Recombinant Human/Mouse FGF-8b Protein

Recombinant Human/Mouse FGF-8b (Catalog # 423-F8) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 6.5-40 ng/mL in the presence of 1 μg/mL heparin.

1 μg/lane of Recombinant Human/Mouse FGF-8b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.

• Reactivity: Hu, Mu
• Applications: Bioactivity

Recombinant Human/Mouse FGF-8b Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is typically 6.5-40 ng/mL in the presence of 1 µg/mL heparin.
• Source: E. coli-derived FGF-8 protein
  Gln23-Arg215, with an N-terminal Met
• Accession #: NP_006110 (human), NP_001159834 (mouse)
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: FGF8
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 22.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 23 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄ and Brij-35 with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse FGF-8b Protein

• AIGF
• AIGFKAL6
• Androgen-induced growth factor
• FGF8
• FGF-8
• fibroblast growth factor 8 (androgen-induced)
• fibroblast growth factor 8
• HBGF-8
• Heparin-binding growth factor 8
• MGC149376

Background

FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1-3). Alternate splicing of mouse FGF-8 mRNA generates eight secreted isoforms, designated a-h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5 - 8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9-12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).

1. Mattila, M.M. and P.L. Harkonen (2007) Cytokine Growth Factor Rev. 18:257.
2. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
3. Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928.
4. Gemel, J. et al. (1996) Genomics 35:253.
5. Olsen, S.K. et al. (2006) Genes Dev. 20:185.
6. Crossley, P.H. et al. (1996) Cell, 84:127.
7. Heikinheimo, M. et al. (1994) Mech. Dev. 48:129.
8. Sun, X. et al. (1999) Genes Dev. 13:1834.
9. Ghosh, A.K. et al. (1996) Cell Growth Differ. 7:1425.
10. Mattila, M.M. et al. (2001) Oncogene 20:2791.
11. Valve, E. et al. (2000) Int. J. Cancer 88:718.
12. Valve, E.M. et al. (2001) Lab. Invest. 81:815.
13. Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.

Publications for FGF-8 (423-F8)(39)

