ZP2 Antibody


Western Blot: ZP2 Antibody [NBP2-21053] - Sample (30 ug of whole cell lysate) A: IMR32 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: ZP2 Antibody [NBP2-21053] - Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using antibody at 1:250 dilution.

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
1 mg/ml

Order Details

ZP2 Antibody Summary

Recombinant fragment corresponding to a region within amino acids 1 and 313 of ZP2 (Uniprot ID#Q05996)
Processed zona pellucida sperm-binding protein 2: Secreted; extracellular space; extracellular matrix; Cell membrane; Single-pass type I membrane protein
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
1XPBS, 1%BSA, 20% Glycerol (pH7).
0.01% Thimerosal
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
82 kDa

Alternate Names for ZP2 Antibody

  • AFP regulator 1
  • AFR1
  • Alpha-fetoprotein regulator 1
  • KIAA0854RAF
  • Regulator of AFP
  • transcription factor ZHX2
  • Zinc finger and homeodomain protein 2
  • zinc fingers and homeoboxes 2
  • zinc fingers and homeoboxes protein 2
  • zinc-fingers and homeoboxes 2


The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. [provided by RefSeq]


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol ZP2

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