beta-Galactosidase-1/GLB1 Antibody (OTI1C9)

Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLB1 .

Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu, Ca, Pm
• Applications: WB, IHC
• Clone: OTI1C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

beta-Galactosidase-1/GLB1 Antibody (OTI1C9) Summary

• Immunogen: Full length human recombinant protein of human GLB1(NP_001073279) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GLB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500
• Theoretical MW: 72.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1% BSA, 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for beta-Galactosidase-1/GLB1 Antibody (OTI1C9)

• Acid beta-galactosidase
• beta-galactosidase
• betaGalactosidase1
• beta-Galactosidase-1
• EBP
• EC 3.2.1.23
• elastin receptor 1 (67kD)
• Elastin receptor 1
• elastin receptor 1, 67kDa
• ELNR1
• galactosidase, beta 1
• GLB1
• Lactase
• MPS4B

Background

The GLB1 gene encodes beta-galactosidase-1 (EC 3.2.1.23), a lysosomal hydrolase that cleaves the terminal beta-galactose from ganglioside substrates and other glycoconjugates (Yoshida et al., 1991 [PubMed 1907800]). Beta-galactosidase also occurs in a complex with neuraminidase (NEU1; MIM 608272) and protective protein/cathepsin A (PPCA; MIM 256540), which is a component of certain cell surface receptors (Hinek, 1996 [PubMed 8922281]). See also galactosylceramidase (GALC; MIM 606890) (EC 3.2.1.46), a genetically distinct beta-galactosidase that is involved in the catabolism of other lipid compounds.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-Galactosidase-1/GLB1 Antibody (NBP2-45731)(6)

Publications using NBP2-45731

• Limberger T, Schlederer M, TrachtovA K et al. KMT2C methyltransferase domain regulated INK4A expression suppresses prostate cancer metastasis Molecular Cancer 2022-12-01 [PMID: 35354467] (IHC-P, Mouse)
• Lee TXY, Wu J, Jean WH et al. Reduced stem cell aging in exercised human skeletal muscle is enhanced by ginsenoside Rg1 Aging 2021-06-28 [PMID: 34181580] (IHC-P)
• Wu J, Cheng IS, Saovieng S et al. Aerobic exercise induces tumor suppressor p16INK4a expression of endothelial progenitor cells in human skeletal muscle Aging (Albany NY) 2020-10-26 [PMID: 33104519] (IF/IHC, Human)
• Luu AR, Wong H, Agrawal V et al. Lysosome-targeted beta-galactosidase negatively regulates neuraminidase 1 (NEU1) and promotes NEU1 deficiency in GM1 gangliosidosis J. Biol. Chem. 2020-07-28 [PMID: 32727849] (WB, Non-species specific)
• Wu J, Saovieng S et al. Ginsenoside Rg1 supplementation clears senescence-associated beta-galactosidase in exercising human skeletal muscle. J Ginseng Res 2019-01-10 [PMID: 31695564] (ICC/IF, Human)
• Chen JC, Luu AR, Wise N et al. Intracerebroventricular enzyme replacement therapy with Beta-Galactosidase reverses brain pathologies due to GM1 gangliosidosis in mice J. Biol. Chem. 2019-09-03 [PMID: 31481471] (WB)

Bioinformatics

• Gene Symbol: GLB1