TLR5 Antibody (19D759.2) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, Flow, Flow-CS, Flow-IC, IHC-Fr, IHC-P
Clone
19D759.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

TLR5 Antibody (19D759.2) [Alexa Fluor® 488] Summary

Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 between amino acids 700-800. The antibody also reacts with mouse TLR5.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TLR5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Flow (Cell Surface)
  • Flow (Intracellular)
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Agonist
Reviewed Applications
Read 1 Review rated 5
using
NBP2-24787AF488 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for TLR5 Antibody (19D759.2) [Alexa Fluor® 488]

  • MGC126430
  • MGC126431
  • SLEB1FLJ10052
  • TIL-3
  • TIL3Toll/interleukin-1 receptor-like protein 3
  • TLR5
  • toll-like receptor 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for TLR5 Antibody (NBP2-24787AF488) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NBP2-24787AF488:
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ICC
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry TLR5 NBP2-24787AF488
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reviewed by:
Anonymous
ICC Human 05/30/2017
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Summary

ApplicationImmunocytochemistry
Sample Testedbreast cancer MCF7 cells
SpeciesHuman

Comments

CommentsCells were cultivated on L-lysine coated coverslips in DMEM/F12 suppl. 10% FBS medium, fixed for 10 minutes with neutral buffered formalin 2% at room temperature and incubated with anti-EpCAM AF488-conjugated antibody in BSA 3%-TBS 1X buffer, over night at +4°C in a humidified chamber.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for TLR5 Antibody (NBP2-24787AF488) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 647 Labeled NBP2-24787AF647
Alexa Fluor 700 Labeled NBP2-24787AF700

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Bioinformatics Tool for TLR5 Antibody (NBP2-24787AF488)

Discover related pathways, diseases and genes to TLR5 Antibody (NBP2-24787AF488). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on TLR5.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

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Recent Reviews

5
5
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1
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Anonymous
05/30/2017
Application: ICC
Species: Human

Bioinformatics

Gene Symbol TLR5
Entrez