Western Blot: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K golgi protein expression in rat liver tissue using NB600-412.
Immunocytochemistry/ Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - HeLa cells, cultured on cover slips, were fixed with 10% formalin for 10 minutes and then permeabilized for 5 minutes using 1X ...read more
Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K Golgi Protein in mouse kidney using DAB with hematoxylin counterstain.
This 58K Golgi Protein antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, and Immunohistochemistry paraffin-embedded sections. In Western blot a band is observed at ~58 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24505439) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
58 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Rat's 58K Golgi Protein sequence is 92% similar with Chinese hamster (Cricetulus griseus)
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1% Sodium Azide
Alternate Names for 58K Golgi Protein Antibody (58K-9)
human formiminotransferase cyclodeaminase, EC 22.214.171.1240formiminotransferase-cyclodeaminase
The 58K Golgi protein is one of the most commonly used Golgi markers and has been proposed to implicate in linking Golgi to microtubules and it peripherally associates with cytosolic surface of Golgi membranes. This protein shows extensive overall sequence identity with porcine FTCD and has been suggested to represent rat FTCD (formiminotransferase-cyclodeaminase), a metabolic enzyme involved in the conversion of histidine to glutamic acid. FTCD's formiminotransferase activity facilitate transfer of a a formimino group from N-formimino-L-glutamic acid to tetrahydrofolate to generate glutamic acid and 5-formiminotetrahydrofolate, and the cyclodeaminase activity then converts the 5-formiminotetrahydrofolate to 5,10-methenyl tetrahydrofolate and ammonia. FTCD appears to be a dynamic component of Golgi and cycles between Golgi and earlier compartments of the secretory pathway. It also binds and promotes bundling of vimentin filaments originating from the Golgi, and mutations in this gene encoding for 58K Golgi Protein i.e. FTCD are associated with glutamate formiminotransferase deficiency (FIGLU-URIA).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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