TLR9 Antibody (26C593.2) [FITC]

Product Discontinued

TLR9 Antibody (26C593.2) [FITC] Summary

• Immunogen: This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLR9
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1ul/1 million cells
• Application Notes: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases.
• Agonist:
  • CpG oligodeoxynucleotides with negative control, TLR9 ligand NBP2-26232

Reactivity Notes

Rhesus Monkey.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for TLR9 Antibody (26C593.2) [FITC]

• CD289 antigen
• CD289
• TLR9
• toll-like receptor 9

Background

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR9 Antibody (NBP2-24908)(57)

Publications using NBP2-24908

• Jamin A, Dehoux L, Dossier C et al. Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome Clin Exp Immunol. [PMID: 26123900] (FLOW, Human)
• Saito K, Kukita K, Kutomi G et al. Heat shock protein 90 associates with Toll-like receptors 7/9 and mediates self-nucleic acid recognition in SLE Eur J Immunol 2015-04-13 [PMID: 25871979] (ICC/IF, Human, Mouse)
  
  Details:
  This citation used the FITC version of this antibody.
• Belmont L, Rabbe N, Antoine M et al. Expression of TLR9 in tumor-infiltrating mononuclear cells enhances angiogenesis and is associated with a worse survival in lung cancer. Int J Cancer. 2013-08-03 [PMID: 23913633]
• Figueiredo MM, Amorim IF, Pinto AJ et al. Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum. BMC Immunol. 2013-05-14 [PMID: 23668673]
• Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013-04-08 [PMID: 23593278]
  
  Details:
  Antibodies cited [multiple myeloma (MM) cell lines, human intestine tissue lysate, PBMC, mouse splenocytes] for WB (Figs 2, 3, 4A, 4B, 5, Table 1) and Flow (Intracellular): Figs 4C, 6-8, Table 1: 1. TLR1 (IMG-5012) 2. TLR2 (IMG-416A)3. TLR3 (IMG-315A)4 TL
• Chauhan SK, Singh VV, Rai R et al. Distinct autoantibody profiles in systemic lupus erythematosus patients are selectively associated with TLR7 and TLR9 upregulation. J Clin Immunol. 2013-07-01 [PMID: 23564191]
  
  Details:
  PBLs (WB) or B-cells (Flow intracellular) from SLE patients or controls: 1. TLR7 mAb clone IMG4G6 (IMG-6675A): WB (Figs 2a, 2c).
• Hu F, Zhang L, Zheng J et al. Spontaneous production of immunoglobulin M in human epithelial cancer cells. PLoS One. 2012-01-01 [PMID: 23251529]
• Harman AN, Bye CR, Nasr N et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013-01-01 [PMID: 23183897]
  
  Details:
  Antibodies cited in Fig 4B for flow cytometric analysis of TLR expression in human monocyte-derived dendritic cells (MDDC), CD14+ monocytes, myeloid DC, and plasmacytoid DC:1. TLR2-FITC, clone T2.1 (IMG-416C): Flow (cell surface)2. TLR4-PE, clone HTA124 (
• Siponen M, Kauppila JH, Soini Y, Salo T. TLR4 and TLR9 are induced in oral lichen planus. J Oral Pathol Med. 2012-11-01 [PMID: 22672741]
• Souza-Fonseca-Guimaraes F, Parlato M, Fitting C et al. NK cell tolerance to TLR agonists mediated by regulatory T cells after polymicrobial sepsis. J Immunol. 2012-06-15 [PMID: 22566566]
• Silver AC, Arjona A, Walker WE, Fikrig E. The circadian clock controls toll-like receptor 9-mediated innate and adaptive immunity. Immunity. 2012-02-24 [PMID: 22342842]
• Zuo D, Yu X, Guo C et al. Molecular chaperoning by glucose-regulated protein 170 in the extracellular milieu promotes macrophage-mediated pathogen sensing and innate immunity. FASEB J. 2012-04-01 [PMID: 22207611]
• Karim R, Meyers C, Backendorf C et al. Human papillomavirus deregulates the response of a cellular network comprising of chemotactic and proinflammatory genes. PLoS One. 2011-03-14 [PMID: 21423754]
• Dasgupta G, Chentoufi AA, You S et al. Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses. Invest Ophthalmol Vis Sci. 2011-05-17 [PMID: 21273544]
  
