TLR6 Antibody (86B1153.2) [PE]

Flow Cytometry: TLR6 Antibody (86B1153.2) [PE] [NBP2-24969] - Intracellular analysis of TLR6 in Ramos cells using this antibody. 5 ug/10^6 cells. Shaded histogram represents Ramos cells without antibody; green represents isotype control (BD Pharmingen); red represents anti-TLR6 antibody.

Flow Cytometry: TLR6 Antibody (86B1153.2) [PE] [NBP2-24969] - Intracellular analysis of TLR6 in human PBMC cells using this antibody at 0.5 ug/10^6 cells. Shaded histogram represents PBMC without antibody; green represents isotype control (BD Pharmingen); red represents this antibody.

• Reactivity: Hu
• Applications: Flow
• Clone: 86B1153.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

TLR6 Antibody (86B1153.2) [PE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: This antibody was developed against a synthetic peptide corresponding to amino acids 408-424 of human TRL6.
• Specificity: TLR6 (86B1153.2)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLR6
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1ul/1 million cells
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB100-56536. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
• Agonist:
  • MALP-2, TLR6 and TLR2 ligand NBP2-26219

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for TLR6 Antibody (86B1153.2) [PE]

• CD286 antigen
• CD286
• TLR6
• toll-like receptor 6

Background

TLR6 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR6 functionally interacts with toll-like receptor 2 to mediate cellular response to bacterial lipoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR6 Antibody (NBP2-24969)(7)

Publications using NBP2-24969

• Oberg HH, Ly TT, Ussat S et al. Differential but direct abolishment of human regulatory T cell suppressive capacity by various TLR2 ligands. J Immunol. 2010-05-01 [PMID: 20363971]
  
  Details:
  Flow(intracellular) cytometry: TLR2/CD282 PE (IMG-416D) and TLR6/CD286 (IMG-304A). T cells and Tregs were separated from freshly isolated human PBMCs, Fig S3.
• van den Berk LC, Jansen BJ, Siebers-Vermeulen KG et al. Toll-like receptor triggering in cord blood mesenchymal stem cells. J Cell Mol Med. 2009-09-01 [PMID: 20196781] (Human)
  
  Details:
  flow (cell surface) cytometry: TLR5 (IMG-663A), TLR6 (IMG-304), TLR8 (IMG-321). Human mesenchymal stem cells, Fig 1C.
• Pietschmann K, Beetz S, Welte S et al. Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes. Scand J Immunol. 2009-09-01 [PMID: 19703014]
• Pegu A, Qin S, Fallert Junecko BA et al. Human lymphatic endothelial cells express multiple functional TLRs. J Immunol. 2008-03-01 [PMID: 18292566]
  
  Details:
  Antibodies cited [Flow (Cell surface) and Flow (Intracellular), human lymphatic endothelial cells, Fig. 1D]: 1. IMG-663C (TLR5-FITC) 2. IMG-304C (TLR6-FITC).
• Peiser M, Koeck J, Kirschning CJ et al. Human Langerhans cells selectively activated via Toll-like receptor 2 agonists acquire migratory and CD4+T cell stimulatory capacity. J Leukoc Biol. 2008-05-01 [PMID: 18252867]
  
  Details:
  IMG-304A Flow (Cell Surface), Fig. 1C [primary human Langerhans cells (LC), LC cells generated from monocytes, and dendritic cells generated from monocytes].
• Murciano C, Villamon E, Yanez A et al. In vitro response to Candida albicans in cultures of whole human blood from young and aged donors. FEMS Immunol Med Microbiol. 2007-11-01 [PMID: 17714490]
  
  Details:
  The following anitbodies were used in flow (cell surface) cytometry: TLR2/CD282 FITC (IMG-416C), TLR4 FITC (IMG-417C), TLR6/CD286 FITC (IMG-304C). Human whole blood from both young and elderly volunteers, Fig 1. Note: The fluorescence mean intensity was m
• Wong CK, Cheung PF, Ip WK et al. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007-07-01 [PMID: 17332440]
  
  Details:
  Human blood eosinophils and neutrophils from buffy coat: For WB, Fig. 1A: TLR1 (IMG-5012), TLR5 (IMG-664), TLR6 (IMG-304A), TLR7 (IMG-540), TLR8 (IMG-321A), TLR9 (IMG-305A). For Flow (Intracellular) and Flow (Surface), Fig. 1B: TLR1 (IMG-5021), TLR2 (IMG-416C), TLR3 (IMG-315C), TLR4 (IMG-417C), TLR5 (IMG-663C), TLR6 (IMG-304C), TLR7 (IMG-665A), TLR8 (IMG-321C), TLR9 (IMG-305C).

Bioinformatics

• Gene Symbol: TLR6