AHR Antibody [FITC] Summary
Bacterially expressed human Aryl hydrocarbon Receptor (C-terminus). [UniProt# P35869]
First cytoplasmic, upon binding with ligand and interaction with a HSP90, it translocates to the nucleus
Immunogen affinity purified
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This Aryl hydrocarbon Receptor antibody is useful for Immunohistochemistry-paraffin embedded sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, ELISA and Western blot. In Western blot a band is seen ~90 to 105 kDa representing AHR (molecular weight varies by species and by strain). In ICC/IF, cytoplasmic staining was observed in MCF-7 cells. In IHC-P, staining was observed in the cytoplasm and nucleus of mouse prostate. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Rat reactivity reported in scientific literature (PMID: 23887904)
Packaging, Storage & Formulations
Store at 4C in the dark.
0.05% Sodium Azide
Immunogen affinity purified
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for AHR Antibody [FITC]
- Ah receptor
- aryl hydrocarbon receptor
- bHLHe76aromatic hydrocarbon receptor
- Class E basic helix-loop-helix protein 76
Aryl hydrocarbon Receptor (AhR) is a ligand-activated transcriptional activator that binds to XRE promoter region of its target genes and has been established to activate phase I/II xenobiotic biotransformation enzyme genes. AhR binds MYBBP1A and its efficient DNA binding requires dimerization with another bHLH proteinsuch as ARNT. Besides interacting with NEDD8 and IVNS1ABP, AhR also interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT. AhR localize to cytoplasm and upon binding with ligand followed by interaction with a HSP90, it translocates to the nucleus. AhR has been shown to be induced or repressed by TGFB1 as well as dioxin in a cell-type specific fashion, whereas cAMP, retinoic acid, TPA (12-O-tetradecanoyl phorbol-13 acetate) have been shown to exert repressive effects on AhR. As a member of bHLH-PAS (basic helix-loop-helix Per/ARNT/Sim) family, AhR has been implicated in physiological processes such as circadian cycle, neurogenesis, organs development and regulation of cell-cycle as well as the alternative signaling pathways (independently of exposure to xenobiotics). AhR-null mice exhibit infertility, liver abnormalities and cardiovascular problems such as a defective closure of the ductus venosus, indicative of the implication of the AhR in various developmental processes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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