| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-92243SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78259SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-648SS | |||
| Azide and BSA Free | NBP2-81006 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27399 | |||
| Azide and BSA Free | NBP2-27399SS | |||
| BSA Free | NB100-56749SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80877 | |||
| BSA Free | NB110-81529SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-412SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56725SS | |||
| Azide and BSA Free | NBP2-33248 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-585SS | |||
| Azide and BSA Free | NBP2-80976 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-78486SS | |||
| Azide and BSA Free | NBP2-80884 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88845-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88932-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-28904SS | |||
![Western Blot: TfR (Transferrin R) Antibody [NB100-92243] - Analysis of TfR1 pAb at 1:500 dilution. (1) HEK293T whole cell lysate, (2) Raw264.7 whole cell lysate, and (3) H9C2 whole cell lysate.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Western-Blot-NB100-92243-img0006.jpg "Western Blot: TfR (Transferrin R) Antibody [NB100-92243] - Analysis of TfR1 pAb at 1:500 dilution. (1) HEK293T whole cell lysate, (2) Raw264.7 whole cell lysate, and (3) H9C2 whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody [NB100-92243] - Transferrin Receptor Antibody [NB100-92243] - Transferrin receptor antibody was tested at 1:50 in HeLa cells with DylLght 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/Transferrin-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NB100-92243-img0004.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody - BSA Free [NB100-92243] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of TfR (Transferrin R) antibody NB100-92243. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-BSA-Free-Immunohistochemistry-Paraffin-NB100-92243-img0007.jpg)
![Western Blot: TfR (Transferrin R) Antibody [NB100-92243] - Analysis of Transferrin Receptor Antibody in extracts from 293 cells.](http://images.novusbio.com/fullsize/Transferrin-Receptor-Antibody-Western-Blot-NB100-92243-img0001.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody [NB100-92243] - Paraffin-embedded human colorectal carcinoma tissue at 1:50.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-Immunohistochemistry-Paraffin-NB100-92243-img0005.jpg)
![Immunohistochemistry: GLI-1 Antibody [NBP1-78259] - In vivo GLI1 expression after intratumoral administration of NVP-LDE225. LOX OMVI human melanoma cells were injected s.c into both flanks. Tumors were treated intratumorally on daily basis with vehicle (A & B) or NVP-LDE225 (C & D). Immunofluorescent microscopy of GLI1 was performed on isolated tumor tissues. GLI1 staining was performed by overnight incubation of sections at 4C with rabbit anti-GLI-1 polyclonal Ab or isotype control (A & C) followed by an 1 hr-incubation with AF488 Donkey IgG, anti-rabbit at RT (green). Counterstaining of nuclei was performed with propidium iodide (red). Pictures were taken on a confocal laser-scanning microscope system (LSM 410; Zeiss). Yellow color corresponds to double positive (anti-GLI1 and propidium iodide) nuclear staining. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0069064) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLI-1-Antibody-Immunohistochemistry-NBP1-78259-img0009.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry: GLI-1 Antibody [NBP1-78259] - In vivo GLI1 expression after intratumoral administration of NVP-LDE225. LOX OMVI human melanoma cells were injected s.c into both flanks. Tumors were treated intratumorally on daily basis with vehicle (A & B) or NVP-LDE225 (C & D). Immunofluorescent microscopy of GLI1 was performed on isolated tumor tissues. GLI1 staining was performed by overnight incubation of sections at 4C with rabbit anti-GLI-1 polyclonal Ab or isotype control (A & C) followed by an 1 hr-incubation with AF488 Donkey IgG, anti-rabbit at RT (green). Counterstaining of nuclei was performed with propidium iodide (red). Pictures were taken on a confocal laser-scanning microscope system (LSM 410; Zeiss). Yellow color corresponds to double positive (anti-GLI1 and propidium iodide) nuclear staining. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0069064) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: GLI-1 Antibody [NBP1-78259] - Analysis of GLI-1 on partial recombinant GLI-1 protein (molecular weight of partial recombinant protein is 37 kDa).](http://images.novusbio.com/fullsize/GLI-1-Antibody-Western-Blot-NBP1-78259-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-1 Antibody [NBP1-78259] - GLI1 antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/GLI-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78259-img0006.jpg)
![Immunohistochemistry: GLI-1 Antibody [NBP1-78259] - Immunostaining of GLI-1 (green, indicated by arrows) in the suture mesenchyme of Bmpr1-alpha-fl/fl (control) and GLI1-CreERT2; Bmpr1-alpha-fl/fl (mutant) mice 2 weeks post induction (2 wpt). The inset shows the boxed region magnified. T tests were performed. *P 0.05. C) Immunofluorescence staining for Ki67 (red) and visualization of EdU (green) in dermal fibroblasts after co‐culture within the supernatant of sti‐RAW 264.7 cells or RAW 264.7 cells without LPS stimulation supplemented with vector or 1 um SAG for 48 h. D) Representative images and E) quantification of the results of the in vitro scratch assay of dermal fibroblasts co‐cultured within the supernatant of sti‐RAW 264.7 cells or RAW 264.7 cells without LPS stimulation supplemented with vector or 1 um SAG for 48 h. Dotted lines indicate the initial scratch edges. Data are presented as the mean +/- SD, n = 4. P‐values were calculated using ANOVA with Tukey's multiple comparisons test. *P 0.05. F) Representative images and G) quantification of tube formation in HUVECs co‐cultured within the supernatant of sti‐RAW 264.7 cells or RAW 264.7 cells without LPS stimulation supplemented with vector or 1 um SAG for 2 h. Data are presented as the mean +/- SD, n = 5. P‐values were calculated using ANOVA with Tukey's multiple comparisons test, *P 0.05. Scale bar, 200 um. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39413023), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78259_rabbit-polyclonal-gli-1-antibody-western-blot-132202618151523.jpg)
![Immunohistochemistry-Paraffin: VEGF Antibody (VG76e) [NB100-648] - IHC analysis of VEGF in fetal sheep brain tissue. Image courtesy of anonymous customer review.](http://images.novusbio.com/fullsize/VEGF-Antibody-VG76e-Immunohistochemistry-Paraffin-NB100-648-img0004.jpg "Immunohistochemistry-Paraffin: VEGF Antibody (VG76e) [NB100-648] - IHC analysis of VEGF in fetal sheep brain tissue. Image courtesy of anonymous customer review.")
![Western Blot: VEGF Antibody (VG76e) [NB100-648] - Analysis of VEGF in human kidney protein (30ug).](http://images.novusbio.com/fullsize/VEGF-Antibody-VG76e-Western-Blot-NB100-648-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: VEGF Antibody (VG76e) [NB100-648] - Immunofluorescent analysis of VEGF using NB100-648. This image submitted through a verified customer review.](http://images.novusbio.com/fullsize/VEGF-Antibody-VG76e-Immunocytochemistry-Immunofluorescence-NB100-648-img0007.jpg)
![Immunohistochemistry-Paraffin: VEGF Antibody (VG76e) [NB100-648] - IHC-P analysis of VEGF in sheep tonsil using NB100-648. This image was submitted through a verified customer review.](http://images.novusbio.com/fullsize/VEGF-Antibody-VG76e-Immunohistochemistry-Paraffin-NB100-648-img0006.jpg)
![Western Blot: Mouse Monoclonal VEGF Antibody (VG76e) [NB100-648] - Bone lysate homogenate from goats. SDS-PAGE with 0.3 mg of total protein from the homogenate per well. VEGF, NB100-648, was used at a dilution of 1:1000 in TBS Tween and 5% milk powder. The protein was labeled at 40 kDa. Quantification of VEGFA in bone graft in the tibia of intrailiac cellularized goats at different times compared with tibial autograft and untreated bone defect. Evaluation of pre-cellularized graft in iliac crest in the treatment of bone defects in the tibia of goats. G1: cellularized graft for 4 weeks applied to tibial bone defect, G2: cellularized graft for 6 weeks applied to tibial bone defect, G3: cellularized graft for 8 weeks applied to tibial bone defect, G4: tibial autograft and G5: untreated bone defect. All groups were evaluated 120 days after graft implantation. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-648_mouse-monoclonal-vegf-antibody-vg76e-western-blot-2952025142053..jpg)
![Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/mL) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-AKT1 (NBP2-01725) and 2 ug/mL pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Western-Blot-NB100-56749-img0014.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/mL) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-AKT1 (NBP2-01725) and 2 ug/mL pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - WB of phospho AKT using phospho AKT antibody at 2 ug/mL against untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate. HRP conjugated secondary antibody and ECL substrate solution were used for this test. Image using the Azide and BSA Free form of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Western-Blot-NB100-56749-img0015.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of an FFPE human breast carcinoma tissue section using 1:250 dilution of phospho Ser473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Immunohistochemistry-Paraffin-NB100-56749-img0009.jpg)
![Immunohistochemistry-Frozen: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Rat mammary carcinoma tissue section stained with AKT1 [p Ser473] Antibody (104A282). IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Immunohistochemistry-Frozen-NB100-56749-img0016.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of an FFPE human breast carcinoma tissue section using 1:250 dilution of pSer473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Some cancer cells depicted nuclear stianing also. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Immunohistochemistry-Paraffin-NB100-56749-img0010.jpg)
![EZH2/HMGA2 regulates the PI3K/AKT signaling. MI rats were subjected to injection of EZH2-OE or EZH2-NC (MI + MSC-EXO + EZH2 NC/EZH2 OE) and EZH2-OE + HMGA2-OE or EZH2-OE + HMGA2-NC. A HMGA2 protein expression in myocardial tissues in response to EZH2-OE + HMGA2-OE examined using western blot (Additional file 5: Fig. S5). B Weight change in rats after EZH2-OE + HMGA2-OE treatment. C Changes in HMI in rats after EZH2-OE + HMGA2-OE treatment. D Measurement of Dd, Sd, and LVEF by echocardiography at 2 weeks after LAD in rats. E Measurement of LVMI by echocardiography in rats. F KEGG pathway analysis of gene enrichment pathways [29]. G Changes in PI3K/AKT pathway in rat myocardium examined using western blot (Additional file 6: Fig. S6). All data are expressed as mean +/- SD (n = 6/group, *p < 0.05 vs. Sham, DMSO, MI + MSC-EXO + EZH2-NC or EZH2-OE + HMGA2-NC group determined by unpaired t test or one-way ANOVA) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35264108), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56749_mouse-monoclonal-akt1-p-ser473-antibody-104a282-western-blot-13220261820240.jpg)
![Western Blot: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Detection of NHE3 [p Ser552] in human kidney lysate.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-p-Ser552-Antibody-14D5-Western-Blot-NB110-81529-img0005.jpg "Western Blot: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Detection of NHE3 [p Ser552] in human kidney lysate.")
![Immunohistochemistry: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Mouse intestine, image courtesy of verified customer review.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-p-Ser552-Antibody-14D5-Immunofluorescence-NB110-81529-img0008.jpg)
![Simple Western: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Simple Western lane view shows a specific band for NHE3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-p-Ser552-Antibody-14D5-Simple-Western-NB110-81529-img0006.jpg)
![Western Blot: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K golgi protein expression in rat liver tissue using NB600-412.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Western-Blot-NB600-412-img0007.jpg "Western Blot: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K golgi protein expression in rat liver tissue using NB600-412.")
