58K Golgi Protein Antibody (58K-9)

Images

 
Western Blot: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K golgi protein expression in rat liver tissue using NB600-412.
Immunocytochemistry/ Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - HeLa cells, cultured on cover slips, were fixed with 10% formalin for 10 minutes and then permeabilized for 5 minutes using 1X ...read more
Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K Golgi Protein in mouse kidney using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, ChHaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
58K-9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

58K Golgi Protein Antibody (58K-9) Summary

Immunogen
58K Golgi Protein purified from rat liver [UniProt# O88618]
Localization
Cytoplasmic and Golgi Apparatus
Marker
Golgi Apparatus Marker
Predicted Species
Chinese Hamster (92%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FTCD
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:200
Application Notes
This 58K Golgi Protein antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, and Immunohistochemistry paraffin-embedded sections. In Western blot a band is observed at ~58 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24505439) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB600-412 in the following applications:

Reactivity Notes

Rat's 58K Golgi Protein sequence is 92% similar with Chinese hamster (Cricetulus griseus)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for 58K Golgi Protein Antibody (58K-9)

  • formimidoyltransferase cyclodeaminase
  • formimidoyltransferase-cyclodeaminase
  • formiminotransferase cyclodeaminase
  • Formiminotransferase-cyclodeaminase
  • human formiminotransferase cyclodeaminase, EC 4.3.1.410formiminotransferase-cyclodeaminase
  • LCHC1

Background

The 58K Golgi protein is one of the most commonly used Golgi markers and has been proposed to implicate in linking Golgi to microtubules and it peripherally associates with cytosolic surface of Golgi membranes. This protein shows extensive overall sequence identity with porcine FTCD and has been suggested to represent rat FTCD (formiminotransferase-cyclodeaminase), a metabolic enzyme involved in the conversion of histidine to glutamic acid. FTCD's formiminotransferase activity facilitate transfer of a a formimino group from N-formimino-L-glutamic acid to tetrahydrofolate to generate glutamic acid and 5-formiminotetrahydrofolate, and the cyclodeaminase activity then converts the 5-formiminotetrahydrofolate to 5,10-methenyl tetrahydrofolate and ammonia. FTCD appears to be a dynamic component of Golgi and cycles between Golgi and earlier compartments of the secretory pathway. It also binds and promotes bundling of vimentin filaments originating from the Golgi, and mutations in this gene encoding for 58K Golgi Protein i.e. FTCD are associated with glutamate formiminotransferase deficiency (FIGLU-URIA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Bv, Ca, Ch, ChHa
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for 58K Golgi Protein Antibody (NB600-412)(10)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 3 applications: ICC/IF, IHC-Fr, WB.


Filter By Application
ICC/IF
(7)
IHC-Fr
(1)
WB
(3)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
Rat
(5)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB600-412 Applications Species
Martin PT, Golden B, Okerblom J et al. A Comparative Study of N-glycolylneuraminic Acid (Neu5Gc) and Cytotoxic T Cell (CT) Carbohydrate Expression in Normal and Dystrophin-Deficient Dog and Human Skeletal Muscle. PLoS ONE 2014 Feb 7 [PMID: 24505439] (IHC-Fr, Human) IHC-Fr Human
Murphy AJ, Bijl N, Yvan-Charvet L et al. Cholesterol efflux in megakaryocyte progenitors suppresses platelet production and thrombocytosis. Nat Med 2013 May [PMID: 23584088] (ICC/IF, Mouse) ICC/IF Mouse
Nakonechnaya AO, Jefferson HS, Chen X, Shewchuk BM. Differential effects of exogenous and autocrine growth hormone on LNCaP prostate cancer cell proliferation and survival J Cell Biochem 2012 Dec 13 [PMID: 23238889] (ICC/IF, Human) ICC/IF Human
Azad AK, Torrelles JB, Schlesinger LS. Mutation in the DC-SIGN cytoplasmic triacidic cluster motif markedly attenuates receptor activity for phagocytosis and endocytosis of mannose-containing ligands by human myeloid cells. J Leukoc Biol;84(6):1594-603. 2008 Dec. [PMID: 18772280]
Young KG, Pinheiro B, Kothary R. A Bpag1 isoform involved in cytoskeletal organization surrounding the nucleus. Exp Cell Res;312(2):121-34. 2006 Jan 15. [PMID: 16289082] (ICC/IF, Mouse, Rat) ICC/IF Mouse, Rat
Gao YS, Alvarez C, Nelson DS, Sztul E. Molecular cloning, characterization, and dynamics of rat formiminotransferase cyclodeaminase, a Golgi-associated 58-kDa protein. J Biol Chem;273(50):33825-34. 1998 Dec 11. [PMID: 9837973] (ICC/IF, Rat) ICC/IF Rat
Bashour AM, Bloom GS. 58K, a microtubule-binding Golgi protein, is a formiminotransferase cyclodeaminase. J Biol Chem;273(31):19612-7. 1998 Jul 31. [PMID: 9677387] (WB, Rat) WB Rat
Hennig D, Scales SJ, Moreau A, Murley LL, De Mey J, Kreis TE. A formiminotransferase cyclodeaminase isoform is localized to the Golgi complex and can mediate interaction of trans-Golgi network-derived vesicles with microtubules. J Biol Chem;273(31):19602-11. 1998 Jul 31. [PMID: 9677386] (WB, ICC/IF, Human, Chicken) WB, ICC/IF Human, Chicken
Lippincott-Schwartz J, Cole NB, Marotta A, Conrad PA, Bloom GS. Kinesin is the motor for microtubule-mediated Golgi-to-ER membrane traffic. J Cell Biol;128(3):293-306. 1995 Feb. [PMID: 7844144] (ICC/IF, Human, Rat) ICC/IF Human, Rat
Bloom GS, Brashear TA. A novel 58-kDa protein associates with the Golgi apparatus and microtubules. J Biol Chem;264(27):16083-92. 1989 Sep 25. [PMID: 2777777] (WB, ICC/IF, Rat) WB, ICC/IF Rat

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Bioinformatics

Gene Symbol FTCD
Entrez
Uniprot