Publications using 423-F8

• G Collo, L Cavalleri, M Zoli, U Maskos, E Ratti, E Merlo Pich Alpha6-Containing Nicotinic Acetylcholine Receptors Mediate Nicotine-Induced Structural Plasticity in Mouse and Human iPSC-Derived Dopaminergic Neurons Front Pharmacol, 2018;9(0):572. 2018 [PMID: 29910731] (Bioassay, Human)
• J Li, F Xing, F Chen, L He, KF So, Y Liu, J Xiao Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages Cell Transplant, 2018;0(0):9636897187803. 2018 [PMID: 29895168] (Bioassay, Human)
• X Hu, JW Lee, X Zheng, J Zhang, X Lin, Y Song, B Wang, X Hu, HH Chang, Y Chen, CP Lin, Y Zhang Efficient induction of functional ameloblasts from human keratinocyte stem cells Stem Cell Res Ther, 2018;9(1):126. 2018 [PMID: 29720250] (Bioassay, Human)
• G Shall, M Menosky, S Decker, P Nethala, R Welchko, X Leveque, M Lu, M Sandstrom, U Hochgeschw, J Rossignol, G Dunbar Effects of Passage Number and Differentiation Protocol on the Generation of Dopaminergic Neurons from Rat Bone Marrow-Derived Mesenchymal Stem Cells Int J Mol Sci, 2018;19(3):. 2018 [PMID: 29498713] (Bioassay, Rat)
• T Johansen, C Krabbe, SI Schmidt, AM Serrano, M Meyer Comparative Analysis of Spontaneous and Stimulus-Evoked Calcium Transients in Proliferating and Differentiating Human Midbrain-Derived Stem Cells Stem Cells Int, 2017;2017(0):9605432. 2017 [PMID: 29201062] (Bioassay, Human)
• AR Rodrigues, N Yakushiji-, Y Atsuta, G Andrey, P Schorderet, D Duboule, CJ Tabin Integration of Shh and Fgf signaling in controlling Hox gene expression in cultured limb cells Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28270602] (Bioassay, Chicken)
• SA Liddelow, KA Guttenplan, LE Clarke, FC Bennett, CJ Bohlen, L Schirmer, ML Bennett, AE Mnch, WS Chung, TC Peterson, DK Wilton, A Frouin, BA Napier, N Panicker, M Kumar, MS Buckwalter, DH Rowitch, VL Dawson, TM Dawson, B Stevens, BA Barres Neurotoxic reactive astrocytes are induced by activated microglia Nature, 2017;541(7638):481-487. 2017 [PMID: 28099414] (Bioassay, Human)
• Florian Wegner Neuronal Dysfunction in iPSC-Derived Medium Spiny Neurons from Chorea-Acanthocytosis Patients Is Reversed by Src Kinase Inhibition and F-Actin Stabilization J. Neurosci., 2016;36(47):12027-12043. 2016 [PMID: 27881786] (Bioassay, Human)
• Fibulin-1 Binds to Fibroblast Growth Factor 8 with High Affinity: Effects on Embryo Survival J Biol Chem, 2016;0(0):. 2016 [PMID: 27402846] (Bioassay, Human)
• Daniel A Monti N-Acetyl Cysteine May Support Dopamine Neurons in Parkinson&#039;s Disease: Preliminary Clinical and Cell Line Data PLoS ONE, 2016;11(6):e0157602. 2016 [PMID: 27309537] (Bioassay, Human)
• Eva Hedlund Dopamine Receptor Antagonists Enhance Proliferation and Neurogenesis of Midbrain Lmx1a-expressing Progenitors Sci Rep, 2016;6(0):26448. 2016 [PMID: 27246266] (Bioassay, Mouse)
• Chan W, Howe K, Clegg J, Guimond S, Price D, Turnbull J, Pratt T 2-O Heparan Sulfate Sulfation by Hs2st Is Required for Erk/Mapk Signalling Activation at the Mid-Gestational Mouse Telencephalic Midline. PLoS ONE, 2015;10(6):e0130147. 2015 [PMID: 26075383] (Bioassay, Mouse)
• Shen, Shih-Che, Shen, Ching-I, Lin, Ho, Chen, Chun-Jun, Chang, Chia-Yu, Chen, Sheng-Me, Lee, Hsiu-Chi, Lai, Ping-Sha, Su, Hong-Lin Susceptibility of human embryonic stem cell-derived neural cells to Japanese encephalitis virus infection. PLoS ONE, 2014;9(12):e114990. 2014 [PMID: 25517725] (Bioassay, Human)
• Attia L, Schneider J, Yelin R, Schultheiss T Collective cell migration of the nephric duct requires FGF signaling. Dev Dyn, 2015;244(2):157-67. 2015 [PMID: 25516335] (Bioassay, Chicken)
• Yamamoto-Shiraishi Y, Higuchi H, Yamamoto S, Hirano M, Kuroiwa A Etv1 and Ewsr1 cooperatively regulate limb mesenchymal Fgf10 expression in response to apical ectodermal ridge-derived fibroblast growth factor signal. Dev Biol, 2014;394(1):181-90. 2014 [PMID: 25109552] (Bioassay, Chicken)
• Lewandowski J, Pursell T, Rabinowitz A, Vokes S Manipulating gene expression and signaling activity in cultured mouse limb bud cells. Dev Dyn, 2014;243(7):928-36. 2014 [PMID: 24633820] (Bioassay, Mouse)
• Mong J, Panman L, Alekseenko Z, Kee N, Stanton L, Ericson J, Perlmann T Transcription factor-induced lineage programming of noradrenaline and motor neurons from embryonic stem cells. Stem Cells, 2014;32(3):609-22. 2014 [PMID: 24549637] (Bioassay, Mouse)
• Ni, Na, Hu, Yaohua, Ren, Huixia, Luo, Chuanmin, Li, Peng, Wan, Jian-Bo, Su, Huanxing Self-assembling peptide nanofiber scaffolds enhance dopaminergic differentiation of mouse pluripotent stem cells in 3-dimensional culture. PLoS ONE, 2013;8(12):e84504. 2013 [PMID: 24376815] (Bioassay, Mouse)
