Western Blot: TERT Antibody (2C4) [NB100-317] - Increased levels of IFI16 protein in WI-38 cells reduce hTERT levels and inhibit telomerase activity. Total protein extracts prepared from WI-38 cells, either transfected ...read more
Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317] - Human Pancreatic carcinoma showing strong staining of tumor cells.
Flow Cytometry: TERT Antibody (2C4) [NB100-317] - An intracellular stain was performed on Jurkat cells with TERT Antibody (2C4) NB100-317 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more
Western Blot: TERT Antibody (2C4) [NB100-317] - 1:500 dilution on MJ90 cells.
Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317] - Normal human pancreas showing moderate staining of exocrine cells and a subset of islets of Langerhans.
In Western blot, this antibody recognizes a band at ~127 kDa. An additional background band may be seen at ~110 kDa. Note that the isotype is IgM and the appropriate secondary should be used. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26725521). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30936423).
127 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Rat reactivity reported in scientific literature (Mustofa et al).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Alternate Names for TERT Antibody (2C4)
EST2Telomerase-associated protein 2
Telomerase catalytic subunit
telomerase reverse transcriptase
Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication that is active in progenitor as well as cancer cells, and stays inactive or show very low activity in normal somatic cells. TERT is the catalytic component of the holoenzyme complex (TERT, DKC1, WDR79/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) that implicates in telomeres elongation by acting as a reverse transcriptase adding simple sequence repeats to chromosome ends by copying a template sequence within RNA component of the enzyme. For executing its versatile functions, TERT can interact with HSP90A, PTGES3, HSPA1A, RAN, XPO1, PTPN11, NCL, SMARCA4, MCRS1, PIF1, PML and GNL3L. TERT modulates Wnt signaling and plays important roles in aging and anti-apoptosis. Telomerase activation has been implicated in cell immortalization/ carcinogenesis and defects in TERT are associated with susceptibilty to aplastic anemia, coronary artery disease (CAD), dyskeratosis congenita autosomal dominant type 2 (DKCA2), pulmonary fibrosis, and/or bone marrow failure, telomere-related, type 1 (PFBMFT1), dyskeratosis congenita autosomal recessive type 4 (DKCB4), susceptibility to pulmonary fibrosis idiopathic (IPF).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Mustofa M, Suyatna F, Sadikin M et al. Soybean extract increases telomerase reverse transcriptase protein expression in pancreatic beta-cells of diabetes mellitus-induced rats Med J Indones 2019 Oct 04 (IHC, Rat)
FAQs for TERT Antibody (NB100-317). (Showing 1 - 1 of 1 FAQs).
What part of hTERT is recognised by NB100-317?
The immunogen used to generate our telomerase reverse transcriptase antibody with catalogue number NB100-317 is full-length recombinant human telomerase reverse transcriptase from insect cells [UniProt# O14746]. We do not epitope map our antibodies and so I am unfortunately unable to give you any further information regarding the binding of this antibody to its target.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.