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![Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.](http://images.novusbio.com/fullsize/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0005.jpg "Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.")
![<b>Biological Strategies Validation. </b>Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Western Blot: [NBP2-49688] - 10 ug of Chloroquine treated (+) and untreated (-) Neuro2A lysates in 1x Laemmli sample buffer (NBP2-49688) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml monoclonal anti-LC3 (NBP2--46892) and detected with an anti-rabbit secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0004.jpg)
![Western Blot: TLR2 Cell Lysate [NBP2-30165] - 15 ul of mouse TLR2 transfected cell lysate tested with IMG-526 (A) and IMG-662 (B).](http://images.novusbio.com/fullsize/TLR2-Cell-Lysate-Western-Blot-NBP2-30165-img0001.jpg "Western Blot: TLR2 Cell Lysate [NBP2-30165] - 15 ul of mouse TLR2 transfected cell lysate tested with IMG-526 (A) and IMG-662 (B).")
![Western Blot: TLR2 Cell Lysate [NBP2-25060] - 20 ul of human TLR2 transfected cell lysate tested with 2 ug/ml TLR2 Antibody](http://images.novusbio.com/fullsize/TLR2-Cell-Lysate-Western-Blot-NBP2-25060-img0001.jpg "Western Blot: TLR2 Cell Lysate [NBP2-25060] - 20 ul of human TLR2 transfected cell lysate tested with 2 ug/ml TLR2 Antibody")