Patched 1/PTCH Antibody - BSA Free

Images

 
Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ...read more
Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Evaluation of IGF-1, IGF1R and HH pathway expression in fibroblasts and oral squamous carcinoma cells. IGF1 mRNA expression in ...read more
Immunohistochemistry: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Increased Ihh mRNA and protein levels and activity in Smad2 and Smad3 mutant growth plates.All images are P0 proximal tibias. Immunostaining for ...read more
Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Analysis of Human PTCH1 in Fetal brain Lysate with anti huPTCH1 at a 1:500 dilution.
Increased Ihh mRNA and protein levels and activity in Smad2 and Smad3 mutant growth plates.All images are P0 proximal tibias. (D) Immunostaining for Ptch1, showing increased protein levels throughout the growth plate in ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Patched 1/PTCH Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to residues 269-279 of the human and mouse PTCH protein.
Specificity
Patched 1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PTCH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:1000
Application Notes
This antibody may be used for Western blot analysis where a band is seen at ~161 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Patched 1/PTCH Overexpression Lysate
Publications
Read Publications using
NB200-118 in the following applications:

Reactivity Notes

Reacts with human, mouse, and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Patched 1/PTCH Antibody - BSA Free

  • BCNS
  • BCNSFLJ42602
  • FLJ26746
  • HPE7
  • mes
  • NBCCS
  • patched (Drosophila) homolog
  • Patched 1
  • patched homolog (Drosophila)
  • patched homolog 1 (Drosophila)
  • patched homolog 1
  • protein patched homolog 1
  • PTC
  • ptc1
  • PTCH protein +12b
  • PTCH protein +4'
  • PTCH protein -10
  • PTCH
  • PTCH1
  • PTCH11

Background

Mutations of the human Patched tumor suppressor gene (PTCH) have been identified in individuals with the nevoid basal cell carcinoma syndrome (NBCCS) as well as in sporadic basal cell carcinomas and medulloblastomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Patched 1/PTCH Antibody (NB200-118)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IF/IHC
(3)
IHC-Fr
(1)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
Rat
(3)
All Species
Showing Publications 1 - 6 of 6.
Publications using NB200-118 Applications Species
Ferreira Mendes, J M, de Faro Valverde, L Et al. Effects of IGF-1 on Proliferation, Angiogenesis, Tumor Stem Cell Populations and Activation of AKT and Hedgehog Pathways in Oral Squamous Cell Carcinoma. Int J Mol Sci 2020-09-05 [PMID: 32899449] (IF/IHC, Rat) IF/IHC Rat
Bacelar Sacramento de AraUjo T, de Oliveira Siquara da Rocha L, Torres Andion Vidal M et al. GANT61 Reduces Hedgehog Molecule (GLI1) Expression and Promotes Apoptosis in Metastatic Oral Squamous Cell Carcinoma Cells International journal of molecular sciences 2020-08-24 [PMID: 32846867] (WB, ICC/IF, Human) WB, ICC/IF Human
Gonzalez A C, Santos E T et al. Participation of the Immune System and Hedgehog Signaling in Neoangiogenesis Under Laser Photobiomodulation. J Lasers Med Sci 2019-12-26 [PMID: 31875124] (IF/IHC, Rat) IF/IHC Rat
Wang W, Song B, Anbarchian T et al. Smad2 and Smad3 Regulate Chondrocyte Proliferation and Differentiation in the Growth Plate. PLoS Genet. 2016-10-01 [PMID: 27741240] (IF/IHC, Mouse) IF/IHC Mouse
Martinez JA, Kobayashi M, Krishnan A et al. Intrinsic facilitation of adult peripheral nerve regeneration by the Sonic hedgehog morphogen Exp. Neurol. 2015-07-22 [PMID: 26210874] (IHC-Fr, Rat) IHC-Fr Rat
Kaminagakura E, Almeida JD, Carvalho YR et al. Keratocyst of the buccal mucosa: case report and immunohistochemical comparative study with sporadic intraosseous keratocystic odontogenic tumor. Oral Surg Oral Med Oral Pathol Oral Radiol 2013-05-10 [PMID: 23669203] (IHC-P, Human) IHC-P Human

Reviews for Patched 1/PTCH Antibody (NB200-118) (0)

There are no reviews for Patched 1/PTCH Antibody (NB200-118). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Patched 1/PTCH Antibody (NB200-118). (Showing 1 - 3 of 3 FAQs).

  1. I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?
    • NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.
  2. Could you please tell me an alternative for NBP1-52497?
    • An appropriate replacement would be NB200-118.
  3. Does this antibody detect an epitope within the first extracellular loop of Ptch1? Is the residue 269-279 within this loop?
    • According to the UniProt entry (http://www.uniprot.org/uniprot/Q13635), the first extracellular loop corresponds to amino acids 122-436, so yes, the immunogen/epitope is within this region.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Patched 1/PTCH Products

Research Areas for Patched 1/PTCH Antibody (NB200-118)

Find related products by research area.

Blogs on Patched 1/PTCH

There are no specific blogs for Patched 1/PTCH, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Patched 1/PTCH Antibody - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol PTCH1
Entrez