Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB110-57130] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Simple Western: TRF-2 Antibody [NB110-57130] - Lane view shows a specific band for TRF2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: TRF-2 Antibody [NB110-57130] - Analysis of HeLa whole cell lysate (A), HeLa nuclear cell lysate (B), k562 cell lysate (C), HepG2 cell lysate (D), NIH/3T3 cell lysate (E), CHO cell lysate (F), PC12 cell ...read more
Immunohistochemistry-Paraffin: TRF-2 Antibody [NB110-57130] - Analysis in xenografted human breast cancer tissue using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: TRF-2 Antibody [NB110-57130] - Analysis of FFPE human breast cancer tissue with rabbit polyclonal TRF2 antibody at a dilution of 1:200. The staining was developed with HRP-DAB detection ...read more
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB110-57130] - RNAi-mediated depletion of human separase (ESPL1) induces TIFs. Control scrambled siRNA- (control) and ESPL1 siRNA-treated fibroblasts stained with ...read more
Flow Cytometry: TRF-2 Antibody [NB110-57130] - An intracellular stain was performed on HeLa cells with TRF-2 Antibody NB110-57130AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Chromatin Immunoprecipitation: TRF-2 Antibody [NB110-57130] - Analysis in mouse. Titrated TRF2 antibody to determine concentration required for ChIP experiment. Image from verified customer review.
Immunohistochemistry-Paraffin: TRF-2 Antibody [NB110-57130] - Analysis of FFPE human breast cancer tissue with rabbit polyclonal TRF2 antibody at 1:200 dilution. The staining was developed with HRP-DAB detection method ...read more
This TRF2 antibody is useful for ChIP, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band at approx. 56 kDa is seen. Immunoprecipitation was reported in scientific literature. Use in Knockdown Validated, and Dot blot reported in scientific literature (PMID: 31026066). Use in ELISA reported in scientific literature (PMID:31575660). In ICC/IF, nuclear staining was observed in HeLa cells. In IHC, nuclear staining was observed in xenografted human breast cancer tisSue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
56 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for TRF-2 Antibody
Telomeric DNA-binding protein
telomeric repeat binding factor 2
Telomeric repeat binding protein 2
telomeric repeat-binding factor 2
TTAGGG repeat binding factor 2
TTAGGG repeat-binding factor 2
TRF2 (telomeric repeat-binding factor 2) is a homodimeric protein that binds the telomeric double-stranded 5'-TTAGGG-3' repeat for playing a critical role in the maintenance of telomeres as well as telomere's protection against end-to-end chromosomal fusion, and recruit a number of factors/enzymes required for telomere protection including shelterin complex/telosome (TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1), TERF2IP/RAP1 and DCLRE1B/Apollo. Together with DCLRE1B/Apollo, TRF2 plays a key role in T-loopformation by generating 3' single-stranded overhang at the leading end telomeres and is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage for preventing aberrant telomere topology. TRF2 recruits TERF2IP/RAP1 to telomeres, thereby participating into repressing homology-directed repair, which can impact telomeres length. Damaged telomeres have been shown to become associated with DNA-damage response factors such as 53BP1, MDC1, gamma-H2AX, ATM and the Mre11/Nbs1/Rad50 or MRN complex, and activate the ATM signaling, leading to cell cycle arrest mediated by the p53/p21 pathway. TRF2 interacts with PNUTS, MCPH1 and DEAD-box RNA helicase DDX39 also for regulating telomere length, telomeric DNA-damage response as well as genome integrity.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
TRF2: Telomere Maintenance and Protection TRF2 (telomeric repeat-binding factor 2) is a homodimeric protein that binds the double-stranded 5'-TTAGGG-3' repeat in telomeres. It plays a critical role in the maintenance of telomeres, protecting telomeres against end-to-end chromosomal fusion, an... Read full blog post.
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