Furin Antibody

Images

 
Western Blot: Furin Antibody [NB100-1903] - Analysis was performed on membrane enriched extracts (30 ug lysate) of HeLa (Lane 1), HEK 293 (Lane 2), K-562 (Lane 3), SH-SY5Y (Lane 4) and 10uL conditioned media from HeLa ...read more
Immunocytochemistry/ Immunofluorescence: Furin Antibody [NB100-1903] - Immunolocalization of endogenous furin in mouse 3T3 cells.
Flow Cytometry: Furin Antibody [NB100-1903] - Flow cytometry analysis of Furin Convertase was done on HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Ha, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, B/N
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2 mg/ml

Order Details

Furin Antibody Summary

Immunogen
Synthetic peptide corresponding to residues R(780) G E R T A F I K D Q S A L(793) of human Furin.
Epitope
Amino acids 781-794.
Marker
TGN Marker
Specificity
Detects Furin convertase from canine and mouse cells as well as transfected human Furin. This does not detect endogenous Furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissues.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FURIN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 3 - 5 ug
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:100
  • Block/Neutralize
Application Notes
Blocking usage was reported in scientific literature (PMID: 11741999).
Control
Furin Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-1903 in the following applications:

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 16030016). Porcine reactivity reported in scientific literature (PMID: 14581457). Rat reactivity reported in scientific literature (PMID: 11696560). Primate reactivity reported in scientific literature (PMID: 11799113).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Furin Antibody

  • Dibasic-processing enzyme
  • EC 3.4.21
  • EC 3.4.21.75
  • FUR
  • FURdibasic processing enzyme
  • furin (paired basic amino acid cleaving enzyme)
  • Furin
  • furin, membrane associated receptor protein
  • PACE
  • PACEFES upstream region
  • paired basic amino acid cleaving enzyme (furin, membrane associated receptorprotein)
  • Paired basic amino acid residue-cleaving enzyme
  • PC
  • PCSK3
  • PCSK3furin
  • proprotein convertase subtilisin/kexin type 3
  • SPC1

Background

Furin (also known as FUR, PACE, PCSK3, and SCP1) is a ubiquity expressed, calcium-dependent serine protease that belongs to the subtilisin-like proprotein convertase (PC) family (1). Furin is synthesized as a 794-amino acid type-I transmembrane protein (1). Like other PC family members, furin shares structural similarity which includes a heterogeneous ~10 kDa amino terminal proregion, a ~55 kDa highly conserved subtilisin-like catalytic domain, and a carboxylterminal domain that is heterogeneous and varies in length and sequence between different PC family members (2). Furin plays an important role in embryogenesis and assists in the maturation of proprotein substrates (1,3). As a protease, furin cleaves and activates over 150 mammalian, viral, and bacterial substrates (3). These substrates include growth factors, receptors, hormones, cytokines, and adhesion molecules (1, 3). Furin processes these proproteins in secretory pathway compartments by cleaving at the carboxyl-terminus of the consensus sequence RX(K/R)R (where X is any amino acid) (1). Furin is localized to the trans-Golgi-network by the acidic peptide sequence C771PSDSEEDEG780 where phosphorylation of the serine residues regulates the intracellular trafficking. Another unique signaling domain is the hydrophobic motif Y759KGL762 that modulates endocytosis from the cell surface. The theoretical molecular weight of furin is 87 kDa; however, the observed molecular weight may vary as post-translational modifications, like glycosylation, cause the pre-pro furin to run at 110-104 kDa, the mature furin at 98-95 kDa, and the shed furin at 90 kDa (4).

Proteolytic cleavage regulates several physiological processes in both health and disease (3). Abnormal activity or mutations in proteases, including furin, is associated with pathologies and diseases including cancer, cardiovascular disorders, diabetes, inflammation, neurological diseases, and autoimmune diseases (3). As mentioned above, furin also acts upon bacterial substrates, including anthrax and Shiga toxin, and many virus families such as Herpes-, Flavi-, and Corona-, leading to host infections. Furthermore, the novel coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) present with a S-spike protein that is cleaved by PCs, including furin, at the S1/S2 cleavage site (5, 6). The cleavage allows the SARS-CoV-2 to then attach to the angiotensin-converting enzyme 2 (ACE2) receptor via the S1 domain and the cellular membrane via the S2 domain (5, 6). Although COVID-19 patients mostly present with respiratory symptoms, a variety of other systems are affected including cardiovascular, gastrointestinal (GI), and the liver (5-7). It is suggested that the S1/furin/ACE2 interaction promotes SARS-CoV-2 infection leading to the harmful symptoms and reactions in patients (5, 6). Cardiovascular disease is a common comorbidity in patients, along with hypertension, myocardial damage, and heart palpitations (Ming). Further evidence of furin being a risk factor for infection is the high levels of furin present in the blood of heart failure patients (5). Similarly, the small bowel may be another interaction site for infection as it is rich in furin and the intestinal enterocytes have many ACE2 receptors (6). Furin is also highly expressed in the liver and hepatocytes and cholangiocytes of the liver present ACE2 receptors (3, 7). Studies have shown that up one-third of COVID-19 patients experience GI symptoms which range from diarrhea and loss of appetite to abdominal cramping and bloody stool (6, 7). Additionally, some patients displayed abnormal liver enzyme levels (7). It has been suggested that a possible therapeutic strategy for treating those infected with SARS-CoV-2 is pharmacologically or immunologically modulating furin or ACE2 binding sites to combat COVID-19 infection (3, 5).

References

1. Thomas, G. (2002). Furin at the cutting edge: from protein traffic to emryogenesis and disease. Nature Rev. Mol. Cell Biol. https://doi.org/10.1038/nrm934

2. Zhou A., Paquet, L., & Mains, R.E. (1995). Structural elements that direct specific processing of different mammalian subtilisin-like prohormone convertases. J Biol Chem. https://doi.org/10.1074/jbc.270.37.21509

3. Braun E., & Sauter, D. (2019). Furin-mediated protein processing in infectious diseases and cancer. Clin Transl Immunology. https://doi:10.1002/cti2.1073

4. Atlas of Genetics and Cytogenetics in Oncology and Haematology, FURIN

5. Ming, Y. & Qiang, L. (2020). Involvement of Spike Protein, Furin, and ACE2 in SARS-CoV-2-Related Cardiovascular Complications. SN Compr. Clin. Med. https://doi.org/10.1007/s42399-020-00400-2

6. Monkemuller, K., Fry, L., & Rickes, S. (2020). COVID-19, coronavirus, SARS-CoV-2 and the small bowel. Rev Esp Enferm Dig. https://doi:10.17235/reed.2020.7137/2020

7. Agarwal, A., Chen, A., Ravindran, N., To, C., & Thuluvath, P.J. (2020). Gastrointestinal and Liver Manifestations of COVID-19. J Clin Exp Hepatol. https://doi:10.1016/j.jceh.2020.03.001

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for Furin Antibody (NB100-1903) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Equine.

Reviews using NB100-1903:
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Images Ratings Applications Species Date Details
Immunocytochemistry Furin NB100-1903
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5
reviewed by:
Anonymous
ICC Equine 05/13/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedPlacental tissue
SpeciesEquine

Comments

Comments1:200 dilution. Heat based antigen retrieval using EDTA based solution. No staining on negative when the primary antibody was omitted.

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Recent Reviews

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Anonymous
05/13/2017
Application: ICC
Species: Equine

Bioinformatics

Gene Symbol FURIN
Entrez