Furin Antibody

Images

 
Western Blot: Furin Antibody [NB100-1903] - Analysis was performed on membrane enriched extracts (30 ug lysate) of HeLa (Lane 1), HEK 293 (Lane 2), K-562 (Lane 3), SH-SY5Y (Lane 4) and 10uL conditioned media from HeLa ...read more
Immunocytochemistry/ Immunofluorescence: Furin Antibody [NB100-1903] - Immunolocalization of endogenous furin in mouse 3T3 cells.
Flow Cytometry: Furin Antibody [NB100-1903] - Flow cytometry analysis of Furin Convertase was done on HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Ha, PmSpecies Glossary
Applications WB, B/N, Flow, ICC/IF, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2 mg/ml

Order Details

Furin Antibody Summary

Immunogen
Synthetic peptide corresponding to residues R(780) G E R T A F I K D Q S A L(793) of human Furin.
Epitope
Amino acids 781-794.
Marker
TGN Marker
Specificity
Detects Furin convertase from canine and mouse cells as well as transfected human Furin. This does not detect endogenous Furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissues.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FURIN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Blocking/Neutralizing 50 - 200 molar excess
  • Flow Cytometry 3 - 5 ug
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:100
Application Notes
Blocking usage was reported in scientific literature (PMID: 11741999).
Positive Control
Furin Lysate (NBL1-10860)
Reviewed Applications
Read 1 Review rated 5
using
NB100-1903 in the following applications:

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 16030016). Porcine reactivity reported in scientific literature (PMID: 14581457). Rat reactivity reported in scientific literature (PMID: 11696560). Primate reactivity reported in scientific literature (PMID: 11799113).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Furin Antibody

  • Dibasic-processing enzyme
  • EC 3.4.21
  • EC 3.4.21.75
  • FUR
  • FURdibasic processing enzyme
  • furin (paired basic amino acid cleaving enzyme)
  • Furin
  • furin, membrane associated receptor protein
  • PACE
  • PACEFES upstream region
  • paired basic amino acid cleaving enzyme (furin, membrane associated receptorprotein)
  • Paired basic amino acid residue-cleaving enzyme
  • PC
  • PCSK3
  • PCSK3furin
  • proprotein convertase subtilisin/kexin type 3
  • SPC1

Background

Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of Furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Furin Antibody (NB100-1903) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Equine.
We have 1 review tested in 1 application: ICC.

Reviews using NB100-1903:
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ICC
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Furin NB100-1903
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reviewed by:
Anonymous
ICC Equine 05/13/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedPlacental tissue
SpeciesEquine

Comments

Comments1:200 dilution. Heat based antigen retrieval using EDTA based solution. No staining on negative when the primary antibody was omitted.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Anonymous
05/13/2017
Application: ICC
Species: Equine

Bioinformatics

Gene Symbol FURIN
Entrez