ROBO4 Antibody

Images

 
Western Blot: Robo4 Antibody [NB110-58780] - Detection of ROBO4 in HUVEC lysate.
Immunocytochemistry/ Immunofluorescence: Robo4 Antibody [NB110-58780] - Robo4 antibody was tested in Huvec cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: Robo4 Antibody [NB110-58780] - Detection of ROBO4 in human placental epithelial cells and trophoblasts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

ROBO4 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal region of human Robo4 (within residues 900-1007). [Swiss-Prot# Q8WZ75]
Localization
Cell Membrane, cytoplasmic vesicles.
Marker
Tumor Endothelial Marker
Specificity
This recognizes the 85 kDa and 107 kDa isoforms of the ROBO4 human protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ROBO4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
This Robo4 antibody is useful for Immunohistochemistry-Paraffin and Western Blot analysis, where bands at 85 kDa and 107 kDa are observed representing different isoforms of ROBO4 protein.
Positive Control
ROBO4 Lysate (NBL1-15473)
Control Peptide
ROBO4 Peptide (NB110-58780PEP)

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ROBO4 Antibody

  • ECSM4
  • FLJ20798
  • Magic roundabout
  • MGC133352
  • MGC133353
  • MRB
  • ROBO4
  • roundabout homolog 4
  • roundabout homolog 4, magic roundabout (Drosophila)

Background

Robo4 (roundabout homolog 4 or magic roundabout) is a receptor for Slit proteins which implicate in angiogenesis and vascular patterning especially through facilitating inhibition of primary endothelial cell migration. It is a fairly recently identified member of Robo family and is shorter than other Robo members at both intracellular as well as extracellular domains. Robo4 plays important roles in several aspects of vascular development such as the guidance of endothelial cell migration, cell cycle regulation, inhibition of pathologic angiogenesis and endothelial hyperpermeability etc. Employing mouse and zebrafish models, Slit2/Robo4 interactions have been shown to maintain integrity of vascular network and its barrier function by inhibiting cytokine-mediated vasculogenesis with enhanced permeability, and that Slit2-Robo4-paxillin-GIT1 network inhibits neovascularization and vascular leakage. Moreover, single nucleotide polymorphorism of Robo4 has been documented to be associated with autism suggesting its role in development and function of human brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ROBO4 Antibody (NB110-58780) (0)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ROBO4 Antibody (NB110-58780) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-58780AF405
Alexa Fluor 488 Labeled NB110-58780AF488
Alexa Fluor 647 Labeled NB110-58780AF647
Alexa Fluor 700 Labeled NB110-58780AF700
Biotin Labeled NB110-58780B
DyLight 405 Labeled NB110-58780V
DyLight 488 Labeled NB110-58780G
DyLight 550 Labeled NB110-58780R
DyLight 650 Labeled NB110-58780C
FITC Labeled NB110-58780F
HRP Labeled NB110-58780H

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Bioinformatics

Gene Symbol ROBO4
Entrez
Uniprot