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58K Golgi Protein Antibody (58K-9)

Images

 
Western Blot: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K golgi protein expression in rat liver tissue using NB600-412.
Immunocytochemistry/ Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - HeLa cells, cultured on cover slips, were fixed with 10% formalin for 10 minutes and then permeabilized for 5 minutes using 1X ...read more
Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412] - DMD muscle co-stained for Neu5Gc (green) with 58K Golgi, a Golgi marker, in red, and DAPI (blue). Arrow marks region of coincident staining (yellow) ...read more
Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K Golgi Protein in mouse kidney using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Assessing Golgi morphology observed apoptosis in mBECs using Mouse 58K Golgi Protein at a dilution of 1:100 overnight at 4 °C. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, ChHaSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
58K-9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

58K Golgi Protein Antibody (58K-9) Summary

Immunogen
58K Golgi Protein purified from rat liver [UniProt# O88618]
Localization
Cytoplasmic and Golgi Apparatus
Marker
Golgi Apparatus Marker
Predicted Species
Chinese Hamster (92%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FTCD
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:250
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 24505439)
  • Immunohistochemistry-Paraffin 1:100 - 1:200
  • Western Blot 1:2000 - 1:5000
Application Notes
In Western blot a band is observed at ~58 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 3.3
using
NB600-412 in the following applications:

Publications
Read Publications using
NB600-412 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for 58K Golgi Protein Antibody (58K-9)

  • formimidoyltransferase cyclodeaminase
  • formimidoyltransferase-cyclodeaminase
  • formiminotransferase cyclodeaminase
  • Formiminotransferase-cyclodeaminase
  • human formiminotransferase cyclodeaminase, EC 4.3.1.410formiminotransferase-cyclodeaminase
  • LCHC1

Background

The 58K Golgi protein is one of the most commonly used Golgi markers and has been proposed to implicate in linking Golgi to microtubules and it peripherally associates with cytosolic surface of Golgi membranes. This protein shows extensive overall sequence identity with porcine FTCD and has been suggested to represent rat FTCD (formiminotransferase-cyclodeaminase), a metabolic enzyme involved in the conversion of histidine to glutamic acid. FTCD's formiminotransferase activity facilitate transfer of a a formimino group from N-formimino-L-glutamic acid to tetrahydrofolate to generate glutamic acid and 5-formiminotetrahydrofolate, and the cyclodeaminase activity then converts the 5-formiminotetrahydrofolate to 5,10-methenyl tetrahydrofolate and ammonia. FTCD appears to be a dynamic component of Golgi and cycles between Golgi and earlier compartments of the secretory pathway. It also binds and promotes bundling of vimentin filaments originating from the Golgi, and mutations in this gene encoding for 58K Golgi Protein i.e. FTCD are associated with glutamate formiminotransferase deficiency (FIGLU-URIA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 58K Golgi Protein Antibody (NB600-412)(12)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 3 applications: ICC/IF, IHC-Fr, WB.


