Human Holo-Transferrin Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Human Holo-Transferrin Protein, CF Summary

Details of Functionality
Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. Taub, M. et al. (1979) PNAS 76:3338. The ED50 for this effect is 0.075-0.375 μg/mL.
Optimal concentration depends on cell type as well as the application or research objectives.
Source
Human plasma-derived Holo-Transferrin protein

The human plasma used for the isolation of this product were certified by the supplier to be HIV-1 and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.

Protein/Peptide Type
Natural Proteins
Gene
TF
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

SDS-PAGE
76-81 kDa, reducing conditions
Publications
Read Publications using
2914-HT in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in NH4HCO3.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 20 mg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Holo-Transferrin Protein, CF

  • HoloTransferrin
  • Holo-Transferrin

Background

Human Transferrin (Tf) is a single chain, 80 kDa member of the anion-binding superfamily of proteins (1 - 5). It is a bilobed molecule that is the product of an ancient gene duplication event (1, 6). Transferrin is synthesized as a 698 amino acid (aa) precursor that is divided into a 19 aa signal sequence plus a 679 aa mature segment that contains 19 intrachain disulfide bonds. The crystal structure of Tf reveals a protein with two flanking 340 aa globular domains. Each are composed of a beta -sheet surrounded by series of alpha -helices (1, 7). The N- and C-terminal flanking regions (or domains) will bind ferric iron through the interaction of an obligate anion (usually bicarbonate) and four amino acids (His, Asp, and two Tyr) (7, 8). Apotransferrin (or iron‑free) will initially bind one atom of iron at the C-terminus, and this is followed by subsequent iron binding by the N‑terminus to form holotransferrin (diferric Tf) (8, 9). Through its C-terminal iron‑binding domain, holotransferrin will interact with the type I Tf receptor (TfR) on the surface of cells where it is internalized into acidified endosomes. Iron dissociates from the Tf molecule within these endosomes, and is transported into the cytosol as ferrous iron. At physiological pH, iron‑free apotransferrin is not bound by TfR. But at acidic pH, such as exists in the endosome, apotransferrin has considerable affinity for TfR. Thus, it remains bound to TfR and is recycled back to the cell surface where a neutral pH environment dissociates ligand from receptor. Each Tf molecule recycles 100 - 150 times during its lifetime (8 - 11). In addition to TfR, transferrin is reported to bind to cubulin, IGFBP3, microbial iron‑binding proteins and liver-specific TfR2 (7, 12, 13, 14). Transferrin is variably glycosylated and the degree of sialylation is suggestive of certain clinical conditions (15). Finally, Tf is highly allelic and the gene codominant, with many single aa changes noted. Three general forms are known, based on standard electrophoretic mobility. Fast Tf is known as transferrin B, slow transferrin is transferrin D, and the middle migrating transferrin is type/variant C, thre most common (16, 17). Mature human TF is 73% aa identical to both mouse and rat Tf, and 68% and 71% aa identical to bovine and equine Tf, respectively.

  1. Brus, C.M. et al. (2001) Nat. Struct. Biol. 4:919.
  2. Schaeffer, E. et al. (1987) Gene 56:109.
  3. MacGillivray, R.T.A. et al. (1983) J. Biol. Chem. 258:3543.
  4. Yang, F. et al. (1984) Proc. Natl. Acad. Sci. USA 81:2752.
  5. Uzan, G. et al. (1984) Biochem. Biophys. Res. Commun. 119:273.
  6. Zak, O. et al. (2002) Biochemistry 41:7416.
  7. Gomme, P.T. and K. B. McCann (2005) Drug Discov. Today 10:267.
  8. Liu, R. et al. (2003) Biochemistry 42:12447.
  9. Pakdaman, R. et al. (1999) J. Mol. Biol. 293:1273.
  10. Hemadi, M. et al. (2004) Biochemistry 43:1736.
  11. Aisen, P. et al. (2001) Int. J. Biochem. Cell Biol. 33:940.
  12. Kozyraki, R. et al. (2001) Proc. Natl. Acad. Sci. USA 98:12941.
  13. Boulton, I.C. et al. (1998) Biochem. J. 334:269.
  14. Robb, A. and M. Wessling-Resnick (2004) Blood 104:4294.
  15. Landberg, E. et al. (1995) Biochem. Biophys. Res. Commun. 210:267.
  16. Gorg, A. et al. (1983) Hum. Genet. 64:222.
  17. Bean, P. and J.B. Peter (1994) Clin. Chem. 40:2078.

