GLI-3 Antibody

Images

 
Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) followed by HRP-conjugated ...read more
GLI‑3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) at 10 µg/mL for 3 hours at room ...read more
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

GLI-3 Antibody Summary

Immunogen
E. coli-derived recombinant human GLI-3
Met1-Glu479
Accession # P10071
Specificity
Detects human and mouse GLI-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GLI3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF3690 in the following applications:

Publications
Read Publications using
AF3690 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLI-3 Antibody

  • ACLS
  • ACLSPAPAPHS
  • ADD
  • Bph
  • GCPS
  • GLI family zinc finger 3
  • GLI3 form of 190 kDa
  • GLI3 full length protein
  • GLI3
  • GLI-3
  • GLI3-190
  • GLI3FL
  • GLI-Kruppel family member GLI3
  • glioma-associated oncogene family zinc finger 3
  • Greig cephalopolysyndactyly syndrome
  • oncogene GLI3
  • PAP-A
  • PAPA1
  • PAPB
  • PAPBDNA-binding protein
  • Pdn
  • PHS
  • PPDIV
  • Xt
  • zinc finger protein GLI3

Background

Human GLI-3 is a 190 kDa transcription factor of the C2H2-type zinc-finger protein family. GLI-3 appears to play a role in limb and brain development, probably through sonic hedgehog signaling. GLI-3 is regulated by PKA-mediated phosphorylation. Unphosphorylated full-length GLI-3 is a transcriptional activator, while phosphorylated GLI-3 is cleaved after amino acid 700 to generate an N-terminal 83 kDa transcriptional repressor. Over the region used as immunogen, human GLI-3 is 95% identical to the corresponding mouse and canine protein sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GLI-3 Antibody (AF3690)(27)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ICC, IHC - Paraffin embedded, IHC Paraffin-embedded, IHC-P, IP, WB.


Filter By Application
ICC
(1)
IHC - Paraffin embedded
(1)
IHC Paraffin-embedded
(1)
IHC-P
(1)
IP
(1)
WB
(20)
All Applications
Filter By Species
Human
(3)
Mouse
(22)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using AF3690 Applications Species
SR Hong, CL Wang, YS Huang, YC Chang, YC Chang, GV Pusapati, CY Lin, N Hsu, HC Cheng, YC Chiang, WE Huang, NC Shaner, R Rohatgi, T Inoue, YC Lin Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling Nat Commun, 2018;9(1):1732. 2018 [PMID: 29712905] (ICC,, Mouse) ICC Mouse
A Struchtrup, A Wiegering, B Stork, U Rüther, C Gerhardt The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts Autophagy, 2018;0(0):1-47. 2018 [PMID: 29372668] (WB,, Mouse) WB Mouse
LK Veistinen, T Mustonen, MR Hasan, M Takatalo, Y Kobayashi, DA Kesper, A Vortkamp, DP Rice Regulation of Calvarial Osteogenesis by Concomitant De-repression of GLI3 and Activation of IHH Targets Front Physiol, 2017;8(0):1036. 2017 [PMID: 29311969] (WB,, Mouse) WB Mouse
VJ Guen, TE Chavarria, C Kröger, X Ye, RA Weinberg, JA Lees EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 29158396] (IHC-P,, Mouse) IHC-P Mouse
D Hasegawa, H Ochiai-Shi, S Onodera, T Nakamura, A Saito, T Onda, K Watanabe, K Nishimura, M Ohtaka, M Nakanishi, K Kosaki, A Yamaguchi, T Shibahara, T Azuma Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction PLoS ONE, 2017;12(10):e0186879. 2017 [PMID: 29088246]
J Li, Y Cui, J Xu, Q Wang, X Yang, Y Li, X Zhang, M Qiu, Z Zhang, Z Zhang Suppressor of Fused Restraint of Hedgehog Activity Level is Critical for Osteogenic Proliferation and Differentiation during Calvarial Bone Development J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28794157] (WB,, Mouse) WB Mouse
Y Han, Y Xiong, X Shi, J Wu, Y Zhao, J Jiang Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system PLoS Biol., 2017;15(8):e2002063. 2017 [PMID: 28777795] (WB,, Mouse) WB Mouse
AM Arensdorf, ME Dillard, JM Menke, MW Frank, CO Rock, SK Ogden Sonic Hedgehog Activates Phospholipase A2 to Enhance Smoothened Ciliary Translocation Cell Rep, 2017;19(10):2074-2087. 2017 [PMID: 28591579] (WB,, Mouse) WB Mouse
H Cai, A Liu Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord Dev. Biol., 2017;0(0):. 2017 [PMID: 28412462] (WB,, Mouse) WB Mouse
Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9 Sci Rep, 2016;6(0):39608. 2016 [PMID: 28000783] (WB,, Mouse) WB Mouse
Show All 27 Publications.

Reviews for GLI-3 Antibody (AF3690) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using AF3690:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot GLI-3 AF3690
Enlarge
reviewed by:
Anonymous
WB Mouse 11/13/2015
View

Summary

ApplicationWestern Blot
Sample TestedNIH-3T3 cells
SpeciesMouse
LotYLD0109011

Blocking

Blocking Details5% milk in 1xTBS-Tween (0.1%) for 30 min at room temperature

Primary Anitbody

Dilution Ratio1:200 dilution in blocking buffer; incubation at 4 deg on a rotator for 16 hr

Secondary Antibody

Secondary DescriptionPeroxidase AffiniPure Donkey Anti-Goat IgG (H+L)
Secondary Manufacturer Cat#705-035-003
Secondary Concentration1:10,000 dilution

Details

Detection NotesECL pico/femto; exposure time was 3 min; Amersham Hyperfilm ECL film was used

Comments

CommentsNIH-3T3 cells were seeded on a 6 well plate and at 100% confluency, cells were serum starved for 24 hr. Hedgehog activating reagents (Shh conditioned medium or SAG) were also include at the same time. Hedgehog pathway activation results in loss of Gli3 repressor formation, a feature that this antibody nicely shows in this blot. This antibody detects both the full length and repressor forms of Gli3.
 
reviewed by:
Anonymous
WB Mouse 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22173325
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22173325

Details

Detection NotesSee PMID 22173325

Comments

CommentsPublished: See PMID 22173325Independent review from antibodyresource.com
 
reviewed by:
Anonymous
WB Mouse 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22547067
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22547067

Details

Detection NotesSee PMID 22547067

Comments

CommentsPublished: See PMID 22547067Independent review from antibodyresource.com

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Anonymous
11/13/2015
Application: WB
Species: Mouse

 
Anonymous
01/08/2015
Application: WB
Species: Mouse

 
Anonymous
01/08/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol GLI3
Entrez
Uniprot