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TRF-2 Antibody - BSA Free

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Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB110-57130] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Simple Western: TRF-2 Antibody [NB110-57130] - Lane view shows a specific band for TRF2 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: TRF-2 Antibody [NB110-57130] - Analysis of HeLa whole cell lysate (A), HeLa nuclear cell lysate (B), k562 cell lysate (C), HepG2 cell lysate (D), NIH/3T3 cell lysate (E), CHO cell lysate (F), PC12 cell ...read more
Immunohistochemistry-Paraffin: TRF-2 Antibody [NB110-57130] - Analysis in xenografted human breast cancer tissue using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: TRF-2 Antibody [NB110-57130] - Analysis of FFPE human breast cancer tissue with rabbit polyclonal TRF2 antibody at a dilution of 1:200. The staining was developed with HRP-DAB detection ...read more
Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB110-57130] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Flow Cytometry: TRF-2 Antibody [NB110-57130] - An intracellular stain was performed on HeLa cells with TRF-2 Antibody NB110-57130 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA ...read more
Chromatin Immunoprecipitation: TRF-2 Antibody [NB110-57130] - Analysis in mouse. Titrated TRF2 antibody to determine concentration required for ChIP experiment. ChIP image submitted by a verified customer review.
Genetic Strategies: Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB110-57130] - RNAi-mediated depletion of human separase (ESPL1) induces TIFs. Control scrambled siRNA- (control) and ESPL1 ...read more
Immunohistochemistry-Paraffin: TRF-2 Antibody [NB110-57130] - Analysis of FFPE human breast cancer tissue with rabbit polyclonal TRF2 antibody at 1:200 dilution. The staining was developed with HRP-DAB detection method ...read more
Flow Cytometry: TRF-2 Antibody [NB110-57130] - An intracellular stain was performed on HeLa cells with TRF-2 Antibody NB110-57130AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Telomere deprotection contributes to replication stress lethality. A) Western blots of whole cell extracts from HT1080 6TG cells stably transduced with control, TRF2 shRNA (TRF sh-F) or TRF2 over expression (TRF2OE) ...read more
RNAi-mediated depletion of human separase (ESPL1) induces TIFs.(a) Control scrambled siRNA- (control) and ESPL1 siRNA-treated fibroblasts stained with anti-p53-binding protein 1 (53BP1; green) and anti-TRF2 (red). It is ...read more
Polyphyllin I triggers PINK1/PARK2-dependent mitophagyA. MDA-MB-231 cells were treated with 8 μM PPI for different periods of time as indicated, and PARK2, P62, and ubiquitin (UB) levels in mitochondrial fractions were ...read more
PHD3 depletion stabilizes hypoxic p27 expression by increasing p27 half-life. a Cell cycle arrest at G0 and subsequent release shows an increase of p27 expression in siPHD3 exposed cells. b Quantification for p27 ...read more
Immunohistochemical staining of HIF-1 alpha , VEGF-A and VEGF-C in normal renal tissue (A-C) and clear cell renal cell carcinoma (CCRCC) (D-F). A homogeneous cytoplasmic staining of tubular cells and weak staining in ...read more
Resistance of FANCA-mutant cells with defective FANCD2 function to hydrogen peroxide (H2O2). (a) Representative images illustrating staining for subnuclear FANCD2 foci in isogenic fibroblast pairs, either deficient for ...read more
gamma -Taxilin ablation causes ER stress responses and apoptosis in normoxic cells. (a) Knockdown of gamma -taxilin expression by small interfering RNA (siRNA) in HeLa S3 cells. Two different siRNA constructs are ...read more
Damage response is restricted to MG. (A) RT-PCR analysis for Slc1a3, the gene encoding GLAST, at the indicated times after injury. (B) RT-PCR for MG and a photoreceptor-specific marker (Nrl) in GLAST-positive and ...read more
Effect of ET-1 and Cx43 on HIF-1 alpha expression and glucose uptake when c-Src is inhibited.Astrocytes were preincubated with 100 ng/µL PP2 (c-Src inhibitor) or 100 ng/µL PP3 (inactive analogue) for 1 h. Then, cells ...read more
Genotyping of transgenes.PCR genotyping was performed for all four transgenes as described in materials and methods. Sizes of the amplified products obtained are: 240 bp for Hif-1 alpha (wild type); 274 bp for Hif-1 ...read more
The expression patterns of Yap and GFAP-Cre recombinase in postnatal mouse eyes. (A) Schematic of a transverse section of mouse eye. (B-C) Immunostaining with anti-Yap antibody (green) on frozen eye sections at ...read more
Representative picture of western blot in histopathologically unchanged tissue (N) and primary cancerous tissue (C) from patients with CRC. Immunodetection of bands was performed with Rp anti- PHD1, - PHD2, - PHD3 and - ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, PmSpecies Glossary
Applications WB, Simple Western, DB, ELISA, Flow, ICC/IF, IHC, IP, ChIP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

