Lgr5/GPR49 Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: Lgr5/GPR49 Antibody [NBP1-28904] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Wnt-related genes show selective expression in the ZG of human adrenal. IHC localization of LGR5 in the ZG. IHC was performed on formalin-fixed, paraffin-embedded ...read more
Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Analysis of human small intestine.
Wnt-related genes show selective expression in the ZG of human adrenal. B) IHC localization of LGR5 and downstream Wnt signaling proteins in the ZG: from the canonical beta-catenin pathway, beta-catenin and LEF1, and ...read more

Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Lgr5/GPR49 Antibody - BSA Free Summary

Immunogen
Synthetic peptide made to an internal portion of the human GPR49/LGR5 protein (within residues 650-700). [Swiss-Prot: O75473]
Localization
Cell-membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LGR5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-5 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1.0-2.0 ug/ml
Control Peptide
Lgr5/GPR49 Peptide (NBP1-28904PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-28904 in the following applications:

Publications
Read Publications using
NBP1-28904 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32016468).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lgr5/GPR49 Antibody - BSA Free

  • FEX
  • G protein-coupled receptor 49
  • GPR49
  • GPR49G-protein coupled receptor HG38
  • GPR67
  • G-protein coupled receptor 49
  • G-protein coupled receptor 67
  • GRP49
  • HG38
  • leucine-rich repeat containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G-protein coupled receptor 5
  • Lgr5
  • MGC117008
  • orphan G protein-coupled receptor HG38

Background

GPR49/LGR5 (G protein-coupled receptor 49 / leucine-rich repeat-containing G protein-coupled receptor 5), also called FEX, GPR67, GRP49, HG38 or MGC117008), is structurally related to members of glycoprotein hormone receptor family including FSH-R, TSH-R and LH-R. LGRs (leucine-rich repeat-containing G-protein coupled receptor) are divided into three major subgroups: glycoprotein hormone receptors; the subgroup of GPR48, GPR49, and LGR6; and receptors of relaxin family ligands (LGR7 and LGR8), of which GPR49 is emerging as a significant player in stem cells biology as it is highly expressed on stem cells in small intestine, colon and hair follicle. Deficiency of GPR49 in mice has been shown to cause neonatal lethality characterized by ankyloglossia, gastrointestinal distension and induction of premature differentiation of Paneth cells. LGR5 is a downstream target gene of Wnt signaling pathway (critical for morphogenesis and tumorigenesis) and it has also been reported as a receptor for R-spondins which are potent mediators of Wnt/beta-catenin as well as Wnt/PCP signaling. Accordingly, when compared to normal tissues, LGR5 is found upregulated in certain cancers e.g liver cancer with beta-catenin mutations, basal cell carcinoma, colon and ovarian carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB120-16518
Species: Hu, Mu, Po, Rt
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-47386
Species: Hu, Mu, Rb(-)
Applications: ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP1-96140
Species: Hu, Mu
Applications: ChIP, Flow-IC, Flow, ICC/IF, IP, WB
MAB8458
Species: Hu
Applications: CyTOF-ready, Flow, ICC
AF2628
Species: Hu, Mu, Rt
Applications: ICC, IHC, WB
NBP1-77127
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
MAB7750
Species: Hu
Applications: Block, CyTOF-ready, Flow, ICC
AF1759
Species: Hu, Mu
Applications: ChIP, ICC, IP, Simple Western, WB
NBP1-54467
Species: Ch, Av-Du, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF1172
Species: Ca, Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
7268-CT
Species: Hu
Applications: BA
NB100-91662
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
DY413
Species: Mu
Applications: ELISA
NBP1-72042
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
7150-RS/CF
Species: Mu
Applications: BA
NB100-77903
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-Fr
NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-28904
Species: Hu, Mu, Po
Applications: WB, ICC/IF, IHC

Publications for Lgr5/GPR49 Antibody (NBP1-28904)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 3 applications: IHC-Fr, IHC-P, WB.


