Wnt-7a Antibody

Western Blot: Wnt-7a Antibody [NBP2-20913] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT7A antibody diluted at 1:5000.

Immunohistochemistry-Paraffin: Wnt-7a Antibody [NBP2-20913] - Mouse stomach. WNT7A antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Wnt-7a Antibody [NBP2-20913] - Mouse pancreas. WNT7A antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Bv, Ch, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Wnt-7a Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human WNT7A. The exact sequence is proprietary.
• Localization: Secreted; extracellular space; extracellular matrix
• Predicted Species: Rat (100%), Chicken (96%), Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WNT7A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for Wnt-7a Antibody

• protein Wnt-7a
• proto-oncogene Wnt7a protein
• wingless-type MMTV integration site family, member 7A
• Wnt7a
• Wnt-7a

Background

This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndromes. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Wnt-7a Antibody (NBP2-20913)(1)

Publication using NBP2-20913

• Wellenstein MD Distinct p53 hotspot mutations differentially regulate immunotherapy response in breast cancer through activation of autophagy Thesis 2022-01-01 (IF/IHC)

Bioinformatics

• Gene Symbol: WNT7A