TRF-2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB110-57130] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Simple Western: TRF-2 Antibody [NB110-57130] - Lane view shows a specific band for TRF2 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: TRF-2 Antibody [NB110-57130] - Analysis of HeLa whole cell lysate (A), HeLa nuclear cell lysate (B), k562 cell lysate (C), HepG2 cell lysate (D), NIH/3T3 cell lysate (E), CHO cell lysate (F), PC12 cell ...read more
Immunohistochemistry-Paraffin: TRF-2 Antibody [NB110-57130] - Analysis in xenografted human breast cancer tissue using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: TRF-2 Antibody [NB110-57130] - Analysis of FFPE human breast cancer tissue with rabbit polyclonal TRF2 antibody at a dilution of 1:200. The staining was developed with HRP-DAB detection ...read more
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB110-57130] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Flow Cytometry: TRF-2 Antibody [NB110-57130] - An intracellular stain was performed on HeLa cells with TRF-2 Antibody NB110-57130 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA ...read more
Chromatin Immunoprecipitation: TRF-2 Antibody [NB110-57130] - Analysis in mouse. Titrated TRF2 antibody to determine concentration required for ChIP experiment. ChIP image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB110-57130] - RNAi-mediated depletion of human separase (ESPL1) induces TIFs. Control scrambled siRNA- (control) and ESPL1 siRNA-treated fibroblasts stained with ...read more
Immunohistochemistry-Paraffin: TRF-2 Antibody [NB110-57130] - Analysis of FFPE human breast cancer tissue with rabbit polyclonal TRF2 antibody at 1:200 dilution. The staining was developed with HRP-DAB detection method ...read more
Flow Cytometry: TRF-2 Antibody [NB110-57130] - An intracellular stain was performed on HeLa cells with TRF-2 Antibody NB110-57130AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, PmSpecies Glossary
Applications WB, Simple Western, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

TRF-2 Antibody Summary

Immunogen
This TRF-2 Antibody was developed against Baculovirus purified TRF2 protein.
Localization
Nuclear
Marker
Telomeres marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TERF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:5000
  • Simple Western 1:25
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Dot Blot
  • ELISA
  • Flow Cytometry 2 - 5 ug / 10^5 cells
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • Knockdown Validated
Application Notes
This TRF2 antibody is useful for ChIP, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band at approx. 56 kDa is seen. Immunoprecipitation was reported in scientific literature. Use in Knockdown Validated, and Dot blot reported in scientific literature (PMID: 31026066). Use in ELISA reported in scientific literature (PMID: 31575660). In ICC/IF, nuclear staining was observed in HeLa cells. In IHC, nuclear staining was observed in xenografted human breast cancer tisSue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
59.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
TRF-2 Overexpression Lysate
HeLa Nuclear Cell Lysate
HeLa Whole Cell Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NB110-57130 in the following applications:

Publications
Read Publications using
NB110-57130 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRF-2 Antibody

  • Telomeric DNA-binding protein
  • telomeric repeat binding factor 2
  • Telomeric repeat binding protein 2
  • telomeric repeat-binding factor 2
  • TERF2
  • TRBF2
  • TRF2
  • TRF-2
  • TTAGGG repeat binding factor 2
  • TTAGGG repeat-binding factor 2

Background

Originally discovered as part of shelterin complex, telomeric repeat-binding factor 2 (TRF2, also called TERF2 or TRB2) is a ubiquitously expressed nuclear protein (55-60 kDa) involved in telomere homeostasis. TRF2 contains an N-terminal GAR domain, a central TRFH dimerization domain, and a C-terminal SAND/MYB-type DNA binding domain. Trf2 RNA has 10 exons and alternative splicing in rodents produces a truncated form, TRF2-S, which lacks the DNA binding domain and nuclear localization signal (NLS) (1).

