NHE3/SLC9A3 [p Ser552] Antibody (14D5)

Images

 
Western Blot: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Detection of NHE3 [p Ser552] in human kidney lysate.
Simple Western: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Simple Western lane view shows a specific band for NHE3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions ...read more
Immunofluorescence: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Mouse intestine, image courtesy of customer.

Product Details

Summary
Reactivity Hu, Mu, Rt, Op, Pm, RbSpecies Glossary
Applications WB, Simple Western, ELISA, ICC/IF, IHC, IP, IF
Clone
14D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2 mg/ml

NHE3/SLC9A3 [p Ser552] Antibody (14D5) Summary

Immunogen
Synthetic peptide made to a region surrounding the Serine 552 portion of rat NHE3. [UniProt# P26433]
Modification
p Ser552
Specificity
This antibody detects NHE3 posphorylated at Serine 552, and does not cross-react with the non-phosphopeptide.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SLC9A3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-5 ug/ml
  • Simple Western 1:1000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 1:50-1:1000
  • Immunoprecipitation 1:10-1:500
  • Immunofluorescence
Application Notes
This NHE3 [p Ser552] (14D5) antibody is useful in ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot, where a band can be seen at approx. 85 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB110-81529 in the following applications:

Publications
Read Publications using
NB110-81529 in the following applications:

  • 1 publication
  • 1 publication
  • 3 publications
  • IHC
    1 publication
  • IP
    4 publications
  • WB
    8 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
2 mg/ml
Purity
Protein G purified

Alternate Names for NHE3/SLC9A3 [p Ser552] Antibody (14D5)

  • isoform 3
  • MGC126718
  • NHE3
  • NHE3MGC126720
  • SLC9A3
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3
  • Solute carrier family 9 member 3

Background

Na+/H+ exchanger (NHE) family members are integral membrane proteins which play key role in catalyzing the extrusion of intracellular proton (H+) ions in exchange for extracellular sodium (Na+) ions for regulation of cellular pH as well as transepithelial ion/water transport. NHE3 or SLC9A3 is one of the best characterized NHEs that is a major proton extruding system driven by inward Na+ ion chemical gradient and is implicated in pH regulation to eliminate acids generated during metabolism or to tackle any potential adverse conditions in tissue microenvironment. NHE3 exists as a multi-pass membrane protein in cell membranes and is expressed most abundantly in colon and small intestine, followed by kidney and stomach, as well as in ventrolateral region of the medulla oblongata. In intestinal epithelial cells, it localizes to the ileal brush border and SGK1 mediated phosphorylation at Ser-661 has been shown to be associated with its enhanced cell membrane localization. NHE3 binds to SLC9A3R1 and SLC9A3R2, and interacts with CHP1, CHP2, SHANK2 PDZD3 and (interactions may decrease in response to elevated calcium ion levels). NHE3 is regulated by several hormones, neurotransmitters, and associated signaling systems such as cAMP, cGMP, and elevated intracellular calcium via mechanisms that are largely partially understood. NHE3 knock-out mice suffers from chronic diarrhea and altered salt as well as water homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-82576
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-76847
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB300-536
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-P, IP, IF
MAB25031
Species: Hu
Applications: WB, IHC, IP
NBP1-86376
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-2450
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP1-57622
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-38280
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-32020
Species: Hu
Applications: IHC, IHC-P
NBP1-30027
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84450
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-35213
Species: Hu
NB110-74682
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-16396
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84168
Species: Hu
Applications: IHC, IHC-P
AF1180
Species: Hu
Applications: WB, Simple Western, IHC, ICC
NBP2-20338
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB110-81529
Species: Hu, Mu, Rt, Op, Pm, Rb
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IP, IF

Publications for NHE3/SLC9A3 Antibody (NB110-81529)(8)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Opossum, Primate.

We have publications tested in 6 applications: ELISA, FLOW, ICC/IF, IHC, IP, WB.