• McCarroll, Matthew, Nechiporuk, Alex V Fgf3 and Fgf10a work in concert to promote maturation of the epibranchial placodes in zebrafish. PLoS ONE, 2013;8(12):e85087. 2013 [PMID: 24358375] (In Vivo, Zebrafish)
• Stappert L, Borghese L, Roese-Koerner B, Weinhold S, Koch P, Terstegge S, Uhrberg M, Wernet P, Brustle O MicroRNA-based promotion of human neuronal differentiation and subtype specification. PLoS ONE, 2013;8(3):e59011. 2013 [PMID: 23527072] (Bioassay, Human)
• Tomlinson DC, Knowles MA Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancer. Am. J. Pathol., 2010;177(5):2379-86. 2010 [PMID: 20889570] (Bioassay, Human)
• Roelandt P, Pauwelyn KA, Sancho-Bru P Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells. PLoS ONE, 2010;5(8):e12101. 2010 [PMID: 20711405] (Expansion/Differentiation, Rat)
• Nimmagadda S, Geetha-Loganathan P, Scaal M, Christ B, Huang R FGFs, Wnts and BMPs mediate induction of VEGFR-2 (Quek-1) expression during avian somite development. Dev. Biol., 2007;305(2):421-9. 2007 [PMID: 17425953] (In Vivo, Avian - Quail)
• Salero E, Hatten ME Differentiation of ES cells into cerebellar neurons. Proc. Natl. Acad. Sci. U.S.A., 2007;104(8):2997-3002. 2007 [PMID: 17293457] (Bioassay, Mouse)
• Shimizu T, Bae YK, Hibi M Cdx-Hox code controls competence for responding to Fgfs and retinoic acid in zebrafish neural tissue. Development, 2006;133(23):4709-19. 2006 [PMID: 17079270] (Bioassay, Zebrafish)
• Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm. Development, 2006;133(19):3787-96. 2006 [PMID: 16943279] (Bioassay, Mouse)
• Tiecke E, Bangs F, Blaschke R, Farrell ER, Rappold G, Tickle C Expression of the short stature homeobox gene Shox is restricted by proximal and distal signals in chick limb buds and affects the length of skeletal elements. Dev. Biol., 2006;298(2):585-96. 2006 [PMID: 16904661] (In Vivo, Chicken)
• Yamashita H, Nakamura T, Takahashi T, Nagano Y, Hiji M, Hirabayashi T, Amano T, Yagi T, Sakai N, Kohriyama T, Matsumoto M Embryonic stem cell-derived neuron models of Parkinson&apos;s disease exhibit delayed neuronal death. J. Neurochem., 2006;98(1):45-56. 2006 [PMID: 16805795] (Bioassay, Mouse)
• Hutson MR, Zhang P, Stadt HA, Sato AK, Li YX, Burch J, Creazzo TL, Kirby ML Cardiac arterial pole alignment is sensitive to FGF8 signaling in the pharynx. Dev. Biol., 2006;295(2):486-97. 2006 [PMID: 16765936] (Bioassay, Chicken)
• Castelo-Branco G, Sousa KM, Bryja V, Pinto L, Wagner J, Arenas E Ventral midbrain glia express region-specific transcription factors and regulate dopaminergic neurogenesis through Wnt-5a secretion. Mol. Cell. Neurosci., 2005;31(2):251-62. 2005 [PMID: 16243537] (Bioassay, Rat)
• Delfini MC, Dubrulle J, Malapert P, Chal J, Pourquie O Control of the segmentation process by graded MAPK/ERK activation in the chick embryo. Proc. Natl. Acad. Sci. U.S.A., 2005;102(32):11343-8. 2005 [PMID: 16055560] (In Vivo, Chicken)
• Reversade B, Kuroda H, Lee H, Mays A, De Robertis EM Depletion of Bmp2, Bmp4, Bmp7 and Spemann organizer signals induces massive brain formation in Xenopus embryos. Development, 2005;132(15):3381-92. 2005 [PMID: 15975940] (In Vivo, Xenopus)
• Perrier AL, Tabar V, Barberi T, Rubio ME, Bruses J, Topf N, Harrison NL, Studer L Derivation of midbrain dopamine neurons from human embryonic stem cells. Proc. Natl. Acad. Sci. U.S.A., 2004;101(34):12543-8. 2004 [PMID: 15310843] (Bioassay, Human)
• Wiellette EL, Sive H vhnf1 and Fgf signals synergize to specify rhombomere identity in the zebrafish hindbrain. Development, 2003;130(16):3821-9. 2003 [PMID: 12835397] (In Vivo, Zebrafish)
• Gunhaga L, Marklund M, Sjodal M, Hsieh JC, Jessell TM, Edlund T Specification of dorsal telencephalic character by sequential Wnt and FGF signaling. Nat. Neurosci., 2003;6(7):701-7. 2003 [PMID: 12766771] (Bioassay, Chicken)
• Mogi K, Goto M, Ohno E, Azumi Y, Takeuchi S, Toyoizumi R Xenopus neurula left-right asymmetry is respeficied by microinjecting TGF-beta5 protein. Int. J. Dev. Biol., 2003;47(1):15-29. 2003 [PMID: 12653248] (In Vivo, Xenopus)
• Fischer A, Viebahn C, Blum M FGF8 acts as a right determinant during establishment of the left-right axis in the rabbit. Curr. Biol., 2002;12(21):1807-16. 2002 [PMID: 12419180] (Bioassay, Rabbit)
• Abu-Issa R, Smyth G, Smoak I, Yamamura K, Meyers EN Fgf8 is required for pharyngeal arch and cardiovascular development in the mouse. Development, 2002;129(19):4613-25. 2002 [PMID: 12223417] (In Vivo, Mouse)
• Alsan BH, Schultheiss TM Regulation of avian cardiogenesis by Fgf8 signaling. Development, 2002;129(8):1935-43. 2002 [PMID: 11934859] (In Ovo, Chicken)

Bioinformatics

• Gene Symbol: FGF8
• Uniprot:
  • NP_006110 (human), NP_001159834 (mouse)()