TRF-1 Antibody (57-6)

Images

 
Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) mimic in rat vascular smooth muscle cells (A7r5) suppressed angiogenesis and proliferation but promoted senescence. Representative western ...read more
Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - An intracellular stain was performed on HeLa with NB110-68281 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with ...read more
Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1 in HeLa whole cell extracts.
Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1. 50 ug of total lysate each lane.
Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using TRF1 antibody (dark blue). Isotype control shown in orange. An antibody ...read more
Simple Western: TRF-1 Antibody (57-6) [NB110-68281] - Image shows a specific band for TRF1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Striking muscle pathology of gastrocnemius in Tg mice from Fo. 1579. a Transverse and b longitudinal sections from phenotypic Tg mouse stained with H&E compared to c transverse and d longitudinal of NT mouse. e Matrin 3 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF
Clone
57-6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TRF-1 Antibody (57-6) Summary

Immunogen
Recombinant human TRF1 [UniProt# P54274]
Localization
Nucleus
Marker
Telomere Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TERF1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence 2 ug/ml
  • Simple Western 1:200
  • Western Blot 0.5 ug/ml
Application Notes
This TRF1 antibody is useful for ELISA and Western blot, where a band can be seen at approx. 60 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Nuclear Cell Lysate
HeLa Whole Cell Lysate
TRF-1 Overexpression Lysate
Publications
Read Publications using
NB110-68281 in the following applications:

  • B/N
    1 publication
  • 1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TRF-1 Antibody (57-6)

  • NIMA-interacting protein 2
  • PIN-2
  • PIN2hTRF1-AS
  • Telomeric protein Pin2/TRF1
  • telomeric repeat binding factor (NIMA-interacting) 1
  • telomeric repeat binding factor 1
  • telomeric repeat binding protein 1
  • telomeric repeat-binding factor 1
  • TERF1
  • TRBF1
  • TRF1
  • TRF-1
  • TRF1FLJ41416
  • TRFt-TRF1
  • TTAGGG repeat-binding factor 1

Background

TRF1 (telomeric repeat-binding factor 1) is a protein that binds telomeric double-stranded TTAGGG repeat and is implicated in telomere replication, telomere protection, telomere length maintenance, resolution of sister telomeres and mitotic spindle regulation. TRF1 interacts directly with TIN2 in shelterin complex (TRF1, TRF2, TIN2, POT1, TPP1 and hRap1; a complex that coats telomeres for their protection and regulation of telomere length) and it then binds to TRF2/TPP1. While POT1 binds to TPP1, TRF2 interacts and forms a complex with hRap1. Upon ionizing DNA damage, TRF1 gets phosphorylated (ser-219) in ATM-dependent manner. ADP-ribosylation by TNKS1 or TNKS2 diminishes TRF1's telomeric DNA binding ability, whereas, RLIM/RNF12 as well as SCF (SKP1-CUL1-F-box protein) can ubiquinate TRF1 for its degradation. TRF1 is ubiquitously expressed and during the cell cycle, its levels are low in G1/S phases which may increase during G2/M phases. Overexpression of TRF1 promotes telomere shortening whereas loss of TRF1 accumulate telomere fusions and induce telomerase-dependent telomere lengthening, implying that TRF1 negatively regulates telomerase-dependent telomere extension, perhaps by restricting the access of telomerase to the ends of telomeres. TRF1 knockout leads to embroynic lethality and deletion of TRF1 promotes the formation of fragile telomeres.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRF-1 Antibody (NB110-68281)(6)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TERF1