D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free

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Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - WB analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (NB100-56725, Clone DX2). Harvested ...read more
Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) ...read more
Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Intracellular analysis of cleaved D4-GDI in 10^6 untreated (left) and 3 hour staurosporine-treated (right) Jurkat cells using ...read more
Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Western blot analysis of D4GDI in Jurkat cells using NB100-56556 (IMG-396A) at 2 ug/ml and NB100-56725 (IMG-160A) at 0.1 ug/ml. Cells ...read more
Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by ...read more
Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Analysis using the FITC conjugate of NB100-56725. Staining of cleaved D4-GDI in 10^6 camptothecin-treated (red) and untreated ...read more
Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Analysis using the FITC conjugate of NB100-56725. Staining of cleaved D4-GDI in untreated (left) and staurosporine-treated ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, Flow-IC, IHC, IHC-P, IP
Clone
97A1015
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free Summary

Immunogen
A synthetic peptide corresponding to the region of the Fas-induced cleavage site of human D4-GDI/RhoGDI2 was used as the immunogen.
Specificity
In anti-Fas treated Jurkat, an approx. 23 kDa band should be observed.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ARHGDIB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular) 0.5-1 ug/10^6 cells
  • Flow Cytometry 0.5-1 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1-2 ug/ml
  • Western Blot 2 ug/ml
Control
D4-GDI/RhoGDI2 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-56725 in the following applications:

Publications
Read Publications using
NB100-56725 in the following applications:

  • 1 publication
  • WB
    3 publications

Reactivity Notes

Cross reacts with Human and Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) - BSA Free

  • ARHGDIB Rho GDP dissociation inhibitor (GDI) beta
  • D4
  • GDIA2
  • GDID4
  • LYGDI
  • Ly-GDI
  • RAP1GN1
  • RhoGDI2

Background

D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for D4-GDI/RhoGDI2 Antibody (NB100-56725)(16)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(3)
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Mouse
(2)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-56725 Applications Species
Nishtala K, Phong TQ, Steil L et al. Proteomic analyses of age related changes in A.BY/SnJ mouse hearts. Proteome Sci Proteome Sci [PMID: 23816347] (IHC-P, Mouse) IHC-P Mouse
Okamoto M, Koga S, Tatsuka M. Differential regulation of caspase-9 by ionizing radiation- and UV-induced apoptotic pathways in thymic cells. Mutat Res. 2010 Jun 1 [PMID: 20346366] (WB, Mouse)

Details:
D4-GDI (IMG-160A). WB: Mouse lymphoma 3SB cell line subject to either ionizing radiation or UV light, Fig 2D.
WB Mouse
Hu LD, Zou HF, Zhan SX, Cao KM. Biphasic expression of RhoGDI2 in the progression of breast cancer and its negative relation with lymph node metastasis. Oncol Rep. 2007 Jun [PMID: 17487395] (WB)

Details:
IHC (P): Fig 1 and Table I (human breast tumor and normal adjacent tissues (Fig 1 and Table 1); WB: Fig 2A (human breast cancer cell lines).
WB
Jenkins CE, Swiatoniowski A, Power MR, Lin TJ. Pseudomonas aeruginosa-induced human mast cell apoptosis is associated with up-regulation of endogenous Bcl-xS and down-regulation of Bcl-xL. J Immunol. 2006 Dec 1 [PMID: 17114473]
Wang Z, Zhang X, Shen P et al. A variant of estrogen receptor-{alpha}, hER-{alpha}36: transduction of estrogen- and antiestrogen-dependent membrane-initiated mitogenic signaling. Proc Natl Acad Sci U S A. 2006 Jun 13 [PMID: 16754886] (WB)

Details:
WB: HEk293 whole cell lysates (W), plasma membrane (M), cytosolic (C), and nuclear (N) fractions, Fig 3.
WB
Jenkins CE, Swiatoniowski A, Issekutz AC, Lin TJ. Pseudomonas aeruginosa exotoxin A induces human mast cell apoptosis by a caspase-8 and -3-dependent mechanism. J Biol Chem. 2004 Aug 27 [PMID: 15205454]
Liu CY, Lee CF, Hong CH, Wei YH. Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis. Ann N Y Acad Sci. 2004 Apr [PMID: 15126291]
Ko CH, Shen SC, Chen YC. Hydroxylation at C4' or C6 is essential for apoptosis-inducing activity of flavanone through activation of the caspase-3 cascade and production of reactive oxygen species. Free Radic Biol Med. 2004 Apr 1 [PMID: 15019974]
Chen YC, Shen SC, Lin HY. Rutinoside at C7 attenuates the apoptosis-inducing activity of flavonoids. Biochem Pharmacol. 2003 Oct 1 [PMID: 14505793]
Shen SC, Chen YC, Hsu FL, Lee WR. Differential apoptosis-inducing effect of quercetin and its glycosides in human promyeloleukemic HL-60 cells by alternative activation of the caspase 3 cascade. J Cell Biochem. 2003 Aug 1 [PMID: 12874837]
Show All 16 Publications.

Review for D4-GDI/RhoGDI2 Antibody (NB100-56725) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56725:
Filter by Applications
Western Blot
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot D4-GDI/RhoGDI2 NB100-56725
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4
reviewed by:
Qing Xu
Western Blot Human 07/18/2018
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Summary

ApplicationWestern Blot
Sample TestedHT-29 human colon adenocarcinoma cell line
SpeciesHuman
LotAB021110A-04

Comments

CommentsHT-29 cells were treated with etoposide (100 ug/ml) for indicated time points. Total cell lysates were subjected to western blot. PVDF membrane were probed with 2 um/ml D4-GDI/RhoGDI2 (cleavage specific) Antibody (NB100-56725). A specific band was detected for cleaved RhoGDI2 at approximately 23 kDa. This experiment was conducted under reducing conditions.

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Control Lysate(s)

Secondary Antibodies

 

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Blogs on D4-GDI/RhoGDI2.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
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2
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1
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Qing Xu
07/18/2018
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol ARHGDIB