NHE3/SLC9A3 [p Ser552] Antibody (14D5) [DyLight 550]

• Reactivity: Hu, Mu, Rt, Po, Ma-Op, Pm, Rb
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, KO, Mycoplasma
• Clone: 14D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 550

NHE3/SLC9A3 [p Ser552] Antibody (14D5) [DyLight 550] Summary

• Immunogen: Synthetic peptide made to a region surrounding the Serine 552 portion of rat NHE3. [UniProt# P26433]
• Modification: p Ser552
• Specificity: This antibody detects NHE3 posphorylated at Serine 552, and does not cross-react with the non-phosphopeptide.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC9A3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Knockdown Validated
  • Knockout Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 29510506).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for NHE3/SLC9A3 [p Ser552] Antibody (14D5) [DyLight 550]

• isoform 3
• MGC126718
• NHE3
• NHE3MGC126720
• SLC9A3
• solute carrier family 9 (sodium/hydrogen exchanger), member 3
• Solute carrier family 9 member 3

Background

NHE3 is a member of the Sodium/Hydrogen exchanger family that plays a crucial role in acid-base and volume homeostasis by mediating the majority of sodium and bicarbonate reabsorption in the proximal tubule of the kidney. Two PKA consensus sites have been discovered in rat NHE3, serine 552 and serine 605. Research suggests that dopamine-induced phosphorylation of these consensus sites inhibit NHE3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NHE3/SLC9A3 Antibody (NB110-81529R)(1)

Publication using NB110-81529R

• Gaibee Z, Warner N, Gwilt K et al. The Genetic Architecture of Congenital Diarrhea and Enteropathy The New England journal of medicine 2025-04-03 [PMID: 40174224]

Bioinformatics

• Gene Symbol: SLC9A3