TRF-2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: TRF-2 Antibody [NB110-57130] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with antibody at a 1:200 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with Dylight 550 (Red). Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.

Simple Western: TRF-2 Antibody [NB110-57130] - Lane view shows a specific band for TRF2 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: TRF-2 Antibody [NB110-57130] - Analysis of HeLa whole cell lysate (A), HeLa nuclear cell lysate (B), k562 cell lysate (C), HepG2 cell lysate (D), NIH/3T3 cell lysate (E), CHO cell lysate (F), PC12 cell lysate (G), and Cos7 cell lysate (H) using antibody at a concentration of 2 ug/mL.

Immunohistochemistry-Paraffin: TRF-2 Antibody [NB110-57130] - Analysis in xenografted human breast cancer tissue using DAB with hematoxylin counterstain.

Immunohistochemistry-Paraffin: TRF-2 Antibody [NB110-57130] - Analysis of FFPE human breast cancer tissue with rabbit polyclonal TRF2 antibody at a dilution of 1:200. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. This TRF2 antibody generated an expected nuclear signal in all the cancer cells and the stromal cells. In the tested section, only a subset of myoepithelial cells showed positivity for this protein.

Immunocytochemistry/Immunofluorescence: TRF-2 Antibody [NB110-57130] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TRF-2 Antibody NB110-57130 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: TRF-2 Antibody [NB110-57130] - An intracellular stain was performed on HeLa cells with TRF-2 Antibody NB110-57130 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Chromatin Immunoprecipitation: TRF-2 Antibody [NB110-57130] - Analysis in mouse. Titrated TRF2 antibody to determine concentration required for ChIP experiment. ChIP image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: TRF-2 Antibody [NB110-57130] - RNAi-mediated depletion of human separase (ESPL1) induces TIFs. Control scrambled siRNA- (control) and ESPL1 siRNA-treated fibroblasts stained with anti-p53-binding protein 1 (53BP1; green) and anti-TRF2 (red). It is noteworthy that in ESPL1 siRNA-treated cells, 53BP1 signals frequently overlap with TRF2 signals marking the TIFs. Scale bar, 5 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms10405), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: TRF-2 Antibody [NB110-57130] - Analysis of FFPE human breast cancer tissue with rabbit polyclonal TRF2 antibody at 1:200 dilution. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. This TRF2 antibody generated an expected nuclear signal in all the cancer cells and the stromal cells. In the tested section, only a subset of myoepithelial cells showed positivity for this protein.

Flow Cytometry: TRF-2 Antibody [NB110-57130] - An intracellular stain was performed on HeLa cells with TRF-2 Antibody NB110-57130AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

• Reactivity: Hu, Mu, Rt, ChHa, Pm
• Applications: WB, Simple Western, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TRF-2 Antibody - BSA Free Summary

• Immunogen: This TRF-2 Antibody was developed against Baculovirus purified TRF2 protein.
• Localization: Nuclear
• Marker: Telomeres marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TERF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Dot Blot
  • ELISA
  • Flow Cytometry 1-5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • Knockdown Validated
  • Simple Western 1:25
  • Western Blot 1:2000 - 1:5000
• Application Notes: This TRF2 antibody is useful for ChIP, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band at approx. 56 kDa is seen. Immunoprecipitation was reported in scientific literature. Use in Knockdown Validated, and Dot blot reported in scientific literature (PMID: 31026066). Use in ELISA reported in scientific literature (PMID: 31575660). In ICC/IF, nuclear staining was observed in HeLa cells. In IHC, nuclear staining was observed in xenografted human breast cancer tissue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 59.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Nuclear Cell Lysate
  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRF-2 Antibody - BSA Free

• Telomeric DNA-binding protein
• telomeric repeat binding factor 2
• Telomeric repeat binding protein 2
• telomeric repeat-binding factor 2
• TERF2
• TRBF2
• TRF2
• TRF-2
• TTAGGG repeat binding factor 2
• TTAGGG repeat-binding factor 2

Background

Originally discovered as part of shelterin complex, telomeric repeat-binding factor 2 (TRF2, also called TERF2 or TRB2) is a ubiquitously expressed nuclear protein (55-60 kDa) involved in telomere homeostasis. TRF2 contains an N-terminal GAR domain, a central TRFH dimerization domain, and a C-terminal SAND/MYB-type DNA binding domain. Trf2 RNA has 10 exons and alternative splicing in rodents produces a truncated form, TRF2-S, which lacks the DNA binding domain and nuclear localization signal (NLS) (1).

