Lgr5/GPR49 Antibody


Immunocytochemistry/ Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP1-28904] - Wnt-related genes selectively expressed in human adrenal ZG. IHC localization of Lgr5/GPR49 & Wnt signaling proteins in ZG: canonical beta-catenin ...read more
Flow (Intracellular): Lgr5/GPR49 Antibody [NBP1-28904] - An intracellular stain was performed on HepG2 cells with Lgr5/GPR49 Antibody NBP1-28904AF647 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Analysis of human small intestine.
Immunohistochemistry-Frozen: Lgr5/GPR49 Antibody [NBP1-28904] - Staining on pig small intestine. Image from verified customer review.

Product Details

Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
1.0 mg/ml

Lgr5/GPR49 Antibody Summary

Synthetic peptide made to an internal portion of the human GPR49/LGR5 protein (within residues 650-700). [Swiss-Prot: O75473]
Cell-membrane; Multi-pass membrane protein.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1.0-2.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 2-5 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Flow (Intracellular)
Lgr5/GPR49 Knockout HeLa Cell Lysate
Control Peptide
Lgr5/GPR49 Peptide (NBP1-28904PEP)
Reviewed Applications
Read 1 Review rated 4
NBP1-28904 in the following applications:

Read Publications using
NBP1-28904 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32016468).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for Lgr5/GPR49 Antibody

  • FEX
  • G protein-coupled receptor 49
  • GPR49
  • GPR49G-protein coupled receptor HG38
  • GPR67
  • G-protein coupled receptor 49
  • G-protein coupled receptor 67
  • GRP49
  • HG38
  • leucine-rich repeat containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G-protein coupled receptor 5
  • Lgr5
  • MGC117008
  • orphan G protein-coupled receptor HG38


GPR49/LGR5 (G protein-coupled receptor 49 / leucine-rich repeat-containing G protein-coupled receptor 5), also called FEX, GPR67, GRP49, HG38 or MGC117008), is structurally related to members of glycoprotein hormone receptor family including FSH-R, TSH-R and LH-R. LGRs (leucine-rich repeat-containing G-protein coupled receptor) are divided into three major subgroups: glycoprotein hormone receptors; the subgroup of GPR48, GPR49, and LGR6; and receptors of relaxin family ligands (LGR7 and LGR8), of which GPR49 is emerging as a significant player in stem cells biology as it is highly expressed on stem cells in small intestine, colon and hair follicle. Deficiency of GPR49 in mice has been shown to cause neonatal lethality characterized by ankyloglossia, gastrointestinal distension and induction of premature differentiation of Paneth cells. LGR5 is a downstream target gene of Wnt signaling pathway (critical for morphogenesis and tumorigenesis) and it has also been reported as a receptor for R-spondins which are potent mediators of Wnt/beta-catenin as well as Wnt/PCP signaling. Accordingly, when compared to normal tissues, LGR5 is found upregulated in certain cancers e.g liver cancer with beta-catenin mutations, basal cell carcinoma, colon and ovarian carcinomas.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Po
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, Flow-IC
Species: Hu, Mu
Applications: WB, ChIP, Flow, ICC/IF, IP, Flow-IC
Species: Hu, Mu
Applications: IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC, ICC
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Po, Bv, Ca, Eq, Pm, Pm, Rb
Applications: IHC, IHC-P
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Flow, ICC/IF, IHC
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
Species: Hu, Mu, Rt, Ca
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-Fr
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Po
Applications: WB, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Lgr5/GPR49 Antibody (NBP1-28904)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-28904 Applications Species
von Erlach T, Saxton S, Shi Y et al. Robotically handled whole-tissue culture system for the screening of oral drug formulations Nat Biomed Eng Apr 27 2020 [PMID: 32341538]
Xie Y, Ding F, Di W et al. Impact of a high fat diet on intestinal stem cells and epithelial barrier function in middle aged female mice Mol Med Report Jan 13 2020 [PMID: 32016468] (ICC/IF, Mouse) ICC/IF Mouse
Kim MS, Cho HI, Yoon HJ et al. JIB-04, A Small Molecule Histone Demethylase Inhibitor, Selectively Targets Colorectal Cancer Stem Cells by Inhibiting the Wnt/B-Catenin Signaling Pathway Sci Rep Apr 26 2018 [PMID: 29700375] (WB, Human) WB Human
Cervello I, Gil-Sanchis C, Santamaria X et al. Leucine-rich repeat-containing G-protein-coupled receptor 5-positive cells in the endometrial stem cell niche. Fertil. Steril. Nov 22 2016 [PMID: 27887719]
Shaikh LH, Zhou J, Teo AE et al. LGR5 activates non-canonical Wnt-signaling and inhibits aldosterone production in the human adrenal J. Clin. Endocrinol. Metab. 2015 Apr 27 [PMID: 25915569] (IHC-P, Human)

Lgr5/GPR49 antibody used for Immunohistochemistry on formalin-fixed, paraffin-embedded human adrenal sections.
IHC-P Human
Jung K, Saif LJ. Porcine epidemic diarrhea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis. Vet J. 2015 Feb 26 [PMID: 25841898] (IHC-Fr, Porcine)

