Lgr5/GPR49 Antibody

Images

 
Immunohistochemistry-Frozen: Lgr5/GPR49 Antibody [NBP1-28904] - Staining on pig small intestine from a review by a confirmed customer.
Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Analysis of human small intestine.

Product Details

Summary
Reactivity Hu, PoSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P, ICC/IF (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Lgr5/GPR49 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of the human GPR49/LGR5 protein (within residues 650-700). [Swiss-Prot# O75473]
Localization
Cell-membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LGR5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0-2.0 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This GPR49/LGR5 antibody is useful for Immunohistochemistry on paraffin-embedded sections and Western blot. This antibody is not recommended for Immunocytochemistry/Immunofluorescence.
Control Peptide
Lgr5/GPR49 Peptide (NBP1-28904PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-28904 in the following applications:

Publications
Read Publications using
NBP1-28904 in the following applications:

Reactivity Notes

Human and pig.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lgr5/GPR49 Antibody

  • FEX
  • G protein-coupled receptor 49
  • GPR49
  • GPR49G-protein coupled receptor HG38
  • GPR67
  • G-protein coupled receptor 49
  • G-protein coupled receptor 67
  • GRP49
  • HG38
  • leucine-rich repeat containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G-protein coupled receptor 5
  • Lgr5
  • MGC117008
  • orphan G protein-coupled receptor HG38

Background

GPR49/LGR5 (G protein-coupled receptor 49 / leucine-rich repeat-containing G protein-coupled receptor 5), also called FEX, GPR67, GRP49, HG38 or MGC117008), is structurally related to members of glycoprotein hormone receptor family including FSH-R, TSH-R and LH-R. LGRs (leucine-rich repeat-containing G-protein coupled receptor) are divided into three major subgroups: glycoprotein hormone receptors; the subgroup of GPR48, GPR49, and LGR6; and receptors of relaxin family ligands (LGR7 and LGR8), of which GPR49 is emerging as a significant player in stem cells biology as it is highly expressed on stem cells in small intestine, colon and hair follicle. Deficiency of GPR49 in mice has been shown to cause neonatal lethality characterized by ankyloglossia, gastrointestinal distension and induction of premature differentiation of Paneth cells. LGR5 is a downstream target gene of Wnt signaling pathway (critical for morphogenesis and tumorigenesis) and it has also been reported as a receptor for R-spondins which are potent mediators of Wnt/beta-catenin as well as Wnt/PCP signaling. Accordingly, when compared to normal tissues, LGR5 is found upregulated in certain cancers e.g liver cancer with beta-catenin mutations, basal cell carcinoma, colon and ovarian carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lgr5/GPR49 Antibody (NBP1-28904)(3)

We have publications tested in 2 confirmed species: Human, Porcine.

We have publications tested in 2 applications: IHC-Fr, IHC-P.


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IHC-Fr
(1)
IHC-P
(1)
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Human
(1)
Porcine
(1)
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Review for Lgr5/GPR49 Antibody (NBP1-28904) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-28904:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Other
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Lgr5/GPR49 NBP1-28904
Enlarge
reviewed by:
Kwonil Jung
IHC-Fr Other 01/14/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedPig small intestine
SpeciesOther

Blocking

Blocking Details1x Powerblocking solution (Casein) in distilled water (Biogenex) for 30 mins at room temp

Primary Anitbody

Dilution Ratio1:30 in PBS including 0.1% tween 20 overnight

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, Alexa Fluor 594
Secondary Manufacturer Cat#A-11012 Invitrogen
Secondary Concentration1:200 in PBS

Details

Detection NotesFluorescence microscpy examination
Fixation DetailsFrozen tissues are fixed in paraforaldehyde buffer for 20 min, without antigen retrieval step.
Wash DescriptionVigorous wash with PBS

Product General Protocols

View specific protocols for Lgr5/GPR49 Antibody (NBP1-28904): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Lgr5/GPR49 Antibody (NBP1-28904). (Showing 1 - 3 of 3 FAQs).

  1. This morning I have a customer who has purchased NBP1-28904 and would like to test it in flow cytometry. She is new to flow and is wondering if you might have a recommended protocol. Also, I see that the immunogen is within aa 650-700 which appears to span both helical and cytoplasmic domains. Do you know if the exact immunogen is fully within one domain or the other? Is permeabilization required? Any information you have is much appreciated.
    • Unfortunately I cannot disclose anything more about the immunogen sequence; however, our lab does recommend permeabilization for using this antibody. This is our general flow cytometry protocol. Note that if she tests this antibody in flow, she would be eligible for our Innovators Reward Program.
  2. Is it possible to confirm that the actual immunogen is taken from within the actual cytoplasmic domain (aa 660-682)?
    • Yes, I can confirm that the immunogen falls within the cytoplasmic domain of this protein.
  3. Please inform us this item NBP1-28904 is stain cytoplasmic or stain Nuclus?
    • NBP1-28904 detects Lgr5/GPR49 protein which localize to Cell membrane and the Golgi apparatus. Therefore, this antibody should generate a staining pattern with more signal in membranes and in cytoplasm as patches around the nucleus of the cells. More information on this protein may be found at Uniprot site

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-28904AF405
Alexa Fluor 488 Labeled NBP1-28904AF488
Alexa Fluor 647 Labeled NBP1-28904AF647
Alexa Fluor 700 Labeled NBP1-28904AF700
Biotin Labeled NBP1-28904B
DyLight 405 Labeled NBP1-28904V
DyLight 488 Labeled NBP1-28904G
DyLight 550 Labeled NBP1-28904R
DyLight 650 Labeled NBP1-28904C
FITC Labeled NBP1-28904F
HRP Labeled NBP1-28904H

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Bioinformatics Tool for Lgr5/GPR49 Antibody (NBP1-28904)

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Recent Reviews

4
5
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4
1
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0
2
0
1
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Kwonil Jung
01/14/2014
Application: IHC-Fr
Species: Other

Bioinformatics

Gene Symbol LGR5
Entrez
Uniprot