Both TRF2 and TRF1 bind to telomeric double stranded 5'-TTAGGG-3' DNA repeats, then recruit RAP1, TIN2, TPP1, and POT1 for the assembly of the shelterin complex. The telomeric association of TRF2 is greatly increased in the S phase of the cell cycle (2). Loss of TRF2 leads to telomere shortening, the DNA damage response, chromosomal instability, and replicative senescence. Interestingly, the contribution of TRF2 to telomere shortening via a telomerase-independent mechanism has also been reported (3). In conjunction with the exonuclease, Apollo, TRF2 protects telomeres during replication and negatively regulates the accumulation of DNA topoisomerase (TOP1, TOP2A and TOP2B).

TRF2 has been implicated in cancer, shown to be a major oncogene in telomerase-deficient mice. A link to Werner syndrome, a premature aging disease caused by the loss of WRN, has been reported based on TRF2 recruitment of WRN for processing of telomeric DNA (4). TRF2 expression is increased during human embryonic stem cell differentiation and has been shown to interact with Repressor Element-1 Silencing Transcription Factor (REST), protecting it from proteasomal degradation (5).

References

1. Grammatikakis, I., Zhang, P., Mattson, M. P., & Gorospe, M. (2016). The long and the short of TRF2 in neurogenesis. Cell cycle (Georgetown, Tex.), 15(22), 3026-3032. PMID: 27565210

2. Li, F., Kim, H., Ji, Z., Zhang, T., Chen, B., Ge, Y., Hu, Y., Feng, X., Han, X., Xu, H., Zhang, Y., Yu, H., Liu, D., Ma, W., & Songyang, Z. (2018). The BUB3-BUB1 Complex Promotes Telomere DNA Replication. Molecular cell, 70(3), 395-407. PMID: 29727616

3. Ancelin, K., Brunori, M., Bauwens, S., Koering, C. E., Brun, C., Ricoul, M., Pommier, J. P., Sabatier, L., & Gilson, E. (2002). Targeting assay to study the cis functions of human telomeric proteins: evidence for inhibition of telomerase by TRF1 and for activation of telomere degradation by TRF2. Molecular and cellular biology, 22(10), 3474-3487. PMID: 11971978

4. Machwe A, Xiao L, & Orren DK. (2004) TRF2 recruits the Werner syndrome (WRN) exonuclease for processing of telomeric DNA. Oncogene. 23(1):149-56. PMID: 14712220.

5. Diotti, R., & Loayza, D. (2011). Shelterin complex and associated factors at human telomeres. Nucleus (Austin, Tex.), 2(2), 119-135. PMID: 21738835

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRF-2 Antibody (NB110-57130)(113)

Publications using NB110-57130

• Jiang H, Kong N, Liu Z et al. Human Endonuclease ANKLE1 Localizes at the Midbody and Processes Chromatin Bridges to Prevent DNA Damage and cGAS-STING Activation Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023-02-24 [PMID: 36825683] (ICC/IF, Human)
  
  Details:
  Dilution used in ICC/IF 1:1000
• Stock AJ, McDevitt RA, Puligilla C et al. Aberrant expression and localization of the RAP1 shelterin protein contribute to age-related phenotypes PLoS genetics 2022-11-01 [PMID: 36441670] (WB, Mouse)
• Iachettini S, Ciccarone F, Maresca C et al. The telomeric protein TERF2/TRF2 impairs HMGB1-driven autophagy Autophagy 2022-10-30 [PMID: 36310382] (ICC/IF, Human)
• Kaminski N, Wondisford AR, Kwon Y et al. RAD51AP1 regulates ALT-HDR through chromatin-directed homeostasis of TERRA Molecular cell 2022-10-14 [PMID: 36265488]
• Yadav T, Zhang JM, Ouyang J et al. TERRA and RAD51AP1 promote alternative lengthening of telomeres through an R- to D-loop switch Molecular cell 2022-10-11 [PMID: 36265486] (ICC/IF, PLA, Human)
• Guh CY, Shen HJ, Chen LW et al. XPF activates break-induced telomere synthesis Nature communications 2022-10-02 [PMID: 36184605] (FISH)
  
