ROBO4 Antibody


Western Blot: Robo4 Antibody [NB110-58780] - Detection of ROBO4 in HUVEC lysate.
Immunocytochemistry/ Immunofluorescence: Robo4 Antibody [NB110-58780] - Robo4 antibody was tested in Huvec cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 more
Immunohistochemistry: Robo4 Antibody [NB110-58780] - Detection of ROBO4 in human placental epithelial cells and trophoblasts.

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
1 mg/ml

ROBO4 Antibody Summary

A synthetic peptide made to a C-terminal region of human Robo4 (within residues 900-1007). [Swiss-Prot# Q8WZ75]
Cell Membrane cytoplasmic vesicles.
Tumor Endothelial Marker
This recognizes the 85 kDa and 107 kDa isoforms of the ROBO4 human protein.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate, pH 7-8
0.1% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:500
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
This Robo4 antibody is useful for Immunohistochemistry-Paraffin and Western Blot analysis, where bands at 85 kDa and 107 kDa are observed representing different isoforms of ROBO4 protein.
Control Peptide
ROBO4 Peptide (NB110-58780PEP)

Reactivity Notes


Alternate Names for ROBO4 Antibody

  • ECSM4
  • FLJ20798
  • Magic roundabout
  • MGC133352
  • MGC133353
  • MRB
  • roundabout homolog 4
  • roundabout homolog 4, magic roundabout (Drosophila)


Robo4 (roundabout homolog 4 or magic roundabout) is a receptor for Slit proteins which implicate in angiogenesis and vascular patterning especially through facilitating inhibition of primary endothelial cell migration. It is a fairly recently identified member of Robo family and is shorter than other Robo members at both intracellular as well as extracellular domains. Robo4 plays important roles in several aspects of vascular development such as the guidance of endothelial cell migration, cell cycle regulation, inhibition of pathologic angiogenesis and endothelial hyperpermeability etc. Employing mouse and zebrafish models, Slit2/Robo4 interactions have been shown to maintain integrity of vascular network and its barrier function by inhibiting cytokine-mediated vasculogenesis with enhanced permeability, and that Slit2-Robo4-paxillin-GIT1 network inhibits neovascularization and vascular leakage. Moreover, single nucleotide polymorphorism of Robo4 has been documented to be associated with autism suggesting its role in development and function of human brain.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for ROBO4 Antibody (NB110-58780): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB110-58780AF405
Alexa Fluor (R) 488 Labeled NB110-58780AF488
Alexa Fluor (R) 647 Labeled NB110-58780AF647
Alexa Fluor (R) 700 Labeled NB110-58780AF700
Biotin Labeled NB110-58780B
DyLight 405 Labeled NB110-58780V
DyLight 488 Labeled NB110-58780G
DyLight 550 Labeled NB110-58780R
DyLight 650 Labeled NB110-58780C
FITC Labeled NB110-58780F
HRP Labeled NB110-58780H

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Blogs on ROBO4

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Contact Information

Product PDFs


Gene Symbol ROBO4

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