HAND2 Antibody [Unconjugated]

HAND2 was detected in immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo using Goat Anti-Human/Mouse HAND2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3876) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei in developing tongue. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

HAND2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human HAND2
  Met1-Gln217
  Accession # P61296
• Specificity: Detects human and mouse HAND2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human HAND1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HAND2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HAND2 Antibody [Unconjugated]

• BHLHA26
• bHLHa26MGC125303
• dHand
• DHAND2
• Ehand2
• FLJ16260
• HAND2
• heart and neural crest derivatives expressed 2
• heart, autonomic nervous system and neural crestderivatives-expressed protein 2
• Hed
• MGC125304
• Th2
• Thing2

Background

HAND2 (also known as dHAND, Thing-2 and Hed) is a 24 kDa, class B member of the basic helix-loop-helix transcription factors superfamily. HAND2 is essential for cardiac morphogenesis and required for vascular development and regulation of angiogenesis, possibly through VEGF signaling. HAND2 is also implicated in limb development acting as an upstream regulator of sonic hedgehog. Alternative initiation and internal deletions result in at least three potential HAND2 isoforms. Human and mouse HAND2 proteins are 100% identical.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HAND2 Antibody (AF3876)(2)

Publications using AF3876

• H Hashimoto, Z Wang, GA Garry, VS Malladi, GA Botten, W Ye, H Zhou, M Osterwalde, DE Dickel, A Visel, N Liu, R Bassel-Dub, EN Olson Cardiac Reprogramming Factors Synergistically Activate Genome-wide Cardiogenic Stage-Specific Enhancers Cell Stem Cell, 2019-05-09;0(0):. 2019-05-09 [PMID: 31080136] (Western Blot, Human)
• Clementi C, Tripurani S, Large M, Edson M, Creighton C, Hawkins S, Kovanci E, Kaartinen V, Lydon J, Pangas S, DeMayo F, Matzuk M Activin-like kinase 2 functions in peri-implantation uterine signaling in mice and humans. PLoS Genet, 2013-11-14;9(11):e1003863. 2013-11-14 [PMID: 24244176] (IHC, Mouse)

Bioinformatics

• Gene Symbol: HAND2
• Uniprot:
  • P61296()