  Details:
  Dilution used in 1:200
• Mendez-Bermudez A, Lototska L, Pousse M et al. Selective pericentromeric heterochromatin dismantling caused by TP53 activation during senescence Nucleic acids research 2022-07-22 [PMID: 35819196] (WB, Human)
• Tanno N, Takemoto K, Takada-Horisawa Y et al. FBXO47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis iScience 2022-03-01 [PMID: 35310947] (WB, Mouse)
• Dinami R, Petti E, Porru M et al. TRF2 cooperates with CTCF for controlling the oncomiR-193b-3p in colorectal cancer Cancer letters 2022-02-28 [PMID: 35240232] (Chemotaxis, Human)
• Frank L, Rademacher A, Mucke N et al. ALT-FISH quantifies alternative lengthening of telomeres activity by imaging of single-stranded repeats Nucleic acids research 2022-02-21 [PMID: 35188570]
• Kroustallaki P Functions of SMUG1 and NEIL3 in telomere homeostasis Thesis 2020-01-01
• SAnchez-VAzquez R, MartInez P, Blasco MA AKT-dependent signaling of extracellular cues through telomeres impact on tumorigenesis PLoS genetics 2021-03-01 [PMID: 33690611] (WB, Human)
• Sharma S, Mukherjee AK, Roy SS et al. Human Telomerase Expression is under Direct Transcriptional Control of the Telomere-binding-factor TRF2 bioRxiv 2020-01-01
• Sukumar A, Williams CL, Jones CY et al. The DNA end-binding protein Ku associates with human telomeres primarily via protein-protein interactions bioRxiv 2019-12-12 (IP, Human)
• Zhang K, Tarczykowska A, Gupta DK et al. The TERB1 MYB domain suppresses telomere erosion in meiotic prophase I Cell reports 2022-01-25 [PMID: 35081355] (ICC/IF, Mouse)
• Jack A, Kim Y, Strom AR et al. Compartmentalization of telomeres through DNA-scaffolded phase separation Developmental cell 2022-01-24 [PMID: 35077681] (WB, Human)
• De Sadeleer LJ, McDonough JE, Schupp JC Et al. Lung Microenvironments and Disease Progression in Fibrotic Hypersensitivity Pneumonitis American journal of respiratory and critical care medicine 2021-11-01 [PMID: 34724391]
• Mangosh TL, Grabowska MM, Taylor DJ SLX4IP N-terminus dictates telomeric localization in ALT-like castration-resistant prostate cancer cell lines The Prostate 2021-11-01 [PMID: 34492133]
• Sharma S, Mukherjee AK, Roy SS et al. Human telomerase is directly regulated by non-telomeric TRF2-G-quadruplex interaction Cell reports 2021-05-18 [PMID: 34010660] (WB, Human)
• Bauwens S, Lototska L, Koundrioukoff S et al. The Telomeric Protein TRF2 Regulates Replication Origin Activity within Pericentromeric Heterochromatin Life 2021-03-24 [PMID: 33804994] (WB)
• Kim C, Sung S, Kim JS et al. Telomeres reforged with non-telomeric sequences in mouse embryonic stem cells Nature communications 2021-02-17 [PMID: 33597549] (ICC/IF, Mouse)
• Kosebent EG, Ozturk S The spatiotemporal expression of TERT and telomere repeat binding proteins in the postnatal mouse testes Andrologia 2021-02-05 [PMID: 33544428]
• Zhang JM, Genois MM, Ouyang J, et al. Alternative lengthening of telomeres is a self-perpetuating process in ALT-associated PML bodies Molecular cell 2021-01-08 [PMID: 33453166]
• Lamm N, Read MN, Nobis M et al. Nuclear F-actin counteracts nuclear deformation and promotes fork repair during replication stress Nature cell biology 2020-12-01 [PMID: 33257806]
• Mangosh TL SLX4 Interacting Protein (SLX4IP): A Vital Primer for Alternative Lengthening of Telomere (ALT)-like Processes Promoting Replicative Immortality in Castration-resistant Prostate Cancer with Androgen Receptor Loss Thesis 1905-07-13 (WB, Human)
• Viceconte N, Loriot A, Lona Abreu P et al. PAR-TERRA is the main contributor to telomeric repeat-containing RNA transcripts in normal and cancer mouse cells RNA 2020-10-30 [PMID: 33127860] (WB, Mouse)
• Pinzaru AM, Kareh M, Lamm N et al. Met Mutation is a Potential Therapeutic Target for Advanced Endometrial Cancer Genes Dev 2020-09-28 [PMID: 33122293] (WB, Human)