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Publications for Holo-Transferrin (2914-HT)(24)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.


Filter By Application
Bioassay
(9)
Cell Culture
(12)
In Vivo
(2)
All Applications
Filter By Species
Human
(22)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using 2914-HT Applications Species
Z Li, X Zhang, X Li, Y Yang, H Xin, X Yang, N Liu, Z Gai, Y Liu A non-integrated iPSC line (SDQLCHi042-A) from a boy suffering from familial combined hyperlipidemia with compound heterozygous mutations of lipoprotein lipase gene Stem Cell Research, 2021;53(0):102313. 2021 [PMID: 34087978] (Cell Culture, Human) Cell Culture Human
H Zhang, C Liu, Y Ma, L Lin, Y Lv, M Gao, Z Gai, Y Liu Generation of an induced pluripotent stem cell line SDQLCHi026-A from a hereditary tyrosinemia type I patient carrying compound heterozygote mutations in FAH gene Stem Cell Research, 2021;53(0):102331. 2021 [PMID: 33882394] (Bioassay, Human) Bioassay Human
J Walczak, MD Camargo Jo, K Muthumalai Stage Specific Expression Pattern of Alpha-Hemoglobin-Stabilizing-Protein (AHSP) Portrayed in Erythroblast Chronology Methods Protoc, 2020;3(3):. 2020 [PMID: 32629835] (Cell Culture, Human) Cell Culture Human
JF Flatt, CJ Stevens-He, NM Cogan, DJ Eggleston, NM Haines, KJ Heesom, V Picard, C Thomas, LJ Bruce Expression of South East Asian Ovalocytic Band 3 Disrupts Erythroblast Cytokinesis and Reticulocyte Maturation Front Physiol, 2020;11(0):357. 2020 [PMID: 32411010] (Cell Culture, Human) Cell Culture Human
X Tang, X Liu, Z Chen, L Luo, X Liu, J Deng, DW Li, Y Liu Using inducible lentiviral vectors to generate induced pluripotent stem cell line ZOCi001-A from peripheral blood cells of a patient with CRB1-/- retinitis pigmentosa Stem Cell Res, 2020;45(0):101817. 2020 [PMID: 32387899] (Cell Culture, Human) Cell Culture Human
F Di Matteo, F Pipicelli, C Kyrousi, I Tovecci, E Penna, M Crispino, A Chambery, R Russo, AC Ayo-Martin, M Giordano, A Hoffmann, E Ciusani, L Canafoglia, M Götz, R Di Giaimo, S Cappello Cystatin B is essential for proliferation and interneuron migration in individuals with EPM1 epilepsy EMBO Mol Med, 2020;0(0):e11419. 2020 [PMID: 32378798] (Bioassay, Human) Bioassay Human
H Zhang, Y Ma, Y Lv, Y Wan, Q Zhao, Z Gai, Y Liu An integration-free iPSC line SDQLCHi025-A from a girl with multiminicore disease carrying compound heterozygote mutations in RYR1 gene Stem Cell Res, 2020;45(0):101775. 2020 [PMID: 32272370] (Bioassay, Human) Bioassay Human
X Liu, X Yang, Y Li, X Wang, J Ma, W Jiang, Y Liu, W Sun, Y Gong Generation of patient-specific pluripotent induced stem cell line SDUBMSI002-A from a patient with X-linked mental retardation syndrome Stem Cell Res, 2020;43(0):101724. 2020 [PMID: 32078988] (Human) Human
Y Ma, H Zhang, X Li, X Yang, Y Li, J Guan, Y Lv, Z Gai, Y Liu An integration-free iPSC line (SDQLCHi017-A) derived from a patient with nemaline myopathy-2 disease carrying compound heterozygote mutations in NEB gene Stem Cell Res, 2020;43(0):101729. 2020 [PMID: 32062132] (Cell Culture, Human) Cell Culture Human
J Guan, Z Li, H Zhang, X Yang, Y Ma, Y Li, R Dong, Z Gai, Y Liu Generation of a Human iPSC line (SDQLCHi021-A) from a patient with methylmalonic acidemia cblC type carrying compound heterozygous mutations in MMAHC gene Stem Cell Res, 2020;43(0):101709. 2020 [PMID: 32058304] (Cell Culture, Human) Cell Culture Human
Show All 24 Publications.

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Bioinformatics

Gene Symbol TF