Filter By Application
IHC-Fr
(1)
IHC-P
(3)
WB
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-28904 Applications Species
Zhang R, Liu L, Wang F et al. AKAP8L enhances the stemness and chemoresistance of gastric cancer cells by stabilizing SCD1 mRNA Research Square 2022-08-25 [PMID: 36522343] (WB, Human) WB Human
Zhang R, Zhang X, Zhang W et al. Sohlh2 regulates the stemness and differentiation of colon cancer stem cells by downregulating LncRNA-H19 transcription Molecular cancer research : MCR 2022-10-26 [PMID: 36287177] (WB, Human) WB Human
Lee J, Kim JS, Cho HI et al. JIB-04, a Pan-Inhibitor of Histone Demethylases, Targets Histone-Lysine-Demethylase-Dependent AKT Pathway, Leading to Cell Cycle Arrest and Inhibition of Cancer Stem-Like Cell Properties in Hepatocellular Carcinoma Cells International journal of molecular sciences 2022-07-11 [PMID: 35887001] (WB, Human) WB Human
Akbari S, Kunter I, Azbazdar Y et al. LGR5/R-Spo1/Wnt3a axis promotes stemness and aggressive phenotype in hepatoblast-like hepatocellular carcinoma cell lines Cellular signalling 2021-03-06 [PMID: 33684507] (WB) WB
Di W, Lv Y, Xia F et al. Improvement of intestinal stem cells and barrier function via calorie restriction in middle-aged C57BL/6 mice Nutr Res 2020-09-03 [PMID: 32877836]
von Erlach T, Saxton S, Shi Y et al. Robotically handled whole-tissue culture system for the screening of oral drug formulations Nat Biomed Eng 2020-04-27 [PMID: 32341538]
Xie Y, Ding F, Di W et al. Impact of a high‑fat diet on intestinal stem cells and epithelial barrier function in middle‑aged female mice Mol Med Rep 2020-02-05 [PMID: 32016468] (IHC-P, IHC-P, Mouse) IHC-P, IHC-P Mouse
Kim MS, Cho HI, Yoon HJ et al. JIB-04, A Small Molecule Histone Demethylase Inhibitor, Selectively Targets Colorectal Cancer Stem Cells by Inhibiting the Wnt/B-Catenin Signaling Pathway Sci Rep 2018-04-26 [PMID: 29700375] (WB, Human) WB Human
Cervello I, Gil-Sanchis C, Santamaria X et al. Leucine-rich repeat-containing G-protein-coupled receptor 5-positive cells in the endometrial stem cell niche. Fertil. Steril. 2016-11-22 [PMID: 27887719]
Shaikh LH, Zhou J, Teo AE et al. LGR5 activates non-canonical Wnt-signaling and inhibits aldosterone production in the human adrenal J. Clin. Endocrinol. Metab. 2015-04-27 [PMID: 25915569] (IHC-P, Human)

Details:
Lgr5/GPR49 antibody used for Immunohistochemistry on formalin-fixed, paraffin-embedded human adrenal sections.
IHC-P Human
Show All 11 Publications.

Review for Lgr5/GPR49 Antibody (NBP1-28904) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-28904:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Lgr5/GPR49 NBP1-28904
Enlarge
4
reviewed by:
Kwonil Jung
IHC-Fr Other 01/14/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedPig small intestine
SpeciesOther

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lgr5/GPR49 Antibody (NBP1-28904). (Showing 1 - 3 of 3 FAQs).

  1. This morning I have a customer who has purchased NBP1-28904 and would like to test it in flow cytometry. She is new to flow and is wondering if you might have a recommended protocol. Also, I see that the immunogen is within aa 650-700 which appears to span both helical and cytoplasmic domains. Do you know if the exact immunogen is fully within one domain or the other? Is permeabilization required? Any information you have is much appreciated.
    • Unfortunately I cannot disclose anything more about the immunogen sequence; however, our lab does recommend permeabilization for using this antibody. This is our general flow cytometry protocol. Note that if she tests this antibody in flow, she would be eligible for our Innovators Reward Program.
  2. Is it possible to confirm that the actual immunogen is taken from within the actual cytoplasmic domain (aa 660-682)?
    • Yes, I can confirm that the immunogen falls within the cytoplasmic domain of this protein.
  3. Please inform us this item NBP1-28904 is stain cytoplasmic or stain Nuclus?
    • NBP1-28904 detects Lgr5/GPR49 protein which localize to Cell membrane and the Golgi apparatus . Therefore, this antibody should generate a staining pattern with more signal in membranes and in cytoplasm as patches around the nucleus of the cells. More information on this protein may be found at Uniprot site

Secondary Antibodies

 

Isotype Controls

Additional Lgr5/GPR49 Products

Diseases for Lgr5/GPR49 Antibody (NBP1-28904)

Discover more about diseases related to Lgr5/GPR49 Antibody (NBP1-28904).
 

Pathways for Lgr5/GPR49 Antibody (NBP1-28904)

View related products by pathway.

PTMs for Lgr5/GPR49 Antibody (NBP1-28904)

Learn more about PTMs related to Lgr5/GPR49 Antibody (NBP1-28904).
 

Research Areas for Lgr5/GPR49 Antibody (NBP1-28904)

Find related products by research area.

Blogs on Lgr5/GPR49.

Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Kwonil Jung
01/14/2014
Application: IHC-Fr
Species: Other

Bioinformatics

Gene Symbol LGR5
Entrez
Uniprot