AKT1 [p Ser473] Antibody (104A282) (NB100-56749)

Images

 
Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Akt1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/ml) for the indicated times was ...read more
Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:250 dilution of pSer473 AKT1 ...read more
Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Akt1 [p Ser473] Antibody (104A282) [NB100-56749] - Analysis using Azide/BSA FREE version of NB100-56749. Analysis of phospho AKT using phospho AKT ...read more
Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:250 dilution of pSer473 AKT1 ...read more

Product Details

Summary
Reactivity Hu, Mu, Am, Ch, ZeSpecies Glossary
Applications WB, IHC, IHC-P
Clone
104A282
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

AKT1 [p Ser473] Antibody (104A282) Summary

Immunogen
This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 473 of human Akt1.
Modification
p Ser473
Specificity
Clone 104A282 detects specifically the Ser 473 Phosphorylated form of AKT.
Predicted Species
Zebrafish (100%), Amphibian (100%), Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AKT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry 1:200 - 1:250
  • Immunohistochemistry-Paraffin 1:200 - 1:250
Reviewed Applications
Read 1 Review rated 5
using
NB100-56749 in the following applications:

Publications
Read Publications using
NB100-56749 in the following applications:

  • WB
    5 publications

Reactivity Notes

Cross reacts with Human and Mouse. Predicted to cross-react with Zebrafish, Frog and Chicken.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for AKT1 [p Ser473] Antibody (104A282)

  • AKT
  • Akt1
  • EC 2.7.11
  • EC 2.7.11.1
  • PKB alpha
  • PKBMGC99656
  • PRKBA
  • Protein kinase B
  • Proto-oncogene c-Akt
  • rac protein kinase alpha
  • RAC-alpha serine/threonine-protein kinase
  • RAC-alpha
  • RAC-PK-alpha
  • RACPKB-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1

Background

Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. Akt/PKB phosphorylation is critical for it activity. The major phosphorylation sites required for Akt activation has been identified as threoinine 308 and serine 473. Serine 473 is phosphorylated by MAPKAP kinase 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-46403
Species: Hu
Applications: IHC
NBP2-15071
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-33116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-46404
Species: Hu
Applications: IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-91228
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-240
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IP, PLA
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
AF1095
Species: Hu
Applications: WB, Simple Western, IHC, CyTOF-ready, ICC, ICFlow
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
AF847
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
AF3978
Species: Hu, Mu, Rt
Applications: WB, IHC
Supplier Logo

Publications for AKT1 Antibody (NB100-56749)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(5)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-56749 Applications Species
Meads MB, Fang B, Mathews L et al. Targeting PYK2 mediates microenvironment-specific cell death in multiple myeloma. Oncogene 2015 Sep 21 [PMID: 26387544] (WB, Human) WB Human
Tagoug Ines, Sauty De Chalon Amelie, Dumontet Charles. Inhibition of IGF-1 signalling enhances the apoptotic effect of AS602868, an IKK2 inhibitor, in multiple myeloma cell lines. PLoS One. 2011 [PMID: 21799925] (WB, Human) WB Human
Wedel S, Hudak L, Seibel JM et al. Inhibitory effects of the HDAC inhibitor valproic acid on prostate cancer growth are enhanced by simultaneous application of the mTOR inhibitor RAD001. Life Sci. 2011 Feb 28 [PMID: 21192952] (WB)

Details:
Phosphorylated AkT1 Ser473 (IMG-187A). WB: PC-3, DU-145, LNCaP cell lines treated with either RAD001 or VPA, Fig 5.
WB
Wedel S, Hudak L, Seibel JM et al. Critical analysis of simultaneous blockage of histone deacetylase and multiple receptor tyrosine kinase in the treatment of prostate cancer. Prostate. 2011 May 15 [PMID: 20954195] (WB)

Details:
Product cited: AKT1 Ser473 (IMG-187A). WB: PC-3, DU-145, LNCaP cell lines treated with either AEE788 or VPA, Fig 8.
WB
Elamin MH, Shinwari Z, Hendrayani SF et al. Curcumin inhibits the Sonic Hedgehog signaling pathway and triggers apoptosis in medulloblastoma cells. Mol Carcinog. 2010 Mar [PMID: 20025076]
Juengel E, Engler J, Natsheh I et al. Combining the receptor tyrosine kinase inhibitor AEE788 and the mammalian target of rapamycin (mTOR) inhibitor RAD001 strongly inhibits adhesion and growth of renal cell carcinoma cells. BMC Cancer. 2009 May 27 [PMID: 19473483] (WB)