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - HeLa cells, cultured on cover slips, were fixed with 10% formalin for 10 minutes and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were then incubated with 1:200 dilution of anti-58K Golgi Protein antibody (clone 58K-9) for overnight at 4C and detected with an anti-mouse Dylight 488 (Green) secondary at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at 1:500 dilution. Nuclei were counterstained with DAPI solution (Blue) [NBP2-31156]. Cells were imaged using a 40X objective. Antibody clone 58K-9 generated a specific signal in the Golgi apparatus of the cells.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Immunocytochemistry-Immunofluorescence-NB600-412-img0010.jpg)
![Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412] - DMD muscle co-stained for Neu5Gc (green) with 58K Golgi, a Golgi marker, in red, and DAPI (blue). Arrow marks region of coincident staining (yellow) for Neu5Gc and 58K Golgi. Bar is 50 um. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0088226) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Immunohistochemistry-NB600-412-img0011.jpg)
![Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K Golgi Protein in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Immunohistochemistry-NB600-412-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Assessing Golgi morphology observed apoptosis in mBECs using Mouse 58K Golgi Protein at a dilution of 1:100 overnight at 4 °C. This image was taken on a Zeiss LSM 710 Confocal Microscope (META) with Zeiss Plan Apo 63x/1.40 oil. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nb600-412_mouse-monoclonal-58k-golgi-protein-antibody-58k-9-immunocytochemistry-immunofluorescence-204202310243.jpg)
![Immunocytochemistry/ Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Co-localization of Neu5Gc staining with markers for endosomes & Golgi in BMD & DMD muscle.(A) BMD muscle co-stained for Neu5Gc (green) & clathrin (red), a marker of endosomes. Merged image on right shows overlap of Neu5Gc & clathrin expression in yellow. Arrow marks several examples of co-staining. (B) DMD muscle co-stained for Neu5Gc (green) with LAMP1, a lysosomal marker, 58K Golgi, a Golgi marker, or calnexin, & endoplasmic reticulum marker, all in red, & DAPI (blue). Arrow marks region of coincident staining (yellow) for Neu5Gc & 58K Golgi. Bar is 50 µm for all panels in A & B. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24505439), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-412_mouse-monoclonal-58k-golgi-protein-antibody-58k-9-310202415304219.jpg)
![Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - WB analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (NB100-56725, Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI/RhoGDI2 cleavage was detected.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Western-Blot-NB100-56725-img0001.jpg "Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - WB analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (NB100-56725, Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI/RhoGDI2 cleavage was detected.")
![Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and this antibody.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Immunohistochemistry-Paraffin-NB100-56725-img0002.jpg)
![Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Intracellular analysis of cleaved D4-GDI in 10^6 untreated (left) and 3 hour staurosporine-treated (right) Jurkat cells using 0.5 ug of this antibody was used for this test. Green histogram represents isotype control; red represents anti-D4-GDI antibody. Image using the Alexa Fluor 647 format of this antibody.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Flow-Intracellular-NB100-56725-img0011.jpg)
![Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Western blot analysis of D4GDI in Jurkat cells using NB100-56556 (IMG-396A) at 2 ug/ml and NB100-56725 (IMG-160A) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 (IMG-396A) detects both the full-length and cleaved forms of D4GDI. While (NB100-56725) IMG-160A specifically detects only the cleaved form of the protein.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Western-Blot-NB100-56725-img0005.jpg)
![Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the (0.5 ug antibody/test/1 million cells) and ((as seen in B). this is because (cells not undergoing apoptosis) and cleaved (apoptotic cells) forms of D4-GDI.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Flow-Intracellular-NB100-56725-img0004.jpg)
![Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Analysis using the FITC conjugate of NB100-56725. Staining of cleaved D4-GDI in 10^6 camptothecin-treated (red) and untreated (green) HL60 cells using 0.5 ug of NB100-56060. The shaded histogram represents cells without antibody.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Flow-Intracellular-NB100-56725-img0009.jpg)
![Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Analysis using the FITC conjugate of NB100-56725. Staining of cleaved D4-GDI in untreated (left) and staurosporine-treated (right) Ramos cells using 0.5 ug of was used for this test.](http://images.novusbio.com/fullsize/D4-GDI-RhoGDI2-Antibody-97A1015-cleavage-specific-Flow-Intracellular-NB100-56725-img0010.jpg)