• Querido E, Sfeir A, Chartrand P ALKBH5 inhibited cell proliferation and sensitized bladder cancer cells to cisplatin by m6A-CK2 alpha mediated glycolysis STAR Protoc 2020-10-01 [PMID: 33111129] (ICC/IF, Human)
• Hoang SM, Kaminski N, Bhargava R et al. Regulation of ALT-associated homology-directed repair by polyADP-ribosylation Nat Struct Mol Biol 2020-10-12 [PMID: 33046907]
• Uysal F, Kosebent EG, Toru HS, Ozturk S Decreased expression of TERT and telomeric proteins as human ovaries age may cause telomere shortening J. Assist. Reprod. Genet. 2020-08-27 [PMID: 32856217]
• Segura-Bayona S, Villamor-PayA M, Attolini CS et al. Tousled-Like Kinases Suppress Innate Immune Signaling Triggered by Alternative Lengthening of Telomeres Cell Rep 2020-08-04 [PMID: 32755577]
• Awad A, Glousker G, Lamm N et al. Full length RTEL1 is required for the elongation of the single-stranded telomeric overhang by telomerase Nucleic Acids Res. 2020-06-15 [PMID: 32542379] (ICC/IF, Human)
• Laprade H, Querido E, Smith MJ et al. Single-Molecule Imaging of Telomerase RNA Reveals a Recruitment-Retention Model for Telomere Elongation Mol. Cell 2020-05-29 [PMID: 32497497]
• Loe, T K, Li, J S Z Et al. Telomere length heterogeneity in ALT cells is maintained by PML-dependent localization of the BTR complex to telomeres. Genes Dev 2020-05-01 [PMID: 32217664] (IF/IHC, Mouse)
• Lototska L, Yue J X et al. Human RAP1 specifically protects telomeres of senescent cells from DNA damage. EMBO Rep 2020-03-04 [PMID: 32096305] (WB, Human)
• Porreca RM, Herrera-Moyano E, Skourti E et al. TRF1 averts chromatin remodelling, recombination and replication dependent-break induced replication at mouse telomeres Elife 2020-01-14 [PMID: 31934863] (Chemotaxis, Mouse)
• Hua R, Wei H, Liu C et al. FBXO47 regulates telomere-inner nuclear envelope integration by stabilizing TRF2 during meiosis Nucleic Acids Res. 2019-11-14 [PMID: 31724724] (ICC/IF, Mouse)
• Perera ON, Sobinoff AP, Teber ET et al. Telomerase promotes formation of a telomere protective complex in cancer cells Sci Adv 2019-10-01 [PMID: 31616780]
• Li P, Meng Y et al. Nuclear localization of Desmoplakin and its involvement in telomere maintenance. Int J Biol Sci 2019-09-10 [PMID: 31595153] (WB, Human)
• Mukherjee AK, Sharma S, Bagri S et al. Telomere repeat-binding factor 2 binds extensively to extra-telomeric G-quadruplexes and regulates the epigenetic status of several gene promoters J. Biol. Chem. 2019-10-01 [PMID: 31575660] (Chemotaxis, WB, ELISA, Human)
• Masamsetti VP, Low RRJ, Mak KS et al. Replication stress induces mitotic death through parallel pathways regulated by WAPL and telomere deprotection Nat Commun 2019-09-17 [PMID: 31530811] (WB, Human)
• Kroustallaki P, Lirussi L, Carracedo S et al. SMUG1 Promotes Telomere Maintenance through Telomerase RNA Processing Cell Rep 2019-08-13 [PMID: 31412240] (ICC/IF, Mouse)
• Pike AM, Strong MA, Ouyang JPT, Greider CW TIN2 Functions with TPP1/POT1 To Stimulate Telomerase Processivity Mol Cell Biol. 2019-01-01 [PMID: 31383750] (ICC/IF, Human)
• Episkopou H, Diman A, Claude E et al. TSPYL5 Depletion Induces Specific Death of ALT Cells through USP7-Dependent Proteasomal Degradation of POT1 Molecular Cell 2019-07-01 [PMID: 31278054]
• Benyelles M, Episkopou H, O'Donohue MF et al. Impaired telomere integrity and rRNA biogenesis in PARN-deficient patients and knock-out models EMBO Mol Med 2019-06-06 [PMID: 31171653] (WB, Human)
• Lu R, O'Rourke J, Sobinoff A et al. The FANCM-BLM-TOP3A-RMI complex suppresses alternative lengthening of telomeres (ALT) Nat Commun 2019-05-28 [PMID: 31138797] (ICC/IF, Human)
• Jack A Phase Separation as a Model of Nucleoprotein Organization Thesis 2022-01-01 (WB, Human)
  