Both TRF2 and TRF1 bind to telomeric double stranded 5'-TTAGGG-3' DNA repeats, then recruit RAP1, TIN2, TPP1, and POT1 for the assembly of the shelterin complex. The telomeric association of TRF2 is greatly increased in the S phase of the cell cycle (2). Loss of TRF2 leads to telomere shortening, the DNA damage response, chromosomal instability, and replicative senescence. Interestingly, the contribution of TRF2 to telomere shortening via a telomerase-independent mechanism has also been reported (3). In conjunction with the exonuclease, Apollo, TRF2 protects telomeres during replication and negatively regulates the accumulation of DNA topoisomerase (TOP1, TOP2A and TOP2B).

TRF2 has been implicated in cancer, shown to be a major oncogene in telomerase-deficient mice. A link to Werner syndrome, a premature aging disease caused by the loss of WRN, has been reported based on TRF2 recruitment of WRN for processing of telomeric DNA (4). TRF2 expression is increased during human embryonic stem cell differentiation and has been shown to interact with Repressor Element-1 Silencing Transcription Factor (REST), protecting it from proteasomal degradation (5).

References

1. Grammatikakis, I., Zhang, P., Mattson, M. P., & Gorospe, M. (2016). The long and the short of TRF2 in neurogenesis. Cell cycle (Georgetown, Tex.), 15(22), 3026-3032. PMID: 27565210

2. Li, F., Kim, H., Ji, Z., Zhang, T., Chen, B., Ge, Y., Hu, Y., Feng, X., Han, X., Xu, H., Zhang, Y., Yu, H., Liu, D., Ma, W., & Songyang, Z. (2018). The BUB3-BUB1 Complex Promotes Telomere DNA Replication. Molecular cell, 70(3), 395-407. PMID: 29727616

3. Ancelin, K., Brunori, M., Bauwens, S., Koering, C. E., Brun, C., Ricoul, M., Pommier, J. P., Sabatier, L., & Gilson, E. (2002). Targeting assay to study the cis functions of human telomeric proteins: evidence for inhibition of telomerase by TRF1 and for activation of telomere degradation by TRF2. Molecular and cellular biology, 22(10), 3474-3487. PMID: 11971978

4. Machwe A, Xiao L, & Orren DK. (2004) TRF2 recruits the Werner syndrome (WRN) exonuclease for processing of telomeric DNA. Oncogene. 23(1):149-56. PMID: 14712220.

5. Diotti, R., & Loayza, D. (2011). Shelterin complex and associated factors at human telomeres. Nucleus (Austin, Tex.), 2(2), 119-135. PMID: 21738835

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-57130
Species: Hu, Mu, Rt, ChHa, Pm
Applications: WB, Simple Western, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, KD

Publications for TRF-2 Antibody (NB110-57130)(90)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: ChIP, DB, ELISA, ICC/IF, IHC, IP, Knockdown, WB.


Filter By Application
ChIP
(9)
DB
(1)
ELISA
(2)
ICC/IF
(31)
IHC
(2)
IP
(8)
Knockdown
(1)
WB
(29)
All Applications
Filter By Species
Human
(47)
Mouse
(18)
All Species
Showing Publications 1 - 10 of 90. Show All 90 Publications.
Publications using NB110-57130 Applications Species
Kim C, Sung S, Kim JS et al. Telomeres reforged with non-telomeric sequences in mouse embryonic stem cells Nature communications Feb 17 2021 [PMID: 33597549] (ICC/IF, Mouse) ICC/IF Mouse
Lamm N, Read MN, Nobis M et al. Nuclear F-actin counteracts nuclear deformation and promotes fork repair during replication stress Nature cell biology Dec 1 2020 [PMID: 33257806]
Lamm N, Masamsetti V, Read M et al. Nuclear filamentous actin functions in the replication stress response J. Agric. Food Chem. Oct 15 2018 (Human) Human
Wang X, Ji C, Zhang H et al. Tousled-like kinase activity is required for transcriptional silencing and suppression of innate immune signaling bioRxiv Apr 28 2019 (ICC/IF, Human) ICC/IF Human
Kosebent EG, Ozturk S The spatiotemporal expression of TERT and telomere repeat binding proteins in the postnatal mouse testes Andrologia Feb 5 2021 [PMID: 33544428]
Zhang JM, Genois MM, Ouyang J, et al. Alternative lengthening of telomeres is a self-perpetuating process in ALT-associated PML bodies Molecular cell Jan 8 2021 [PMID: 33453166]
Viceconte N, Loriot A, Lona Abreu P et al. PAR-TERRA is the main contributor to telomeric repeat-containing RNA transcripts in normal and cancer mouse cells RNA Oct 30 2020 [PMID: 33127860] (WB, Mouse) WB Mouse
Pinzaru AM, Kareh M, Lamm N et al. Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore Genes Dev Oct 29 2020 [PMID: 33122293] (WB, Human) WB Human
Querido E, Sfeir A, Chartrand P Imaging of Telomerase RNA by Single-Molecule Inexpensive FISH Combined with Immunofluorescence STAR Protoc Sep 18 2020 [PMID: 33111129] (ICC/IF, Human) ICC/IF Human
Hoang SM, Kaminski N, Bhargava R et al. Regulation of ALT-associated homology-directed repair by polyADP-ribosylation Nat Struct Mol Biol Oct 12 2020 [PMID: 33046907]
Show All 90 Publications.