  Details:
  Products cited: Flow (intracellular), Fig 1A ( CD4+CD25+ regulatory T cells purified from rabbit conjunctiva) & Fig 2A (CD11b+ cells isolated from rabbit conjunctiva): TLR2/CD282 PE (IMG-416D), TLR3/CD283 (IMG-315D), TLR4/CD284 (IMG-417D), TLR8/CD288 (IMG
• van Gent M, Griffin BD, Berkhoff EG et al. EBV lytic-phase protein BGLF5 contributes to TLR9 downregulation during productive infection. J Immunol. 2011-02-01 [PMID: 21191071]
  
  Details:
  TLR9/CD289 (IMG-305A). WB: Ak31 and AkBM cell postnuclear lysates infected with Epstein-Barr virus, Fig 2B. WB: TLR9 transfected 293T cells, Fig 4D. Note: The TLR9 antibody (IMG-305A) was transfected validated by WB in 293T cells, Fig 4D.
• Myles A, Aggarwal A. Expression of Toll-like receptors 2 and 4 is increased in peripheral blood and synovial fluid monocytes of patients with enthesitis-related arthritis subtype of juvenile idiopathic arthritis. Rheumatology (Oxford). 2011-03-01 [PMID: 21097451]
• Schneberger D, Lewis D, Caldwell S, Singh B. Expression of toll-like receptor 9 in lungs of pigs, dogs and cattle. Int J Exp Pathol. 2011-02-01 [PMID: 21044185]
• Sethi RS, Brar RS, Singh O, Singh B. Immunolocalization of pulmonary intravascular macrophages, TLR4, TLR9 and IL-8 in normal and Pasteurella multocida-infected lungs of water buffalo (Bubalus bubalis). J Comp Pathol. 2011-02-01 [PMID: 20880542]
  
  Details:
  TLR9 (IMG-305A) for IHC (P): Fig 5a-f (water buffalo lung lungs). Inflamed lung showed increased TLR9 expression in the alveolar septa, airway epithelium, vascular endothelium, and blood cells as compared to normal lung.
• Wang C, Cao S, Yan Y et al. TLR9 expression in glioma tissues correlated to glioma progression and the prognosis of GBM patients. BMC Cancer. 2010-08-10 [PMID: 20696081]
• Booth JS, Arsenault R, Napper S et al. TLR9 signaling failure renders Peyer's patch regulatory B cells unresponsive to stimulation with CpG oligodeoxynucleotides. J Innate Immun. 2010-01-01 [PMID: 20551621]
• Keestra AM, de Zoete MR, Bouwman LI, van Putten JP. Chicken TLR21 is an innate CpG DNA receptor distinct from mammalian TLR9. J Immunol. 2010-07-01 [PMID: 20498358]
  
  Details:
  Confocal microscopy: HEk293 cells transfacted with hTLR9, Fig 6A. Note: Monoclonal TLR9 antibody was transfected validated in TLR9 transfected HEk293 cells by confocal microscopy, Fig 6A.
• Jukkola-Vuorinen A, Rahko E, Vuopala KS et al. Toll-like receptor-9 expression is inversely correlated with estrogen receptor status in breast cancer. J Innate Immun. 2009-01-01 [PMID: 20375566]
• Machida H, Ito S, Hirose T et al. Expression of Toll-like receptor 9 in renal podocytes in childhood-onset active and inactive lupus nephritis. Nephrol Dial Transplant. 2010-08-01 [PMID: 20181802]
  
