Western Blot: GLI-1 Antibody [NBP1-78259] - Analysis of GLI-1 on partial recombinant GLI-1 protein (molecular weight of partial recombinant protein is 37 kDa).
Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody [NBP1-78259] - GLI1 antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunohistochemistry: GLI-1 Antibody [NBP1-78259] - Analysis of GLI-1 in mouse brain using DAB with hematoxylin counterstain.
A synthetic peptide made to an internal portion of the human Gli1 protein (between residues 150-200) [UniProt P08151]
Cytoplasm. Nucleus. Note: Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization.
Immunogen affinity purified
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This Gli1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, and Western Blot. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25799059). Use in In-situ Hybridization reported in scientific literature (PMID: 24506883).
Read Publications using NBP1-78259 in the following applications:
GLI-1 is a transcription factor involved in development, patterning and the regulation of cell fate-determination. A down-stream effector of the Hedgehog (HH) signaling pathway, GLI proteins work with other transcriptional activators to up-regulate the expression of GLI-response genes. In addition to its role in embryogenesis, the aberrant activation of the HH/GLI pathway has been identified in numerous forms of cancer, implicating the GLI family of proteins as potential therapeutic targets. In the absence of a stimulus, GLI-1 is anchored to the cytoplasm via Cos-2, which is then released and translocates to the nucleus upon activation of the HH pathway. In the nucleus, GLI-1 binds DNA via a highly conserved C2-H2 zinc finger domain and acts exclusively as an activator increasing transcription of GLI1, PTCH1, PTCH2, HHIP1, MYCN, CCND1, CCND2, BCL2, CFLAR, FOXF1, FOXL1, PRDM1 (BLIMP1), JAG2, GREM1, and Follistatin.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for GLI-1 Antibody (NBP1-78259). (Showing 1 - 1 of 1 FAQs).
What protocols did you use to stain mouse tissues (IHC-P) with NBP1-78259? For antigen retrieval, did you use boiling in citric buffer? What did you use for blocking? What do you dilute the antibodies in and what is the dilution when you are incubating with NBP1-78259?
Please see this link to our specific protocol for using this antibody in IHC. I think this should provide you with the answers you needed.
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