GLI-1 Antibody

Images

 
Western Blot: GLI-1 Antibody [NBP1-78259] - Analysis of GLI-1 on partial recombinant GLI-1 protein (molecular weight of partial recombinant protein is 37 kDa).
Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody [NBP1-78259] - GLI1 antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunohistochemistry: GLI-1 Antibody [NBP1-78259] - Analysis of GLI-1 in mouse brain using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, ISH
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.08 mg/ml

GLI-1 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human Gli1 protein (between residues 150-200) [UniProt P08151]
Localization
Cytoplasm. Nucleus. Note: Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:400
  • In-situ Hybridization
Application Notes
This Gli1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, and Western Blot. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25799059). Use in In-situ Hybridization reported in scientific literature (PMID: 24506883).
Publications
Read Publications using
NBP1-78259 in the following applications:

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.08 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GLI-1 Antibody

  • GLI family zinc finger 1
  • GLI1
  • GLI-1
  • glioma-associated oncogene family zinc finger 1
  • glioma-associated oncogene homolog 1 (zinc finger protein)
  • Glioma-associated oncogene
  • GLIzinc finger protein GLI1
  • Oncogene GLI

Background

GLI-1 is a transcription factor involved in development, patterning and the regulation of cell fate-determination. A down-stream effector of the Hedgehog (HH) signaling pathway, GLI proteins work with other transcriptional activators to up-regulate the expression of GLI-response genes. In addition to its role in embryogenesis, the aberrant activation of the HH/GLI pathway has been identified in numerous forms of cancer, implicating the GLI family of proteins as potential therapeutic targets. In the absence of a stimulus, GLI-1 is anchored to the cytoplasm via Cos-2, which is then released and translocates to the nucleus upon activation of the HH pathway. In the nucleus, GLI-1 binds DNA via a highly conserved C2-H2 zinc finger domain and acts exclusively as an activator increasing transcription of GLI1, PTCH1, PTCH2, HHIP1, MYCN, CCND1, CCND2, BCL2, CFLAR, FOXF1, FOXL1, PRDM1 (BLIMP1), JAG2, GREM1, and Follistatin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GLI-1 Antibody (NBP1-78259)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, ISH, WB.


Filter By Application
ICC/IF
(1)
IHC
(4)
IHC-Fr
(1)
IHC-P
(1)
ISH
(1)
WB
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(6)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-78259 Applications Species
Yu M, Ma L, Yuan Y, et al. Cranial Suture Regeneration Mitigates Skull and Neurocognitive Defects in Craniosynostosis Cell Jan 7 2021 [PMID: 33417861] (ISH, Mouse) ISH Mouse
Durham E, Howie RN, Larson N et al. Pharmacological exposures may precipitate craniosynostosis through targeted stem cell depletion Stem Cell Res Aug 6 2019 [PMID: 31415959] (IHC-P, Mouse) IHC-P Mouse
Chiang A, Tan CZ, Kuonen F et al. Genetic mutations underlying phenotypic plasticity in basosquamous carcinoma J. Invest. Dermatol. Jun 14 2019 [PMID: 31207229] (Human) Human
Durham E L, Howie R N et al. Involvement of Calvarial Stem Cells in Healing: A Regional Analysis of Large Cranial Defects. Wound Repair Regen Jul 28 2018 [PMID: 30054956] (IHC, Mouse) IHC Mouse
Liu Y, Feng J, Li J et al. An Nfic-hedgehog signaling cascade regulates tooth root development. Development. 2015 Aug 20 [PMID: 26293299] (IHC, Mouse) IHC Mouse
Zhao H, Feng J, Ho TV et al. The suture provides a niche for mesenchymal stem cells of craniofacial bones. Nat. Cell Biol. 2015 Apr 01 [PMID: 25799059] (IHC-Fr, Mouse) IHC-Fr Mouse
Zhao Hu, Feng Jifan, Seidel Kerstin et al. Secretion of shh by a neurovascular bundle niche supports mesenchymal stem cell homeostasis in the adult mouse incisor. Cell Stem Cell. 2014 Feb 06 [PMID: 24506883]
Jalili A, Mertz KD, Romanov J et al. NVP-LDE225, a Potent and Selective SMOOTHENED Antagonist Reduces Melanoma Growth In Vitro and In Vivo. PLoS One 2013 Jul 30 [PMID: 23935925] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Zhang L, Zhao J, Dong J et al. GSK3 beta RephosphorylationRescues ALPLDeficiency Induced-impairment of Odontoblastic Differentiation of DPSCs Research Square Dec 10 2020 (WB, Human) WB Human
Ren Y, Su S, Liu X et al. Microbiota-derived short-chain fatty acids promote BMP signaling by inhibiting histone deacetylation and contribute to dentinogenic differentiation in murine incisor regeneration Stem Cells Dev. Jul 21 2020 [PMID: 32689895]
Show All 11 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GLI-1 Antibody (NBP1-78259). (Showing 1 - 1 of 1 FAQs).

  1. What protocols did you use to stain mouse tissues (IHC-P) with NBP1-78259? For antigen retrieval, did you use boiling in citric buffer? What did you use for blocking? What do you dilute the antibodies in and what is the dilution when you are incubating with NBP1-78259?
    • Please see this link to our specific protocol for using this antibody in IHC. I think this should provide you with the answers you needed.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-78259AF350
Alexa Fluor 405 NBP1-78259AF405
Alexa Fluor 488 NBP1-78259AF488
Alexa Fluor 532 NBP1-78259AF532
Alexa Fluor 594 NBP1-78259AF594
Alexa Fluor 647 NBP1-78259AF647
Alexa Fluor 700 NBP1-78259AF700
Alexa Fluor 750 NBP1-78259AF750
Biotin NBP1-78259B
DyLight 350 NBP1-78259UV
DyLight 405 NBP1-78259V
DyLight 488 NBP1-78259G
DyLight 550 NBP1-78259R
DyLight 650 NBP1-78259C
DyLight 680 NBP1-78259FR
DyLight 755 NBP1-78259IR
FITC NBP1-78259F
HRP NBP1-78259H
Janelia Fluor 549 NBP1-78259JF549
Janelia Fluor 646 NBP1-78259JF646

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Bioinformatics

Gene Symbol GLI1
Entrez
Uniprot