  Details:
  Dilution used for WB 1:2000
• Zhang S, Lee E, Lee M, Zhang D POLDIP3 facilitates the activation and maintenance of DNA damage checkpoint in response to replication stress Research Square 2022-04-27
• Barry RM Elucidating the Mechanisms by Which Rap1 Protects from Obesity Thesis 2022-01-01
• Chen H, Guo X, Xiao X et al. Condensin II subunit NCAPH2 associates with shelterin protein TRF1 and is required for telomere stability J. Cell. Physiol. 2019-04-26 [PMID: 31026066] (Cytometric Bead Assay Standard, Knockdown, ICC/IF, WB, IP, Human)
• Mendez-Bermudez A, Lototska L et al. Genome-wide Control of Heterochromatin Replication by the Telomere Capping Protein TRF2. Mol Cell 2018-03-05 [PMID: 29727617] (ICC/IF, WB, Human)
• Wang L, Tu Z, et al. Dual roles of TRF1 in tethering telomeres to the nuclear envelope and protecting them from fusion during meiosis. Cell Death Differ 2018-06-01 [PMID: 29311622] (WB, Mouse)
• Hussain T, Saha D et al. Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex. Sci Rep 2017-09-14 [PMID: 28912501] (WB, ELISA, IP, Human)
• Knecht H, Johnson N A et al. Disruption of direct 3D telomere-TRF2 interaction through two molecularly disparate mechanisms is a hallmark of primary Hodgkin and Reed-Sternberg cells. Lab Invest 2017-01-07 [PMID: 28436953] (ICC/IF, Human)
• Stimpson K, Sullivan L, Kuo M et al. Nucleolar organization, ribosomal DNA array stability, and acrocentric chromosome integrity are linked to telomere function Plos One 2014-03-24 [PMID: 24662969] (WB, ICC/IF, Chemotaxis, Human)
• Wang X, Ji C, Zhang H et al. Tousled-like kinase activity is required for transcriptional silencing and suppression of innate immune signaling bioRxiv 2019-04-28 (ICC/IF, Human)
• Lamm N, Masamsetti V, Read M et al. Nuclear filamentous actin functions in the replication stress response J. Agric. Food Chem. 2018-10-15 (Human)
• Storchova R, Palek M, Palkova N et al. Phosphorylation of TRF2 promotes its interaction with TIN2 and regulates DNA damage response at telomeres Nucleic acids research 2023-01-18 [PMID: 36651296] (ICC/IF)
• Dinami R, Pompili L, Petti E et al. MiR-182-3p targets TRF2 and impairs tumor growth of triple-negative breast cancer EMBO molecular medicine 2022-11-25 [PMID: 36426578] (Chemotaxis, Human)
• Wu Y, Li X, Yu J et al. ASF1a inhibition induces p53-dependent growth arrest and senescence of cancer cells. Cell Death Dis 2019-01-28 [PMID: 30692519] (ICC/IF, Human)
• Mukherjee AK, Sharma S, Sengupta S et al. Telomere length-dependent transcription and epigenetic modifications in promoters remote from telomere ends. PLoS Genet. 2018-11-01 [PMID: 30439955] (Chemotaxis, Human)
• Chow TT, Shi X, Wei JH et al. Local enrichment of HP1alpha at telomeres alters their structure and regulation of telomere protection. Nat Commun 2018-09-04 [PMID: 30181605] (Chemotaxis, Human)
