Cytochrome P450 1A2 Antibody (OTI8F1)

Western Blot: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 1A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 1A2.

Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP1A2 .

Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.

Flow Cytometry: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cytochrome P450 1A2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Human colon tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Human lung tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Human pancreas tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Human prostate tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.

Flow Cytometry: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Analysis of Jurkat cells, using anti-Cytochrome P450 1A2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI8F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.77 mg/ml

Cytochrome P450 1A2 Antibody (OTI8F1) Summary

• Immunogen: Full length human recombinant protein of human CYP1A2 (NP_000752) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYP1A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 58.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.77 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cytochrome P450 1A2 Antibody (OTI8F1)

• aryl hydrocarbon hydroxylase
• CP12
• CYP1A2
• CYPIA2
• Cytochrome P(3)450
• Cytochrome P450 1A2
• Cytochrome P450 4
• cytochrome P450, family 1, subfamily A, polypeptide 2
• cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2
• Cytochrome P450-P3
• dioxin-inducible P3-450
• EC 1.14.14.1
• flavoprotein-linked monooxygenase
• microsomal monooxygenase
• P3-450
• P450 form 4
• P450(PA)
• xenobiotic monooxygenase

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytochrome P450 1A2 Antibody (NBP2-02563)(1)

Publication using NBP2-02563

• Wuensch T, Heucke N, Wizenty J et al. Hepatic CYP1A2 activity in liver tumors and the implications for preoperative volume-function analysis American Journal of Physiology-Gastrointestinal and Liver Physiology 2019-05-01 [PMID: 30869529] (ICC/IF)

Bioinformatics

• Gene Symbol: CYP1A2
• Entrez:
  • 1544(Human)