Human TLR7 Stable Cell Line

Images

 
Western Blot: Human TLR7 Stable Cell Line [NBP2-26270] - analysis of TLR7 expression in the TLR7 stable cell line using an HA antibody (20 ug total protein/lane). Legend. Vect: Vector control stable cell line; TLR7: ...read more
Flow Cytometry: Human TLR7 Stable Cell Line [NBP2-26270] - analysis of TLR7 expression in the TLR7 stable cell line. Intracellular expression of TLR7 in the TLR7 stable cell line was analyzed by flow cytometry using a ...read more
Functional: Human TLR7 Stable Cell Line [NBP2-26270] - Functional analysis of the TLR7 stable cell line. The assay was performed using the NF-kB SEAPorter™ Assay Kit (NBP2-25286). The Vector control stable cell ...read more

Product Details

Summary
Host
HEK293

Order Details

Human TLR7 Stable Cell Line Summary

Description
The TLR7 stable cell line can be used for TLR7 flow cytometric calibration and detection control as well as TLR7-dependent functional assays. TLR7 expression in this stable cell line has been validated by Western blotting (fig. 1) and flow cytometry (fig. 2). Functional activity of this stable cell line has been validated using the NF-kB/SEAPorter™ Assay Kit (NBP2-25286, fig. 3).

Contents: 3~4 x 10^6 cells
Biosafety Level: 2
Immunogen
The TLR7 stable cell line is a stably transfected cell line which expresses full-length human Toll-like receptor 7 (TLR7) with an N-terminal HA tag.
Target Species
Human
Specificity
TLR7
Selection Agent
Blasticidin
Growth Properties
Adherent Morphology: Epithelial
RCL Type
Stable Cell Line
Host
HEK293
Gene
TLR7

Applications/Dilutions

Packaging, Storage & Formulations

Storage
Store in gas phase of liquid nitrogen.
Reconstitution Instructions
Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 10 ug/ml blasticidin.

Details for TLR7

Type
Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the stable line.
- Wash hands after handling the stable line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with cells.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Human TLR7 Stable Cell Line

  • TLR7
  • toll-like receptor 7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

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Publications for TLR7 Reporter Cell Line (NBP2-26270)(1)

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Product General Protocols

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TLR7 and Immune Response Regulation
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Bioinformatics

Gene Symbol TLR7
Entrez