Human TLR7 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line

Images

 
Flow Cytometry: Human TLR7 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26278] - Intracellular staining of TLR7 in the TLR7 reporter cell line was analyzed by flow cytometry using PE-conjugated ...read more
Ligand Activation: Human TLR7 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26278] - The TLR7 reporter cell line was plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated ...read more

Product Details

Summary
Applications Flow, In vitro, LA
Host
HEK293

Order Details

Human TLR7 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line Summary

Description
The TLR7 reporter line can be used for TLR7-dependent functional assays as well as screening of TLR7 agonists or antagonists. The TLR7 reporter cell line has been validated by flow cytometry (fig. 2) and ligand dose response assay (fig. 1).

Contents: 3~4 x 10^6 cells
Biosafety Level: 2
Immunogen
The TLR7 reporter cell line is a stably co-transfected cell line which expresses full-length human Toll-like receptor7 (TLR7) and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element.
Target Species
Human
Specificity
TLR7/NF-kB/SEAP
Selection Agent
Blasticidin and G418.
Growth Properties
Adherent Morphology: Epithelial
RCL Type
NF-kB/SEAP - (SEAPorter™)
Reporter Gene
Secreted alkaline phosphatase (SEAP)
Host
HEK293
Gene
TLR7

Applications/Dilutions

Application Notes
Use in In vitro assay reported in scientific literature (PMID 26193334)
Agonist
Readout System
Publications
Read Publications using NBP2-26278.

Packaging, Storage & Formulations

Storage
Store in gas phase of liquid nitrogen.
Reconstitution Instructions
Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 10 ug/ml blasticidin + 0.5 mg/ml G418 (Geneticin).

Details for TLR7

Type
Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the TLR7 reporter line.
- Wash hands after handling the TLR7 reporter line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with cells.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Human TLR7 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line

  • TLR7
  • toll-like receptor 7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

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Publications for TLR7 Reporter Cell Line (NBP2-26278)(5)

We have publications tested in 1 application: In vitro.


Filter By Application
In vitro
(1)
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Product General Protocols

View specific protocols for TLR7 Reporter Cell Line (NBP2-26278): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TLR7 Reporter Cell Line (NBP2-26278). (Showing 1 - 1 of 1 FAQs).

  1. What is the construct for this cell line and how many NF-Kb binding sites are there?
    • The only data I am able to find on this particular cell line is in the publications listed on the datasheet: TLR7 Reporter Cell Line (NBP2-26278) Publications Section. Unfortunately, we cannot offer the construct for this cell line. This cell line is proprietary to Novus Biologicals, and I am not able to offer any further information. I apologize for any inconvenience.

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Bioinformatics

Gene Symbol TLR7
Entrez