Reviews for TRF-2 Antibody (NB110-57130) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB110-57130:
Filter by Applications
ICC
(1)
ChIP
(1)
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Human
(1)
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry TRF-2 NB110-57130
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5
reviewed by:
Anonymous
ICC Human 10/11/2020
View

Summary

ApplicationImmunocytochemistry
Sample TestedU2OS cell line,U-2 OS cells
SpeciesHuman
LotD-1

Comments

CommentsAntibody used at 1:400 dilution in antibody dilution buffer and incubated for 2 hours at room-temperature. Detected with Donkey anti-rabbit AF488 antibody.
Chromatin Immunoprecipitation TRF-2 NB110-57130
Enlarge
5
reviewed by:
Anonymous
ChIP Mouse 12/01/2014
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedMouse
SpeciesMouse
LotC-1
Special ApplicationsTitrated TRF2 antibody to determine concentration required for ChIP experiment. It worked perfectly generating the graph shown.
FileView PDF

Details

Detection NotesChIP experiment

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for TRF-2 Antibody (NB110-57130). (Showing 1 - 3 of 3 FAQs).

  1. Are the TRF-2 antibodies validated in Simple Western?
    • Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.
  2. Does TRF-2 antibodies comes in lyophilized form?
    • we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.
  3. What the theoretical molecular weight for TRF-2 antibodies?
    • The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-57130AF350
Alexa Fluor 405 NB110-57130AF405
Alexa Fluor 488 NB110-57130AF488
Alexa Fluor 532 NB110-57130AF532
Alexa Fluor 594 NB110-57130AF594
Alexa Fluor 647 NB110-57130AF647
Alexa Fluor 700 NB110-57130AF700
Alexa Fluor 750 NB110-57130AF750
Biotin NB110-57130B
DyLight 350 NB110-57130UV
DyLight 405 NB110-57130V
DyLight 488 NB110-57130G
DyLight 550 NB110-57130R
DyLight 594 NB110-57130DL594
DyLight 650 NB110-57130C
DyLight 680 NB110-57130FR
DyLight 755 NB110-57130IR
FITC NB110-57130F
HRP NB110-57130H
Janelia Fluor 549 NB110-57130JF549
Janelia Fluor 646 NB110-57130JF646

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Blogs on TRF-2.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

TRF2: Telomere Maintenance and Protection
TRF2 (telomeric repeat-binding factor 2) is a homodimeric protein that binds the double-stranded 5'-TTAGGG-3' repeat in telomeres. It plays a critical role in the maintenance of telomeres, protecting telomeres against end-to-end chromosomal fusion, an...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
10/11/2020
Application: ICC
Species: Human

Anonymous
12/01/2014
Application: ChIP
Species: Mouse

Bioinformatics

Gene Symbol TERF2