POT1 Antibody

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Western Blot: POT1 Antibody [NB500-176] - Detection of POT1 in HeLa nuclear extracts (25 ug) using NB500-176(1:500). ECL detection 1 min.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2.25 mg/ml

POT1 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human POT1 protein sequence (between residues 200-300). [UniProt# Q9NUX5]
Localization
Nuclear
Predicted Species
Primate (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000 (ECL)
Application Notes
This POT1 antibody is use for Western blot, where a band at ~71.4 kDa is seen. Not recommended for ICC/IF. Nitrocellulose membranes may be necessary for optimal results, although this has not been completely determined.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
71.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC9)
POT1 Lysate (NBL1-14614)
Control Peptide
POT1 Peptide (NB500-176PEP)
Reviewed Applications
Read 1 Review rated 4
using
NB500-176 in the following application:

Publications
Read Publications using
NB500-176 in the following applications:

  • WB
    8 publications

Reactivity Notes

Human. Immunogen sequence has 95% homology to monkey, 82% homology to rat, and 72% homology to mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
2.25 mg/ml
Purity
Immunogen affinity purified

Alternate Names for POT1 Antibody

  • DKFZp586D211
  • HPOT1
  • POT1 protection of telomeres 1 homolog
  • POT1
  • POT1-like telomere end-binding protein
  • protection of telomeres 1 homolog (S. pombe)
  • protection of telomeres protein 1

Background

POT1 (protection of telomeres protein 1), a member of telombin family, is expressed ubiquitously and localizes to nucleus/chromosomal telomeres. It forms a component of telomerase RNP (ribonucleoprotein) complex which is essential for chromosome termini replication. POT1 participates in shelterin complex/telosome (contains TERF1, TERF2, TINF2, TERF2IP, ACD and POT1 - regulates telomeric length alongwith protecting telomers), telomerase holoenzyme complex (TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) and is also found complexed with TERF1, TINF2 and TNKS1. POT1 interacts with TNKS1 and can heterodimerize with ACD for enhancing telomere elongation via increase in telomerase processivity. POT1 implicates in double-stranded telomeric DNA-binding TRF1 complex which is essential to the regulation of telomere length by cis-inhibition of telomerase. POT1 acts as a single-stranded telomeric DNA-binding protein and thus behaves as a downstream effector of TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. POT1 functional alterations can lead to G-strand overhang loss, chromosomal-end fusions, chromosomal rearrangements, and rapid cell cycle arrest, ultimately leading to senescence and apoptosis. Accordingly, POT1 expression is altered in tumors and is specifically upregulated in therapeutic/radiation-resistant cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB

Publications for POT1 Antibody (NB500-176)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(8)
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Filter By Species
Human
(5)
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Showing Publications 1 - 9 of 9.
Publications using NB500-176 Applications Species
Swanson MJ, Baribault ME, Israel JN, Bae NS Telomere protein RAP1 levels are affected by cellular aging and oxidative stress. Biomed Rep 2016 Aug [PMID: 27446538] (WB) WB
Sishc BJ, Nelson CB, McKenna MJ et al. Telomeres and Telomerase in the Radiation Response: Implications for Instability, Reprograming, and Carcinogenesis. Front Oncol 2015 [PMID: 26636039] (WB) WB
Ci X, Li B, Ma X et al. Bortezomib-mediated down-regulation of telomerase and disruption of telomere homeostasis contributes to apoptosis of malignant cells. Oncotarget 2015 Nov 10 [PMID: 26472030] (WB) WB
Luo Z, Feng X, Wang H et al. Mir-23a induces telomere dysfunction and cellular senescence by inhibiting TRF2 expression. Aging Cell. 2015 Mar 06 [PMID: 25753893] (WB, Human) WB Human
Wojdyla L, Stone AL, Sethakorn N et al. T-oligo as an anticancer agent in colorectal cancer. Biochem. Biophys. Res. Commun. 3/12/2014 [PMID: 24632202] (WB, Human) WB Human
Kamranvar SA, Chen X, Masucci MG. Telomere dysfunction and activation of alternative lengthening of telomeres in B-lymphocytes infected by Epstein-Barr virus. Oncogene 2013 May 27 [PMID: 23708666] (WB, Human) WB Human
Jullien L, Mestre M, Roux P, Gire V. Eroded human telomeres are more prone to remain uncapped and to trigger a G2 checkpoint response Nucleic Acids Res 2012 Nov 27 [PMID: 23193277] (WB, Human) WB Human
Yang, Q et al. Functional diversity of human protection of telomeres 1 isoforms in telomere protection and cellular senescence. Cancer Res;67(24):11677-86. 2007 Dec 15. [PMID: 18089797]
Chen, L et al. Telomere maintenance through spatial control of telomeric proteins. Mol Cell Biol;27(16):5898-909. 2007 Aug. [PMID: 17562870] (WB, Human) WB Human

Review for POT1 Antibody (NB500-176) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB500-176:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 09/23/2009
View

Summary

ApplicationWestern Blot
Sample TestedHuman lympoblast cells, Sample Amount: 35 ug
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% BSA/TBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/600, Incubation Dilution Buffer: 5% BSA TBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Licor rabbit pAb, Secondary Ab Dilution Ratio: 1/15000

Details

Detection NotesDetection Method: Oddessey, Specific Bands: 75 kDa, Non-Specific Bands: 50 kDa

Product General Protocols

View specific protocols for POT1 Antibody (NB500-176): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for POT1 Antibody (NB500-176) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-176AF405
Alexa Fluor 488 Labeled NB500-176AF488
Alexa Fluor 647 Labeled NB500-176AF647
Alexa Fluor 700 Labeled NB500-176AF700
Biotin Labeled NB500-176B
DyLight 405 Labeled NB500-176V
DyLight 488 Labeled NB500-176G
DyLight 550 Labeled NB500-176R
DyLight 650 Labeled NB500-176C
HRP Labeled NB500-176H

Additional Array Products

Bioinformatics Tool for POT1 Antibody (NB500-176)

Discover related pathways, diseases and genes to POT1 Antibody (NB500-176). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for POT1 Antibody (NB500-176)

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Pathways for POT1 Antibody (NB500-176)

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Research Areas for POT1 Antibody (NB500-176)

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Blogs on POT1.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

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Recent Reviews

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5
0
4
1
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2
0
1
0

 
Anonymous
09/23/2009
Application: WB
Species: Human

Bioinformatics

Gene Symbol POT1
Entrez
Uniprot