PUMA Antibody

Images

 
Western Blot: PUMA Antibody [NB500-261] - Detection of the beta isoform of PUMA in HL-60 whole cell lysate using NB 500-261(4 ug/ml). ECL detection at a 30 minute exposure.
Immunocytochemistry/ Immunofluorescence: PUMA Antibody [NB500-261] - The PUMA antibody was tested at a 1:20 dilution in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
5.65 mg/ml

PUMA Antibody Summary

Immunogen
A synthetic peptide made to a region within the C-terminus of the human PUMA protein sequence (between residues 150-193). [UniProt# Q9BXH1]
Localization
Mitochondrial
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BBC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:20-1:100
Application Notes
This PUMA antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. By WB a band at ~16 kDa is seen, representing the beta form of PUMA. Reactivity against the alpha form of PUMA is unknown.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
PUMA Lysate (NBL1-07925)
Control Peptide
PUMA Peptide (NB500-261PEP)
Publications
Read Publication using
NB500-261 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
5.65 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PUMA Antibody

  • BCL2 binding component 3
  • bcl-2-binding component 3
  • JFY1
  • JFY-1
  • p53 up-regulated modulator of apoptosis
  • PUMAFLJ42994

Background

PUMA (p53 upregulated modulator of apoptosis) belongs to pro-apoptotic members of Bcl2 family's BH3-only subgroup, and besides its most critical role as mediator of p53/TP53-dependent/-independent apoptosis, it acts as a regulator of ER stress-induced neuronal apoptosis also. PUMA is expressed ubiquitously and predominately localizes to mitochondria where it execute induction of cytochrome c release. PUMA mediates apoptosis via direct binding with anti-apoptotic members of the Bcl-2 family and is capable of interacting with MCL1, BCL2A1, BCL2 and BCL2L1/BCL-XL. PUMA binding to inhibitory members of Bcl-2 family (Bcl-2-like proteins) leads to Bax and/or Bak displacement mediated activation and formation of pore-like structures over mitochondrial membrane, which permeabilizes the outer mitochondrial membrane, resulting in mitochondrial dysfunction and caspase activation. PUMA is induced by p53, DNA damage, glucocorticoid treatment, deprivation of growth factors etc. and through ER stress in a DDIT3/CHOP-dependent manner. PUMA has been reported to involve in multiple physiological and pathological processes, including immune response, cancer, neurodegenerative diseases and bacterial as well as viral infections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PUMA Antibody (NB500-261)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

View specific protocols for PUMA Antibody (NB500-261): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PUMA Antibody (NB500-261) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-261AF405
Alexa Fluor 488 Labeled NB500-261AF488
Alexa Fluor 647 Labeled NB500-261AF647
Alexa Fluor 700 Labeled NB500-261AF700
Biotin Labeled NB500-261B
DyLight 405 Labeled NB500-261V
DyLight 488 Labeled NB500-261G
DyLight 550 Labeled NB500-261R
DyLight 650 Labeled NB500-261C
FITC Labeled NB500-261F
HRP Labeled NB500-261H

Additional PUMA Products

Bioinformatics Tool for PUMA Antibody (NB500-261)

Discover related pathways, diseases and genes to PUMA Antibody (NB500-261). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for PUMA Antibody (NB500-261)

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Pathways for PUMA Antibody (NB500-261)

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Research Areas for PUMA Antibody (NB500-261)

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Blogs on PUMA.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

Understanding Noxa Regulation of Apoptosis
Noxa is a pro-apoptotic gene belonging to the Bcl2 protein family that is unique in that it contains only BH3 domain. The BH3-only subclass of proteins, including proteins like PUMA and Bim in addition to Noxa, regulate the remaining Bcl-2 family memb...  Read full blog post.

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Bioinformatics

Gene Symbol BBC3