GLI-3 Antibody

Images

 
Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) followed by HRP-conjugated ...read more
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. ...read more
GLI‑3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) at 10 µg/mL for 3 hours at room ...read more
Conditional deletion of GATA6 does not significantly alter Gli3 processing in the hindlimb bud.(A) Western blot analysis of Gli3FL and Gli3R steady state levels in anterior (lanes 1 and 3) or posterior (lanes 2 and 4) ...read more
SufuT396I does not stabilize Gli3FL protein and reduces the processing of Gli3FL.(A) Western blotting of lysates prepared from SufuR146X/R146X at E9.5 and SufuT396I/T396I, SufuT396I/+, and wild-type embryos at E10.5 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

GLI-3 Antibody Summary

Immunogen
E. coli-derived recombinant human GLI-3
Met1-Glu479
Accession # P10071
Specificity
Detects human and mouse GLI-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GLI3
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF3690 in the following applications:

Publications
Read Publications using
AF3690 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLI-3 Antibody

  • ACLS
  • ACLSPAPAPHS
  • ADD
  • Bph
  • GCPS
  • GLI family zinc finger 3
  • GLI3 form of 190 kDa
  • GLI3 full length protein
  • GLI3
  • GLI-3
  • GLI3-190
  • GLI3FL
  • GLI-Kruppel family member GLI3
  • glioma-associated oncogene family zinc finger 3
  • Greig cephalopolysyndactyly syndrome
  • oncogene GLI3
  • PAP-A
  • PAPA1
  • PAPB
  • PAPBDNA-binding protein
  • Pdn
  • PHS
  • PPDIV
  • Xt
  • zinc finger protein GLI3

Background

Human GLI-3 is a 190 kDa transcription factor of the C2H2-type zinc-finger protein family. GLI-3 appears to play a role in limb and brain development, probably through sonic hedgehog signaling. GLI-3 is regulated by PKA-mediated phosphorylation. Unphosphorylated full-length GLI-3 is a transcriptional activator, while phosphorylated GLI-3 is cleaved after amino acid 700 to generate an N-terminal 83 kDa transcriptional repressor. Over the region used as immunogen, human GLI-3 is 95% identical to the corresponding mouse and canine protein sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GLI-3 Antibody (AF3690)(45)

We have publications tested in 4 confirmed species: Human, Mouse, C. elegans, Transgenic Mouse.

