Rap1A/B Antibody [Unconjugated]

HAF017). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF017). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows recombinant human Rap1A, Rap1B, Rap2A, and Rap2B (1 ng/lane). PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat Rap1A/B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows recombinant human Rap1A, Rap1B, Rap2A, and Rap2B (1 ng/lane). PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat Rap1A/B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band for Rap1A/B was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF017). A specific band for Rap1A/B was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MDA-MB-468 human breast cancer cell line, PT18 mouse mast/basophil cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat Rap1A/B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band for Rap1A/B was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MDA-MB-468 human breast cancer cell line, PT18 mouse mast/basophil cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat Rap1A/B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3767) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." > VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." title="Rap1A/B was detected in immersion fixed paraffin-embedded sections of Human Squamous using Goat Anti-Human/Mouse/Rat Rap1A/B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3767) at 15 µg/mL for 1 hour at room temperature followed by incubation with HRP-DAB. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." />

Rap1A/B was detected in immersion fixed paraffin-embedded sections of Human Squamous using Goat Anti-Human/Mouse/Rat Rap1A/B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3767) at 15 µg/mL for 1 hour at room temperature followed by incubation with HRP-DAB. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Rap1A/B Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Rap1A
  Met1-Leu184
  Accession # P62834
• Specificity: Detects human, mouse and rat Rap1A/B. The antibody is highly specific for Rap1A/B and detects recombinant human Rap2A only upon prolonged film exposure.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RAP1A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rap1A/B Antibody [Unconjugated]

• RAP1A
• Rap1A/B
• RAP1B

Background

The Ras-related proteins Rap1A and Rap1B share 95% identity and together with Rap2A and Rap2B constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Rap proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Rap1 counteracts the mitogenic function of Ras, possibly through competitive interactions with Ras GAPs and Raf, and is implicated in the regulation of integrins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rap1A/B Antibody (AF3767)(4)

Publications using AF3767

• Wang, Y;Tinsley, B;Spolitu, S;Zadroga, JA;Agarwal, H;Sarecha, AK;Ozcan, L; Geranylgeranyl Isoprenoids and Hepatic Rap1a Regulate Basal and Statin-Induced Expression of PCSK9 bioRxiv : the preprint server for biology 2023-10-23 [PMID: 37961667] (Western Blot, Mouse)
• Agarwal, H;Wang, Y;Ozcan, L; Rap1 Activation Protects Against Fatty Liver and Non-Alcoholic Steatohepatitis Development bioRxiv : the preprint server for biology 2023-10-24 [PMID: 37961406] (Western Blot, Human)
• Yating Wang, Stefano Spolitu, John A. Zadroga, Amesh K. Sarecha, Lale Ozcan Hepatocyte Rap1a contributes to obesity- and statin-associated hyperglycemia Cell Reports 8/23/2022 [PMID: 36001955]
• LW Wang, Z Wang, I Ersing, L Nobre, R Guo, S Jiang, S Trudeau, B Zhao, MP Weekes, BE Gewurz Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival PLoS Pathog., 2019-09-13;15(9):e1008030. 2019-09-13 [PMID: 31518366] (Western Blot, Human)

Bioinformatics

• Gene Symbol: RAP1A
• Uniprot:
  • P62834()