TRF-1 Antibody (57-6)


Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1. 50 ug of total lysate each lane.
Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - An intracellular stain was performed on HeLa with NB110-68281 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with more
Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1 in HeLa whole cell extracts.
Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using TRF1 antibody (dark blue). Isotype control shown in orange. An antibody more
Simple Western: TRF-1 Antibody (57-6) [NB110-68281] - Image shows a specific band for TRF1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF (-)
1.5 mg/ml

TRF-1 Antibody (57-6) Summary

Recombinant human TRF1 [UniProt# P54274]
Telomere Marker
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.5 ug/mL
  • Simple Western 1:200
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1 ug per million cells
Application Notes
This TRF1 antibody is useful for ELISA and Western blot, where a band can be seen at approx. 60 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
HeLa Lysate (NB800-PC9)
HeLa Lysate (NB800-PC1)
TRF-1 Lysate (NBL1-16814)
Read Publication using
NB110-68281 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine, 0.15M NaCl
0.05% Sodium Azide
1.5 mg/ml
Protein G purified

Alternate Names for TRF-1 Antibody (57-6)

  • NIMA-interacting protein 2
  • PIN-2
  • PIN2hTRF1-AS
  • Telomeric protein Pin2/TRF1
  • telomeric repeat binding factor (NIMA-interacting) 1
  • telomeric repeat binding factor 1
  • telomeric repeat binding protein 1
  • telomeric repeat-binding factor 1
  • TERF1
  • TRBF1
  • TRF1
  • TRF-1
  • TRF1FLJ41416
  • TRFt-TRF1
  • TTAGGG repeat-binding factor 1


TRF1 (telomeric repeat-binding factor 1) is a protein that binds telomeric double-stranded TTAGGG repeat and is implicated in telomere replication, telomere protection, telomere length maintenance, resolution of sister telomeres and mitotic spindle regulation. TRF1 interacts directly with TIN2 in shelterin complex (TRF1, TRF2, TIN2, POT1, TPP1 and hRap1; a complex that coats telomeres for their protection and regulation of telomere length) and it then binds to TRF2/TPP1. While POT1 binds to TPP1, TRF2 interacts and forms a complex with hRap1. Upon ionizing DNA damage, TRF1 gets phosphorylated (ser-219) in ATM-dependent manner. ADP-ribosylation by TNKS1 or TNKS2 diminishes TRF1's telomeric DNA binding ability, whereas, RLIM/RNF12 as well as SCF (SKP1-CUL1-F-box protein) can ubiquinate TRF1 for its degradation. TRF1 is ubiquitously expressed and during the cell cycle, its levels are low in G1/S phases which may increase during G2/M phases. Overexpression of TRF1 promotes telomere shortening whereas loss of TRF1 accumulate telomere fusions and induce telomerase-dependent telomere lengthening, implying that TRF1 negatively regulates telomerase-dependent telomere extension, perhaps by restricting the access of telomerase to the ends of telomeres. TRF1 knockout leads to embroynic lethality and deletion of TRF1 promotes the formation of fragile telomeres.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRF-1 Antibody (NB110-68281)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-68281AF405
Alexa Fluor 488 Labeled NB110-68281AF488
Alexa Fluor 647 Labeled NB110-68281AF647
Alexa Fluor 700 Labeled NB110-68281AF700
Biotin Labeled NB110-68281B
DyLight 405 Labeled NB110-68281V
DyLight 488 Labeled NB110-68281G
DyLight 550 Labeled NB110-68281R
DyLight 650 Labeled NB110-68281C
FITC Labeled NB110-68281F
HRP Labeled NB110-68281H

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Gene Symbol TERF1