GLI-1 Antibody [Alexa Fluor® 405]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 405

GLI-1 Antibody [Alexa Fluor® 405] Summary

Immunogen
A synthetic peptide made to an internal portion of the human Gli1 protein (between residues 150-200) [UniProt P08151]
Localization
Cytoplasm. Nucleus. Note: Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This Gli1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, and Western Blot. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for GLI-1 Antibody [Alexa Fluor® 405]

  • GLI family zinc finger 1
  • GLI1
  • GLI-1
  • glioma-associated oncogene family zinc finger 1
  • glioma-associated oncogene homolog 1 (zinc finger protein)
  • Glioma-associated oncogene
  • GLIzinc finger protein GLI1
  • Oncogene GLI

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GLI-1 Antibody (NBP1-78259AF405) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-78259AF350
Alexa Fluor 405 NBP1-78259AF405
Alexa Fluor 488 NBP1-78259AF488
Alexa Fluor 532 NBP1-78259AF532
Alexa Fluor 594 NBP1-78259AF594
Alexa Fluor 647 NBP1-78259AF647
Alexa Fluor 700 NBP1-78259AF700
Alexa Fluor 750 NBP1-78259AF750
Biotin NBP1-78259B
DyLight 350 NBP1-78259UV
DyLight 405 NBP1-78259V
DyLight 488 NBP1-78259G
DyLight 550 NBP1-78259R
DyLight 650 NBP1-78259C
DyLight 680 NBP1-78259FR
DyLight 755 NBP1-78259IR
FITC NBP1-78259F
HRP NBP1-78259H
Janelia Fluor 549 NBP1-78259JF549
Janelia Fluor 646 NBP1-78259JF646

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Bioinformatics Tool for GLI-1 Antibody (NBP1-78259AF405)

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Blogs on GLI-1

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol GLI1