AHR Antibody - BSA Free

Images

 
Genetic Strategies: Knockdown Validated: AHR Antibody [NB100-2289] - Activation of AHR/Nrf2 pathway by PFT-alpha. Knock-down efficiency of AHR siRNA as detected by qPCR (upper part) and western blot (lower part). ...read more
Immunohistochemistry: AHR Antibody [NB100-2289] - Staining of Aryl hydrocarbon Receptor in mouse prostate.
Immunocytochemistry/ Immunofluorescence: AHR Antibody [NB100-2289] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were ...read more
Western Blot: AHR Antibody [NB100-2289] - Detection of AhR in mouse liver cytosol using NB 100-2289.
Immunocytochemistry/ Immunofluorescence: AHR Antibody [NB100-2289] - HepG2 cells were fixed for 10 minutes using 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Flow Cytometry: AHR Antibody [NB100-2289] - An intracellular stain was performed on U937 cells with NB100-2289 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then ...read more
Immunocytochemistry/ Immunofluorescence: AHR Antibody [NB100-2289] - Aryl hydrocarbon Receptor antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Flow Cytometry: AHR Antibody [NB100-2289] - An intracellular stain was performed on U-87 cells with AHR Antibody NB100-2289B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. ...read more
Simple Western: AHR Antibody [NB100-2289] - Image shows a specific band for Aryl hydrocarbon receptor in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, GpSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

AHR Antibody - BSA Free Summary

Immunogen
Bacterially expressed human Aryl hydrocarbon Receptor (C-terminus). [UniProt# P35869]
Localization
First cytoplasmic, upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AHR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 2.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Simple Western 1:200
  • Western Blot 1:500-1:2000
Application Notes
In Western blot a band is seen ~90 to 105 kDa representing AHR (molecular weight varies by species and by strain). Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
AHR Overexpression Lysate
Mouse Liver Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-2289 in the following applications:

Publications
Read Publications using
NB100-2289 in the following applications:

  • 1 publication
  • 1 publication
  • WB
    5 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23887904). Porcine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AHR Antibody - BSA Free

  • Ah receptor
  • AHR
  • AH-receptor
  • aryl hydrocarbon receptor
  • BHLHE76
  • bHLHe76aromatic hydrocarbon receptor
  • Class E basic helix-loop-helix protein 76

Background

Aryl hydrocarbon Receptor (AhR) is a ligand-activated transcriptional activator that binds to XRE promoter region of its target genes and has been established to activate phase I/II xenobiotic biotransformation enzyme genes. AhR binds MYBBP1A and its efficient DNA binding requires dimerization with another bHLH proteinsuch as ARNT. Besides interacting with NEDD8 and IVNS1ABP, AhR also interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT. AhR localize to cytoplasm and upon binding with ligand followed by interaction with a HSP90, it translocates to the nucleus. AhR has been shown to be induced or repressed by TGFB1 as well as dioxin in a cell-type specific fashion, whereas cAMP, retinoic acid, TPA (12-O-tetradecanoyl phorbol-13 acetate) have been shown to exert repressive effects on AhR. As a member of bHLH-PAS (basic helix-loop-helix Per/ARNT/Sim) family, AhR has been implicated in physiological processes such as circadian cycle, neurogenesis, organs development and regulation of cell-cycle as well as the alternative signaling pathways (independently of exposure to xenobiotics). AhR-null mice exhibit infertility, liver abnormalities and cardiovascular problems such as a defective closure of the ductus venosus, indicative of the implication of the AhR in various developmental processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AHR Antibody (NB100-2289)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ChIP, Flow, WB.


Filter By Application
ChIP
(1)
Flow
(1)
WB
(5)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Rat
(3)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-2289 Applications Species
Wang Y, Sun Q, Ye Y et al. FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis JCI insight [PMID: 35439170] (ChIP, Human) ChIP Human
Baban B, Braun M, Khodadadi H, et al. AMPK induces regulatory innate lymphoid cells after traumatic brain injury JCI insight Jan 11 2021 [PMID: 33427206] (Flow, Human) Flow Human
Zhu J, Singh M, Selivanova G, Peuget S Pifithrin-alpha alters p53 post-translational modifications pattern and differentially inhibits p53 target genes Sci Rep Jan 23 2020 [PMID: 31974452] (WB, Human) WB Human
Hsu CN, Lin YJ, Tain YL Maternal Exposure to Bisphenol A Combined with High-Fat Diet-Induced Programmed Hypertension in Adult Male Rat Offspring: Effects of Resveratrol Int J Mol Sci Sep 6 2019 [PMID: 31489946] (WB, Rat) WB Rat
Meng Y, Lin R, Wu F et al. Decreased Capacity for Sperm Production Induced by Perinatal Bisphenol A Exposure Is Associated with an Increased Inflammatory Response in the Offspring of C57BL/6 Male Mice. Int J Environ Res Public Health. Oct 1 2018 [PMID: 30275377] (WB, Mouse) WB Mouse
Hsu CN, Lin YJ, Lu PC, Tain YL. Maternal Resveratrol Therapy Protects Male Rat Offspring against Programmed Hypertension Induced by TCDD and Dexamethasone Exposures: Is It Relevant to Aryl Hydrocarbon Receptor. Int J Mol Sci Aug 20 2018 [PMID: 30127255] (WB, Rat) WB Rat
Bradley JM, Cryar KA, El Hajj MC et al. Exposure to Diesel Exhaust Particulates Induces Cardiac Dysfunction and Remodeling. J Appl Physiol 2013 Jul 25 [PMID: 23887904] (WB, Rat) WB Rat

Reviews for AHR Antibody (NB100-2289) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Other, Pig.

Reviews using NB100-2289:
Filter by Applications
Immunohistochemistry-Paraffin
(1)
ELISA
(1)
All Applications
Filter by Species
Other
(1)
Pig
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin AHR NB100-2289
Enlarge
5
reviewed by:
Beth Junecko
Immunohistochemistry-Paraffin Pig 10/26/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedlung
SpeciesPig
LotE-1
  4
reviewed by:
System Admin
ELISA Other 06/04/2009
View

Summary

ApplicationELISA
Sample TestedGuinea Pig Liver Extract
SpeciesOther
LotA

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AHR Antibody (NB100-2289) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional AHR Products

Bioinformatics Tool for AHR Antibody (NB100-2289)

Discover related pathways, diseases and genes to AHR Antibody (NB100-2289). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for AHR Antibody (NB100-2289)

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Pathways for AHR Antibody (NB100-2289)

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Research Areas for AHR Antibody (NB100-2289)

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Blogs on AHR.

AHR - A transcription factor regulating immunity and tumorigenesis
The aryl hydrocarbon receptor (AHR) is a ligand activated transcription factor that controls the expression of a diverse set of genes. In the absence of ligand, AHR is retained in the cytoplasm. Upon ligand binding AHR translocates to the nucleus w...  Read full blog post.

Aryl Hydrocarbon Signaling: AIP, AhR, ARNT, BMAL1 and more...
AH receptor-interacting protein (AIP) is a 37 kD immunophilin-like factor found in a variety of tissues with expression levels ranging from high (spleen, thymus, pituitary heart, placenta and skeletal muscle) to low (liver, kidney and lung). It mediat...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Beth Junecko
10/26/2020
Application: Immunohistochemistry-Paraffin
Species: Pig

 
System Admin
06/04/2009
Application: ELISA
Species: Other

Bioinformatics

Gene Symbol AHR