TRF-2 Antibody - BSA Free Summary

Immunogen
This TRF-2 Antibody was developed against Baculovirus purified TRF2 protein.
Localization
Nuclear
Marker
Telomeres marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TERF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Dot Blot reported in scientific literature (PMID 31026066)
  • ELISA reported in scientific literature (PMID 31575660)
  • Flow Cytometry 1-5 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature
  • Knockdown Validated reported in scientific literature (PMID 31026066)
  • Simple Western 1:25
  • Western Blot 1:2000 - 1:5000
Application Notes
In Western blot, a band at approx. 56 kDa is seen. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC, nuclear staining was observed in xenografted human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
59.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Nuclear Cell Lysate
HeLa Whole Cell Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NB110-57130 in the following applications:

Publications
Read Publications using
NB110-57130 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRF-2 Antibody - BSA Free

  • Telomeric DNA-binding protein
  • telomeric repeat binding factor 2
  • Telomeric repeat binding protein 2
  • telomeric repeat-binding factor 2
  • TERF2
  • TRBF2
  • TRF2
  • TRF-2
  • TTAGGG repeat binding factor 2
  • TTAGGG repeat-binding factor 2

Background

Originally discovered as part of shelterin complex, telomeric repeat-binding factor 2 (TRF2, also called TERF2 or TRB2) is a ubiquitously expressed nuclear protein (55-60 kDa) involved in telomere homeostasis. TRF2 contains an N-terminal GAR domain, a central TRFH dimerization domain, and a C-terminal SAND/MYB-type DNA binding domain. Trf2 RNA has 10 exons and alternative splicing in rodents produces a truncated form, TRF2-S, which lacks the DNA binding domain and nuclear localization signal (NLS) (1).

Both TRF2 and TRF1 bind to telomeric double stranded 5'-TTAGGG-3' DNA repeats, then recruit RAP1, TIN2, TPP1, and POT1 for the assembly of the shelterin complex. The telomeric association of TRF2 is greatly increased in the S phase of the cell cycle (2). Loss of TRF2 leads to telomere shortening, the DNA damage response, chromosomal instability, and replicative senescence. Interestingly, the contribution of TRF2 to telomere shortening via a telomerase-independent mechanism has also been reported (3). In conjunction with the exonuclease, Apollo, TRF2 protects telomeres during replication and negatively regulates the accumulation of DNA topoisomerase (TOP1, TOP2A and TOP2B).

TRF2 has been implicated in cancer, shown to be a major oncogene in telomerase-deficient mice. A link to Werner syndrome, a premature aging disease caused by the loss of WRN, has been reported based on TRF2 recruitment of WRN for processing of telomeric DNA (4). TRF2 expression is increased during human embryonic stem cell differentiation and has been shown to interact with Repressor Element-1 Silencing Transcription Factor (REST), protecting it from proteasomal degradation (5).

References

1. Grammatikakis, I., Zhang, P., Mattson, M. P., & Gorospe, M. (2016). The long and the short of TRF2 in neurogenesis. Cell cycle (Georgetown, Tex.), 15(22), 3026-3032. PMID: 27565210

2. Li, F., Kim, H., Ji, Z., Zhang, T., Chen, B., Ge, Y., Hu, Y., Feng, X., Han, X., Xu, H., Zhang, Y., Yu, H., Liu, D., Ma, W., & Songyang, Z. (2018). The BUB3-BUB1 Complex Promotes Telomere DNA Replication. Molecular cell, 70(3), 395-407. PMID: 29727616

3. Ancelin, K., Brunori, M., Bauwens, S., Koering, C. E., Brun, C., Ricoul, M., Pommier, J. P., Sabatier, L., & Gilson, E. (2002). Targeting assay to study the cis functions of human telomeric proteins: evidence for inhibition of telomerase by TRF1 and for activation of telomere degradation by TRF2. Molecular and cellular biology, 22(10), 3474-3487. PMID: 11971978

4. Machwe A, Xiao L, & Orren DK. (2004) TRF2 recruits the Werner syndrome (WRN) exonuclease for processing of telomeric DNA. Oncogene. 23(1):149-56. PMID: 14712220.

5. Diotti, R., & Loayza, D. (2011). Shelterin complex and associated factors at human telomeres. Nucleus (Austin, Tex.), 2(2), 119-135. PMID: 21738835

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-57130
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Publications for TRF-2 Antibody (NB110-57130)(136)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 12 applications: B/N, ChIP, Chemotaxis, Cytometric Bead Assay Standard, ELISA, FISH, ICC/IF, IF/IHC, IP, Knockdown, PLA, WB.


