TERF2IP Antibody

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Western Blot: TERF2IP Antibody [NB100-292] - Detection of Human RAP1 by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry: TERF2IP Antibody [NB100-292] - Samples: FFPE sections of human lung carcinoma.~~ Antibody: Affinity purified rabbit anti-RAP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: TERF2IP Antibody [NB100-292] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinitypurified rabbit anti-RAP1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

TERF2IP Antibody Summary

Immunogen
Antibody was generated using recombinant full length human RAP1 (Telomeric repeat binding factor 2 interacting protein 1) according to entry NP_061848.2
Localization
Telomeres
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TERF2IP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 -1:10000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:500 - 1:2:000
  • Immunohistochemistry-Paraffin 1:500 - 1:2:000
  • Immunoprecipitation 2 - 10 ug/mg of lysate
Application Notes
Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. ICC/IF reactivity reported in scientific literature (PMID: 25271372).
Positive Control
TERF2IP Lysate (NBL1-16816)
Publications
Read Publications using
NB100-292 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TERF2IP Antibody

  • dopamine receptor interacting protein 5
  • Dopamine receptor-interacting protein 5
  • DRIP5
  • hRap1
  • RAP1 homolog
  • RAP1TERF2-interacting telomeric protein 1
  • Repressor/activator protein 1 homolog
  • telomeric repeat binding factor 2, interacting protein
  • telomeric repeat-binding factor 2-interacting protein 1
  • TRF2-interacting telomeric protein 1
  • TRF2-interacting telomeric RAP1 protein

Background

Mammalian telomeric proteins, including TRF1, TRF2, tankyrase, and TERF21P have no recognized orthologs in budding yeast. However, human Rap1 (hRap1) is an ortholog of the yeast telomeric protein, scRap1p. hRap1 is a homodimer, has three conserved sequence motifs in common with scRap1, is located at telomeres, and plays a role in telomere length regulation. Although scRap1 binds telomeric DNA directly, TERF21P is recruited to telomeres by TERF21P and binds it with its C-terminus, rather than directly binding DNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TERF2IP Antibody (NB100-292)(18)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(5)
WB
(1)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB100-292 Applications Species
Schmidt JC, Dalby AB, Cech TR. Identification of human TERT elements necessary for telomerase recruitment to telomeres. Elife. 2014 Nov 03 [PMID: 25271372] (ICC/IF, Human) ICC/IF Human
Kocak H, Ballew BJ, Bisht K et al. Hoyeraal-Hreidarsson syndrome caused by a germline mutation in the TEL patch of the telomere protein TPP1. Genes Dev 2014 Oct 1 [PMID: 25233904] (ICC/IF, Human) ICC/IF Human
Chen LY, Majerska J, Lingner J. Molecular basis of telomere syndrome caused by CTC1 mutations. Genes Dev. 2013 Oct 1 [PMID: 24115768] (ICC/IF, Human) ICC/IF Human
Kamranvar SA, Chen X, Masucci MG. Telomere dysfunction and activation of alternative lengthening of telomeres in B-lymphocytes infected by Epstein-Barr virus. Oncogene 2013 May 27 [PMID: 23708666] (WB, Human) WB Human
Nandakumar J, Bell CF, Weidenfeld I et al. The TEL patch of telomere protein TPP1 mediates telomerase recruitment and processivity Nature 2012 Oct 24 [PMID: 23103865] (ICC/IF, Human) ICC/IF Human
Gu P, Min JN, Wang Y et al. CTC1 deletion results in defective telomere replication, leading to catastrophic telomere loss and stem cell exhaustion. EMBO J 2012 Apr [PMID: 22531781]
Canudas S, Houghtaling BR, Bhanot M et al. A role for heterochromatin protein 1{gamma} at human telomeres. Genes Dev. 2011 Aug 24. [PMID: 21865325]
Choi KH, Farrell AS, Lakamp AS et al. Characterization of the DNA binding specificity of Shelterin complexes. Nucleic Acids Res 2011 Nov [PMID: 21852327]
Yang D, He Q, Kim H et al. TIN2 protein dyskeratosis congenita missense mutants are defective in association with telomerase. J Biol Chem 2011 Jul [PMID: 21536674]
Stohr BA, Blackburn EH. ATM mediates cytotoxicity of a mutant telomerase RNA in human cancer cells. Cancer Res 2008 Jul [PMID: 18593932]
Show All 18 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

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Bioinformatics

Gene Symbol TERF2IP
Entrez
Uniprot