  Details:
  TLR9 (IMG-305A) IHC (paraffin), Fig 2A, human normal kidney control. Fig 2B, Fig 2C, active lupus kidney.
• Kim YI, Park JE, Brand DD et al. Protein kinase D1 is essential for the proinflammatory response induced by hypersensitivity pneumonitis-causing thermophilic actinomycetes Saccharopolyspora rectivirgula. J Immunol. 2010-03-15 [PMID: 20142359]
  
  Details:
  TLR9 (IMG-305A) WB, Fig 2, lung cells from MyD88 deficient mice.
• Panda A, Qian F, Mohanty S et al. Age-associated decrease in TLR function in primary human dendritic cells predicts influenza vaccine response. J Immunol. 2010-03-01 [PMID: 20100933]
• Chentoufi AA, Dasgupta G, Nesburn AB et al. Nasolacrimal duct closure modulates ocular mucosal and systemic CD4(+) T-cell responses induced following topical ocular or intranasal immunization. Clin Vaccine Immunol. 2010-03-01 [PMID: 20089796]
• Wu J, Meng Z, Jiang M et al. Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Immunology. [PMID: 19922426]
  
  Details:
  Citation using the PE/Cy5 form of this antibody.
• Zhou M, McFarland-Mancini MM, Funk HM et al. Toll-like receptor expression in normal ovary and ovarian tumors. Cancer Immunol Immunother. 2009-09-01 [PMID: 19184006]
• Palladino Michael A, Savarese Michael A, Chapman Jessica L et al. Localization of Toll-like receptors on epididymal epithelial cells and spermatozoa. Am J Reprod Immunol. 2008-12-01 [PMID: 19032616]
• Sacre SM, Lo A, Gregory B et al. Inhibitors of TLR8 reduce TNF production from human rheumatoid synovial membrane cultures. J Immunol. 2008-12-01 [PMID: 19017992]
• Meneghin A, Choi ES, Evanoff HL et al. TLR9 is expressed in idiopathic interstitial pneumonia and its activation promotes in vitro myofibroblast differentiation. Histochem Cell Biol. 2008-11-01 [PMID: 18633634]
• Cohen PA, Koski GK, Czerniecki BJ et al. STAT3- and STAT5-dependent pathways competitively regulate the pan-differentiation of CD34pos cells into tumor-competent dendritic cells. Blood. 2008-09-01 [PMID: 18577706]
  
  Details:
  Flow (intracellular), mouse bone marrow cells, Fig. 1E: 1. TLR3 FITC (IMG-315C) 2. TLR4 FITC (IMG-417C) 3. TLR7 (IMG-665A) 4. TLR8 FITC (IMG-321C) 5. TLR9 FITC (IMG-305C).
• Selleri S, Palazzo M, Deola S et al. Induction of pro-inflammatory programs in enteroendocrine cells by the Toll-like receptor agonists flagellin and bacterial LPS. Int Immunol. 2008-08-01 [PMID: 18544573] (Flow Cytometry Control, Human)
  
  Details:
  TLR9-FITC (IMG-305C): Flow (intracellular), Human colon neuroendocrine LCC-18 cell line: Fig. 4d.
• Ju X, Zenke M, Hart DN, Clark GJ. CD300a/c regulate type I interferon and TNF-alpha secretion by human plasmacytoid dendritic cells stimulated with TLR7 and TLR9 ligands. Blood. 2008-08-15 [PMID: 18535206]
• Hammadi A, Billard C, Faussat AM et al. Stimulation of iNOS expression and apoptosis resistance in B-cell chronic lymphocytic leukemia (B-CLL) cells through engagement of Toll-like receptor 7 (TLR-7) and NF-kappaB activation. Nitric Oxide. 2008-09-01 [PMID: 18474259]
• Zhang YW, Davis EG, Blecha F, Wilkerson MJ. Molecular cloning and characterization of equine Toll-like receptor 9. Vet Immunol Immunopathol. 2008-08-15 [PMID: 18462806]
• Cognasse F, Hamzeh-Cognasse H, Lafarge S et al. Identification of two subpopulations of purified human blood B cells, CD27- CD23+ and CD27high CD80+, that strongly express cell surface Toll-like receptor 9 and secrete high levels of interleukin-6. Immunology. 2008-11-01 [PMID: 18445007]
• Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008-07-15 [PMID: 18439687]
  
