diverse set of genes. In the absence of ligand, AHR is retained in the cytoplasm. Upon ligand binding AHR translocates to the nucleus w...  Read full blog post.

Aryl Hydrocarbon Signaling: AIP, AhR, ARNT, BMAL1 and more...
AH receptor-interacting protein (AIP) is a 37 kD immunophilin-like factor found in a variety of tissues with expression levels ranging from high (spleen, thymus, pituitary heart, placenta and skeletal muscle) to low (liver, kidney and lung). It mediat...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol AHR

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