TfR (Transferrin R) Antibody (OX26)

Images

 
Western Blot: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Total protein from rat adrenal gland cells (PC12) and rat placenta was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (OX26) [NB200-585] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. ...read more
Immunohistochemistry-Paraffin: TfR (Transferrin R) Antibody (OX26) [NB200-585] - IHC analysis of formalin fixed paraffin-embedded (FFPE) mouse spleen using TfR (OX26) antibody at 1:50 on a Bond Rx autostainer (Leica ...read more
Flow Cytometry: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Staining of rat spleen cells with MOUSE ANTI RAT CD71: RPE.
Flow Cytometry: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Staining of rat spleen lymphocytes with Mouse anti Rat CD71
Flow (Intracellular): TfR (Transferrin R) Antibody (OX26) [NB200-585] - PC-12 cells were stained with TfR (Transferrin R) Antibody (OX26) NB200-585 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Flow Cytometry: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Analysis using the Biotin conjugate of NB200-585. Staining of mouse spleen.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
OX26
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

TfR (Transferrin R) Antibody (OX26) Summary

Immunogen
PHA activated rat lymphocytes.
Marker
Recycling Endosome Marker
Specificity
Labels dividing cells of various types but also binds to a number of non-dividing normal tissues and endothelium of brain capillaries.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TFRC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
Use in IHC was reported in the scientific literature (PMID: 11818402).
Publications
Read Publications using
NB200-585 in the following applications:

Reactivity Notes

Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TfR (Transferrin R) Antibody (OX26)

  • CD71 antigen
  • CD71
  • CD71TRFR
  • OX26 monoclonal antibody
  • OX26 transferrin receptor
  • p90
  • T9
  • TfR (Transferrin R)
  • TfR (TransferrinR)
  • TfR
  • TFR1
  • TFRC
  • TR
  • transferrin receptor (p90, CD71)
  • transferrin receptor protein 1
  • TRFR

Background

OX26 TfR clone is useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. In comes in a variety of pre-conjugated fluorophores for histochemical and flow applications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TfR (Transferrin R) Antibody (NB200-585)(14)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: FLOW, IHC-Fr.


Filter By Application
FLOW
(3)
IHC-Fr
(1)
All Applications
Filter By Species
Rat
(4)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB200-585 Applications Species
Couty JP, Rampon C, Leveque M et al. PECAM-1 engagement counteracts ICAM-1-induced signaling in brain vascular endothelial cells. J Neurochem. [PMID: 17662049]
Gosk S, Vermehren C, Storm G et al. Targeting anti-transferrin receptor antibody (OX26) and OX26-conjugated liposomes to brain capillary endothelial cells using in situ perfusion. J Cereb Blood Flow Metab 24(11):1193-204. 2004 Nov. [PMID: 15545912]
Cox JH, Lamb JR, Bal V et al. The phenotypic and molecular characterization of Nb2 lymphoma cells activated with IL-2 and human growth hormone. Immunology. 1989 Jan 1 [PMID: 15493267] (FLOW, Rat) FLOW Rat
Moos T, Morgan EH. Restricted transport of anti-transferrin receptor antibody (OX26) through the blood-brain barrier in the rat. J Neurochem 79(1):119-29. 2001 Oct. [PMID: 11595764]
Crook K, Hunt SV. Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82. Dev Immunol. [PMID: 8924759] (FLOW, Rat) FLOW Rat
Broadwell RD, Baker-Cairns BJ, Friden PM et al. Transcytosis of protein through the mammalian cerebral epithelium and endothelium. III. Receptor-mediated transcytosis through the blood-brain barrier of blood-borne transferrin and antibody against the transferrin receptor. Exp Neurol. 1996 Nov 1 [PMID: 8912898]
Skarlatos S, Yoshikawa T, Pardridge WM. Transport of [125I]transferrin through the rat blood-brain barrier. Brain Res. 1995 Jun 19 [PMID: 7552351]
Paterson DJ, Williams AF. An intermediate cell in thymocyte differentiation that expresses CD8 but not CD4 antigen. J Exp Med. 1987 Nov 1 [PMID: 3500264] (FLOW, Rat) FLOW Rat
de Sousa M, Dynesius-Trentham R, Mota-Garcia F et al. Activation of rat synovium by iron. Arthritis Rheum. 1988 May 1 [PMID: 3377869]
De Sousa M, Breedvelt F, Dynesius-Trentham R et al. Iron, iron-binding proteins and immune system cells. Ann N Y Acad Sci. 1988 June 1 [PMID: 3291685]
Show All 14 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TfR (Transferrin R) Antibody (NB200-585). (Showing 1 - 2 of 2 FAQs).

  1. A customer would like to use this antibody to perform an functional assay. The customer would like to know whether this antibody will block the binding of transferrin to the receptor. On the other hand, would you mind to provide the immunogenic sequence of this antibody?
    • Unfortunately, this clone has not been mapped and the sequence of the epitope recognised is currently unknown. All that is currently known is that the clone binds an extracellular region not involved in transferrin binding.
  2. I was wondering if you have amino acid sequence information available for this monoclonal mouse anti-Rat TfR (Ox26) antibody.
    • The immunogen used to create this product is PHA activated rat lymphocytes (SwissProt: Q99376). We have not mapped the epitope for the product.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB200-585AF405
Alexa Fluor 488 Labeled NB200-585AF488
Alexa Fluor 647 Labeled NB200-585AF647
Alexa Fluor 700 Labeled NB200-585AF700
Allophycocyanin Labeled NB200-585APC
DyLight 350 Labeled NB200-585UV
DyLight 405 Labeled NB200-585V
DyLight 488 Labeled NB200-585G
DyLight 680 Labeled NB200-585FR
DyLight 755 Labeled NB200-585IR
PE Labeled NB200-585PE
PerCP Labeled NB200-585PCP

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Blogs on TfR (Transferrin R).

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

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Bioinformatics

Gene Symbol TFRC
Entrez
Uniprot