58K Golgi Protein Antibody (58K-9)

Western Blot: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K golgi protein expression in rat liver tissue using NB600-412.

Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - HeLa cells, cultured on cover slips, were fixed with 10% formalin for 10 minutes and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were then incubated with 1:200 dilution of anti-58K Golgi Protein antibody (clone 58K-9) for overnight at 4C and detected with an anti-mouse Dylight 488 (Green) secondary at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at 1:500 dilution. Nuclei were counterstained with DAPI solution (Blue) [NBP2-31156]. Cells were imaged using a 40X objective. Antibody clone 58K-9 generated a specific signal in the Golgi apparatus of the cells.

Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412] - DMD muscle co-stained for Neu5Gc (green) with 58K Golgi, a Golgi marker, in red, and DAPI (blue). Arrow marks region of coincident staining (yellow) for Neu5Gc and 58K Golgi. Bar is 50 um. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0088226) licensed under a CC-BY license.

Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K Golgi Protein in mouse kidney using DAB with hematoxylin counterstain.

Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Assessing Golgi morphology observed apoptosis in mBECs using Mouse 58K Golgi Protein at a dilution of 1:100 overnight at 4 °C. This image was taken on a Zeiss LSM 710 Confocal Microscope (META) with Zeiss Plan Apo 63x/1.40 oil. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, ChHa
• Applications: WB, ICC/IF, IHC
• Clone: 58K-9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

58K Golgi Protein Antibody (58K-9) Summary

• Immunogen: 58K Golgi Protein purified from rat liver [UniProt# O88618]
• Localization: Cytoplasmic and Golgi Apparatus
• Marker: Golgi Apparatus Marker
• Predicted Species: Chinese Hamster (92%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FTCD
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:250
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 24505439)
  • Immunohistochemistry-Paraffin 1:100 - 1:200
  • Western Blot 1:2000 - 1:5000
• Application Notes: In Western blot a band is observed at ~58 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for 58K Golgi Protein Antibody (58K-9)

• formimidoyltransferase cyclodeaminase
• formimidoyltransferase-cyclodeaminase
• formiminotransferase cyclodeaminase
• Formiminotransferase-cyclodeaminase
• human formiminotransferase cyclodeaminase, EC 4.3.1.410formiminotransferase-cyclodeaminase
• LCHC1

Background

The 58K Golgi protein is one of the most commonly used Golgi markers and has been proposed to implicate in linking Golgi to microtubules and it peripherally associates with cytosolic surface of Golgi membranes. This protein shows extensive overall sequence identity with porcine FTCD and has been suggested to represent rat FTCD (formiminotransferase-cyclodeaminase), a metabolic enzyme involved in the conversion of histidine to glutamic acid. FTCD's formiminotransferase activity facilitate transfer of a a formimino group from N-formimino-L-glutamic acid to tetrahydrofolate to generate glutamic acid and 5-formiminotetrahydrofolate, and the cyclodeaminase activity then converts the 5-formiminotetrahydrofolate to 5,10-methenyl tetrahydrofolate and ammonia. FTCD appears to be a dynamic component of Golgi and cycles between Golgi and earlier compartments of the secretory pathway. It also binds and promotes bundling of vimentin filaments originating from the Golgi, and mutations in this gene encoding for 58K Golgi Protein i.e. FTCD are associated with glutamate formiminotransferase deficiency (FIGLU-URIA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 58K Golgi Protein Antibody (NB600-412)(12)

