SOX2 Antibody

Western Blot: SOX2 Antibody [NBP2-27394] - Analysis of SOX2 in adult mouse brain using SOX2 antibody at 1:1000

Immunohistochemistry-Paraffin: SOX2 Antibody [NBP2-27394] - Staining of SOX2 in mouse embryo using the SOX2 antibody at 1:50 (100x)

Immunohistochemistry-Paraffin: SOX2 Antibody [NBP2-27394] - Analysis of SOX2 in FFPE mouse brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.

Immunohistochemistry-Paraffin: SOX2 Antibody [NBP2-27394] - Staining of SOX2 in mouse embryo using the SOX2 antibody at 1:50 (40x)

Product Discontinued

SOX2 Antibody Summary

• Immunogen: A synthetic peptide made to an internal portion of the human SOX2 protein (between residues 50-100) [UniProt# P48431]
• Localization: Nuclei and cytoplasm
• Marker: Embryonic Stem Cell Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SOX2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.05% BSA.
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for SOX2 Antibody

• ANOP3
• MCOPS3
• MGC2413
• SOX2
• SRY (sex determining region Y)-box 2
• SRY-related HMG-box gene 2
• transcription factor SOX2
• transcription factor SOX-2

Background

SOX2 (SRY-RELATED HMG-BOX GENE 2), the basic helix-loop-helix transcription factor, is indispensable for maintaining embryonic stem (ES) cell pluripotency. The gene expresses in self-renewing progenitor cells and acts to inhibit neuronal differentiation. SOX2, along with other transcription factors Oct3/4, klf4, and c-Myc are used to generate induced pluripotent stem cells from mouse fibroblasts by retrovirus mediated introduction. It is expressed throughout the human brain, including the developing hypothalamus, as well as the Rathke pouch, the developing anterior pituitary and the eye. It is necessary for the normal development of the hypothalamo-pituitary and reproductive axes in mice. Mutation of the SOX2 gene in progenitor cells leads to cerebral malformation, severe eye defects, and hypothalamo-pituitary abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOX2
• Uniprot:
  • P48431()