ROBO4 Antibody [Unconjugated]

HUVEC cells were stained with Goat Anti-Human ROBO4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2366, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ROBO4 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ROBO4
  Gln28-Arg467
  Accession # Q8WZ75
• Specificity: Detects human ROBO4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant rat ROBO1, recombinant human (rh) ROBO2, and rhROBO3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ROBO4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ROBO4 Antibody [Unconjugated]

• ECSM4
• FLJ20798
• Magic roundabout
• MGC133352
• MGC133353
• MRB
• ROBO4
• roundabout homolog 4
• roundabout homolog 4, magic roundabout (Drosophila)

Background

ROBO4 belongs to the ROBO family, which are molecular guidance receptors that interact with Slit ligand to regulate axon guidance, neuronal migration and leukocyte chemotaxis. The expression of ROBO4 is restricted in the endothelium, notably in areas of angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ROBO4 Antibody (AF2366)(2)

Publications using AF2366

• A Pircher, G Schäfer, A Eigentler, R Pichler, M Puhr, E Steiner, W Horninger, E Gunsilius, H Klocker, I Heidegger Robo 4 - the double-edged sword in prostate cancer: impact on cancer cell aggressiveness and tumor vasculature Int J Med Sci, 2019-01-01;16(1):115-124. 2019-01-01 [PMID: 30662335] (Western Blot, Human)
• K Shirakura, R Ishiba, T Kashio, R Funatsu, T Tanaka, SI Fukada, K Ishimoto, N Hino, M Kondoh, Y Ago, Y Fujio, K Yano, T Doi, WC Aird, Y Okada The Robo4-TRAF7 complex suppresses endothelial hyperpermeability in inflammation J. Cell. Sci., 2019-01-02;0(0):. 1/2/2019 [PMID: 30510113]

Bioinformatics

• Gene Symbol: ROBO4
• Uniprot:
  • Q8WZ75()