Filter By Application
B/N
(4)
ChIP
(2)
Chemotaxis
(11)
Cytometric Bead Assay Standard
(1)
ELISA
(2)
FISH
(1)
ICC/IF
(40)
IF/IHC
(2)
IP
(8)
Knockdown
(1)
PLA
(1)
WB
(46)
All Applications
Filter By Species
Human
(64)
Mouse
(24)
Rat
(1)
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Showing Publications 1 - 10 of 136. Show All 136 Publications.
Publications using NB110-57130 Applications Species
Amin A, Morello M, Petrara M et al. Short-Term TERT Inhibition Impairs Cellular Proliferation via a Telomere Length-Independent Mechanism and Can Be Exploited as a Potential Anticancer Approach Cancers 2023-05-09 [PMID: 37345011] (WB, Human) WB Human
Wang X, Ji C, Zhang H et al. Tousled-like kinase activity is required for transcriptional silencing and suppression of innate immune signaling bioRxiv 2019-04-28 (ICC/IF, Human) ICC/IF Human
Meng X, Yao D, Imaizumi K et al. Assembloid CRISPR screens reveal impact of disease genes in human neurodevelopment Nature 2023-10-01 [PMID: 37758944] (WB, Human) WB Human
Sengupta A, Vinayagamurthy S, Soni D et al. Telomeres control regulation of the human Telomerase (hTERT) gene through non-telomeric TRF2 and independent of Telomere looping bioRxiv 2023-10-11 (ChIP, Human) ChIP Human
Roy S, Bagri S, Sengupta A et al. Artificially inserted G-quadruplex DNA secondary structures induce long-distance chromatin activation bioRxiv 2023-11-29 (ChIP, Human) ChIP Human
Zhang K Preserving Genetic Integrity in Reproduction: Insights from Telomere Protection and Sperm Head-Tail Junctions Thesis 2023-01-01
Zhang T, Rawal Y, Jiang H et al. Break-induced replication orchestrates resection-dependent template switching Nature 2023-06-14 [PMID: 37316655]
Pike A, Strong M, Ouyang J et al. TIN2 functions with TPP1/POT1 to stimulate telomerase processivity Cartilage Oct 12 2018 (ICC/IF, Human) ICC/IF Human
Lamm N, Masamsetti V, Read M et al. Nuclear filamentous actin functions in the replication stress response J. Agric. Food Chem. 2018-10-15 (Human) Human
Sullivan DI, Bello FM, Silva AG et al. Intact mitochondrial function in the setting of telomere-induced senescence Aging cell 2023-09-08 [PMID: 37688329] (WB, Mouse) WB Mouse
Show All 136 Publications.

Reviews for TRF-2 Antibody (NB110-57130) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB110-57130:
Filter by Applications
ICC
(1)
ChIP
(1)
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Filter by Species
Human
(1)
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry TRF-2 NB110-57130
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5
reviewed by:
Verified Customer
ICC Human 10/11/2020
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Summary

ApplicationImmunocytochemistry
Sample TestedU-2 OS cells,U2OS cell line
SpeciesHuman
LotD-1

Comments

CommentsAntibody used at 1:400 dilution in antibody dilution buffer and incubated for 2 hours at room-temperature. Detected with Donkey anti-rabbit AF488 antibody.
Chromatin Immunoprecipitation TRF-2 NB110-57130
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5
reviewed by:
Verified Customer
ChIP Mouse 12/01/2014
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Summary

ApplicationChromatin Immunoprecipitation
Sample TestedMouse
SpeciesMouse
LotC-1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRF-2 Antibody (NB110-57130). (Showing 1 - 3 of 3 FAQs).

  1. Are the TRF-2 antibodies validated in Simple Western?
    • Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.
  2. Does TRF-2 antibodies comes in lyophilized form?
    • we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.
  3. What the theoretical molecular weight for TRF-2 antibodies?
    • The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional TRF-2 Products

Research Areas for TRF-2 Antibody (NB110-57130)

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Blogs on TRF-2.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

TRF2: Telomere Maintenance and Protection
TRF2 (telomeric repeat-binding factor 2) is a homodimeric protein that binds the double-stranded 5'-TTAGGG-3' repeat in telomeres. It plays a critical role in the maintenance of telomeres, protecting telomeres against end-to-end chromosomal fusion, an...  Read full blog post.

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Verified Customer
10/11/2020
Application: ICC
Species: Human

Verified Customer
12/01/2014
Application: ChIP
Species: Mouse

Bioinformatics

Gene Symbol TERF2