AHR Antibody [DyLight 550] Summary
Bacterially expressed human Aryl hydrocarbon Receptor (C-terminus). [UniProt# P35869]
First cytoplasmic, upon binding with ligand and interaction with a HSP90, it translocates to the nucleus
Immunogen affinity purified
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- Western Blot
This Aryl hydrocarbon Receptor antibody is useful for Immunohistochemistry-paraffin embedded sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, ELISA and Western blot. In Western blot a band is seen ~90 to 105 kDa representing AHR (molecular weight varies by species and by strain). In ICC/IF, cytoplasmic staining was observed in MCF-7 cells. In IHC-P, staining was observed in the cytoplasm and nucleus of mouse prostate. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Rat reactivity reported in scientific literature (PMID: 23887904)
Packaging, Storage & Formulations
Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Immunogen affinity purified
Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for AHR Antibody [DyLight 550]
- Ah receptor
- aryl hydrocarbon receptor
- bHLHe76aromatic hydrocarbon receptor
- Class E basic helix-loop-helix protein 76
The Ah Receptor belongs to a family of proteins comprised of its dimerization partner ARNT (HIF-1 Beta) and the Drosophila proteins PER and SIM. All of these proteins share an N-terminal sequence of ~200 amino acids termed the PAS domain. This receptor, found in a variety of tissues, binds to a specific DNA enhancer sequence and initiates transcription of the mRNA for the cytochrome P-450 (CYPIA1) gene.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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