TRF-2 Antibody (NB110-57130)

Images

 
Western Blot: TRF-2 Antibody [NB110-57130] - Analysis of HeLa whole cell lysate (A), HeLa nuclear cell lysate (B), k562 cell lysate (C), HepG2 cell lysate (D), NIH/3T3 cell lysate (E), CHO cell lysate (F), PC12 cell ...read more
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB110-57130] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: TRF-2 Antibody [NB110-57130] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast cancer tissue with rabbit polyclonal TRF2 antibody at a dilution of 1:200. The ...read more
Chromatin Immunoprecipitation: TRF-2 Antibody [NB110-57130] - Analysis in mouse. Titrated TRF2 antibody to determine concentration required for ChIP experiment. Image from verified customer review.
Immunohistochemistry-Paraffin: TRF-2 Antibody [NB110-57130] - Analysis in xenografted human breast cancer tissue using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: TRF-2 Antibody [NB110-57130] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast cancer tissue with rabbit polyclonal TRF2 antibody at 1:200 dilution. The staining was ...read more
Simple Western: TRF-2 Antibody [NB110-57130] - Simple Western lane view shows a specific band for TRF2 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, PmSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

TRF-2 Antibody Summary

Immunogen
Baculovirus purified TRF2 protein.
Localization
Nuclear
Marker
Telomeres marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TERF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:5000
  • Simple Western 1:25
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Immunoprecipitation
Application Notes
This TRF2 antibody is useful for ChIP, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band at approx. 56 kDa is seen. Immunoprecipitation was reported in scientific literature. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC, nuclear staining was observed in xenografted human breast cancer tisSue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
TRF-2 Lysate (NBL1-16815)
HeLa Lysate (NB800-PC9)
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NB110-57130 in the following applications:

Publications
Read Publications using
NB110-57130 in the following applications:

  • 5 publications
  • 1 publication
  • 13 publications
  • IHC
    1 publication
  • IP
    3 publications
  • WB
    19 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRF-2 Antibody

  • Telomeric DNA-binding protein
  • telomeric repeat binding factor 2
  • Telomeric repeat binding protein 2
  • telomeric repeat-binding factor 2
  • TERF 2
  • TERF2
  • TRBF 2
  • TRBF2
  • TRF 2
  • TRF2
  • TRF-2
  • TTAGGG repeat binding factor 2
  • TTAGGG repeat-binding factor 2

Background

TRF2 (telomeric repeat-binding factor 2) is a homodimeric protein that binds the telomeric double-stranded 5'-TTAGGG-3' repeat for playing a critical role in the maintenance of telomeres as well as telomere's protection against end-to-end chromosomal fusion, and recruit a number of factors/enzymes required for telomere protection including shelterin complex/telosome (TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1), TERF2IP/RAP1 and DCLRE1B/Apollo. Together with DCLRE1B/Apollo, TRF2 plays a key role in T-loopformation by generating 3' single-stranded overhang at the leading end telomeres and is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage for preventing aberrant telomere topology. TRF2 recruits TERF2IP/RAP1 to telomeres, thereby participating into repressing homology-directed repair, which can impact telomeres length. Damaged telomeres have been shown to become associated with DNA-damage response factors such as 53BP1, MDC1, gamma-H2AX, ATM and the Mre11/Nbs1/Rad50 or MRN complex, and activate the ATM signaling, leading to cell cycle arrest mediated by the p53/p21 pathway. TRF2 interacts with PNUTS, MCPH1 and DEAD-box RNA helicase DDX39 also for regulating telomere length, telomeric DNA-damage response as well as genome integrity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-57130
Species: Hu, Mu, Rt, ChHa, Pm
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP

Publications for TRF-2 Antibody (NB110-57130)(46)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ChIP, ICC-IF, ICC/IF, IHC, IP, WB.