  Details:
  Canine (dog) PBMC subpopulations: 1. 10083K [IC-Flow (Intracellular Staining Flow Assay) Kit.
• Nakamura K, Miyazato A, Xiao G et al. Deoxynucleic acids from Cryptococcus neoformans activate myeloid dendritic cells via a TLR9-dependent pathway. J Immunol. 2008-03-15 [PMID: 18322216]
  
  Details:
  TLR9-FITC (IMG-305C) for IF/ICC (intracellular confocal microscopy) in the following figures: 1. Fig 8a: primary mouse bone marrow-derived myeloid dendritic cells (BM-DC) treated with Cn-DNA and CpG-Rhodamine and analyzed over a time course 2. Fig 8b: BM-DC and RAW264.1 cells treated with Cn-DNA Note: intracellular redistribution of TLR9 was observed in treated cells.
• Pegu A, Qin S, Fallert Junecko BA et al. Human lymphatic endothelial cells express multiple functional TLRs. J Immunol. 2008-03-01 [PMID: 18292566]
  
  Details:
  Antibodies cited [Flow (Cell surface) and Flow (Intracellular), human lymphatic endothelial cells, Fig. 1D]: 1. IMG-663C (TLR5-FITC) 2. IMG-304C (TLR6-FITC).
• Meng Y, Kujas M, Marie Y et al. Expression of TLR9 within human glioblastoma. J Neurooncol. 2008-05-01 [PMID: 18253698] (IHC-P, Human)
• Nakano S, Morimoto S, Suzuki J et al. Role of pathogenic auto-antibody production by Toll-like receptor 9 of B cells in active systemic lupus erythematosus. Rheumatology (Oxford). 2008-02-01 [PMID: 18160420] (Flow Cytometry Control, Human)
• Tran N, Koch A, Berkels R et al. Toll-like receptor 9 expression in murine and human adrenal glands and possible implications during inflammation. J Clin Endocrinol Metab. 2007-07-01 [PMID: 17473064]
• Kindrachuk J, Potter JE, Brownlie R et al. Nucleic acids exert a sequence-independent cooperative effect on sequence-dependent activation of Toll-like receptor 9. J Biol Chem. 2007-05-11 [PMID: 17355967]
  
  Details:
  Product cited: TLR9 (IMG-305A) 1. WB, Fig 1: TLR9 stably transfected bovine kidney epithelial cells, these cells secrete recombinant TLR9 (ligand binding domain) into the media. The data shows the molecular weight of TLR9 was reduced following deglycosyla
• Wong CK, Cheung PF, Ip WK et al. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007-07-01 [PMID: 17332440] (Flow Cytometry Control, Flow-CS, Human)
  
  Details:
  Human blood eosinophils and neutrophils from buffy coat: For WB, Fig. 1A: TLR1 (IMG-5012), TLR5 (IMG-664), TLR6 (IMG-304A), TLR7 (IMG-540), TLR8 (IMG-321A), TLR9 (IMG-305A). For Flow (Intracellular) and Flow (Surface), Fig. 1B: TLR1 (IMG-5021), TLR2 (IMG-416C), TLR3 (IMG-315C), TLR4 (IMG-417C), TLR5 (IMG-663C), TLR6 (IMG-304C), TLR7 (IMG-665A), TLR8 (IMG-321C), TLR9 (IMG-305C).
• Ewaschuk JB, Backer JL, Churchill TA et al. Surface expression of Toll-like receptor 9 is upregulated on intestinal epithelial cells in response to pathogenic bacterial DNA. Infect Immun. 2007-05-01 [PMID: 17325049]
• Palladino M A, Johnson T A, Gupta R et al. Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract. Biol Reprod. 2007-06-01 [PMID: 17314314]
• Lee J, Mo JH, Katakura K et al. Maintenance of colonic homeostasis by distinctive apical TLR9 signalling in intestinal epithelial cells. Nat Cell Biol. 2006-12-01 [PMID: 17128265] (Flow Cytometry Control)
• Huggins J, Pellegrin T, Felgar RE et al. CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells Blood 2007-02-15 [PMID: 17032927] (FLOW)
  
