ROBO4 Antibody [Biotin]

HUVEC cells were stained with Goat Anti-Human ROBO4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2366, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram) followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

ROBO4 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ROBO4
  Gln28-Arg467
  Accession # Q8WZ75
• Specificity: Detects human ROBO4 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant rat ROBO1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ROBO4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROBO4 Antibody [Biotin]

• ECSM4
• FLJ20798
• Magic roundabout
• MGC133352
• MGC133353
• MRB
• ROBO4
• roundabout homolog 4
• roundabout homolog 4, magic roundabout (Drosophila)

Background

ROBO4 belongs to the ROBO family, which are molecular guidance receptors that interact with Slit ligand to regulate axon guidance, neuronal migration and leukocyte chemotaxis. The expression of ROBO4 is restricted in the endothelium, notably in areas of angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROBO4 Antibody (BAF2366)(2)

Publications using BAF2366

• The Robo4 cytoplasmic domain is dispensable for vascular permeability and neovascularization Nat Commun, 2016-11-24;7(0):13517. 2016-11-24 [PMID: 27882935] (ELISA Development, Mouse)
• Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis. Exp. Cell Res., 2011-12-14;318(4):371-8. 2011-12-14 [PMID: 22198087] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ROBO4
• Uniprot:
  • Q8WZ75()