Novus Biologicals products are now on bio-techne.com

Recombinant Human/Mouse FGF-8b Protein

Images

 
Recombinant Human/Mouse FGF-8b (Catalog # 423-F8) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 6.5-40 ng/mL in the presence of 1 μg/mL heparin.
1 μg/lane of Recombinant Human/Mouse FGF-8b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.
Dopaminergic neurons were generated from human pluripotent stem cells in media that included Bovine Fibronectin Protein (1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 ...read more
Dopaminergic neurons were generated from human pluripotent stem cells in media that included Bovine Fibronectin Protein (1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human/Mouse FGF-8b Protein Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is typically 6.5-40 ng/mL in the presence of 1 µg/mL heparin.
Source
E. coli-derived FGF-8 protein
Gln23-Arg215, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
FGF8
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
23 kDa, reducing conditions
Reviewed Applications
Read 1 Review rated 5
using
423-F8 in the following application:

Publications
Read Publications using
423-F8 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and Brij-35 with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse FGF-8b Protein

  • AIGF
  • AIGFKAL6
  • Androgen-induced growth factor
  • FGF8
  • FGF-8
  • fibroblast growth factor 8 (androgen-induced)
  • fibroblast growth factor 8
  • HBGF-8
  • Heparin-binding growth factor 8
  • MGC149376

Background

FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1-3). Alternate splicing of mouse FGF-8 mRNA generates eight secreted isoforms, designated a-h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5 - 8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9-12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).

  1. Mattila, M.M. and P.L. Harkonen (2007) Cytokine Growth Factor Rev. 18:257.
  2. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
  3. Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928.
  4. Gemel, J. et al. (1996) Genomics 35:253.
  5. Olsen, S.K. et al. (2006) Genes Dev. 20:185.
  6. Crossley, P.H. et al. (1996) Cell, 84:127.
  7. Heikinheimo, M. et al. (1994) Mech. Dev. 48:129.
  8. Sun, X. et al. (1999) Genes Dev. 13:1834.
  9. Ghosh, A.K. et al. (1996) Cell Growth Differ. 7:1425.
  10. Mattila, M.M. et al. (2001) Oncogene 20:2791.
  11. Valve, E. et al. (2000) Int. J. Cancer 88:718.
  12. Valve, E.M. et al. (2001) Lab. Invest. 81:815.
  13. Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.

Customers Who Viewed This Item Also Viewed...

314-BP
Species: Hu
Applications: BA, BA
7460-F4/CF
Species: Hu
Applications: BA
233-FB
Species: Hu
Applications: BA
345-FG
Species: Hu
Applications: BA
NB600-1287
Species: Ca, Hu, Mu, Rb, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, WB
H00002263-M01
Species: Bv, Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
1206-F3
Species: Hu
Applications: BA
AF1979
Species: Hu
Applications: ICC, IHC, Simple Western, WB
AF3364
Species: Hu
Applications: ICC, IHC, WB
355-BM
Species: Hu, Mu, Rt
Applications: BA
NBP1-51575
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
AF5045
Species: Hu, Mu
Applications: IHC, WB
AF232
Species: Hu
Applications: IHC, Neut, Simple Western, WB
NB100-55393
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NBP2-20486
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
MAB319
Species: Hu
Applications: IHC, WB
NB300-109
Species: Ch, Dr, Hu, I, Ma, Mu, Rt
Applications: Dual ISH-IHC, ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
H00002019-M04
Species: Hu, Mu
Applications: ELISA, IHC, IHC-P, IP, WB
423-F8
Species: Hu, Mu
Applications: Bioactivity

Publications for FGF-8 (423-F8)(60)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Avian - Quail, Chicken, Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Xenopus, Zebrafish.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, In Ovo, In Vivo.