Publications using NB600-412

• Martin PT, Golden B, Okerblom J et al. A Comparative Study of N-glycolylneuraminic Acid (Neu5Gc) and Cytotoxic T Cell (CT) Carbohydrate Expression in Normal and Dystrophin-Deficient Dog and Human Skeletal Muscle. PLoS ONE 2014-02-07 [PMID: 24505439] (IHC-Fr, Human)
• Murphy AJ, Bijl N, Yvan-Charvet L et al. Cholesterol efflux in megakaryocyte progenitors suppresses platelet production and thrombocytosis. Nat Med 2013-05-01 [PMID: 23584088] (ICC/IF, Mouse)
• Nakonechnaya AO, Jefferson HS, Chen X, Shewchuk BM. Differential effects of exogenous and autocrine growth hormone on LNCaP prostate cancer cell proliferation and survival J Cell Biochem 2012-12-13 [PMID: 23238889] (ICC/IF, Human)
• Azad AK, Torrelles JB, Schlesinger LS. Mutation in the DC-SIGN cytoplasmic triacidic cluster motif markedly attenuates receptor activity for phagocytosis and endocytosis of mannose-containing ligands by human myeloid cells. J Leukoc Biol;84(6):1594-603. 2008-12-01 [PMID: 18772280]
• Young KG, Pinheiro B, Kothary R. A Bpag1 isoform involved in cytoskeletal organization surrounding the nucleus. Exp Cell Res;312(2):121-34. 2006-01-15 [PMID: 16289082] (ICC/IF, Rat, Mouse)
• Gao YS, Alvarez C, Nelson DS, Sztul E. Molecular cloning, characterization, and dynamics of rat formiminotransferase cyclodeaminase, a Golgi-associated 58-kDa protein. J Biol Chem;273(50):33825-34. 1998-12-11 [PMID: 9837973] (ICC/IF, Rat)
• Bashour AM, Bloom GS. 58K, a microtubule-binding Golgi protein, is a formiminotransferase cyclodeaminase. J Biol Chem;273(31):19612-7. 1998-07-31 [PMID: 9677387] (WB, Rat)
• Hennig D, Scales SJ, Moreau A, Murley LL, De Mey J, Kreis TE. A formiminotransferase cyclodeaminase isoform is localized to the Golgi complex and can mediate interaction of trans-Golgi network-derived vesicles with microtubules. J Biol Chem;273(31):19602-11. 1998-07-31 [PMID: 9677386] (WB, ICC/IF, Human, Chicken)
• Lippincott-Schwartz J, Cole NB, Marotta A, Conrad PA, Bloom GS. Kinesin is the motor for microtubule-mediated Golgi-to-ER membrane traffic. J Cell Biol;128(3):293-306. 1995-02-01 [PMID: 7844144] (ICC/IF, Rat, Human)
• Bloom GS, Brashear TA. A novel 58-kDa protein associates with the Golgi apparatus and microtubules. J Biol Chem 1989-09-25 [PMID: 2777777] (WB, ICC/IF, Rat)
• Zhou A, Dong X, Tang B TNK2/ACK1 Strengthen Influenza A Virus Infection by Blocking Viral Matrix 2 Protein(M2) into Lysosome to Degradation Research Square 2021-01-29 (ICC/IF)
• Bharat V, Hsieh CH, Wang X Mitochondrial Defects in Fibroblasts of Pathogenic MAPT Patients Frontiers in cell and developmental biology 2021-11-03 [PMID: 34805172] (WB, Human)

Reviews for 58K Golgi Protein Antibody (NB600-412) (3)

Average Rating: 3.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer ICC Mouse 03/22/2023

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse Brain Endothelial Cells
• Species: Mouse
• Lot: A-8

Comments

• Comments: ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

reviewed by: Verified Customer ICC Mouse 03/13/2023

Summary

• Application: Immunocytochemistry
• Sample Tested: Cultured Brain Endothelial cells,Cultured Mouse brain endothelial cells,Mouse Brain Endothelial cells (mBECs)
• Species: Mouse
• Lot: A-8

reviewed by: Shinobu Miyazaki ICC Mouse 11/19/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: mouse vascular smooth muscle cell
• Species: Mouse
• Lot: A2

Bioinformatics

• Gene Symbol: FTCD
• Entrez:
  • 10841(Human)
  • 14317(Mouse)
  • 89833(Rat)
• Uniprot:
  • O95954(Human)
  • Q91XD4(Mouse)
  • O88618(Rat)