| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-68281SS | |||
| Azide and BSA Free | NBP2-80993 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1701-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57285-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21712-50ul | |||
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) mimic in rat vascular smooth muscle cells (A7r5) suppressed angiogenesis and proliferation but promoted senescence. Representative western blots depicting TERF1, TERF2, and quaking expression in mimic NC or miR-214 mimic transfected cells. Image collected and cropped by CiteAb from the following publication (//molmed.biomedcentral.com/articles/10.1186/s10020-020-00167-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0012.jpg "Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) mimic in rat vascular smooth muscle cells (A7r5) suppressed angiogenesis and proliferation but promoted senescence. Representative western blots depicting TERF1, TERF2, and quaking expression in mimic NC or miR-214 mimic transfected cells. Image collected and cropped by CiteAb from the following publication (//molmed.biomedcentral.com/articles/10.1186/s10020-020-00167-1), licensed under a CC-BY license.")
![Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - An intracellular stain was performed on HeLa with NB110-68281 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Flow-Cytometry-NB110-68281-img0011.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0003.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1. 50 ug of total lysate each lane.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0005.jpg)
![Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using TRF1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Flow-Cytometry-NB110-68281-img0009.jpg)
![Simple Western: TRF-1 Antibody (57-6) [NB110-68281] - Image shows a specific band for TRF1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Simple-Western-NB110-68281-img0010.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) antagomiR in rat vascular smooth muscle cells (A7r5) induced angiogenesis & proliferation but suppressed senescence. a Schematic of the protocol for transfection of A7r5 VSMCs with a miRNA antagomiR control (antagomiR NC) or a miR-214 antagomiR & processed at the indicated times. b The effects of miR-214 antagomiR on VSMC cell proliferation were tested by CCK-8 assay. c Angiogenetic mRNA expression levels of NOS3, VEGFA, CXCL12 & CXCR4 compared in cells transfected with antagomiR NC or miR-214 antagomiRs (n = 3). d Senescence-associated mRNA expression of TERT, TERF1 & TERF2 compared in cells transfected with antagomiR NC or miR-214 antagomiRs (n = 3). e, G Representative western blots depicting TERF1, TERF2, p16INK4, p21CIP1, pRB, & quaking expression in antagomiR NC or miR-214 antagomiRs transfected cells. f, h Normalized expression of TERF1, TERF2, p16INK4, p21CIP1, pRB, & quaking (n = 3). i Senescence-associated beta -galactosidase staining demonstrating senescence in SCR or miR-214 transfected cells. j Bar graphs show quantification of relative of SA-beta -gal positive cells (n = 3). Data are presented as the means ± SEM. * P < 0.05; ** P < 0.01; ns, non-significance (Two-tailed Student’s t-test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32410577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-68281_mouse-monoclonal-trf-1-antibody-57-6-310202416212346.jpg)
![Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-Western-Blot-NB100-1701-img0007.jpg "Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.")
![Immunocytochemistry/Immunofluorescence: TRF-1 Antibody (TRF-78) - BSA Free [NB100-1701] - HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/TRF-1-Antibody-TRF-78-BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-1701-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: TRF-1 Antibody (4E4) [NBP1-41217] - U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRF1 protein stained by TRF1 antibody [4E4] TRF1 Antibody (4E4) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/TRF-1-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-41217-img0008.jpg "Immunocytochemistry/Immunofluorescence: TRF-1 Antibody (4E4) [NBP1-41217] - U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRF1 protein stained by TRF1 antibody [4E4] TRF1 Antibody (4E4) diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunocytochemistry/Immunofluorescence: TRF-1 Antibody (4E4) [NBP1-41217]](http://images.novusbio.com/fullsize/TRF-1-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-41217-img0006.jpg)
![TRF1 antibody [4E4] detects TRF1 protein at telomere by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TRF1 protein stained by TRF1 antibody [4E4] (NBP1-41217) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-41217_mouse-monoclonal-trf-1-antibody-4e4-immunocytochemistry-immunofluorescence-261120251838405.jpg)
![Immunocytochemistry/Immunofluorescence: TRF-1 Antibody [NBP2-57285] - Staining of human cell line U-2 OS shows localization to nuclear bodies & nucleoli fibrillar center. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TRF-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57285-img0002.jpg "Immunocytochemistry/Immunofluorescence: TRF-1 Antibody [NBP2-57285] - Staining of human cell line U-2 OS shows localization to nuclear bodies & nucleoli fibrillar center. Antibody staining is shown in green.")
![Western Blot: TRF-1 Antibody (SR1896) [NBP3-21712] - Western blot analysis using NBP3-21712 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21712_rabbit-trf-1-mab-sr1896-5720231754878.jpg "Western Blot: TRF-1 Antibody (SR1896) [NBP3-21712] - Western blot analysis using NBP3-21712 in HeLa cell lysate.")
![Western Blot: TRF-1 Antibody [NB100-891] - Analysis of TRF1 in HeLa lysate (RIPA buffer, 30ug total protein per lane) using this antibody at 2 ug/ml. Primary incubated for 12 hour. Detected using chemiluminescence.](http://images.novusbio.com/fullsize/TRF-1-Antibody-Western-Blot-NB100-891-img0004.jpg "Western Blot: TRF-1 Antibody [NB100-891] - Analysis of TRF1 in HeLa lysate (RIPA buffer, 30ug total protein per lane) using this antibody at 2 ug/ml. Primary incubated for 12 hour. Detected using chemiluminescence.")
![Western Blot: TRF-1 Antibody (JE59-70) [NBP3-33048] - Western blot analysis of TRF-1 on different lysates with Rabbit anti-TRF-1 antibody (NBP3-33048) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: U-2 OS cell lysate Lane 4: MCF7 cell lysate Lane 5: RAW264.7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33048) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33048_rabbit-trf-1-mab-je59-70-28820241249340.jpg "Western Blot: TRF-1 Antibody (JE59-70) [NBP3-33048] - Western blot analysis of TRF-1 on different lysates with Rabbit anti-TRF-1 antibody (NBP3-33048) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: U-2 OS cell lysate Lane 4: MCF7 cell lysate Lane 5: RAW264.7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33048) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: TRF1 Overexpression Lysate (Adult Normal) [NBL1-16814] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRF1.](http://images.novusbio.com/fullsize/TRF1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16814-img0001.jpg "Western Blot: TRF1 Overexpression Lysate (Adult Normal) [NBL1-16814] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRF1.")
![ELISA: Human TRF-1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40193] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40193_human-trf-1-ready-to-use-elisa-kit-colorimetric-2110202415302997.png "ELISA: Human TRF-1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40193] - Standard Curve Reference")
![ELISA: Human TRF-1 ELISA Kit (Colorimetric) [NBP3-40194] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40194_human-trf-1-elisa-kit-colorimetric-2110202415393742.png "ELISA: Human TRF-1 ELISA Kit (Colorimetric) [NBP3-40194] - Standard Curve Reference")