Reactivity | Hu, MuSpecies Glossary |
Applications | Bioactivity |
Details of Functionality | Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED 50 for this effect is typically 6.5-40 ng/mL in the presence of 1 µg/mL heparin. |
Source | E. coli-derived FGF-8 protein Gln23-Arg215, with an N-terminal Met |
Accession # | |
N-terminal Sequence | Met |
Protein/Peptide Type | Recombinant Proteins |
Gene | FGF8 |
Purity | >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note | <0.01 EU per 1 μg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 22.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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SDS-PAGE | 23 kDa, reducing conditions |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in MOPS, Na 2 SO 4 and Brij-35 with BSA as a carrier protein. |
Purity | >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions | Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
Stem/Immune cell maintenance or differentiation | 09/11/2019 |
Summary
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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Verified Customer 09/11/2019 |
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Application: | Stem/Immune cell maintenance or differentiation | |
Species: |
Gene Symbol | FGF8 |
Uniprot |