Notch-1 Antibody (mN1A)

Images

 
Genetic Strategies: Knockout Validated: Notch-1 Antibody (mN1A) [NB100-78486] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Notch-1 knockout (KO) HeLa cell line. PVDF membrane was ...read more
Western Blot: Notch-1 Antibody (mN1A) [NB100-78486] - Cell extracts from Jurkat (Lane 1) or mouse thymocytes (Lane 2) were analyzed with monoclonal anti-NOTCH1 antibody. The mN1A antibody recognizes both mouse and human ...read more
Immunocytochemistry/ Immunofluorescence: Notch-1 Antibody (mN1A) [NB100-78486] - The Notch1 Antibody was tested in HEK293 cells at a 1:50 dilution with DyLight 488 (Green). Actin was counterstained with Phalloidin 568 ...read more
Immunohistochemistry-Paraffin: Notch-1 Antibody (mN1A) [NB100-78486] - Analysis of FFPE human pancreatic cancer using 1:10 dilution of Notch-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 ...read more
Flow Cytometry: Notch-1 Antibody (mN1A) [NB100-78486] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and MCF-7 (B) cells using Notch1 antibody (dark blue). Isotype control shown in orange. An antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, KO
Clone
mN1A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

Notch-1 Antibody (mN1A) Summary

Immunogen
Mouse Notch1 protein [Uniprot: Q01705]
Specificity
Does not cross-react with Notch 2, 3, or 4.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NOTCH1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/mL
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:100
  • CyTOF-ready
  • Knockout Validated
Application Notes
The mN1A monoclonal antibody reacts with the intracellular domain of mouse and human Notch1 and has been reported to have highest affinity for activated intracellular Notch1 and lower affinity for full-length unprocessed/heterodimeric Notch1 forms.

This antibody is CyTOF ready.
Theoretical MW
272.505 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Notch-1 Overexpression Lysate
Notch-1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-78486 in the following applications:

Publications
Read Publications using
NB100-78486 in the following applications:

  • 1 publication
  • IHC
    2 publications
  • IP
    1 publication
  • WB
    3 publications

Reactivity Notes

Does not recognize rat Notch1.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Notch-1 Antibody (mN1A)

  • EC 2.1.2.11
  • EC 3.4.21.68
  • hN1
  • Notch (Drosophila) homolog 1 (translocation-associated)
  • notch 1Notch homolog 1, translocation-associated (Drosophila)
  • Notch homolog 1, translocation-associated
  • Notch1
  • Notch-1
  • TAN1
  • TAN1neurogenic locus notch homolog protein 1
  • Translocation-associated notch protein TAN-1

Background

Notch1, also known as neurogenic locus notch homolog protein 1, is a single-pass transmembrane protein that serves as the receptor for the Delta1, Jagged1 and Jagged2 ligands. Activation of Notch1 causes the cytoplasmic domain to be cleaved, releasing the notch intracellular domain (NICD) which forms a complex with RBPJ/RBPSUH and subsequently influences transcription of its target genes. Because the majority of Notch1 ligands are transmembrane proteins, Notch1 is primarily triggered by cell-to-cell contact and employs lateral inhibition mechanisms to influence local populations of cells. The Notch1 pathway plays a pivotal role in development, including cell-fate determination, proliferation, and cell contact-dependent signaling. Aberrant activation of the Notch1 pathway has been associated with numerous diseases such as bicuspid aortic valve and cancer - in particular certain leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, KO

Publications for Notch-1 Antibody (NB100-78486)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IP, WB.


Filter By Application
ICC/IF
(1)
IHC
(2)
IP
(1)
WB
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
All Species

Review for Notch-1 Antibody (NB100-78486) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB100-78486:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Shyam Gudey
WB Other 01/10/2014
View

Summary

ApplicationWestern Blot
SpeciesOther
LotLot B 134275
Special ApplicationsImmunoprecipitation, Immunofluroscence

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Notch-1 Antibody (NB100-78486). (Showing 1 - of FAQs).

    Control Lysate(s)

    Secondary Antibodies

     

    Isotype Controls

    Other Available Formats

    Alexa Fluor 350 NB100-78486AF350
    Alexa Fluor 405 NB100-78486AF405
    Alexa Fluor 488 NB100-78486AF488
    Alexa Fluor 532 NB100-78486AF532
    Alexa Fluor 594 NB100-78486AF594
    Alexa Fluor 647 NB100-78486AF647
    Alexa Fluor 700 NB100-78486AF700
    Alexa Fluor 750 NB100-78486AF750
    Allophycocyanin NB100-78486APC
    DyLight 350 NB100-78486UV
    DyLight 405 NB100-78486V
    DyLight 488 NB100-78486G
    DyLight 550 NB100-78486R
    DyLight 594 NB100-78486DL594
    DyLight 650 NB100-78486C
    DyLight 680 NB100-78486FR
    DyLight 755 NB100-78486IR
    FITC NB100-78486F
    HRP NB100-78486H
    Janelia Fluor 549 NB100-78486JF549
    Janelia Fluor 646 NB100-78486JF646
    PerCP NB100-78486PCP

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    Blogs on Notch-1.

    Notch1 - A multifunctional transmembrane receptor
    Notch1 is a member of the Notch family of Type 1 single-pass transmembrane proteins that share an extracellular domain of multiple epidermal growth factor-like (EGF) repeats. Notch family members play key roles in a variety of developmental process...  Read full blog post.

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    Recent Reviews

    5
    5
    1
    4
    0
    3
    0
    2
    0
    1
    0

     
    Shyam Gudey
    01/10/2014
    Application: WB
    Species: Other

    Bioinformatics

    Gene Symbol NOTCH1
    Entrez
    Uniprot