ZEB1 Antibody


Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP1-88845] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human smooth muscle shows strong nuclear positivity in myocytes.
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Immunohistochemical staining of human endometrium shows strong nuclear positivity in stromal cells.
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Analysis in human cerebral cortex and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human cerebral cortex shows strong nuclear positivity in glial cells.
Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human glioma shows strong nuclear positivity in tumor cells.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
0.2 mg/ml
Validated by:

Orthogonal Strategies


Order Details

ZEB1 Antibody Summary

Mesenchymal Cells Marker
Specificity of human ZEB1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. Western Blot reactivity reported in scientific literature (PMID: 26455323)
Theoretical MW
124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ZEB1 Protein (NBP1-88845PEP)
Read Publications using
NBP1-88845 in the following applications:

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22482439).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2) and 40% Glycerol
0.02% Sodium Azide
0.2 mg/ml
Immunogen affinity purified

Alternate Names for ZEB1 Antibody

  • AREB6
  • AREB6MGC133261
  • BZP
  • delta-crystallin enhancer binding factor 1
  • FECD6
  • Negative regulator of IL2
  • NIL-2-A zinc finger protein
  • NIL2A
  • NIL-2-A
  • posterior polymorphous corneal dystrophy 3
  • PPCD3
  • TCF8
  • TCF-8
  • transcription factor 8 (represses interleukin 2 expression)
  • Transcription factor 8
  • ZEB
  • ZEB1
  • ZFHX1A
  • zinc finger E-box binding homeobox 1
  • zinc finger E-box-binding homeobox 1
  • zinc finger homeodomain enhancer-binding protein


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ZEB1 Antibody (NBP1-88845)(19)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ChIP, ICC/IF, IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NBP1-88845 Applications Species
Yao X, Sun S, Zhou X et al. Clinicopathological significance of ZEB-1 and E-cadherin proteins in patients with oral cavity squamous cell carcinoma Onco Targets Ther 2017 Feb 28 [PMID: 28243114] (IHC-P, Human) IHC-P Human
A Barbachano, A Fernandez-Barral, F Pereira et al. SPROUTY-2 represses the epithelial phenotype of colon carcinoma cells via upregulation of ZEB1 mediated by ETS1 and miR-200/miR-150. Oncogene 2015 Oct 12 [PMID: 26455323] (WB, Human) WB Human
Asnaghi L, Gezgin G, Tripathy A et al. EMT-associated factors promote invasive properties of uveal melanoma cells. Mol Vis 2015 [PMID: 26321866] (WB, Human) WB Human
Mock K, Preca BT, Brummer T et al. The EMT-activator ZEB1 induces bone metastasis associated genes including BMP-inhibitors. Oncotarget 2015 Jun 10 [PMID: 25973542] (WB, Human) WB Human
Meidhof S, Brabletz S, Lehmann W et al. ZEB1-associated drug resistance in cancer cells is reversed by the class I HDAC inhibitor mocetinostat. EMBO Mol Med 2015 Apr 14 [PMID: 25872941] (IHC, WB, ICC/IF, Human) IHC, WB, ICC/IF Human
Claudio Isella, Andrea Terrasi, Sara Erika Bellomo et al. Stromal contribution to the colorectal cancer transcriptome. Nature Genetics 2015 Feb 23 [PMID: 25706627] (IHC, Human) IHC Human
Davidson B, Holth A, Hellesylt E et al. The clinical role of epithelial-mesenchymal transition and stem cell markers in advanced-stage ovarian serous carcinoma effusions. Hum Pathol 2015 Jan [PMID: 25455994] (IHC, Human) IHC Human
Ceppi P, Hadji A, Kohlhapp FJ et al. CD95 and CD95L promote and protect cancer stem cells. Nat Commun 2014 Nov 4 [PMID: 25366259] (WB, ICC/IF, Human) WB, ICC/IF Human
Zeindl-Eberhart E, Brandl L, Liebmann S et al. Epithelial-Mesenchymal Transition Induces Endoplasmic-Reticulum-Stress Response in Human Colorectal Tumor Cells. PLoS One 2014 [PMID: 24498091]
Roca H, Hernandez J, Weidner S et al. Transcription Factors OVOL1 and OVOL2 Induce the Mesenchymal to Epithelial Transition in Human Cancer. PLoS One 2013 [PMID: 24124593]
Show All 19 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZEB1 Antibody (NBP1-88845). (Showing 1 - 1 of 1 FAQs).

  1. In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
    • ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.

Secondary Antibodies


Isotype Controls

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Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Beta Catenin in Cell Adhesion and T-cell Signaling
Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly associates with cell surface cadherin glycoproteins. It is one member of the catenin family that includes alpha Catenin, beta Catenin, and gamma Catenin. Colocalization studies usi...  Read full blog post.

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Gene Symbol ZEB1