Details:
Phosphorylated AkT1 Ser473 (IMG-187A). WB: A498 and Caki-1 cell lines treated with AEE788 or RAD001, Fig 11.
WB
Luty WH, Rodeberg D, Parness J, Vyas YM. Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates. J Immunol. 2007 Jul 15 [PMID: 17617572]
Nair AS, Shishodia S, Ahn KS et al. Deguelin, an Akt inhibitor, suppresses IkappaBalpha kinase activation leading to suppression of NF-kappaB-regulated gene expression, potentiation of apoptosis, and inhibition of cellular invasion. J Immunol. 2006 Oct 15 [PMID: 17015749]

Details:
Suppression of NF-kB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes; Implications for the treatment of osteoarthritics. Shakibaei M, T John, G Schulze-Tanzil, I

Review for AKT1 Antibody (NB100-56749) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-56749:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot AKT1 NB100-56749
Enlarge
reviewed by:
Liyong Zhang
WB Human 08/16/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for AKT1 Antibody (NB100-56749). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for a antibody that recognizes human Akt1 but NOT Akt2 or 3, for Western blot analyses. I also want that antibody to recognize Akt1 regardless of its phosphorylated form.
    • At the moment we do not have an AKT1 antibody that definitively does not react with either AKT2 or AKT3.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56749AF405
Alexa Fluor 488 Labeled NB100-56749AF488
Alexa Fluor 647 Labeled NB100-56749AF647
Alexa Fluor 700 Labeled NB100-56749AF700
DyLight 350 Labeled NB100-56749UV
DyLight 405 Labeled NB100-56749V
DyLight 488 Labeled NB100-56749G
DyLight 550 Labeled NB100-56749R
DyLight 650 Labeled NB100-56749C
DyLight 680 Labeled NB100-56749FR
DyLight 755 Labeled NB100-56749IR
HRP Labeled NB100-56749H

Additional AKT1 [p Ser473] Antibody (104A282) Products

Related Products by Gene

Bioinformatics Tool for AKT1 Antibody (NB100-56749)

Discover related pathways, diseases and genes to AKT1 Antibody (NB100-56749). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for AKT1 Antibody (NB100-56749)

Discover more about diseases related to AKT1 Antibody (NB100-56749).
 

Pathways for AKT1 Antibody (NB100-56749)

View related products by pathway.

PTMs for AKT1 Antibody (NB100-56749)

Learn more about PTMs related to AKT1 Antibody (NB100-56749).
 

Research Areas for AKT1 Antibody (NB100-56749)

Find related products by research area.

Blogs on AKT1. Showing 1-10 of 11 blog posts - Show all blog posts.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

AKT1 - Regulating cell growth and survival through phosphorylation
AKT1 is a serine/threonine protein kinase with homology to protein kinase A (PKA) and protein kinase C (PKC). AKT1 contains the central kinase domain sandwiched between a pleckstrin homology domain and a regulatory domain (1). AKT1 is regulated by...  Read full blog post.

Akt1 - a central player in cell survival signaling
Akt1 is one of three isoforms of Akt belonging to the AGC family of serine/threonine kinases (Akt1, Akt2, and Akt3). All Akt isoforms contain an N-terminal Plekstrin Homology (PH) domain, a C-terminal regulatory domain, and a central catalytic kin...  Read full blog post.

AKT1, Scene 1: The Cell Must Go On
Akt1 is a serine/threonine-specific protein kinase involved in many cellular signaling pathways. The major function of this kinase is to mediate cell survival, but it also plays key roles in various other cellular functions such as glycogen synthesis...  Read full blog post.

Caspase 9 and Mitochondrial Apoptosis Regulation
Caspase 9 (also termed ICE-LAP6, Mch6, Apaf-3) is a member of cysteine protease family of caspases and is encoded by the CASP9 gene in humans. Caspase-9 is involved in mitochondrial apoptosis pathway and is an initiator caspase. Pro-caspase-9 is activ...  Read full blog post.

Mapping Signal Transduction with mTOR Antibodies
The protein encoded by mTOR (mammalian target of rapamycin), also known as dTOR in Drosophila, belongs to a family of phosphatidylinositol kinase-related kinases. These kinases regulate fundamental processes of cell growth, proliferation, metabolism...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Antibodies Targeting the mTOR Pathway for Many Diseases
The mammalian target of rapamycin (mTOR) is a downstream effector of the phosphatidylinositol 3-kinase (PI3K)/Akt (protein kinase B) signaling pathway that mediates cell survival and proliferation. It is increasingly apparent that mTOR signaling impac...  Read full blog post.

AKT Antibody Assays: A Complex Area with an Easy Solution
We at Novus Biologicals place a lot of emphasis on the kinase signaling pathways. Kinases, or phosphotransferase enzymes play a key role in phosphorylation signaling. Over 500 human protein kinases have so far been discovered. They play essential role...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Liyong Zhang
08/16/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol AKT1
Entrez
Uniprot

Customers Who Bought This Also Bought