TRF-1 Antibody (57-6)

Images

 
Biological Strategies: Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) mimic in rat vascular smooth muscle cells (A7r5) suppressed angiogenesis and proliferation but promoted ...read more
Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - An intracellular stain was performed on HeLa with NB110-68281 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with ...read more
Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1 in HeLa whole cell extracts.
Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1. 50 ug of total lysate each lane.
Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using TRF1 antibody (dark blue). Isotype control shown in orange. An antibody ...read more
Simple Western: TRF-1 Antibody (57-6) [NB110-68281] - Image shows a specific band for TRF1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF (-)
Clone
57-6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

TRF-1 Antibody (57-6) Summary

Immunogen
Recombinant human TRF1 [UniProt# P54274]
Localization
Nucleus
Marker
Telomere Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TERF1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Simple Western 1:200
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1 ug per million cells
Application Notes
This TRF1 antibody is useful for ELISA and Western blot, where a band can be seen at approx. 60 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Nuclear Cell Lysate
HeLa Whole Cell Lysate
TRF-1 Overexpression Lysate
Publications
Read Publications using
NB110-68281 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TRF-1 Antibody (57-6)

  • NIMA-interacting protein 2
  • PIN-2
  • PIN2hTRF1-AS
  • Telomeric protein Pin2/TRF1
  • telomeric repeat binding factor (NIMA-interacting) 1
  • telomeric repeat binding factor 1
  • telomeric repeat binding protein 1
  • telomeric repeat-binding factor 1
  • TERF1
  • TRBF1
  • TRF1
  • TRF-1
  • TRF1FLJ41416
  • TRFt-TRF1
  • TTAGGG repeat-binding factor 1

Background

TRF1 (telomeric repeat-binding factor 1) is a protein that binds telomeric double-stranded TTAGGG repeat and is implicated in telomere replication, telomere protection, telomere length maintenance, resolution of sister telomeres and mitotic spindle regulation. TRF1 interacts directly with TIN2 in shelterin complex (TRF1, TRF2, TIN2, POT1, TPP1 and hRap1; a complex that coats telomeres for their protection and regulation of telomere length) and it then binds to TRF2/TPP1. While POT1 binds to TPP1, TRF2 interacts and forms a complex with hRap1. Upon ionizing DNA damage, TRF1 gets phosphorylated (ser-219) in ATM-dependent manner. ADP-ribosylation by TNKS1 or TNKS2 diminishes TRF1's telomeric DNA binding ability, whereas, RLIM/RNF12 as well as SCF (SKP1-CUL1-F-box protein) can ubiquinate TRF1 for its degradation. TRF1 is ubiquitously expressed and during the cell cycle, its levels are low in G1/S phases which may increase during G2/M phases. Overexpression of TRF1 promotes telomere shortening whereas loss of TRF1 accumulate telomere fusions and induce telomerase-dependent telomere lengthening, implying that TRF1 negatively regulates telomerase-dependent telomere extension, perhaps by restricting the access of telomerase to the ends of telomeres. TRF1 knockout leads to embroynic lethality and deletion of TRF1 promotes the formation of fragile telomeres.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRF-1 Antibody (NB110-68281)(3)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
All Applications
Filter By Species
Human
(1)
Rat
(1)
All Species
Showing Publications 1 - 3 of 3.
Publications using NB110-68281 Applications Species
Sharma S, Mukherjee AK, Roy SS et al. Human Telomerase Expression is under Direct Transcriptional Control of the Telomere-binding-factor TRF2 bioRxiv Jan 1 2020
Chen YL, Sheu JJ, Sun CK et al. MicroRNA-214 modulates the senescence of vascular smooth muscle cells in carotid artery stenosis Mol. Med. May 14 2020 [PMID: 32410577] (WB, Rat) WB Rat
Jullien L, Mestre M, Roux P, Gire V. Eroded human telomeres are more prone to remain uncapped and to trigger a G2 checkpoint response Nucleic Acids Res 2012 Nov 27 [PMID: 23193277] (WB, Human) WB Human

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Product General Protocols

View specific protocols for TRF-1 Antibody (NB110-68281): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TRF-1 Antibody (NB110-68281) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-68281AF350
Alexa Fluor 405 NB110-68281AF405
Alexa Fluor 488 NB110-68281AF488
Alexa Fluor 532 NB110-68281AF532
Alexa Fluor 594 NB110-68281AF594
Alexa Fluor 647 NB110-68281AF647
Alexa Fluor 700 NB110-68281AF700
Alexa Fluor 750 NB110-68281AF750
Allophycocyanin NB110-68281APC
Biotin NB110-68281B
DyLight 350 NB110-68281UV
DyLight 405 NB110-68281V
DyLight 488 NB110-68281G
DyLight 550 NB110-68281R
DyLight 594 NB110-68281DL594
DyLight 650 NB110-68281C
DyLight 680 NB110-68281FR
DyLight 755 NB110-68281IR
FITC NB110-68281F
HRP NB110-68281H
PE NB110-68281PE
PerCP NB110-68281PCP

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Bioinformatics

Gene Symbol TERF1