Filter By Application
ChIP
(5)
ICC-IF
(1)
ICC/IF
(13)
IHC
(1)
IP
(3)
WB
(19)
All Applications
Filter By Species
Human
(22)
Mouse
(12)
All Species
Showing Publications 1 - 10 of 46. Show All 46 Publications.
Publications using NB110-57130 Applications Species
Tu Z, Bayazit MB, Liu H et al. Speedy A-Cdk2 binding mediates initial telomere-nuclear envelope attachment during meiotic prophase I independent of Cdk2 activation. Proc. Natl. Acad. Sci. U.S.A. Dec 28 2016 [PMID: 28031483] (WB, Mouse) WB Mouse
Knecht H, Mai S. The Use of 3D Telomere FISH for the Characterization of the Nuclear Architecture in EBV-Positive Hodgkin's Lymphoma. Methods Mol. Biol. Nov 22 2016 [PMID: 27873269]
Burla R, Carcuro M, Torre ML et al. The telomeric protein AKTIP interacts with A- and B-type lamins and is involved in regulation of cellular senescence. Open Biol. Aug 1 2016 [PMID: 27512140] (WB, Human) WB Human
Benhamou Y, Picco V, Raybaud H et al. Telomeric repeat-binding factor 2: a marker for survival and anti-EGFR efficacy in oral carcinoma Oncotarget Jul 12 2016 [PMID: 27329590] (WB, Human) WB Human
Cipressa F, Morciano P, Bosso G et al. A role for Separase in telomere protection. Nat Commun. 2016 Jan 18 [PMID: 26778495] (ICC/IF, Human) ICC/IF Human
Manterola M, Sicinski P, Wolgemuth DJ. E-type cyclins modulate telomere integrity in mammalian male meiosis. Chromosoma. 2015 Dec 28 [PMID: 26712234] (ICC/IF, Mouse) ICC/IF Mouse
Sishc BJ, Nelson CB, McKenna MJ et al. Telomeres and Telomerase in the Radiation Response: Implications for Instability, Reprograming, and Carcinogenesis. Front Oncol 2015 Nov 24 [PMID: 26636039]
Shibuya H, Hernandez-Hernandez A, Morimoto A et al. MAJIN Links Telomeric DNA to the Nuclear Membrane by Exchanging Telomere Cap. Cell. 2015 Nov 19 [PMID: 26548954] (ICC/IF, Mouse)

Details:
TRF-2 antibody used for ICC/IF on wild-type pachytene-spermatocytes (Fig 5).
ICC/IF Mouse
Ci X, Li B, Ma X et al. Bortezomib-mediated down-regulation of telomerase and disruption of telomere homeostasis contributes to apoptosis of malignant cells. Oncotarget 2015 Nov 10 [PMID: 26472030] (WB) WB
Badie S, Carlos AR, Folio C et al. BRCA1 and CtIP promote alternative non-homologous end-joining at uncapped telomeres EMBO J. 2015 Jan 12 [PMID: 25582120] (WB, Mouse) WB Mouse
Show All 46 Publications.

Review for TRF-2 Antibody (NB110-57130) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: ChIP.

Reviews using NB110-57130:
Filter by Applications
ChIP
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Chromatin Immunoprecipitation TRF-2 NB110-57130
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reviewed by:
Anonymous
ChIP Mouse 12/01/2014
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedMouse
SpeciesMouse
LotC-1
Special ApplicationsTitrated TRF2 antibody to determine concentration required for ChIP experiment. It worked perfectly generating the graph shown.
FileView PDF

Details

Detection NotesChIP experiment

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for TRF-2 Antibody (NB110-57130). (Showing 1 - 3 of 3 FAQs).

  1. Are the TRF-2 antibodies validated in Simple Western?
    • Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.
  2. Does TRF-2 antibodies comes in lyophilized form?
    • we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.
  3. What the theoretical molecular weight for TRF-2 antibodies?
    • The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-57130AF405
Alexa Fluor 488 Labeled NB110-57130AF488
Alexa Fluor 647 Labeled NB110-57130AF647
Alexa Fluor 700 Labeled NB110-57130AF700
Biotin Labeled NB110-57130B
DyLight 488 Labeled NB110-57130G
DyLight 550 Labeled NB110-57130R
DyLight 650 Labeled NB110-57130C
HRP Labeled NB110-57130H

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Blogs on TRF-2.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

TRF2: Telomere Maintenance and Protection
TRF2 (telomeric repeat-binding factor 2) is a homodimeric protein that binds the double-stranded 5'-TTAGGG-3' repeat in telomeres. It plays a critical role in the maintenance of telomeres, protecting telomeres against end-to-end chromosomal fusion, an...  Read full blog post.

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Recent Reviews

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1
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Anonymous
12/01/2014
Application: ChIP
Species: Mouse

Bioinformatics

Gene Symbol TERF2
Entrez

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