Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1-4 µg/mL of Human BAK Antigen Affinity-purified Polyclonal ...read more
BAK was detected in immersion fixed HEK293 human embryonic kidney cell line using Rabbit Anti-Human BAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF816) at 15 µg/mL for 3 hours at room ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for BAK Antibody [Unconjugated]
Apoptosis regulator BAK
BCL2L7bcl-2 homologous antagonist/killer
BCL2-like 7 protein
Bcl-2-like protein 7
pro-apoptotic protein BAK
BAK (Bcl-2 homologous antagonist/killer; also BAK1) is a 25‑30 kDa member of the BCL-2 family of proteins. It is widely expressed, and participates in the apoptotic cycle. BAK is an outer mitochondrial membrane protein that is inactive as a Zn-dependent homodimer. Upon activation by p53 or tBID, BAK oligomerizes, creating a pore in the mitochondrial membrane and allowing for cytochrome C release. Human BAK is 211 amino acids (aa) in length and contains three BCL-2 homology domains (aa 74‑88, 117‑136 and 169‑184), a Zn-binding region (aa 160‑166) and a C-terminal transmembrane segment (aa 188‑205). Amino acids 67‑94 mediate oligomerization of BAK. There are two potential isoform variants; one shows an alternate start site at Met96, while a second shows a deletion of aa 46‑66. Over amino acids 53-72, human BAK shares 55% aa identity with mouse BAK.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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