Intracellular Staining Kit

Flow Cytometry: Intracellular Staining Kit [NBP2-26248] - TLR3 in human PBMCs using anti-TLR3 monoclonal antibody and Intracellular Staining Flow Kit. The shaded histogram represents cells without antibody; green represents an isotype control; red represents anti-TLR3 antibody.

Product Discontinued

Intracellular Staining Kit Summary

• Description: This kit is optimized for intracellular staining of cells in flow cytometric or immunocytochemical applications. It is designed and optimized to minimize non-specific staining while maximizing signal-to-noise ratio for clear and consistent data.
• Kit Type: Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Fixation buffer is used to "fix" the cells/cellular proteins prior to permeabilization. Permeabilization buffer permeabilizes the cell membrane, allowing the detecting antibodies access to intracellular proteins.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Alternate Names for Intracellular Staining Kit

• Permeabilization buffer

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Intracellular Staining Kit (NBP2-26248)(3)

Publications using NBP2-26248

• Nasr AS, Fawzy SM, Gheita TA, El-Khateeb E. Expression of Toll-like receptors 3 and 9 in Egyptian systemic lupus erythematosus patients. Z Rheumatol. 2015-12-17 [PMID: 26680366] (FLOW)
• Klonowska-Szymczyk A, Wolska A, Robak T. Expression of Toll-Like Receptors 3, 7, and 9 in Peripheral Blood Mononuclear Cells from Patients with Systemic Lupus Erythematosus. Mediators Inflamm 2014-04-03 [PMID: 24692849]
• Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008-07-15 [PMID: 18439687] (Flow Cytometry Control, Canine)
  
  Details:
  Canine (dog) PBMC subpopulations: 1. 10083K [IC-Flow (Intracellular Staining Flow Assay) Kit.