Western Blot: TERF2IP Antibody [NB100-292] - Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAP1 antibody used for WB at 0.1 ug/ml. ...read more
Immunocytochemistry/ Immunofluorescence: TERF21P Antibody [NB100-292] - Localization of RAP1 to Telomeres with TRF2 by Immunofluorescence, using NB 100-292. Sample: HeLa cells.
Immunohistochemistry-Paraffin: TERF2IP Antibody [NB100-292] - FFPE sections of human lung carcinoma. Antibody: Affinity purified rabbit anti-RAP1 used at a dilution of 1:5,000 (0.2 ug/ml).Detection: DAB
Western Blot: TERF21P Antibody [NB100-292] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), and Jurkat (50ug) cells.
Western Blot: TERF21P Antibody [NB100-292] - Sample: Whole cell lysate (30 ug) from HT1080 or IMR90 cells.
Immunohistochemistry-Paraffin: TERF21P Antibody [NB100-292] - Sections of human colon carcinoma.
Immunoprecipitation: TERF21P Antibody [NB100-292] - Detection of Human RAP1 by Western Blot of Immunoprecipitates.
This antibody is useful for Western blot and Immunofluorescence. By Western blot a band at approximately 60 kDa is seen. Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
telomeric repeat binding factor 2, interacting protein
telomeric repeat-binding factor 2-interacting protein 1
TRF2-interacting telomeric protein 1
TRF2-interacting telomeric RAP1 protein
Mammalian telomeric proteins, including TRF1, TRF2, tankyrase, and TERF21P have no recognized orthologs in budding yeast. However, human Rap1 (hRap1) is an ortholog of the yeast telomeric protein, scRap1p. hRap1 is a homodimer, has three conserved sequence motifs in common with scRap1, is located at telomeres, and plays a role in telomere length regulation. Although scRap1 binds telomeric DNA directly, TERF21P is recruited to telomeres by TERF21P and binds it with its C-terminus, rather than directly binding DNA.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.