GPR49/LGR5 antibody was used in IHC-Fr application for studying the immunolocalization of LGR5+ crypt stem cells in the small intestine of gnotobiotic pigs inoculated with US porcine epidemic diarrhea virus/PEDV strain PC21A. Fig 4 depicts the LGR5+ crypt stem cells and LGR5+ positive crypt base columnar cells in the Jejunum section of a PEDV-inoculated pig at post-inoculation hour 30, and this data confirms the presence of stem cells which are highly important as far as the epithelial cell renewal during acute-stage of PEDV infection is concerned.
IHC-Fr Porcine
Di W, Lv Y, Xia F et al. Improvement of intestinal stem cells and barrier function via calorie restriction in middle-aged C57BL/6 mice Nutrition Research Jun 1 2020 (IHC, Mouse) IHC Mouse

Review for Lgr5/GPR49 Antibody (NBP1-28904) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-28904:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Lgr5/GPR49 NBP1-28904
reviewed by:
Kwonil Jung
IHC-Fr Other 01/14/2014


Sample TestedPig small intestine


Blocking Details1x Powerblocking solution (Casein) in distilled water (Biogenex) for 30 mins at room temp

Primary Anitbody

Dilution Ratio1:30 in PBS including 0.1% tween 20 overnight

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, Alexa Fluor 594
Secondary Manufacturer Cat#A-11012 Invitrogen
Secondary Concentration1:200 in PBS


Detection NotesFluorescence microscpy examination
Fixation DetailsFrozen tissues are fixed in paraforaldehyde buffer for 20 min, without antigen retrieval step.
Wash DescriptionVigorous wash with PBS

Product General Protocols

View specific protocols for Lgr5/GPR49 Antibody (NBP1-28904): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lgr5/GPR49 Antibody (NBP1-28904). (Showing 1 - 3 of 3 FAQs).

  1. This morning I have a customer who has purchased NBP1-28904 and would like to test it in flow cytometry. She is new to flow and is wondering if you might have a recommended protocol. Also, I see that the immunogen is within aa 650-700 which appears to span both helical and cytoplasmic domains. Do you know if the exact immunogen is fully within one domain or the other? Is permeabilization required? Any information you have is much appreciated.
    • Unfortunately I cannot disclose anything more about the immunogen sequence; however, our lab does recommend permeabilization for using this antibody. This is our general flow cytometry protocol. Note that if she tests this antibody in flow, she would be eligible for our Innovators Reward Program.
  2. Is it possible to confirm that the actual immunogen is taken from within the actual cytoplasmic domain (aa 660-682)?
    • Yes, I can confirm that the immunogen falls within the cytoplasmic domain of this protein.
  3. Please inform us this item NBP1-28904 is stain cytoplasmic or stain Nuclus?
    • NBP1-28904 detects Lgr5/GPR49 protein which localize to Cell membrane and the Golgi apparatus. Therefore, this antibody should generate a staining pattern with more signal in membranes and in cytoplasm as patches around the nucleus of the cells. More information on this protein may be found at Uniprot site

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-28904AF350
Alexa Fluor 405 NBP1-28904AF405
Alexa Fluor 488 NBP1-28904AF488
Alexa Fluor 532 NBP1-28904AF532
Alexa Fluor 594 NBP1-28904AF594
Alexa Fluor 647 NBP1-28904AF647
Alexa Fluor 700 NBP1-28904AF700
Alexa Fluor 750 NBP1-28904AF750
Biotin NBP1-28904B
DyLight 350 NBP1-28904UV
DyLight 405 NBP1-28904V
DyLight 488 NBP1-28904G
DyLight 550 NBP1-28904R
DyLight 594 NBP1-28904DL594
DyLight 650 NBP1-28904C
DyLight 680 NBP1-28904FR
DyLight 755 NBP1-28904IR
FITC NBP1-28904F
HRP NBP1-28904H
Janelia Fluor 549 NBP1-28904JF549
Janelia Fluor 646 NBP1-28904JF646

Additional Lgr5/GPR49 Products

Bioinformatics Tool for Lgr5/GPR49 Antibody (NBP1-28904)

Discover related pathways, diseases and genes to Lgr5/GPR49 Antibody (NBP1-28904). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Lgr5/GPR49 Antibody (NBP1-28904)

Discover more about diseases related to Lgr5/GPR49 Antibody (NBP1-28904).

Pathways for Lgr5/GPR49 Antibody (NBP1-28904)

View related products by pathway.

PTMs for Lgr5/GPR49 Antibody (NBP1-28904)

Learn more about PTMs related to Lgr5/GPR49 Antibody (NBP1-28904).

Research Areas for Lgr5/GPR49 Antibody (NBP1-28904)

Find related products by research area.

Blogs on Lgr5/GPR49.

Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Recent Reviews


Kwonil Jung
Application: IHC-Fr
Species: Other


Gene Symbol LGR5