Tankyrase 1 Antibody

Western blot shows lysates of MCF-7 human breast cancer cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Tankyrase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7116) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tankyrase 1 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Tankyrase inhibition stabilizes AXIN levels and decreases beta -catenin in human HCC cell linesA. HepG2, B. Huh7, and C. Hep40 cells were treated for 24 hours with 10 μM XAV939, 10 μM WXL-8, or DMSO as control, and whole cell extracts isolated for Western blotting using anti-TNKS1, anti-AXIN1, anti-AXIN2, anti-beta -catenin, and anti-survivin. GAPDH was used as the loading control. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26246473), licensed under a CC-BY license. Not internally tested by R&D Systems.

Tankyrase inhibition stabilizes AXIN levels and decreases beta -catenin in human HCC cell linesA. HepG2, B. Huh7, and C. Hep40 cells were treated for 24 hours with 10 μM XAV939, 10 μM WXL-8, or DMSO as control, and whole cell extracts isolated for Western blotting using anti-TNKS1, anti-AXIN1, anti-AXIN2, anti-beta -catenin, and anti-survivin. GAPDH was used as the loading control. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26246473), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Tankyrase 1 Antibody Summary

• Immunogen: E. coli-derived recombinant human Tankyrase 1
  Ala943-Gly1039
  Accession # O95271
• Specificity: Detects human Tankyrase 1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TNKS
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tankyrase 1 Antibody

• EC 2.4.2.30
• PARP5A
• PARP-5a
• PARP5APARPL
• PARPL
• pART5
• Poly [ADP-ribose] polymerase 5A
• TANK1
• Tankyrase 1
• Tankyrase I
• tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
• tankyrase-1
• TIN1TANK1
• TINF1
• TINF1TNKS-1
• TNKS
• TNKS1TRF1-interacting ankyrin-related ADP-ribose polymerase

Background

TNKS (Tankyrase; also TRF1-interacting, ANKYrin-Related ADP-ribose polymerase and TNKS1, TANK1 and PARP5A) is a 165-170 kDa member of the PARP family of proteins. It is ubiquitously expressed and participates in at least two critical cellular functions. First, via ribosylation activity, it inhibits TRF1, a regulator of telomere length. This promotes chromosomal lengthening. Second, it activates the Wnt signaling pathway by promoting Axin-1 and -2 degradation, resulting in beta -catenin activation. Human TNKS is 1327 amino acids (aa) in length. It contains polyHis, Pro and Ser regions (aa 9-134), 15 ankyrin repeats (aa 215-934), one SAM motif (aa 1030-1089) and a PARP catalytic domain (aa 1112-1317). There are three potential splice variants. One shows a three aa substitution for aa 640-1327, another contains a 43 aa substitution for aa 1-327, while a third possesses a 20 aa substitution for aa 300-327. Over aa 943-1039, human TNKS shares 97% aa identity with mouse TNKS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tankyrase 1 Antibody (AF7116)(1)

Publication using AF7116

• Li Ma, Xiaolin Wang, Tao Jia, Wei Wei, Mei-Sze Chua, Samuel So Tankyrase inhibitors attenuate WNT/ beta -catenin signaling and inhibit growth of hepatocellular carcinoma cells Oncotarget 2015-06-27 [PMID: 26246473]

Bioinformatics

• Gene Symbol: TNKS
• Uniprot:
  • O95271(Human)