HOXD13 Antibody

Immunocytochemistry/Immunofluorescence: HOXD13 Antibody [NBP1-52149] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry-Paraffin: HOXD13 Antibody [NBP1-52149] - Staining of Human Prostate. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunocytochemistry/Immunofluorescence: HOXD13 Antibody [NBP1-52149] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL). EB09833 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue).

Microarray: HOXD13 Antibody [NBP1-52149] - Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma. Microarray image sumbitted by a verified customer review.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: ELISA, ICC/IF, IHC, MA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HOXD13 Antibody Summary

• Immunogen: Peptide with sequence C-KSSFPGDVALNQPD corresponding to internal region according to NP_000514.2.
• Epitope: C-KSSFPGDVALNQPD
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HOXD13
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 1 - 2 ug/mL
  • Immunohistochemistry-Paraffin 1 - 2 ug/mL
  • Microarray
  • Peptide ELISA Detection limit 1:8000
• Application Notes: This HOXD13 Antibody is validated for microarray from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HOXD13 Antibody

• BDSD
• homeo box 4I
• homeo box D13
• homeobox D13
• Homeobox protein Hox-4I
• homeobox protein Hox-D13
• HOX4IBDE
• SPD

Background

This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. Mutations in this particular gene cause synpolydactyly. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HOXD13 Antibody (NBP1-52149)(1)

Publication using NBP1-52149

• Cantile M, Franco R, Tschan A et al. HOX D13 expression across 79 tumor tissue types. Int J Cancer 2009-10-01 [PMID: 19488988]

Review for HOXD13 Antibody (NBP1-52149) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Carly Garrison MiAr Human 12/07/2020

Summary

• Application: Microarray
• Sample Tested: EDTA Plasma
• Species: Human
• Lot: P1 N230610

Bioinformatics

• Gene Symbol: HOXD13
• Entrez:
  • 3239(Human)
  • 15433(Mouse)
  • 288154(Rat)
• Uniprot:
  • NP_000514.2(Human)