Pin1 Antibody (257417) [Unconjugated]

Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line, U2OS human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse Pin1 Monoclonal Antibody (Catalog # MAB2294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pin1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Pin1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human/Mouse Pin1 Monoclonal Antibody (Catalog # MAB2294) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Pin1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/Mouse Pin1 Monoclonal Antibody (Catalog # MAB2294) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP, ICC/IF
• Clone: 257417
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Pin1 Antibody (257417) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Pin1
  Ala2-Glu163
  Accession # Q13526
• Specificity: Detects human and mouse Pin1 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIN1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 2-3 ug/500 ug cell lysate
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pin1 Antibody (257417) [Unconjugated]

• dod
• EC 5.2.1.8
• peptidylprolyl cis/trans isomerase, NIMA-interacting 1
• peptidyl-prolyl cis/trans isomerase, NIMA-interacting
• peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
• Peptidyl-prolyl cis-trans isomerase Pin1
• Pin1
• PPIase Pin1
• prolyl isomerase
• protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1
• protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1
• Rotamase Pin1
• UBL5

Background

Pin1 is a peptidyl-prolyl isomerase (PPI) that targets phosphorylated Ser or Thr residues followed by a Pro (S/T-P). Isomerization of phosphorylated Ser or Thr residues may alter protein confirmation and, subsequently, modify activity. Pin1 is overexpressed in many human breast cancers, and has been shown to modify numerous proteins including p53,NF-kappa B, c-Jun, cyclin D1, and beta -catenin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pin1 Antibody (MAB2294)(7)

Publications using MAB2294

• YT Wang, WY Hsiao, SW Wang The fission yeast Pin1 peptidyl-prolyl isomerase promotes dissociation of Sty1 MAPK from RNA polymerase II and recruits Ssu72 phosphatase to facilitate oxidative stress induced transcription Nucleic Acids Research, 2021-01-25;49(2):805-817. 2021-01-25 [PMID: 33410907] (Western Blot, Human)
• ZJ Shen, J Hu, V Kashi, YA Bochkov, JE Gern, JS Malter TLR-7 Stress Signaling in Differentiating and Mature Eosinophils Is Mediated by the Prolyl Isomerase Pin1 J. Immunol., 2018-11-05;0(0):. 2018-11-05 [PMID: 30397031] (Immunoprecipitation, Mouse)
• Ghosh A, Saminathan H, Kanthasamy A, Anantharam V, Jin H, Sondarva G, Harischandra D, Qian Z, Rana A, Kanthasamy A The peptidyl-prolyl isomerase Pin1 up-regulation and proapoptotic function in dopaminergic neurons: relevance to the pathogenesis of Parkinson disease. J Biol Chem, 2013-06-10;288(30):21955-71. 2013-06-10 [PMID: 23754278] (Western Blot, Human)
• Nishi M, Akutsu H, Masui S A distinct role for Pin1 in the induction and maintenance of pluripotency. J. Biol. Chem., 2011-02-04;286(13):11593-603. 2011-02-04 [PMID: 21296877] (Immunoprecipitation, Human)
• Brenkman AB, de Keizer PL, van den Broek NJ, van der Groep P, van Diest PJ, van der Horst A, Smits AM, Burgering BM The peptidyl-isomerase Pin1 regulates p27kip1 expression through inhibition of Forkhead box O tumor suppressors. Cancer Res., 2008-09-15;68(18):7597-605. 2008-09-15 [PMID: 18794148] (ICC, IHC-P, Human)
• Liu T, Huang Y, Likhotvorik RI, Keshvara L, Hoyt DG Protein Never in Mitosis Gene A Interacting-1 (PIN1) regulates degradation of inducible nitric oxide synthase in endothelial cells. Am. J. Physiol., Cell Physiol., 2008-07-23;295(3):C819-27. 2008-07-23 [PMID: 18650263] (Immunoprecipitation, Western Blot, Mouse)
• Shen ZJ, Esnault S, Rosenthal LA, Szakaly RJ, Sorkness RL, Westmark PR, Sandor M, Malter JS Pin1 regulates TGF-beta1 production by activated human and murine eosinophils and contributes to allergic lung fibrosis. J. Clin. Invest., 2008-02-01;118(2):479-90. 2008-02-01 [PMID: 18188456] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: PIN1
• Uniprot:
  • Q13526()