Flow Cytometry: Human TLR7 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26278] - Intracellular staining of TLR7 in the TLR7 reporter cell line was analyzed by flow cytometry using PE-conjugated ...read more
Ligand Activation: Human TLR7 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26278] - The TLR7 reporter cell line was plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated ...read more
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Human TLR7 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line Summary
The TLR7 reporter line can be used for TLR7-dependent functional assays as well as screening of TLR7 agonists or antagonists. The TLR7 reporter cell line has been validated by flow cytometry (fig. 2) and ligand dose response assay (fig. 1).
Contents: 3~4 x 10^6 cells
Biosafety Level: 2
The TLR7 reporter cell line is a stably co-transfected cell line which expresses full-length human Toll-like receptor7 (TLR7) and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element.
Blasticidin and G418.
Adherent Morphology: Epithelial
NF-kB/SEAP - (SEAPorter™)
Secreted alkaline phosphatase (SEAP)
Use in In vitro assay reported in scientific literature (PMID 26193334)
Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed. - Use pipette aids to prevent ingestion and keep aerosols down to a minimum. - No eating, drinking or smoking while handling the TLR7 reporter line. - Wash hands after handling the TLR7 reporter line and before leaving the lab. - Decontaminate work surface with disinfectant or 70% ethanol before and after working with cells. - All waste should be considered hazardous. - Dispose of all liquid waste after each experiment and treat with bleach.
Alternate Names for Human TLR7 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line
toll-like receptor 7
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.
FAQs for TLR7 Reporter Cell Line (NBP2-26278). (Showing 1 - 1 of 1 FAQs).
What is the construct for this cell line and how many NF-Kb binding sites are there?
The only data I am able to find on this particular cell line is in the publications listed on the datasheet: TLR7 Reporter Cell Line (NBP2-26278) Publications Section. Unfortunately, we cannot offer the construct for this cell line. This cell line is proprietary to Novus Biologicals, and I am not able to offer any further information. I apologize for any inconvenience.
TLR9: For Whom the Cell Tolls The Toll-like receptor 9 (TLR9) protein, also known as CD289, belongs to the family of Toll-like receptor (TLR) proteins which play a large role in pathogen recognition and the activation of innate immunity. Scientists using TLR9 antibodies have found... Read full blog post.
TLR7 and Immune Response Regulation Toll-like receptor 7 (TLR7) is a protein encoded by the TLR7 gene in humans and is a member of TLR family. TLRs controls host immune response against pathogens (e.g. viruses, bacteria and fungi) through recognition of pathogen-associated molecular pat... Read full blog post.
TLR9 Antibodies in Immunity Research Toll-like receptor 9 (TLR9) is a member of the toll-like receptor family that plays a key role in pathogen recognition and activation of innate immunity. Scientists using TLR9 antibodies have found the protein is highly conserved from Drosophila to hu... Read full blog post.