AHR Antibody

Western Blot: AHR Antibody [NB100-2289] - Detection of AhR in mouse liver cytosol using NB 100-2289.

Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-2289] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-AHR at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: AHR Antibody [NB100-2289] - Staining of Aryl hydrocarbon Receptor in mouse prostate.

Flow Cytometry: AHR Antibody [NB100-2289] - An intracellular stain was performed on U-87 cells with AHR Antibody NB100-2289B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).

Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-2289] - Aryl hydrocarbon Receptor antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Simple Western: AHR Antibody [NB100-2289] - Image shows a specific band for Aryl hydrocarbon receptor in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Gp
• Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

AHR Antibody Summary

• Immunogen: Bacterially expressed human Aryl hydrocarbon Receptor (C-terminus). [UniProt# P35869]
• Localization: First cytoplasmic, upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AHR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000
  • Simple Western 1:200
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 2.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:1000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: In Western blot a band is seen ~90 to 105 kDa representing AHR (molecular weight varies by species and by strain). Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  AHR Overexpression Lysate
  Mouse Liver Whole Tissue Lysate (Adult Whole Normal)
  AHR Knockout HeLa Cell Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23887904).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AHR Antibody

• Ah receptor
• AHR
• AH-receptor
• aryl hydrocarbon receptor
• BHLHE76
• bHLHe76aromatic hydrocarbon receptor
• Class E basic helix-loop-helix protein 76

Background

Aryl hydrocarbon Receptor (AhR) is a ligand-activated transcriptional activator that binds to XRE promoter region of its target genes and has been established to activate phase I/II xenobiotic biotransformation enzyme genes. AhR binds MYBBP1A and its efficient DNA binding requires dimerization with another bHLH proteinsuch as ARNT. Besides interacting with NEDD8 and IVNS1ABP, AhR also interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT. AhR localize to cytoplasm and upon binding with ligand followed by interaction with a HSP90, it translocates to the nucleus. AhR has been shown to be induced or repressed by TGFB1 as well as dioxin in a cell-type specific fashion, whereas cAMP, retinoic acid, TPA (12-O-tetradecanoyl phorbol-13 acetate) have been shown to exert repressive effects on AhR. As a member of bHLH-PAS (basic helix-loop-helix Per/ARNT/Sim) family, AhR has been implicated in physiological processes such as circadian cycle, neurogenesis, organs development and regulation of cell-cycle as well as the alternative signaling pathways (independently of exposure to xenobiotics). AhR-null mice exhibit infertility, liver abnormalities and cardiovascular problems such as a defective closure of the ductus venosus, indicative of the implication of the AhR in various developmental processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AHR Antibody (NB100-2289)(5)

Publications using NB100-2289

• Zhu J, Singh M, Selivanova G, Peuget S Pifithrin-alpha alters p53 post-translational modifications pattern and differentially inhibits p53 target genes Sci Rep Jan 23 2020 [PMID: 31974452] (WB, Human)
• Hsu CN, Lin YJ, Tain YL Maternal Exposure to Bisphenol A Combined with High-Fat Diet-Induced Programmed Hypertension in Adult Male Rat Offspring: Effects of Resveratrol Int J Mol Sci Sep 6 2019 [PMID: 31489946] (WB, Rat)
• Meng Y, Lin R, Wu F et al. Decreased Capacity for Sperm Production Induced by Perinatal Bisphenol A Exposure Is Associated with an Increased Inflammatory Response in the Offspring of C57BL/6 Male Mice. Int J Environ Res Public Health. Oct 1 2018 [PMID: 30275377] (WB, Mouse)
• Hsu CN, Lin YJ, Lu PC, Tain YL. Maternal Resveratrol Therapy Protects Male Rat Offspring against Programmed Hypertension Induced by TCDD and Dexamethasone Exposures: Is It Relevant to Aryl Hydrocarbon Receptor. Int J Mol Sci Aug 20 2018 [PMID: 30127255] (WB, Rat)
• Bradley JM, Cryar KA, El Hajj MC et al. Exposure to Diesel Exhaust Particulates Induces Cardiac Dysfunction and Remodeling. J Appl Physiol 2013 Jul 25 [PMID: 23887904] (WB, Rat)

Review for AHR Antibody (NB100-2289) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Anonymous ELISA Other 06/04/2009

Summary

• Application: ELISA
• Sample Tested: Guinea Pig Liver Extract
• Species: Other
• Lot: A
• Comments: This was used for Direct ELISA assay.

Primary Anitbody

• Dilution Ratio: Incubation Dilution Buffer: Tris/NaCl, Dilution Ratio: 1/500, Incubation Time: 1 hour, Incubation Temp: 70 degrees Fahrenheit

Comments

• Comments: This was used for Direct ELISA assay.

Bioinformatics

• Gene Symbol: AHR