• Gu P, Jia S, Takasugi T et al. CTC1-STN1 coordinates G- and C-strand synthesis to regulate telomere length Aging Cell 2018-05-17 [PMID: 29774655] (Human)
• Nelson ND, Dodson LM, Escudero L et al. The C-terminal extension unique to the long isoform of the shelterin component TIN2 enhances its interaction with TRF2 in a phosphorylation- and dyskeratosis congenita-cluster-dependent fashion. Mol. Cell. Biol. 2018-03-26 [PMID: 29581185] (IP)
• Biswas U, Stevense M, Jessberger R. SMC1a Substitutes for Many Meiotic Functions of SMC1b but Cannot Protect Telomeres from Damage Curr. Biol. 2018-01-22 [PMID: 29337080] (ICC/IF)
• Zhang J, Tu Z, Watanabe Y, Shibuya H. Distinct TERB1 Domains Regulate Different Protein Interactions in Meiotic Telomere Movement Cell Rep. 2017-11-14 [PMID: 29141207] (ICC/IF, Mouse)
• Ilic Aleksandar, Lu Sumin, Bhatia Vikram et al. Ubiquitin C-terminal hydrolase isozyme L1 is associated with shelterin complex at interstitial telomeric sites. Epigenetics & Chromatin 2017-01-01 [PMID: 29126443] (IP, Human)
• Li JS, Miralles Fuste J, Simavorian T, Bartocci C. TZAP: A telomere-associated protein involved in telomere length control. Science. 2017-02-10 [PMID: 28082411] (ICC/IF, Human)
• Tu Z, Bayazit MB, Liu H et al. Speedy A-Cdk2 binding mediates initial telomere-nuclear envelope attachment during meiotic prophase I independent of Cdk2 activation. Proc. Natl. Acad. Sci. U.S.A. 2016-12-28 [PMID: 28031483] (WB, Mouse)
• Knecht H, Mai S. The Use of 3D Telomere FISH for the Characterization of the Nuclear Architecture in EBV-Positive Hodgkin's Lymphoma. Methods Mol. Biol. 2016-11-22 [PMID: 27873269]
• Burla R, Carcuro M, Torre ML et al. The telomeric protein AKTIP interacts with A- and B-type lamins and is involved in regulation of cellular senescence. Open Biol. 2016-08-01 [PMID: 27512140] (WB, Human)
• Benhamou Y, Picco V, Raybaud H et al. Telomeric repeat-binding factor 2: a marker for survival and anti-EGFR efficacy in oral carcinoma Oncotarget 2016-07-12 [PMID: 27329590] (WB, Human)
• Cipressa F, Morciano P, Bosso G et al. A role for Separase in telomere protection. Nat Commun. 2016-01-18 [PMID: 26778495] (ICC/IF, Human)
• Manterola M, Sicinski P, Wolgemuth DJ. E-type cyclins modulate telomere integrity in mammalian male meiosis. Chromosoma. 2015-12-28 [PMID: 26712234] (ICC/IF, Mouse)
• Sishc BJ, Nelson CB, McKenna MJ et al. Telomeres and Telomerase in the Radiation Response: Implications for Instability, Reprograming, and Carcinogenesis. Front Oncol 2015-11-24 [PMID: 26636039]
• Shibuya H, Hernandez-Hernandez A, Morimoto A et al. MAJIN Links Telomeric DNA to the Nuclear Membrane by Exchanging Telomere Cap. Cell. 2015-11-19 [PMID: 26548954] (ICC/IF, Mouse)
  