![Western Blot: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Total protein from rat adrenal gland cells (PC12) and rat placenta was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD71 in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Western-Blot-NB200-585-img0009.jpg "Western Blot: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Total protein from rat adrenal gland cells (PC12) and rat placenta was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD71 in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (OX26) [NB200-585] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti TfR/Transferrin R (OX26) NB200-585 at a 1:100 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (red) at a 1:200 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Immunocytochemistry-Immunofluorescence-NB200-585-img0007.jpg)
![Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Analysis of FFPE mouse spleen using TfR (OX26) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Plasma membrane staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Immunohistochemistry-Paraffin-NB200-585-img0012.jpg)
![Flow Cytometry: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Analysis using the Biotin conjugate of NB200-585. Staining of mouse spleen.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Flow-Cytometry-NB200-585-img0010.jpg)
![Flow Cytometry: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Staining of rat spleen lymphocytes with mouse anti rat CD71.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Flow-Cytometry-NB200-585-img0001.jpg)
![Flow (Intracellular): TfR (Transferrin R) Antibody (OX26) [NB200-585] - PC-12 cells were stained with NB200-585 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by DyLight488-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Flow-Intracellular-NB200-585-img0008.jpg)
![Flow Cytometry: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Staining of rat spleen cells with mouse anti rat CD71: RPE.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Flow-Cytometry-NB200-585-img0006.jpg)
![Flow Cytometry: TfR (Transferrin R) Antibody (OX26) [NB200-585] - An intracellular stain was performed on PC12 cells with TfR (Transferrin R) [OX26] Antibody NB200-585AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-OX26-Flow-Cytometry-NB200-585-img0013.jpg)
![Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/mL of Mouse Anti-Human Notch-1 Monoclonal Antibody (Catalog # NB100-78486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Notch-1 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Knockout-Validated-NB100-78486-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/mL of Mouse Anti-Human Notch-1 Monoclonal Antibody (Catalog # NB100-78486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Notch-1 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Cell extracts from Jurkat (Lane 1) or mouse thymocytes (Lane 2) were analyzed with monoclonal anti-NOTCH1 antibody. The mN1A antibody recognizes both mouse and human 270 kDa full-length NOTCH1 and 110-120 kDa cleaved NOTCH 1 (NICD).](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Western-Blot-NB100-78486-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Notch-1 Antibody (mN1A) [NB100-78486] - The Notch1 Antibody was tested in HEK293 cells at a 1:50 dilution with DyLight 488 (Green). Actin was counterstained with Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue).](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Immunocytochemistry-Immunofluorescence-NB100-78486-img0008.jpg)
![Immunohistochemistry-Paraffin: Notch-1 Antibody (mN1A) [NB100-78486] - Analysis of FFPE human pancreatic cancer using 1:10 dilution of Notch-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in epithelial cells was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Immunohistochemistry-Paraffin-NB100-78486-img0010.jpg)
![Flow Cytometry: Notch-1 Antibody (mN1A) [NB100-78486] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using Notch1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Notch-1-Antibody-mN1A-Flow-Cytometry-NB100-78486-img0007.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Analysis in human cerebral cortex and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Analysis in human cerebral cortex and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB1 Antibody [NBP1-88845] - Mesenchymal-high cancers exhibit enhanced sensitivity to ferroptosis inducers. Representative cell lines showing enhanced sensitivity to GPx4 inhibitor ML162 correlates to low levels of E-cadherin, an epithelial marker. H1975 and A549: lung cancer; KLE and Ishikawa: endometrial cancer. Antibodies against E-cadherin, ZEB1, vimentin, and MTDH were used to detect protein extracts from non-small-cell lung cancer H1975 and A549, and endometrial cancer cell lines KLE and Ishikawa. Beta-Actin was detected as a loading control. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC6746770/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Western-Blot-NBP1-88845-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody [NBP1-88845] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88845-img0010.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human prostate shows strong nuclear positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0020.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Immunohistochemical staining of human endometrium shows strong nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0008.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0011.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human cerebral cortex shows strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0015.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human glioma shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0017.jpg)
![Western Blot: ARID1A Antibody [NBP1-88932] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/ARID1A-Antibody-Western-Blot-NBP1-88932-img0015.jpg "Western Blot: ARID1A Antibody [NBP1-88932] - Analysis in human cell line HDLM-2.")
