Notch-1 Antibody (mN1A)

Images

 
Genetic Strategies: Knockout Validated: Notch-1 Antibody (mN1A) [NB100-78486] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. ...read more
Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Cell extracts from Jurkat (Lane 1) or mouse thymocytes (Lane 2) were Western Blot analyzed with monoclonal anti-NOTCH1 antibody. The mN1A antibody recognizes both ...read more
Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (mN1A) [NB100-78486] - The Notch1 Antibody was tested in HEK293 cells at a 1:50 dilution against Dylight 488 (Green). Actin was counterstained against ...read more
Immunohistochemistry-Paraffin: Notch-1 Antibody (mN1A) [NB100-78486] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human pancreatic cancer using 1:10 dilution of Notch-1 antibody on a Bond Rx autostainer ...read more
Flow Cytometry: Notch-1 Antibody (mN1A) [NB100-78486] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using Notch1 antibody (dark blue). Isotype control shown in orange. An antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, KO
Clone
mN1A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Notch-1 Antibody (mN1A) Summary

Immunogen
Mouse Notch1 protein [UniProt Q01705]
Specificity
Does not cross-react with Notch 2, 3, or 4.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NOTCH1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:100
  • CyTOF-ready
  • Knockout Validated
Application Notes
The mN1A monoclonal antibody reacts with the intracellular domain of mouse and human Notch1 and has been reported to have highest affinity for activated intracellular Notch1 and lower affinity for full-length unprocessed/heterodimeric Notch1 forms. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
272.505 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Notch-1 Lysate (NBL1-13725)
Notch-1 Lysate (NBP2-64906)
Reviewed Applications
Read 1 Review rated 5
using
NB100-78486 in the following applications:

Publications
Read Publications using
NB100-78486 in the following applications:

  • IHC
    2 publications
  • IP
    1 publication
  • WB
    3 publications

Reactivity Notes

Mouse and human. Does not recognize rat Notch1.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Notch-1 Antibody (mN1A)

  • EC 2.1.2.11
  • EC 3.4.21.68
  • hN1
  • Notch (Drosophila) homolog 1 (translocation-associated)
  • notch 1Notch homolog 1, translocation-associated (Drosophila)
  • Notch homolog 1, translocation-associated
  • Notch1
  • Notch-1
  • TAN1
  • TAN1neurogenic locus notch homolog protein 1
  • Translocation-associated notch protein TAN-1

Background

Notch1, also known as neurogenic locus notch homolog protein 1, is a single-pass transmembrane protein that serves as the receptor for the Delta1, Jagged1 and Jagged2 ligands. Activation of Notch1 causes the cytoplasmic domain to be cleaved, releasing the notch intracellular domain (NICD) which forms a complex with RBPJ/RBPSUH and subsequently influences transcription of its target genes. Because the majority of Notch1 ligands are transmembrane proteins, Notch1 is primarily triggered by cell-to-cell contact and employs lateral inhibition mechanisms to influence local populations of cells. The Notch1 pathway plays a pivotal role in development, including cell-fate determination, proliferation, and cell contact-dependent signaling. Aberrant activation of the Notch1 pathway has been associated with numerous diseases such as bicuspid aortic valve and cancer - in particular certain leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Notch-1 Antibody (NB100-78486)(6)

Review for Notch-1 Antibody (NB100-78486) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB100-78486:
Filter by Applications
WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Shyam Gudey
WB Other 01/10/2014
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Summary

ApplicationWestern Blot
SpeciesOther
LotLot B 134275
Special ApplicationsImmunoprecipitation, Immunofluroscence

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Notch-1 Antibody (NB100-78486) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Notch-1 Antibody (NB100-78486)

Discover related pathways, diseases and genes to Notch-1 Antibody (NB100-78486). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Notch-1 Antibody (NB100-78486)

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Pathways for Notch-1 Antibody (NB100-78486)

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PTMs for Notch-1 Antibody (NB100-78486)

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Research Areas for Notch-1 Antibody (NB100-78486)

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Blogs on Notch-1.

Notch1 - A multifunctional transmembrane receptor
Notch1 is a member of the Notch family of Type 1 single-pass transmembrane proteins that share an extracellular domain of multiple epidermal growth factor-like (EGF) repeats. Notch family members play key roles in a variety of developmental process...  Read full blog post.

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Recent Reviews

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1
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1
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Shyam Gudey
01/10/2014
Application: WB
Species: Other

Bioinformatics

Gene Symbol NOTCH1
Entrez
Uniprot