  Details:
  TRF-2 antibody used for ICC/IF on wild-type pachytene-spermatocytes (Fig 5).
• Ci X, Li B, Ma X et al. Bortezomib-mediated down-regulation of telomerase and disruption of telomere homeostasis contributes to apoptosis of malignant cells. Oncotarget 2015-11-10 [PMID: 26472030] (WB)
• Badie S, Carlos AR, Folio C et al. BRCA1 and CtIP promote alternative non-homologous end-joining at uncapped telomeres. EMBO J 2015-02-03 [PMID: 25582120] (WB, Mouse)
• Robin JD, Ludlow AT, Batten K et al. Telomere position effect: regulation of gene expression with progressive telomere shortening over long distances. Genes Dev 2014-11-15 [PMID: 25403178] (Human)
• Wood Am, Rendtlew Danielsen Jm, Lucas Ca et al. TRF2 and lamin A/C interact to facilitate the functional organization of chromosome ends. Nat Commun. 2014-11-17 [PMID: 25399868] (Chemotaxis, Human)
• Arora R, Lee Y, Wischnewski H et al. RnaseH1 regulates TERRA-telomeric DnA hybrids and telomere maintenance in ALT tumour cells. Nat Commun. 2014-10-21 [PMID: 25330849] (ICC/IF, Chemotaxis, WB, Human)
• Wojdyla L, Stone AL, Sethakorn N et al. T-oligo as an anticancer agent in colorectal cancer. Biochem. Biophys. Res. Commun. 2014-03-12 [PMID: 24632202] (ICC/IF, Human)
• Islam MA, Thomas SD, Murty VV, Miller DM. c-Myc quadruplex-forming sequence Pu-27 induces extensive damage in both telomeric and non-telomeric regions of DNA. J. Biol. Chem. 2014-01-27 [PMID: 24464582] (WB, Human)
• Shibuya H, Ishiguro K, Watanabe Y. The TRF1-binding protein TERB1 promotes chromosome movement and telomere rigidity in meiosis. Nat. Cell Biol. 2014-02-01 [PMID: 24413433] (ICC/IF, Mouse)
• Pitman RT, Wojdyla L, Puri N. Mechanism of DNA damage responses induced by exposure to an oligonucleotide homologous to the telomere overhang in melanoma. Oncotarget. 2013-05-01 [PMID: 23800953] (WB, Human)
• Kappei D, Butter F, Benda C et al. HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment. EMBO J 2013-06-12 [PMID: 23685356] (IP, Chemotaxis, Human)
• Chang FT, McGhie JD, Chan FL et al. PML bodies provide an important platform for the maintenance of telomeric chromatin integrity in embryonic stem cells. Nucleic Acids Res 2013-03-11 [PMID: 23444137] (ICC/IF, Mouse)
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Reviews for TRF-2 Antibody (NB110-57130) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Robert Lu ICC Human 10/11/2020

Summary

• Application: Immunocytochemistry
• Sample Tested: U-2 OS cells,U2OS cell line
• Species: Human
• Lot: D-1

Comments

• Comments: Antibody used at 1:400 dilution in antibody dilution buffer and incubated for 2 hours at room-temperature. Detected with Donkey anti-rabbit AF488 antibody.

reviewed by: David Van Ly ChIP Mouse 12/01/2014

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: Mouse
• Species: Mouse
• Lot: C-1

FAQs for TRF-2 Antibody (NB110-57130). (Showing 1 - 3 of 3 FAQs).

1. Are the TRF-2 antibodies validated in Simple Western?
   • Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.
2. Does TRF-2 antibodies comes in lyophilized form?
   • we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.
3. What the theoretical molecular weight for TRF-2 antibodies?
   • The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.

Bioinformatics

• Gene Symbol: TERF2