Filter By Application
ELISA
(1)
FLOW
(1)
ICC/IF
(3)
IHC
(1)
IP
(4)
WB
(8)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
Rat
(3)
Opossum
(2)
Primate
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB110-81529 Applications Species
Ozkucur N, Song B, Bola S et al. NHE3 phosphorylation via PkCEta marks the polarity and orientation of directionally migrating cells. Cell. Mol. Life Sci. 2014 May 01 [PMID: 24788043] (WB, IP, ICC/IF, Human) WB, IP, ICC/IF Human
Perike S, Ozkucur N, Sharma P et al. Phospho-NHE3 forms membrane patches and interacts with beta-actin to sense and maintain constant direction during cell migration. Exp. Cell Res. 3/19/2014 [PMID: 24657527] (IP, WB, Human) IP, WB Human
Liu T, Jose PA. Gastrin Induces Sodium-Hydrogen Exchanger 3 Phosphorylation and mTOR Activation via a Phosphoinositide 3-Kinase-/Protein Kinase C-Dependent but AKT-Independent Pathway in Renal Proximal Tubule Cells Derived From a Normotensive Male Human. Endocrinology 2013 Feb [PMID: 23275470] (WB, Human) WB Human
Rieg T, Gerasimova M, Murray F et al. Natriuretic effect by exendin-4, but not the DPP-4 inhibitor alogliptin, is mediated via the GLP-1 receptor and preserved in obese type 2 diabetic mice Am J Physiol Renal Physiol 2012 Oct [PMID: 22832924] (WB, Mouse) WB Mouse
Ozkucur N, Perike S, Sharma P et al. Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directedness. BMC Cell Biol. 2011 Jan [PMID: 21255452] (FLOW, ICC/IF, IP, WB, Rat, Human) FLOW, ICC/IF, IP, WB Rat, Human
a DW, Steinmetz AG, Kocinsky HS. NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase. Am J Physiol Renal Physiol;298(3):F745-53. 2010 Mar. [PMID: 20015946] (WB, IP, Opossum, Primate) WB, IP Opossum, Primate
Kocinsky HS, Dynia DW, Wang T, Aronson PS. NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity. Am J Physiol Renal Physiol;293(1):F212-8. 2007 Jul. [PMID: 17409282] (WB, Rat) WB Rat
Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS. Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites. Am J Physiol Renal Physiol;289(2):F249-58. 2005 Aug. [PMID: 15687252] (WB, ELISA, ICC/IF, IHC, Primate, Opossum, Rat) WB, ELISA, ICC/IF, IHC Primate, Opossum, Rat

Review for NHE3/SLC9A3 Antibody (NB110-81529) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC-P.

Reviews using NB110-81529:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin NHE3/SLC9A3 NB110-81529
Enlarge
reviewed by:
Anjum Jafri
IHC-P Mouse 03/16/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Intestine
SpeciesMouse

Product General Protocols

View specific protocols for NHE3/SLC9A3 Antibody (NB110-81529): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NHE3/SLC9A3 Antibody (NB110-81529). (Showing 1 - 1 of 1 FAQs).

  1. What application was used to test on mouse species? Was it WB, ELISA, IHC-paraffin, IHC-frozen, ICC/IF, or etc?
    • We tested each lot in Western blot on human, mouse, and rat colon, small intestine and kidney samples. There were bands expressed around 25kDa and 50kDa, with weaker bands at approx. 80-90 kDa observed. This antibody is guaranteed to work in samples from mouse that are expected to express the protein. Since it is a phospho specific antibody the customer must use BSA to block and not milk.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-81529AF405
Alexa Fluor 488 Labeled NB110-81529AF488
Alexa Fluor 647 Labeled NB110-81529AF647
Alexa Fluor 700 Labeled NB110-81529AF700
Biotin Labeled NB110-81529B
DyLight 350 Labeled NB110-81529UV
DyLight 405 Labeled NB110-81529V
DyLight 488 Labeled NB110-81529G
DyLight 550 Labeled NB110-81529R
DyLight 650 Labeled NB110-81529C
DyLight 680 Labeled NB110-81529FR
DyLight 755 Labeled NB110-81529IR
FITC Labeled NB110-81529F
HRP Labeled NB110-81529H

Additional NHE3/SLC9A3 Products

Bioinformatics Tool for NHE3/SLC9A3 Antibody (NB110-81529)

Discover related pathways, diseases and genes to NHE3/SLC9A3 Antibody (NB110-81529). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NHE3/SLC9A3 Antibody (NB110-81529)

Discover more about diseases related to NHE3/SLC9A3 Antibody (NB110-81529).
 

Pathways for NHE3/SLC9A3 Antibody (NB110-81529)

View related products by pathway.

PTMs for NHE3/SLC9A3 Antibody (NB110-81529)

Learn more about PTMs related to NHE3/SLC9A3 Antibody (NB110-81529).
 

Research Areas for NHE3/SLC9A3 Antibody (NB110-81529)

Find related products by research area.

Blogs on NHE3/SLC9A3

There are no specific blogs for NHE3/SLC9A3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anjum Jafri
03/16/2017
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol SLC9A3
Entrez