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TERT Antibody (2C4)

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Western Blot: TERT Antibody (2C4) [NB100-317] - Increased levels of IFI16 protein in WI-38 cells reduce hTERT levels and inhibit telomerase activity. Total protein extracts prepared from WI-38 cells, either transfected ...read more
Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317] - Human Pancreatic carcinoma showing strong staining of tumor cells.
Flow Cytometry: TERT Antibody (2C4) [NB100-317] - An intracellular stain was performed on A431 cells with TERT Antibody [2C4] NB100-317 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more
Western Blot: TERT Antibody (2C4) [NB100-317] - 1:500 dilution on MJ90 cells.
Immunohistochemistry-Paraffin: TERT Antibody (2C4) [NB100-317] - Normal human pancreas showing moderate staining of exocrine cells and a subset of islets of Langerhans.
Flow Cytometry: TERT Antibody (2C4) [NB100-317] - An intracellular stain was performed on Jurkat cells with TERT Antibody (2C4) NB100-317 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more
Co-localization of HIF-2 alpha with HIF-1 beta and RNAPII. (a) Immunofluorescence detecting indicated proteins using antibodies labelled with Alexa Fluor 555 (red, pseudocolour) and Alexa Fluor 488 (green, ...read more
ATM is activated by spermidine in GM00637 cells.GM00637 cells with or without KU55933 pretreatment (a,b), and GM05849 cells (c) were exposed to 50 μM spermidine or CCCP, followed by immunofluorescence analyses of ...read more
MiR‐200c inhibition promoted cardiac hypertrophy via regulation of apoptosis. (A,B) Cell apoptosis was detected by flow cytometric analysis, and the percentage of apoptotic cells transfected with miR‐200c mimics or ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, Flow, ICC/IF, IHC, IP, ChIP
Clone
2C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price

TERT Antibody (2C4) Summary

Immunogen
Full-length recombinant human Telomerase reverse transcriptase (TERT) from insect cells. [Uniprot: O14746].
Localization
Nuclear
Marker
Embryonic Stem Cell Marker
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
TERT
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 30936423)
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200. Use reported in scientific literature (PMID 26725521)
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
Application Notes
In Western blot, this antibody recognizes a band at ~127 kDa. An additional background band may be seen at ~110 kDa. Note that the isotype is IgM and the appropriate secondary should be used.
Theoretical MW
127 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-317 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (Mustofa et al).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgM purified

Alternate Names for TERT Antibody (2C4)

  • EST2Telomerase-associated protein 2
  • hEST2
  • hTERT
  • TCS1EC 2.7.7.49
  • Telomerase catalytic subunit
  • telomerase reverse transcriptase
  • TERT
  • TP2HEST2
  • TRTEC 2.7.7

Background

Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication that is active in progenitor as well as cancer cells, and stays inactive or show very low activity in normal somatic cells. TERT is the catalytic component of the holoenzyme complex (TERT, DKC1, WDR79/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) that implicates in telomeres elongation by acting as a reverse transcriptase adding simple sequence repeats to chromosome ends by copying a template sequence within RNA component of the enzyme. For executing its versatile functions, TERT can interact with HSP90A , PTGES3, HSPA1A, RAN , XPO1, PTPN11, NCL, SMARCA4, MCRS1, PIF1, PML and GNL3L. TERT modulates Wnt signaling and plays important roles in aging and anti-apoptosis. Telomerase activation has been implicated in cell immortalization/ carcinogenesis and defects in TERT are associated with susceptibilty to aplastic anemia, coronary artery disease (CAD), dyskeratosis congenita autosomal dominant type 2 (DKCA2), pulmonary fibrosis, and/or bone marrow failure, telomere-related, type 1 (PFBMFT1), dyskeratosis congenita autosomal recessive type 4 (DKCB4), susceptibility to pulmonary fibrosis idiopathic (IPF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TERT Antibody (NB100-317)(25)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: Chemotaxis, ICC/IF, IF/IHC, IHC, IHC-P, IP, WB.