We have publications tested in 8 applications: Bioassay, CUT&Tag, ChIP, ICC, IHC, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
Bioassay
(1)
CUT&Tag
(1)
ChIP
(1)
ICC
(5)
IHC
(7)
IHC-P
(3)
Immunoprecipitation
(3)
Western Blot
(32)
All Applications
Filter By Species
Human
(10)
Mouse
(34)
C. elegans
(2)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using AF3690 Applications Species
JS Fleck, SMJ Jansen, D Wollny, F Zenk, M Seimiya, A Jain, R Okamoto, M Santel, Z He, JG Camp, B Treutlein Inferring and perturbing cell fate regulomes in human brain organoids Nature, 2022-10-05;0(0):. 2022-10-05 [PMID: 36198796] (Bioassay, CUT&Tag, IHC, Human) Bioassay, CUT&Tag, IHC Human
M Kurtovi?, N Piteša, N Bartoni?ek, P Ozreti?, V Musani, J ?onkaš, T Petri?, C King, M Sabol RNA-seq and ChIP-seq Identification of Unique and Overlapping Targets of GLI Transcription Factors in Melanoma Cell Lines Cancers, 2022-09-19;14(18):. 2022-09-19 [PMID: 36139698] (ChIP, Human) ChIP Human
S Schmidt, MD Luecken, D Trümbach, S Hembach, KM Niedermeie, N Wenck, K Pflügler, C Stautner, A Böttcher, H Lickert, C Ramirez-Su, R Ahmad, MJ Ziller, JC Fitzgerald, V Ruf, WDJ van de Ber, AJ Jonker, T Gasser, B Winner, J Winkler, DM Vogt Weise, F Giesert, FJ Theis, W Wurst Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease Nature Communications, 2022-08-16;13(1):4819. 2022-08-16 [PMID: 35974013] (IHC, Human) IHC Human
J Sun, J Yoon, M Lee, HK Lee, YS Hwang, IO Daar Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development Cell Reports, 2022-02-01;38(5):110312. 2022-02-01 [PMID: 35108539] (Immunoprecipitation, Western Blot, Human) Immunoprecipitation, Western Blot Human
D Sharma, AJ Mirando, A Leinroth, JT Long, CM Karner, MJ Hilton HES1 is a novel downstream modifier of the SHH-GLI3 Axis in the development of preaxial polydactyly PloS Genetics, 2021-12-20;17(12):e1009982. 2021-12-20 [PMID: 34928956] (Western Blot, Mouse, Transgenic Mouse) Western Blot Mouse, Transgenic Mouse
A Koeniger, A Brichkina, I Nee, L Dempwolff, A Hupfer, I Galperin, F Finkernage, A Nist, T Stiewe, T Adhikary, W Diederich, M Lauth Activation of Cilia-Independent Hedgehog/GLI1 Signaling as a Novel Concept for Neuroblastoma Therapy Cancers, 2021-04-15;13(8):. 2021-04-15 [PMID: 33921042] (Western Blot, Mouse) Western Blot Mouse
KH Elliott, X Chen, J Salomone, P Chaturvedi, PA Schultz, SK Balchand, JD Servetas, A Zuniga, R Zeller, B Gebelein, MT Weirauch, KA Peterson, SA Brugmann Gli3 utilizes Hand2 to synergistically regulate tissue-specific transcriptional networks Elife, 2020-10-02;9(0):. 2020-10-02 [PMID: 33006313] (Western Blot, Mouse) Western Blot Mouse
JM Dias, Z Alekseenko, A Jeggari, M Boareto, J Vollmer, M Kozhevniko, H Wang, MP Matise, A Alexeyenko, D Iber, J Ericson A Shh/Gli-driven three-node timer motif controls temporal identity and fate of neural stem cells Science Advances, 2020-09-16;6(38):. 2020-09-16 [PMID: 32938678] (ICC, Immunoprecipitation, Western Blot, Mouse) ICC, Immunoprecipitation, Western Blot Mouse
RU Chottekala, S Kalik, J Gresack, A Ayala, M Gao, W Wang, S Meller, A Aly, A Schaefer, P Greengard AP-1 controls the p11-dependent antidepressant response Mol. Psychiatry, 2020-05-21;0(0):. 2020-05-21 [PMID: 32439846] (IHC, C. elegans) IHC C. elegans
H Yan, C Chen, H Chen, H Hong, Y Huang, K Ling, J Hu, Q Wei TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating Nat Commun, 2020-05-04;11(1):2196. 2020-05-04 [PMID: 32366837] (IHC, C. elegans) IHC C. elegans
Show All 45 Publications.

Reviews for GLI-3 Antibody (AF3690) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using AF3690:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot GLI-3 AF3690
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 11/13/2015
View

Summary

ApplicationWestern Blot
Sample TestedNIH-3T3 cells
SpeciesMouse
LotYLD0109011
  5
reviewed by:
Verified Customer
WB Mouse 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22173325
SpeciesMouse
  4
reviewed by:
Verified Customer
WB Mouse 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22547067
SpeciesMouse

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FAQs for GLI-3 Antibody (AF3690) (0)

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Recent Reviews

4.7
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Verified Customer
11/13/2015
Application: WB
Species: Mouse

 
Verified Customer
01/08/2015
Application: WB
Species: Mouse

 
Verified Customer
01/08/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol GLI3
Uniprot