  Details:
  This citation used the FITC version of this antibody.
• Wang H, Nicholas MW, Conway KL et al. EBV latent membrane protein 2A induces autoreactive B cell activation and TLR hypersensitivity J Immunol 2006-09-01 [PMID: 16920914] (FLOW)
  
  Details:
  This citation used the FITC version of this antibody.
• Mansson A, Adner M, Cardell LO. Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis. Respir Res. 2006-02-27 [PMID: 16504163] (Flow Cytometry Control, Human)
  
  Details:
  Antibodies cited (human tonsils separated into cell subtypes): 1. TLR3 [IMG-315D (Flow-Intracellular), Figs 5 and 6]. 2. TLR5 [IMG-663A (Flow-Intracellular), Fig 6]. 3. TLR9 [IMG-305C (Flow-Intracellular), Fig 4.].
• Lee J, Wu CC, Lee KJ et al. Activation of anti-hepatitis C virus responses via Toll-like receptor 7. Proc Natl Acad Sci U S A. 2006-02-07 [PMID: 16446426]
• Miller LS, SOrensen OE, Liu PT et al. TGF-alpha regulates TLR expression and function on epidermal keratinocytes. J Immunol. 2005-05-15 [PMID: 15879109]
• Abel K, Wang Y, Fritts L et al. Deoxycytidyl-deoxyguanosine oligonucleotide classes A, B, and C induce distinct cytokine gene expression patterns in rhesus monkey peripheral blood mononuclear cells and distinct alpha interferon responses in TLR9-expressing rhesus monkey plasmacytoid den Clin Diagn Lab Immunol. 2005-05-01 [PMID: 15879022] (Flow Cytometry Control, Primate (Rhesus monkey))
  
  Details:
  IMG-305C [Flow (Intracellular), Fig. 5] on Rhesus monkey spleen cell suspensions and PBMC.
• Greene CM, Carroll TP, Smith SG et al. TLR-induced inflammation in cystic fibrosis and non-cystic fibrosis airway epithelial cells. J Immunol. 2005-02-01 [PMID: 15661927]
  
  Details:
  Functional Assays: 1.TLR2/NF-kB/SEAP Stably Transfected HEK 293 Cells (IML-102). 2. TLR4/MD-2/NF-kB/SEAP Stably Transfected HEK 293 Cells (IML-104MD2). 3. TLR4/NF-kB/SEAP Stably Transfected HEK 293 Cells (IML-104).4.TLR7/NF-kB/SEAP Stably Transfected HEK 293 Cells (IML-107). 5. LPS from E. Coli, TLR4 Ligand (IMG-2204). 6. SEAPorter Assay Kit (10055K).
• Zhong F, Cao W, Chan E et al. Deviation from major codons in the Toll-like receptor genes is associated with low Toll-like receptor expression Immunology 2005-01-01 [PMID: 15606798] (FLOW)
  
  Details:
  This citation used the FITC version of this antibody.
• Lee HK, Dunzendorfer S, Tobias PS. Cytoplasmic domain-mediated dimerizations of toll-like receptor 4 observed by beta-lactamase enzyme fragment complementation. J Biol Chem. 2004-03-12 [PMID: 14699116] (WB)
  
  Details:
  Antibodies cited: TLR9 (IMG-305) [WB, Fig5B (HEK293 cells)].

Bioinformatics

• Gene Symbol: TLR9