Filter By Application
Chemotaxis
(1)
ICC/IF
(3)
IF/IHC
(6)
IHC
(1)
IHC-P
(4)
IP
(1)
WB
(7)
All Applications
Filter By Species
Human
(13)
Mouse
(5)
Rat
(1)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB100-317 Applications Species
Liu T, Long Q, Li L et al. The NRF2-dependent transcriptional axis, XRCC5/hTERT, drives tumor progression and 5-Fu insensitivity in hepatocellular carcinoma Molecular Therapy - Oncolytics 2021-12-01 [PMID: 35071747] (IHC-P, Human) IHC-P Human
Collins DP, Osborn MJ, Steer CJ. et al. Differentiation of immortalized human multi-lineage progenitor to alveolar type 2-like cells: angiotensin-converting enzyme 2 expression and binding of severe acute respiratory syndrome coronavirus 2 spike and spike 1 proteins Cytotherapy 2021-08-01 [PMID: 34551876] (ICC/IF, Human)

Details:
Citation using the Azide and BSA Free format of this antibody.
ICC/IF Human
Del Moral-Hernández O, Hernández-Sotelo D, Alarcón-Romero LDC, et al. TOP2A/MCM2, p16INK4a, and cyclin E1 expression in liquid-based cytology: a biomarkers panel for progression risk of cervical premalignant lesions BMC cancer 2021-01-07 [PMID: 33413211]
Kubo C, Ogawa M, Uehara N, Katakura Y. Fisetin Promotes Hair Growth by Augmenting TERT Expression Frontiers in Cell and Developmental Biology 2020-10-15 [PMID: 33178686] (IHC) IHC
Collins D, Hapke J, Aravalli R, Steer C In vitro Differentiation of TERT-Transfected Multi-Lineage Progenitor Cells (MLPC) into Immortalized Hepatocyte-Like Cells HMER 2020-06-01 [PMID: 32607015]
Kokubun T, Saitoh SI, Miura S et al. Telomerase Plays a Pivotal Role in Collateral Growth Under Ischemia by Suppressing Age-Induced Oxidative Stress, Expression of p53, and Pro-Apoptotic Proteins Int Heart J 2019-05-30 [PMID: 31105157] (WB, Mouse) WB Mouse
Wei, J;Yang, Q;Shi, J;Shi, B;Ji, M;Hou, P; Increased expression of NAF1 contributes to malignant phenotypes of glioma cells through promoting protein synthesis and associates with poor patient survival Oncogenesis 2019-04-01 [PMID: 30936423] (Chemotaxis, Human) Chemotaxis Human
Lee SJ, Shin SP, Lee SH et al. Phase I trial of intravenous Ad5CRT in patients with liver metastasis of gastrointestinal cancers. Cancer Gene Ther. 2018-11-05 [PMID: 30393375] (IF/IHC, Human) IF/IHC Human
Margalef P, Kotsantis P, Borel V et al. Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe. Cell 2017-12-27 [PMID: 29290468] (WB, Mouse) WB Mouse
Telomere homeostasis in placentas from pregnancies with uncontrolled diabetes. Biron-Shental T, Liberman M, Elbaz M Placenta [PMID: 27452433] (IF/IHC, Human) IF/IHC Human
Show All 25 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for TERT Antibody (NB100-317). (Showing 1 - 1 of 1 FAQs).

  1. What part of hTERT is recognised by NB100-317?
    • The immunogen used to generate our telomerase reverse transcriptase antibody with catalogue number NB100-317 is full-length recombinant human telomerase reverse transcriptase from insect cells [UniProt# O14746]. We do not epitope map our antibodies and so I am unfortunately unable to give you any further information regarding the binding of this antibody to its target.

Secondary Antibodies

 

Isotype Controls

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Research Areas for TERT Antibody (NB100-317)

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Blogs on TERT.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.

Reversing Cancer with Telomerase Reverse Transcriptase (TERT)
Telomerase reverse transcriptase (TERT) is a ribonucleoprotein enzyme essential for eukaryotic chromosomal termini replication. It is a useful marker as it is active only in progenitor and most cancer cells, but inactive or (active at very low activit...  Read full blog post.

Featured Product Citation using Novus' hTERT Antibody
In December 2009, researchers at the University of Southern California cited using Novus’ mouse monoclonal Telomerase Reverse Transcriptase Antibody (cat# NB100-317) [PMID: 19923445]. Specifically, Novus’ hTERT antibody was used for immunofluorescent ...  Read full blog post.

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Bioinformatics

Gene Symbol TERT
Entrez
Uniprot