Filter By Application
ICC/IF
(8)
IHC-Fr
(1)
WB
(4)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
Rat
(5)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB600-412 Applications Species
Martin PT, Golden B, Okerblom J et al. A Comparative Study of N-glycolylneuraminic Acid (Neu5Gc) and Cytotoxic T Cell (CT) Carbohydrate Expression in Normal and Dystrophin-Deficient Dog and Human Skeletal Muscle. PLoS ONE 2014-02-07 [PMID: 24505439] (IHC-Fr, Human) IHC-Fr Human
Murphy AJ, Bijl N, Yvan-Charvet L et al. Cholesterol efflux in megakaryocyte progenitors suppresses platelet production and thrombocytosis. Nat Med 2013-05-01 [PMID: 23584088] (ICC/IF, Mouse) ICC/IF Mouse
Nakonechnaya AO, Jefferson HS, Chen X, Shewchuk BM. Differential effects of exogenous and autocrine growth hormone on LNCaP prostate cancer cell proliferation and survival J Cell Biochem 2012-12-13 [PMID: 23238889] (ICC/IF, Human) ICC/IF Human
Azad AK, Torrelles JB, Schlesinger LS. Mutation in the DC-SIGN cytoplasmic triacidic cluster motif markedly attenuates receptor activity for phagocytosis and endocytosis of mannose-containing ligands by human myeloid cells. J Leukoc Biol;84(6):1594-603. 2008-12-01 [PMID: 18772280]
Young KG, Pinheiro B, Kothary R. A Bpag1 isoform involved in cytoskeletal organization surrounding the nucleus. Exp Cell Res;312(2):121-34. 2006-01-15 [PMID: 16289082] (ICC/IF, Rat, Mouse) ICC/IF Rat, Mouse
Gao YS, Alvarez C, Nelson DS, Sztul E. Molecular cloning, characterization, and dynamics of rat formiminotransferase cyclodeaminase, a Golgi-associated 58-kDa protein. J Biol Chem;273(50):33825-34. 1998-12-11 [PMID: 9837973] (ICC/IF, Rat) ICC/IF Rat
Bashour AM, Bloom GS. 58K, a microtubule-binding Golgi protein, is a formiminotransferase cyclodeaminase. J Biol Chem;273(31):19612-7. 1998-07-31 [PMID: 9677387] (WB, Rat) WB Rat
Hennig D, Scales SJ, Moreau A, Murley LL, De Mey J, Kreis TE. A formiminotransferase cyclodeaminase isoform is localized to the Golgi complex and can mediate interaction of trans-Golgi network-derived vesicles with microtubules. J Biol Chem;273(31):19602-11. 1998-07-31 [PMID: 9677386] (WB, ICC/IF, Human, Chicken) WB, ICC/IF Human, Chicken
Lippincott-Schwartz J, Cole NB, Marotta A, Conrad PA, Bloom GS. Kinesin is the motor for microtubule-mediated Golgi-to-ER membrane traffic. J Cell Biol;128(3):293-306. 1995-02-01 [PMID: 7844144] (ICC/IF, Rat, Human) ICC/IF Rat, Human
Bloom GS, Brashear TA. A novel 58-kDa protein associates with the Golgi apparatus and microtubules. J Biol Chem 1989-09-25 [PMID: 2777777] (WB, ICC/IF, Rat) WB, ICC/IF Rat
Show All 12 Publications.

Reviews for 58K Golgi Protein Antibody (NB600-412) (3) 3.33

Average Rating: 3.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NB600-412:
Filter by Applications
ICC
(3)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry 58K Golgi Protein NB600-412
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2
reviewed by:
Verified Customer
ICC Mouse 03/22/2023
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse Brain Endothelial Cells
SpeciesMouse
LotA-8

Comments

Comments***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
Immunocytochemistry 58K Golgi Protein NB600-412
Enlarge
4
reviewed by:
Ali Almousawi
ICC Mouse 03/13/2023
View

Summary

ApplicationImmunocytochemistry
Sample TestedCultured Brain Endothelial cells,Cultured Mouse brain endothelial cells,Mouse Brain Endothelial cells (mBECs)
SpeciesMouse
LotA-8
Immunocytochemistry 58K Golgi Protein NB600-412
Enlarge
4
reviewed by:
Verified Customer
ICC Mouse 11/19/2018
View

Summary

ApplicationImmunocytochemistry
Sample Testedmouse vascular smooth muscle cell
SpeciesMouse
LotA2

Product General Protocols

View specific protocols for 58K Golgi Protein Antibody (NB600-412): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 58K Golgi Protein Antibody (NB600-412) (0)

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Recent Reviews

3.3
5
0
4
2
3
0
2
1
1
0

Verified Customer
03/22/2023
Application: ICC
Species: Mouse

Ali Almousawi
03/13/2023
Application: ICC
Species: Mouse

Verified Customer
11/19/2018
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol FTCD
Entrez
Uniprot