Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus

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1 μg/lane of Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus (Catalog # 464-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.05-0.25 μg/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.05-0.25 µg/mL.
Source
E. coli-derived mouse Sonic Hedgehog/Shh protein
Cys25-Gly198 (Cys25Ile-Ile), with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Shh
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
464-SH in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and DTT with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT
  • TPTPS

Background

Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Mouse Shh encodes a 437 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).
  1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
  2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
  3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
  4. Echelard, Y. et al. (1993) Cell 75:1417.
  5. Zeng, X. et al. (2001) Nature 411:716.
  6. Feng, J. et al. (2004) Development 131:4357.
  7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
  8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
  9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
  10. Etheridge, L.A. et al. (2010) Development 137:133.
  11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
  12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
  13. Lewis, P.M. et al. (2001) Cell 105:599.
  14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
  15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
  16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.

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464-SH
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Applications: Bioactivity

Publications for Sonic Hedgehog/Shh (464-SH)(28)

We have publications tested in 4 confirmed species: Human, Mouse, Chicken, Zebrafish.

We have publications tested in 3 applications: Bioassay, In Vivo, tissue culture.


Filter By Application
Bioassay
(26)
In Vivo
(1)
tissue culture
(1)
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Filter By Species
Human
(14)
Mouse
(11)
Chicken
(1)
Zebrafish
(2)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using 464-SH Applications Species
S Grabow, AJ Kueh, F Ke, HK Vanyai, BN Sheikh, MA Dengler, W Chiang, S Eccles, IM Smyth, LK Jones, FJ de Sauvage, M Scott, L Whitehead, AK Voss, A Strasser Subtle Changes in the Levels of BCL-2 Proteins Cause Severe Craniofacial Abnormalities Cell Rep, 2018;24(12):3285-3295.e4. 2018 [PMID: 30232009] (Bioassay, Mouse) Bioassay Mouse
N Tasnim, V Thakur, M Chattopadh, B Joddar The Efficacy of Graphene Foams for Culturing Mesenchymal Stem Cells and Their Differentiation into Dopaminergic Neurons Stem Cells Int, 2018;2018(0):3410168. 2018 [PMID: 29971110] (Bioassay, Mouse) Bioassay Mouse
JL Everson, DM Fink, HM Chung, MR Sun, RJ Lipinski Identification of sonic hedgehog-regulated genes and biological processes in the cranial neural crest mesenchyme by comparative transcriptomics BMC Genomics, 2018;19(1):497. 2018 [PMID: 29945554] (Bioassay, Mouse) Bioassay Mouse
B Wang, JH Joo, R Mount, BJW Teubner, A Krenzer, AL Ward, VP Ichhaporia, EJ Adams, R Khoriaty, ST Peters, SM Pruett-Mil, SS Zakharenko, D Ginsburg, M Kundu The COPII cargo adapter SEC24C is essential for neuronal homeostasis J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29939162] (Bioassay, Human) Bioassay Human
G Collo, L Cavalleri, M Zoli, U Maskos, E Ratti, E Merlo Pich Alpha6-Containing Nicotinic Acetylcholine Receptors Mediate Nicotine-Induced Structural Plasticity in Mouse and Human iPSC-Derived Dopaminergic Neurons Front Pharmacol, 2018;9(0):572. 2018 [PMID: 29910731] (Bioassay, Human) Bioassay Human
YK Wang, WW Zhu, MH Wu, YH Wu, ZX Liu, LM Liang, C Sheng, J Hao, L Wang, W Li, Q Zhou, BY Hu Human Clinical-Grade Parthenogenetic ESC-Derived Dopaminergic Neurons�Recover Locomotive Defects of Nonhuman Primate Models of�Parkinson&#039;s Disease Stem Cell Reports, 2018;0(0):. 2018 [PMID: 29910127] (Bioassay, Human) Bioassay Human
JC Niclis, CW Gantner, CPJ Hunt, JA Kauhausen, JC Durnall, JM Haynes, CW Pouton, CL Parish, LH Thompson A PITX3-EGFP Reporter Line Reveals Connectivity of Dopamine and Non-dopamine Neuronal Subtypes in Grafts Generated from Human Embryonic Stem Cells Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28867345] (Bioassay, Human) Bioassay Human
Y Han, Y Xiong, X Shi, J Wu, Y Zhao, J Jiang Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system PLoS Biol., 2017;15(8):e2002063. 2017 [PMID: 28777795] (Bioassay, Mouse) Bioassay Mouse
Y Xiang, Y Tanaka, B Patterson, YJ Kang, G Govindaiah, N Roselaar, B Cakir, KY Kim, AP Lombroso, SM Hwang, M Zhong, EG Stanley, AG Elefanty, JR Naegele, SH Lee, SM Weissman, IH Park Fusion of Regionally Specified hPSC-Derived Organoids Models Human Brain Development and Interneuron Migration Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28757360] (Bioassay, Human) Bioassay Human
S Baronchell, A La Spada, A Ntai, A Barbieri, P Conforti, GS Jotti, S Redaelli, A Bentivegna, P De Blasio, I Biunno Epigenetic and transcriptional modulation of WDR5, a chromatin remodeling protein, in Huntington&#039;s disease human induced pluripotent stem cell (hiPSC) model Mol. Cell. Neurosci., 2017;82(0):46-57. 2017 [PMID: 28476540] (Bioassay, Human) Bioassay Human
Show All 28 Publications.

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Bioinformatics

Gene Symbol Shh
Uniprot