Filter By Application
Bioassay
(43)
Cell Culture
(6)
Differentiation
(4)
In Ovo
(1)
In Vivo
(8)
All Applications
Filter By Species
Human
(27)
Mouse
(15)
Rat
(4)
Avian - Quail
(1)
Chicken
(8)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Rabbit
(1)
Xenopus
(2)
Zebrafish
(3)
All Species
Showing Publications 1 - 10 of 60. Show All 60 Publications.
Publications using 423-F8 Applications Species
Chen, Y;Kuang, J;Niu, Y;Zhu, H;Chen, X;So, KF;Xu, A;Shi, L; Multiple factors to assist human-derived induced pluripotent stem cells to efficiently differentiate into midbrain dopaminergic neurons Neural regeneration research 2024-04-01 [PMID: 37843228] (Bioassay, Human) Bioassay Human
S Wu, NC Hernandez, DW Sirkis, I Thomas-Wri, R Wade-Marti, R Schekman Unconventional secretion of alpha-synuclein mediated by palmitoylated DNAJC5 oligomers Elife, 2023-01-10;12(0):. 2023-01-10 [PMID: 36626307] (Bioassay, Mouse) Bioassay Mouse
CB Pantazis, A Yang, E Lara, JA McDonough, C Blauwendra, L Peng, H Oguro, J Kanaujiya, J Zou, D Sebesta, G Pratt, E Cross, J Blockwick, P Buxton, L Kinner-Bib, C Medura, C Tompkins, S Hughes, M Santiana, F Faghri, MA Nalls, D Vitale, S Ballard, YA Qi, DM Ramos, KM Anderson, J Stadler, P Narayan, J Papademetr, L Reilly, MP Nelson, S Aggarwal, LU Rosen, P Kirwan, V Pisupati, SL Coon, SW Scholz, T Priebe, M Öttl, J Dong, M Meijer, LJM Janssen, VS Lourenco, R van der Ka, D Crusius, D Paquet, AC Raulin, G Bu, A Held, BJ Wainger, RMC Gabriele, JM Casey, S Wray, D Abu-Bonsra, CL Parish, MS Beccari, DW Cleveland, E Li, IVL Rose, M Kampmann, C Calatayud, P Verstreken, L Heinrich, MY Chen, B Schüle, D Dou, ELF Holzbaur, MC Zanellati, R Basundra, M Deshmukh, S Cohen, R Khanna, M Raman, ZS Nevin, M Matia, J Van Lent, V Timmerman, BR Conklin, K Johnson Ch, K Zhang, S Funes, DA Bosco, L Erlebach, M Welzer, D Kronenberg, G Lyu, E Arenas, E Coccia, L Sarrafha, T Ahfeldt, JC Marioni, WC Skarnes, MR Cookson, ME Ward, FT Merkle A reference human induced pluripotent stem cell line for large-scale collaborative studies Cell Stem Cell, 2022-12-01;29(12):1685-1702.e22. 2022-12-01 [PMID: 36459969] (Bioassay, Human) Bioassay Human
JS Park, J Choi, L Cao, J Mohanty, Y Suzuki, A Park, D Baker, J Schlessing, S Lee Isoform-specific inhibition of FGFR signaling achieved by a de-novo-designed mini-protein Cell Reports, 2022-10-25;41(4):111545. 2022-10-25 [PMID: 36288716] (Bioassay, Human) Bioassay Human
H Ozaki, H Suga, M Sakakibara, M Soen, N Miyake, T Miwata, S Taga, T Nagai, M Kano, K Mitsumoto, T Miyata, T Kobayashi, M Sugiyama, T Onoue, H Takagi, D Hagiwara, S Iwama, R Banno, G Iguchi, Y Takahashi, K Muguruma, H Inoue, H Arima Differentiation of human induced pluripotent stem cells into hypothalamic vasopressin neurons with minimal exogenous signals and partial conversion to the naive state Scientific Reports, 2022-10-17;12(1):17381. 2022-10-17 [PMID: 36253431] (Cell Culture, Human) Cell Culture Human
DS Lee, YJ Song, HR Gug, JH Lee, HS Bae, JC Park Nuclear Factor I-C Regulates Stemness Genes and Proliferation of Stem Cells in Various Mineralized Tissue through Epithelial-Mesenchymal Interactions in Dental Epithelial Stem Cells Stem Cells International, 2022-09-27;2022(0):1092184. 2022-09-27 [PMID: 36213683] (Cell Culture, Mouse) Cell Culture Mouse
L Yu, M Toriseva, S Afshan, M Cangiano, V Fey, A Erickson, H Seikkula, K Alanen, P Taimen, O Ettala, M Nurmi, PJ Boström, M Kallajoki, J Tuomela, T Mirtti, IJ Beumer, M Nees, P Härkönen Increased Expression and Altered Cellular Localization of Fibroblast Growth Factor Receptor-Like 1 (FGFRL1) Are Associated with Prostate Cancer Progression Cancers, 2022-01-07;14(2):. 2022-01-07 [PMID: 35053442] (Bioassay, Human) Bioassay Human
N Hosaka, S Kanda, T Shimono, T Nishiyama Induction of gammadeltaT cells from HSC-enriched BMCs co-cultured with iPSC-derived thymic epithelial cells Journal of Cellular and Molecular Medicine, 2021-10-23;0(0):. 2021-10-23 [PMID: 34687276] (Cell Culture, Mouse) Cell Culture Mouse
M Restan Per, R Sharma, NZ Masri, SM Willerth 3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink Biomolecules, 2021-08-21;11(8):. 2021-08-21 [PMID: 34439916] (Bioassay, Human) Bioassay Human
C Zhou, D Chen, J Ren, D Huang, R Li, H Luo, C Guan, Y Cao, W Wang FGF8 and BMP2 mediated dynamic regulation of dental mesenchyme proliferation and differentiation via Lhx8/Suv39h1 complex Journal of Cellular and Molecular Medicine, 2021-02-13;0(0):. 2021-02-13 [PMID: 33580754] (Bioassay, Human, Mouse) Bioassay Human, Mouse
Show All 60 Publications.

Review for FGF-8 (423-F8) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using 423-F8:
Filter by Applications
Stem/Immune cell maintenance or differentiation
(1)
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Stem/Immune cell maintenance or differentiation  FGF-8 423-F8
Enlarge
5
reviewed by:
Verified Customer
Stem/Immune cell maintenance or differentiation 09/11/2019
View

Summary

ApplicationStem/Immune cell maintenance or differentiation
LotAFK2714121
CommentsUsed for differentiation of human iPSC to dopaminergic neurons. easy to reconstitute and wasn't toxic to cells.

FAQs for FGF-8 (423-F8) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional FGF-8 Products

Blogs on FGF-8

There are no specific blogs for FGF-8, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
09/11/2019
Application: Stem/Immune cell maintenance or differentiation
Species:

Bioinformatics