Notch-1 Antibody (mN1A) - BSA Free

Images

 
Genetic Strategies: Knockout Validated: Notch-1 Antibody (mN1A) [NB100-78486] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. PVDF membrane was ...read more
Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Cell extracts from Jurkat (Lane 1) or mouse thymocytes (Lane 2) were analyzed with monoclonal anti-NOTCH1 antibody. The mN1A antibody recognizes both mouse and human ...read more
Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (mN1A) [NB100-78486] - The Notch1 Antibody was tested in HEK293 cells at a 1:50 dilution with DyLight 488 (Green). Actin was counterstained with Phalloidin 568 ...read more
Immunohistochemistry-Paraffin: Notch-1 Antibody (mN1A) [NB100-78486] - Analysis of FFPE human pancreatic cancer using 1:10 dilution of Notch-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 ...read more
Flow Cytometry: Notch-1 Antibody (mN1A) [NB100-78486] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using Notch1 antibody (dark blue). Isotype control shown in orange. An antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, KO
Clone
mN1A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

Notch-1 Antibody (mN1A) - BSA Free Summary

Immunogen
Mouse Notch1 protein [Uniprot: Q01705]
Specificity
Does not cross-react with Notch 2, 3, or 4.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NOTCH1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:100
  • Knockout Validated
  • Western Blot 1 - 2 ug/mL
Application Notes
The mN1A monoclonal antibody reacts with the intracellular domain of mouse and human Notch1 and has been reported to have highest affinity for activated intracellular Notch1 and lower affinity for full-length unprocessed/heterodimeric Notch1 forms.

This antibody is CyTOF ready.
Theoretical MW
272.505 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Notch-1 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-78486 in the following applications:

Publications
Read Publications using
NB100-78486 in the following applications:

  • 1 publication
  • IHC
    3 publications
  • 1 publication
  • IP
    1 publication
  • WB
    5 publications

Reactivity Notes

Does not recognize rat Notch1.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Notch-1 Antibody (mN1A) - BSA Free

  • EC 2.1.2.11
  • EC 3.4.21.68
  • hN1
  • Notch (Drosophila) homolog 1 (translocation-associated)
  • notch 1Notch homolog 1, translocation-associated (Drosophila)
  • Notch homolog 1, translocation-associated
  • Notch1
  • Notch-1
  • TAN1
  • TAN1neurogenic locus notch homolog protein 1
  • Translocation-associated notch protein TAN-1

Background

Notch1, also known as neurogenic locus notch homolog protein 1, is a single-pass transmembrane protein that serves as the receptor for the Delta1, Jagged1 and Jagged2 ligands. Activation of Notch1 causes the cytoplasmic domain to be cleaved, releasing the notch intracellular domain (NICD) which forms a complex with RBPJ/RBPSUH and subsequently influences transcription of its target genes. Because the majority of Notch1 ligands are transmembrane proteins, Notch1 is primarily triggered by cell-to-cell contact and employs lateral inhibition mechanisms to influence local populations of cells. The Notch1 pathway plays a pivotal role in development, including cell-fate determination, proliferation, and cell contact-dependent signaling. Aberrant activation of the Notch1 pathway has been associated with numerous diseases such as bicuspid aortic valve and cancer - in particular certain leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-78486
Species: Hu, Mu, Rt(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, KO

Publications for Notch-1 Antibody (NB100-78486)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IP, WB.


Filter By Application
ICC/IF
(1)
IHC
(3)
IHC-Fr
(1)
IP
(1)
WB
(5)
All Applications
Filter By Species
Human
(1)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB100-78486 Applications Species
Alghamdi TA, Krentz NAJ, Smith N et al. Zmiz1 is required for mature B-cell function and mass expansion upon high fat feeding Molecular metabolism Oct 25 2022 [PMID: 36307047] (WB, Mouse)

Details:
Dilution used in WB 1:200
WB Mouse
Gong M, Yan F, Yu L, Li F A dopamine-methacrylated hyaluronic acid hydrogel as an effective carrier for stem cells in skin regeneration therapy Cell death & disease Aug 27 2022 [PMID: 36030275] (WB, IHC-Fr, Mouse)

Details:
WB Dilutions: 1:1000; ICC/IF Dilutions: 1:100
WB, IHC-Fr Mouse
Bai J, Khajavi M, Sui L et al. Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes Angiogenesis Sep 21 2020 [PMID: 32955682] (ICC/IF) ICC/IF
Kang H, Yang K, Xiao L et al. Osteoblast Hypoxia-Inducible Factor-1a Pathway Activation Restrains Osteoclastogenesis via the Interleukin-33-MicroRNA-34a-Notch1 Pathway. Front Immunol. 2017 Oct 31 [PMID: 29085370] (WB, Mouse) WB Mouse
Singh NK, Kotla S, Kumar R, Rao GN Cyclic AMP Response Element Binding Protein Mediates Pathological Retinal Neovascularization via Modulating DLL4-NOTCH1 Signaling. EBioMedicine 2015 Nov [PMID: 26870802]
Qiu, H, Tang, X Et al. Notch1 Autoactivation via Transcriptional Regulation of Furin, Which Sustains Notch1 Signaling by Processing Notch1-Activating Proteases ADAM10 and Membrane Type 1 Matrix Metalloproteinase. Mol Cell Biol Nov 1 2015 [PMID: 26283728] (IHC, Mouse) IHC Mouse
Jundt F et al. Leukemia. 22:1587. 2008 [PMID: 18449208] (IHC) IHC
Sun H et al. J. Cell Biol. 177:647. 2007 [PMID: 17502421] (IHC) IHC
De Strooper B, Annaert W, Cupers P et al. A presenilin-1-dependent gamma-secretase-like protease mediates release of Notch intracellular domain. Nature. 1999 Apr 8 [PMID: 10206645] (WB, Mouse) WB Mouse
Ray WJ, Yao M, Nowotny P et al. Evidence for a physical interaction between presenilin and Notch. Proc Natl Acad Sci U S A. 1999 Mar 16 [PMID: 10077672] (WB, IP, Human) WB, IP Human

Reviews for Notch-1 Antibody (NB100-78486) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.

Reviews using NB100-78486:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Notch-1 NB100-78486
Enlarge
5
reviewed by:
Raj Kumar
WB Mouse 12/07/2021
View

Summary

ApplicationWestern Blot
Sample TestedVascular Smooth Muscle Cells
SpeciesMouse
LotNR1C2
  5
reviewed by:
Verified Customer
WB Other 01/10/2014
View

Summary

ApplicationWestern Blot
SpeciesOther
LotLot B 134275

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Notch-1 Antibody (NB100-78486). (Showing 1 - of FAQs).

    Control Lysate(s)

    Secondary Antibodies

     

    Isotype Controls

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    Recent Reviews

    5
    5
    2
    4
    0
    3
    0
    2
    0
    1
    0

    Raj Kumar
    12/07/2021
    Application: WB
    Species: Mouse

     
    Verified Customer
    01/10/2014
    Application: WB
    Species: Other

    Bioinformatics

    Gene Symbol NOTCH1
    Entrez
    Uniprot