Lgr5/GPR49 Antibody [DyLight 488]

• Reactivity: Hu, Mu, Po
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 488

Lgr5/GPR49 Antibody [DyLight 488] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Synthetic peptide made to an internal portion of the human GPR49/LGR5 protein (within residues 650-700). [Swiss-Prot: O75473]
• Localization: Cell-membrane; Multi-pass membrane protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LGR5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP1-28904. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32016468).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Lgr5/GPR49 Antibody [DyLight 488]

• FEX
• G protein-coupled receptor 49
• GPR49
• GPR49G-protein coupled receptor HG38
• GPR67
• G-protein coupled receptor 49
• G-protein coupled receptor 67
• GRP49
• HG38
• leucine-rich repeat containing G protein-coupled receptor 5
• leucine-rich repeat-containing G protein-coupled receptor 5
• leucine-rich repeat-containing G-protein coupled receptor 5
• Lgr5
• MGC117008
• orphan G protein-coupled receptor HG38

Background

GPR49 is an orphaned member of the glycoprotein hormone receptor subfamily. It is thought to play an important role in growth and differentiation of embyronic tissue. It is highly expressed in the brain, spinal cord, placenta, and skeletal muscle. GPR49 is up-regulated in several cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lgr5/GPR49 Antibody (NBP1-28904G)(1)

Publication using NBP1-28904G

• Johnson Chacko L, Sergi C, Eberharter T et al. Early appearance of key transcription factors influence the spatiotemporal development of the human inner ear Cell Tissue Res. 2019-12-02 [PMID: 31788757]

Bioinformatics

• Gene Symbol: LGR5