TERT Antibody (2C4)

Images

 
Western Blot: TERT Antibody (2C4) [NB100-317] - 1:500 dilution on MJ90 cells.
Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317] - Human Pancreatic carcinoma showing strong staining of tumor cells.
Flow Cytometry: TERT Antibody (2C4) [NB100-317] - An intracellular stain was performed on Jurkat cells with TERT Antibody (2C4) NB100-317 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more
Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317] - Normal human pancreas showing moderate staining of exocrine cells and a subset of islets of Langerhans.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
2C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TERT Antibody (2C4) Summary

Immunogen
Full-length recombinant human Telomerase reverse transcriptase (TERT) from insect cells. [Uniprot: O14746].
Localization
Nuclear
Marker
Embryonic Stem Cell Marker
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
TERT
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 1:10 - 1:500
Application Notes
In Western blot, this antibody recognizes a band at ~127 kDa. An additional background band may be seen at ~110 kDa. Note that the isotype is IgM and the appropriate secondary should be used. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26725521).
Theoretical MW
127 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
TERT Overexpression Lysate
Publications
Read Publications using
NB100-317 in the following applications:

  • 1 publication
  • 2 publications
  • IHC
    5 publications
  • 3 publications
  • IP
    1 publication
  • WB
    7 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgM purified

Alternate Names for TERT Antibody (2C4)

  • EST2Telomerase-associated protein 2
  • hEST2
  • hTERT
  • TCS1EC 2.7.7.49
  • Telomerase catalytic subunit
  • telomerase reverse transcriptase
  • TERT
  • TP2HEST2
  • TRTEC 2.7.7

Background

Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication that is active in progenitor as well as cancer cells, and stays inactive or show very low activity in normal somatic cells. TERT is the catalytic component of the holoenzyme complex (TERT, DKC1, WDR79/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) that implicates in telomeres elongation by acting as a reverse transcriptase adding simple sequence repeats to chromosome ends by copying a template sequence within RNA component of the enzyme. For executing its versatile functions, TERT can interact with HSP90A, PTGES3, HSPA1A, RAN, XPO1, PTPN11, NCL, SMARCA4, MCRS1, PIF1, PML and GNL3L. TERT modulates Wnt signaling and plays important roles in aging and anti-apoptosis. Telomerase activation has been implicated in cell immortalization/ carcinogenesis and defects in TERT are associated with susceptibilty to aplastic anemia, coronary artery disease (CAD), dyskeratosis congenita autosomal dominant type 2 (DKCA2), pulmonary fibrosis, and/or bone marrow failure, telomere-related, type 1 (PFBMFT1), dyskeratosis congenita autosomal recessive type 4 (DKCB4), susceptibility to pulmonary fibrosis idiopathic (IPF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-317
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP

Publications for TERT Antibody (NB100-317)(19)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ChIP
(1)
ICC/IF
(2)
IHC
(5)
IHC-P
(3)
IP
(1)
WB
(7)
All Applications
Filter By Species
Human
(11)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB100-317 Applications Species
Kokubun T, Saitoh SI, Miura S et al. Telomerase Plays a Pivotal Role in Collateral Growth Under Ischemia by Suppressing Age-Induced Oxidative Stress, Expression of p53, and Pro-Apoptotic Proteins Int Heart J May 30 2019 [PMID: 31105157] (WB, Mouse) WB Mouse
Wei, J;Yang, Q;Shi, J;Shi, B;Ji, M;Hou, P; Increased expression of NAF1 contributes to malignant phenotypes of glioma cells through promoting protein synthesis and associates with poor patient survival Oncogenesis Apr 1 2019 [PMID: 30936423] (ChIP, Human) ChIP Human
Giacalone JC, Miller MJ, Workalemahu G et al. Generation of an immortalized human choroid endothelial cell line (iChEC-1) using an endothelial cell specific promoter Microvasc. Res. Dec 18 2018 [PMID: 30571950] (ICC/IF, Human) ICC/IF Human
Lee SJ, Shin SP, Lee SH et al. Phase I trial of intravenous Ad5CRT in patients with liver metastasis of gastrointestinal cancers. Cancer Gene Ther. Nov 5 2018 [PMID: 30393375] (IHC, Human) IHC Human
Margalef P, Kotsantis P, Borel V et al. Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe. Cell 2017 Dec 27 [PMID: 29290468] (WB, Mouse) WB Mouse
Biron-Shental T, Liberman M, Elbaz M, Laish I. Telomere homeostasis in placentas from pregnancies with uncontrolled diabetes. Placenta. May 19 2016 (IHC, Human) IHC Human
Chen J, Yao ZX, Chen JS et al. TGF-beta/beta2-spectrin/CTCF-regulated tumor suppression in human stem cell disorder Beckwith-Wiedemann syndrome. J. Clin. Invest. 2016 Feb 01 [PMID: 26784546] (IHC-P, Human, Mouse) IHC-P Human, Mouse
Wu XQ, Yang Y, Li WX et al. Telomerase reverse transcriptase acts in a feedback loop with NF-kB pathway to regulate macrophage polarization in alcoholic liver disease. Sci Rep. 2016 Jan 04 [PMID: 26725521] (WB, ICC/IF, IHC-P, Mouse) WB, ICC/IF, IHC-P Mouse
Lotfi RA, El Zawahry KM, Kamar ZA, Hashem Z. Effects of smoking on human telomerase reverse transcriptase expression in the skin. Int. J. Dermatol. 3/6/2014 [PMID: 24601896] (IHC-P, Human) IHC-P Human
Dosset M, Godet Y, Vauchy C et al. Universal cancer peptide-based therapeutic vaccine breaks tolerance against telomerase and eradicates established tumor Clin Cancer Res 2012 Oct 2 [PMID: 23032748] (WB, Mouse) WB Mouse
Show All 19 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TERT Antibody (NB100-317). (Showing 1 - 1 of 1 FAQs).

  1. What part of hTERT is recognised by NB100-317?
    • The immunogen used to generate our telomerase reverse transcriptase antibody with catalogue number NB100-317 is full-length recombinant human telomerase reverse transcriptase from insect cells [UniProt# O14746]. We do not epitope map our antibodies and so I am unfortunately unable to give you any further information regarding the binding of this antibody to its target.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on TERT.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
By Jamshed Arslan Pharm.D. Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amyloid beta (a polypepti...  Read full blog post.

Reversing Cancer with Telomerase Reverse Transcriptase (TERT)
Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication. It is a useful marker as it is active only in progenitor and most cancer cells, but inactive or (active at very low activit...  Read full blog post.

Featured Product Citation using Novus' hTERT Antibody
In December 2009, researchers at the University of Southern California cited using Novus’ mouse monoclonal Telomerase Reverse Transcriptase Antibody (cat# NB100-317) [PMID: 19923445]. Specifically, Novus’ hTERT antibody was used for immunofluorescent...  Read full blog post.

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Bioinformatics

Gene Symbol TERT
Entrez
Uniprot