![Immunocytochemistry/Immunofluorescence: ARID1A Antibody [NBP1-88932] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ARID1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88932-img0012.jpg)
![Immunohistochemistry-Paraffin: ARID1A Antibody [NBP1-88932] - Immunohistochemical staining of human colorectal cancer shows weak to moderate nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/ARID1A-Antibody-Immunohistochemistry-Paraffin-NBP1-88932-img0010.jpg)
![Immunohistochemistry-Paraffin: ARID1A Antibody [NBP1-88932] - Immunohistochemical staining of human duodenum shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ARID1A-Antibody-Immunohistochemistry-Paraffin-NBP1-88932-img0006.jpg)
![Immunohistochemistry-Paraffin: ARID1A Antibody [NBP1-88932] - Immunohistochemical staining of human fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ARID1A-Antibody-Immunohistochemistry-Paraffin-NBP1-88932-img0007.jpg)
![Immunohistochemistry-Paraffin: ARID1A Antibody [NBP1-88932] - Immunohistochemical staining of human cerebral cortex shows moderate to strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/ARID1A-Antibody-Immunohistochemistry-Paraffin-NBP1-88932-img0008.jpg)
![Immunohistochemistry-Paraffin: ARID1A Antibody [NBP1-88932] - Immunohistochemical staining of human lymph node shows moderate nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/ARID1A-Antibody-Immunohistochemistry-Paraffin-NBP1-88932-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lgr5/GPR49 Antibody NBP1-28904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-28904-img0009.jpg "Immunocytochemistry/Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lgr5/GPR49 Antibody NBP1-28904 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Wnt-related genes show selective expression in the ZG of human adrenal. IHC localization of LGR5 in the ZG. IHC was performed on formalin-fixed, paraffin-embedded human adrenal sections (4 um) using a chromogen-based detection system (3,3'-Diaminobenzidine), which results in a positive brown staining. Pictures are representative of six normal adrenal sections that were used in our microarray study; four from primary hyperaldosteronism patients and two from pheochromocytoma patients. Scale bar, 500 um (low power) and 100 um (high power). C, capsule. Image collected and cropped by CiteAb from the following publication (//academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2015-1734), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-NBP1-28904-img0011.jpg)
![Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Analysis of human small intestine.](http://images.novusbio.com/fullsize/Lgr5-GPR49-Antibody-Immunohistochemistry-NBP1-28904-img0005.jpg)
![Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - BSA Free [NBP1-28904] - Wnt-related genes show selective expression in the ZG of human adrenal. A, Heat map of 37 genes (shown on the vertical axis), which are 5-fold or greater differentially expressed in ZG & ZF. Genes previously associated with the Wnt signaling pathway are highlighted in red. On the horizontal axis, each pair of ZG & ZF from the 20 adrenals are anonymized & numbered. Probe set & detailed microarray information are available from the National Center for Biotechnology Information Gene Expression Omnibus under accession number GSE64957. B, IHC localization of LGR5 & downstream Wnt signaling proteins in the ZG: from the canonical beta -catenin pathway, beta -catenin & LEF1, & from the noncanonical AP1/JUN pathway, c-JUN & p-c-JUN. IHC was performed on formalin-fixed, paraffin-embedded human adrenal sections (4 μm) using a chromogen-based detection system (3,3'-Diaminobenzidine), which results in a positive brown staining. Interestingly, beta -catenin staining was mostly membranous or cytoplasmic (rather than nuclear staining as highlighted by the arrows), indicating limited canonical Wnt activation. Pictures are representative of six normal adrenal sections that were used in our microarray study; four from primary hyperaldosteronism patients & two from pheochromocytoma patients. Scale bar, 500 μm (low power) & 100 μm (high power). C, capsule. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2015-1734), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28904_rabbit-polyclonal-lgr5-gpr49-antibody-31020241618943.jpg)
